Cellular Signalling, Journal Year: 2023, Volume and Issue: 109, P. 110744 - 110744
Published: June 2, 2023
Language: Английский
Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)
Published: March 15, 2025
Myocardial infarction (MI) compromises the cardiac microvascular endothelial barrier, increasing leakage and inflammation. HIF2α, predominantly expressed in cells during ischemia, has an unclear role barrier function MI. Here, we show that inducible, adult endothelial-specific deletion of Hif2α mice leads to increased mortality, leakage, inflammation, reduced heart function, adverse remodeling after In parallel, human (HCMVECs) lacking HIF2α display impaired integrity, tight-junction proteins, cell death, elevated IL-6 levels, effects are alleviated by overexpressing ARNT, a key partner under hypoxic conditions. Interestingly, but not directly binds promoter suppress its expression. These findings suggest HIF2α/ARNT axis as protective mechanism failure post-MI identify potential therapeutic targets support function. Endothelial-specific results remodelling post-myocardial mice, highlighting HIF2α‒ARNT failure.
Language: Английский
Citations
0
Mechanobiology in Medicine, Journal Year: 2025, Volume and Issue: unknown, P. 100127 - 100127
Published: March 1, 2025
Language: Английский
Citations
0
Frontiers in Bioscience-Landmark, Journal Year: 2025, Volume and Issue: 30(5)
Published: April 30, 2025
Lysophosphatidic acid (LPA), a bioactive lipid molecule, has been identified as critical regulator of several cellular processes in the central nervous system, with significant impacts on neuronal function, synaptic plasticity, and neuroinflammatory responses. While Alzheimer’s disease, Multiple Sclerosis, Parkinson’s disease have garnered considerable attention due to their incidence socioeconomic significance, many additional neurological illnesses remain unclear terms underlying pathophysiology prospective treatment targets. This review synthesizes evidence linking LPA’s function diseases such traumatic brain injury, spinal cord cerebellar ataxia, cerebral ischemia, seizures, Huntington’s amyotrophic lateral sclerosis, Hutchinson-Gilford progeria syndrome, autism, migraine, human immunodeficiency virus (HIV)-associated complications Despite recent advances, specific mechanisms actions various disorders unknown, further research is needed understand distinct roles LPA across multiple conditions, well investigate therapeutic potential targeting receptors these pathologies. The purpose this highlight functions aforementioned diseases, which frequently share same poor prognosis scarcity truly effective therapies, while also evaluating role LPA, its receptors, signaling promising actors for development alternative strategies those proposed today.
Language: Английский
Citations
0
International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 347 - 366
Published: Jan. 1, 2024
Introduction: Excessive generation of reactive oxygen species (ROS) following myocardial ischemia-reperfusion (I/R) can result in additional death cells. The rapid clearance ROS after reperfusion injury and intervention during subsequent cardiac repair stages are crucial for the ultimate recovery function. Methods: Magnesium-doped mesoporous bioactive glasses were prepared loaded with antioxidant drug gallic acid into MgNPs by sol-gel method. effects MgNPs/GA tested their pro-angiogenic anti-inflammatory based on release characteristics GA Mg 2+ from MgNPs/GA. Later, we confirmed our vivo tests through immunofluorescence staining tissue sections at various time points that exhibited initial had both phase. Finally, evaluated function mice treated Results: We provide evidence released effectively eliminate early stage, decreasing cell apoptosis. During phase, gradual stimulated angiogenesis promoted M2 macrophage polarization, thereby reducing inflammatory factors. Conclusion: acting multiple types is an integrated solution comprehensive attenuation ischaemia-reperfusion protection. Keywords: ischemia/reperfusion injury, glass, acid, magnesium ion, polarization
Language: Английский
Citations
3
Basic Research in Cardiology, Journal Year: 2024, Volume and Issue: 119(2), P. 329 - 348
Published: Jan. 18, 2024
Language: Английский
Citations
3
ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(11), P. 13466 - 13480
Published: March 6, 2024
Guided bone regeneration (GBR) technology has been widely used for the of periodontal defects. However, limited mechanical properties and potential currently available GBR membranes often limit their repair effectiveness. In this paper, serum-derived growth factor lysophosphatidic acid (LPA) nanoparticles dopamine-decorative nanohydroxyapatite (pDA/nHA) particles were double-loaded into polylactic-glycolic acid/polycaprolactone (PLGA/PCL) scaffolds as an organic/inorganic biphase delivery system, namely, PP-pDA/nHA-LPA scaffolds. Physicochemical osteogenic ability in vitro vivo performed. Scanning electron microscopy tests showed that had a 3D bionic scaffold structure with improved properties. cell experiments demonstrated could significantly enhance attachment, proliferation, differentiation, mineralization MC3T3-E1 cells. vivo, exhibited great cytocompatibility recruitment 2- 4-week subcutaneous implantation promoted defect experiment. Moreover, LPA-loaded confirmed to activities by upregulating expression β-catenin further activating Wnt/β-catenin pathway. These results demonstrate system is promising material GBR.
