Non-coding RNAs: emerging players in cardiomyocyte proliferation and cardiac regeneration DOI Creative Commons
Naisam Abbas, Filippo Perbellini, Thomas Thum

et al.

Basic Research in Cardiology, Journal Year: 2020, Volume and Issue: 115(5)

Published: Aug. 3, 2020

Abstract Soon after birth, the regenerative capacity of mammalian heart is lost, cardiomyocytes withdraw from cell cycle and demonstrate a minimal proliferation rate. Despite improved treatment reperfusion strategies, uncompensated cardiomyocyte loss during injury disease results in cardiac remodeling subsequent failure. The promising field medicine aims to restore both structure function damaged tissue through modulation cellular processes regulatory mechanisms involved arrest boost proliferation. Non-coding RNAs (ncRNAs), such as microRNAs (miRNAs), long non-coding (lncRNAs), circular (circRNAs) are functional RNA molecules with no protein-coding that have been reported engage regeneration repair. In this review, we summarize current understanding biological functions molecular ncRNAs Furthermore, discuss their impact on contractile health application for therapeutic interventions.

Language: Английский

miRNA-based biomarkers, therapies, and resistance in Cancer DOI Creative Commons
Boxue He, Zhenyu Zhao, Qidong Cai

et al.

International Journal of Biological Sciences, Journal Year: 2020, Volume and Issue: 16(14), P. 2628 - 2647

Published: Jan. 1, 2020

MicroRNAs (miRNAs), small non-coding RNAs (ncRNAs) of about 22 nucleotides in size, play important roles gene regulation, and their dysregulation is implicated human diseases including cancer.A variety miRNAs could take the cancer progression, participate process tumor immune, function with miRNA sponges.During last two decades, connection between various cancers has been widely researched.Based on evidence miRNA, numerous potential biomarkers for diagnosis prognosis have put forward, providing a new perspective screening.Besides, there are several miRNA-based therapies among different being conducted, advanced treatments such as combination synergistic strategies use complementary provide significant clinical benefits to patients potentially.Furthermore, it demonstrated that many engaged resistance complex underlying regulatory mechanisms, whose comprehensive cognition can help clinicians improve patient prognosis.With belief studies would great implications, we attempt summarize current situation development prospects this review.

Language: Английский

Citations

460
Non-coding RNAs in disease: from mechanisms to therapeutics DOI
Kinga Németh, Recep Bayraktar, Manuela Ferracin

et al.

Nature Reviews Genetics, Journal Year: 2023, Volume and Issue: 25(3), P. 211 - 232

Published: Nov. 15, 2023

Language: Английский

Citations

361
Diffuse myocardial fibrosis: mechanisms, diagnosis and therapeutic approaches DOI
Begoña López, Susana Ravassa, María U. Moreno

et al.

Nature Reviews Cardiology, Journal Year: 2021, Volume and Issue: 18(7), P. 479 - 498

Published: Feb. 10, 2021

Language: Английский

Citations

229
Epigenetic regulation in cardiovascular disease: mechanisms and advances in clinical trials DOI Creative Commons

Yuncong Shi,

Huanji Zhang, Suli Huang

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2022, Volume and Issue: 7(1)

Published: June 25, 2022

Abstract Epigenetics is closely related to cardiovascular diseases. Genome-wide linkage and association analyses candidate gene approaches illustrate the multigenic complexity of disease. Several epigenetic mechanisms, such as DNA methylation, histone modification, noncoding RNA, which are importance for disease development regression. Targeting key enzymes, especially methyltransferases, acetylases, deacetylases their regulated target genes, could represent an attractive new route diagnosis treatment Herein, we summarize knowledge on history essential regulatory mechanisms in Furthermore, discuss preclinical studies drugs that targeted these enzymes diseases therapy. Finally, conclude clinical trials going some processes.

Language: Английский

Citations

215
MicroRNAs as therapeutic targets in cardiovascular disease DOI Creative Commons

Bernhard Laggerbauer,

Stefan Engelhardt

Journal of Clinical Investigation, Journal Year: 2022, Volume and Issue: 132(11)

Published: May 31, 2022

The discovery of microRNAs and their role in diseases was a breakthrough that inspired research into as drug targets. Cardiovascular are an area which limitations conventional pharmacotherapy highly apparent where microRNA-based drugs have appreciably progressed preclinical clinical testing. In this Review, we summarize the current state therapeutic targets cardiovascular system. We report recent advances identification characterization microRNAs, manipulation translation, discuss challenges perspectives toward application.

Language: Английский

Citations

110
Nanotechnology for cardiovascular diseases DOI
Qinqin Hu,

Zheyan Fang,

Junbo Ge

et al.

The Innovation, Journal Year: 2022, Volume and Issue: 3(2), P. 100214 - 100214

Published: Feb. 2, 2022

Language: Английский

Citations

77
A circular RNA derived from the insulin receptor locus protects against doxorubicin-induced cardiotoxicity DOI Creative Commons
Dongchao Lu, Shambhabi Chatterjee, Ke Xiao

et al.

