Cited by Optimizing CAR-T cell Culture: Differential effects of IL-2, IL-12, and IL-21 on CAR-T cells

Enhancing precision in cancer treatment: the role of gene therapy and immune modulation in oncology DOI

Emile M. Youssef,

Brandon Fletcher,

Dannelle Palmer

et al.

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 11

Published: Jan. 13, 2025

Gene therapy has long been a cornerstone in the treatment of rare diseases and genetic disorders, offering targeted solutions to conditions once considered untreatable. As field advances, its transformative potential is now expanding into oncology, where personalized therapies address immune-related complexities cancer. This review highlights innovative therapeutic strategies, including gene replacement, silencing, oncolytic virotherapy, CAR-T cell therapy, CRISPR-Cas9 editing, with focus on their application both hematologic malignancies solid tumors. CRISPR-Cas9, revolutionary tool precision medicine, enables precise editing cancer-driving mutations, enhancing immune responses disrupting tumor growth mechanisms. Additionally, emerging approaches target ferroptosis—a regulated, iron-dependent form death—offering new possibilities for selectively inducing death resistant cancers. Despite significant breakthroughs, challenges such as heterogeneity, evasion, immunosuppressive microenvironment (TME) remain. To overcome these barriers, novel like dual-targeting, armored cells, combination checkpoint inhibitors ferroptosis inducers are being explored. rise allogeneic “off-the-shelf” offers scalable more accessible options. The regulatory landscape evolving accommodate advancements, frameworks RMAT (Regenerative Medicine Advanced Therapy) U.S. ATMP (Advanced Therapy Medicinal Products) Europe fast-tracking approval therapies. However, ethical considerations surrounding CRISPR-based editing—such off-target effects, germline ensuring equitable access—remain at forefront, requiring ongoing oversight. Advances non-viral delivery systems, lipid nanoparticles (LNPs) exosomes, improving safety efficacy By integrating innovations addressing concerns, poised revolutionize cancer treatment, providing durable, effective,

Language: Английский

Citations

Ferroptosis of T cell in inflammation and tumour immunity DOI

Xueli Xia, Haisheng Wu, Yuxuan Chen

et al.

Clinical and Translational Medicine, Journal Year: 2025, Volume and Issue: 15(3)

Published: March 1, 2025

Ferroptosis is an innovative concept defined as a distinct programmed cell death mode regulated by iron-dependent lipid peroxidation accumulation. This process governed numerous energy metabolites such fatty acids, amino acids and glucose, well iron homeostasis. In recent years, increasing studies have been devoted to the crucial effects of ferroptosis in immune cells during pathogenesis diseases infections, tumours autoimmune disorders. review summarises latest advancements T-cell ferroptosis, addresses key components mechanism T inflammatory conditions tumour progression, highlights potential target for treating related diseases. KEY POINTS: Ferroptosis-related mechanisms significantly affect biology CD4+ subsets are further involved Crosstalk between CD8+ induces microenvironment. Glutathione peroxidase 4 loss promotes regulatory enhance anti-tumour immunity.

Language: Английский

Citations

ROR1 CAR-T cells and ferroptosis inducers orchestrate tumor ferroptosis via PC-PUFA2 DOI

Dan Li, Wenjie Zhang, Ruiheng Wang

et al.

Biomarker Research, Journal Year: 2025, Volume and Issue: 13(1)

Published: Jan. 23, 2025

Language: Английский

Citations

JAK/STAT signaling as a key regulator of ferroptosis: mechanisms and therapeutic potentials in cancer and diseases DOI

Yimeng Dai,

Chunguo Cui,

Dan Jiao

et al.

Cancer Cell International, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 7, 2025

Ferroptosis is a distinct form of regulated cell death characterized by iron-dependent lipid peroxidation, playing critical role in various diseases, including cancer, neurodegeneration, and tissue damage. This study reviews the intricate relationship between ferroptosis Janus kinase/signal transducer activator transcription (JAK/STAT) signaling pathway, highlighting its regulatory functions across multiple biological processes. Dysregulation JAK/STAT pathway implicated promoting or inhibiting ferroptosis, depending on context. JAK2 promotes activating STAT proteins, modulating expression key regulators like SLC7A11 GPX4, influencing iron homeostasis through pathways such as ferritinophagy hepcidin regulation. STAT1 activation primarily enhances suppression cystine-glutamate antiporter (System Xc-), leading to glutathione depletion contributing conditions Sjogren's syndrome age-related macular degeneration. In contrast, STAT3 plays protective upregulating which inhibits survival, particularly cancers hepatocellular carcinoma, prostate renal carcinoma. also discusses STAT6's involvement diseases asthma lung injury regulating antioxidant defenses. Furthermore, review explores potential therapeutic strategies targeting manipulate for disease treatment. cancer therapy, this can enhance effectiveness inducers, offering promising avenues overcome drug resistance. Additionally, interplay immune responses, oxidative stress, metabolism underscores significance progression intervention. By exploring these mechanisms, provides insights into development novel treatments modulation, with implications inflammatory neurodegenerative conditions.

Language: Английский

Citations

Metal ions-induced programmed cell death: how does oxidative stress regulate cell death? DOI

Xingsheng Wang,

Zuohao Liu,

Changjun Lin

et al.

Life Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 123688 - 123688

Published: May 1, 2025

Language: Английский

Citations

Peripheral blood–derived PD-1/CD28–CD19-CAR–modified PD-1+ T-cell therapy in patients with solid tumors DOI

Zhen Zhang,

Xuan Zhao,

Qitai Zhao

et al.

Cancer Immunology Research, Journal Year: 2024, Volume and Issue: 12(12), P. 1703 - 1717

Published: Sept. 16, 2024

Abstract T cells expressing programmed cell death 1 (PD-1) in the peripheral blood (PB) of patients with tumors possess therapeutic potential; however, immunosuppressive, PD-1–triggered signaling pathway and limited proliferative capacity PD-1+ present challenges to their application. Here, we observed no discernible distinction between PD-1− terms clonal overlap. However, CD8+PD-1+ from PB tumor tissues exhibited tighter clustering based on clone size. Single-cell RNA sequencing analysis showed that highly expressed cytotoxicity-related genes were enriched for T-cell activation–related pathways compared or tissues. Consistent this, PB-derived strong cytotoxicity toward autologous lines. To augment activity against solid vivo, introduced a PD-1/CD28 fusion receptor combined CD19 chimeric antigen into cells, which then expanded vitro. The modified superior proliferation antitumor abilities In addition, four cancer infused PD-1/CD28–CD19 cells. None these experienced severe side effects, one patient melanoma achieved complete response was maintained 6.7 months. three other had stable disease. Collectively, results suggested therapy is both safe effective, it may constitute promising treatment strategy cancer.

Language: Английский

Citations

Optimizing CAR-T cell Culture: Differential effects of IL-2, IL-12, and IL-21 on CAR-T cells DOI

Mengmeng Zhang, Jingjing Kong,

Fanxiang Yin

et al.

Cytokine, Journal Year: 2024, Volume and Issue: 184, P. 156758 - 156758

Published: Sept. 17, 2024

Language: Английский

Citations