Language: Английский
Citations
3
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: Sept. 12, 2024
Advancements in human-engineered heart tissue have enhanced the understanding of cardiac cellular alteration. Nevertheless, a human model simulating pathological remodeling following myocardial infarction for therapeutic development remains essential. Here we develop an engineered repair that replicates phased process, including hypoxic stress, fibrosis, and electrophysiological dysfunction. Transcriptomic analysis identifies nine critical signaling pathways related to fate transitions, leading evaluation seventeen modulators their potential mini-repair model. A scoring system quantitatively evaluates restoration abnormal electrophysiology, demonstrating combination TGFβ inhibitor SB431542, Rho kinase Y27632, WNT activator CHIR99021 yields functional compared single factor treatments both mouse This effectively captures infarction, providing crucial platform discovering targets ischemic disease.
Language: Английский
Citations
3
European journal of medical research, Journal Year: 2024, Volume and Issue: 29(1)
Published: Dec. 23, 2024
Abstract The gut microbiota is a complex and dynamic ecosystem that plays crucial role in human health disease, including obesity, diabetes, cardiovascular diseases, neurodegenerative inflammatory bowel cancer. Chronic inflammation common feature of these diseases closely related to angiogenesis (the process forming new blood vessels), which often dysregulated pathological conditions. Inflammation potentially acts as central mediator. This abstract aims elucidate the connection between various diseases. influences through mechanisms, production metabolites directly or indirectly affect vascularization. For example, short-chain fatty acids (SCFAs) such butyrate, propionate, acetate are known regulate immune responses inflammation, thereby affecting angiogenesis. In context promotes atherosclerosis vascular dysfunction by producing trimethylamine N-oxide (TMAO) other promote endothelial dysfunction. Similarly, may influence neuroinflammation integrity blood–brain barrier, cases fractures wound healing, activating effects, facilitating healing tissue damage. cancer, can either inhibit tumor growth angiogenesis, depending on specific bacterial composition their metabolites. instance, some bacteria activate inflammasomes, leading factors alter microenvironment angiogenesis-related signaling pathways, metastasis. Some interact with cells, pathways. Diet, modifiable factor, significantly diet-derived microbial Diet rapidly its metabolic activity, changing concentration microbial-derived profoundly host's response high animal protein diet pro-atherogenic like TMAO, pathways interfering platelet function, associated severity coronary artery plaques, peripheral A rich dietary fiber SCFAs, act ligands for cell surface intracellular receptors, regulating biological processes, homeostasis, responses, influencing summary, multifaceted, playing an important disease progression processes multiple Diet-derived play linking Understanding interactions diet, microbiota, has potential uncover novel therapeutic targets managing Therefore, interventions targeting metabolites, fecal transplantation (FMT) application probiotics enhance beneficial present promising strategy.
Language: Английский
Citations
3
Journal of Ethnopharmacology, Journal Year: 2023, Volume and Issue: 319, P. 117252 - 117252
Published: Sept. 29, 2023
Language: Английский
Citations
7
Cardiology, Journal Year: 2024, Volume and Issue: 149(3), P. 286 - 296
Published: Jan. 1, 2024
<b><i>Introduction:</i></b> This study aimed to explore the function of miR-135a in progress atrial fibrosis and mechanism miR-135a/SIRT1 (sirtuin 1) human cardiac fibroblasts mouse (MCFs) mediating regulation by mitochondrial oxidative respiration function. <b><i>Methods:</i></b> Using Ang II (angiotensin II) induce HCFs (human corneal fibroblasts) MCF (Michigan Cancer Foundation, MCF) cells vitro, miRNA-seq results previous studies were validated. Proliferative invasive ability MCFs was detected Cell Counting Kit-8 assay (CCK-8) scratch experiment after overexpressing cells. Protein mRNA expression tested using Western blot qPCR. The target verified as SIRT1 a luciferase reporter activities respiratory enzyme complexes I, II, III, IV determined colorimetrically. malondialdehyde, reactive oxygen species, superoxide dismutase with enzyme-linked immunosorbent (ELISA). <b><i>Results:</i></b> elevated group than control group. Overexpression could promote proliferation, migration, stress, well suppresses activity. In addition, we found gene miR-135a. What is more, findings showed promoted acting through SIRT1. <b><i>Conclusions:</i></b> mediates regulate fibrosis.
Language: Английский
Citations
2