European Heart Journal, Journal Year: 2022, Volume and Issue: 43(42), P. 4496 - 4511

Published: June 27, 2022

Cardiotoxicity leading to heart failure (HF) is a growing problem in many cancer survivors. As specific treatment strategies are not available, RNA discovery pipelines were employed and new powerful circular (circRNA)-based therapy was developed for the of doxorubicin-induced HF.The circRNA sequencing applied highly species-conserved insulin receptor (Circ-INSR) identified, which participates HF processes, including those provoked by cardiotoxic anti-cancer treatments. Chemotherapy-provoked cardiotoxicity leads down-regulation Circ-INSR rodents patients, mechanistically contributes cardiomyocyte cell death, cardiac dysfunction, mitochondrial damage. In contrast, overexpression prevented doxorubicin-mediated both rodent human cardiomyocytes vitro mouse model chronic doxorubicin cardiotoxicity. Breast type 1 susceptibility protein (Brca1) identified as regulator expression. Detailed transcriptomic proteomic analyses revealed that regulates apoptotic metabolic pathways cardiomyocytes. physically interacts with single-stranded DNA-binding (SSBP1) mediating its cardioprotective effects under stress. Importantly, transcribed circularized mimics also protected against cardiotoxicity.Circ-INSR conserved non-coding down-regulated during remodelling. Adeno-associated virus mimics-based prevent reverse death improve function. The results this study highlight novel translationally important Circ-INSR-based therapeutic approach dysfunction.

Language: Английский

Citations

73
Artificial intelligence framework for heart disease classification from audio signals DOI Creative Commons
Sidra Abbas, Stephen Ojo, Abdullah Al Hejaili

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Feb. 7, 2024

Abstract As cardiovascular disorders are prevalent, there is a growing demand for reliable and precise diagnostic methods within this domain. Audio signal-based heart disease detection promising area of research that leverages sound signals generated by the to identify diagnose disorders. Machine learning (ML) deep (DL) techniques pivotal in classifying identifying from audio signals. This study investigates ML DL detect analyzing noisy employed two subsets datasets PASCAL CHALLENGE having real audios. The process visually depict using spectrograms Mel-Frequency Cepstral Coefficients (MFCCs). We employ data augmentation improve model’s performance introducing synthetic noise In addition, feature ensembler developed integrate various extraction techniques. Several machine classifiers utilized detection. Among numerous models studied previous findings, multilayer perceptron model performed best, with an accuracy rate 95.65%. demonstrates potential methodology accurately detecting These findings present opportunities enhancing medical diagnosis patient care.

Language: Английский

Citations

18
Physiological Biomimetic Culture System for Pig and Human Heart Slices DOI Open Access

Qinghui Ou,

Zoë Jacobson,

Riham Abouleisa

et al.

Circulation Research, Journal Year: 2019, Volume and Issue: 125(6), P. 628 - 642

Published: July 16, 2019

Preclinical testing of cardiotoxicity and efficacy novel heart failure therapies faces a major limitation: the lack an in situ culture system that emulates complexity human tissue maintains viability functionality for prolonged time. To develop reliable, easily reproducible, medium-throughput method to pig slices under physiological conditions period Here, we describe novel, biomimetic (300 µm thickness) 6 days culture. We optimized medium with continuous electrical stimulation at 1.2 Hz oxygenation medium. Functional these over was confirmed by assessing their calcium homeostasis, twitch force generation, response β-adrenergic stimulation. Temporal transcriptome analysis using RNAseq day 2, 6, 10 overall maintenance normal gene expression up days, while 500 transcripts were differentially regulated after days. Electron microscopy demonstrated intact mitochondria Z-disc ultra-structures our conditions. This successful keeping completely viable functionally structurally also used this demonstrate effects therapy approach slices. Furthermore, enabled assessment contraction relaxation kinetics on isolated single myofibrils from have developed reliable as platform therapeutics 3-dimensional model.

Language: Английский

Citations

81
MicroRNA-34a: the bad guy in age-related vascular diseases DOI Creative Commons
Angela Raucci, Federica Macrì, Stefania Castiglione

et al.

Cellular and Molecular Life Sciences, Journal Year: 2021, Volume and Issue: 78(23), P. 7355 - 7378

Published: Oct. 26, 2021

Abstract The age-related vasculature alteration is the prominent risk factor for vascular diseases (VD), namely, atherosclerosis, abdominal aortic aneurysm, calcification (VC) and pulmonary arterial hypertension (PAH). chronic sterile low-grade inflammation state, alias inflammaging , characterizes elderly people participates in VD development. MicroRNA34-a (miR-34a) emerging as an important mediator of VD. miR-34a increases with aging vessels induces senescence acquisition senescence-associated secretory phenotype (SASP) smooth muscle (VSMCs) endothelial (ECs) cells. Similarly, other factors, including dyslipidemia, hyperglycemia hypertension, modify expression to promote inflammation. upregulation causes dysfunction by affecting ECs nitric oxide bioavailability, adhesion molecules inflammatory cells recruitment. miR-34a-induced facilitates VSMCs osteoblastic switch VC development hyperphosphatemia conditions. Conversely, atherogenic hypoxic stimuli downregulate levels proliferation migration during atherosclerosis PAH. MiR34a genetic ablation or inhibition anti-miR-34a different experimental models reduce inflammation, apoptosis through sirtuin 1 Notch1, B-cell lymphoma 2 modulation. Notably, pleiotropic drugs, like statins, liraglutide metformin, affect expression. Finally, human studies report that associate diabetes correlate factors aging. Herein, we comprehensively review current knowledge about miR-34a-dependent molecular cellular mechanisms activated highlight diagnostic therapeutic potential modulating its order burn extend healthy lifespan.

Language: Английский

Citations

77