genesis,
Journal Year:
2025,
Volume and Issue:
63(2)
Published: April 1, 2025
ABSTRACT
Single‐cell
RNA
sequencing
(scRNA‐seq)
is
a
rapidly
developing
and
useful
technique
for
elucidating
biological
mechanisms
characterizing
individual
cells.
Tens
of
millions
patients
worldwide
suffer
from
heart
injuries
other
types
disease.
Neonatal
mammalian
hearts
certain
adult
vertebrate
species,
such
as
zebrafish,
can
fully
regenerate
after
myocardial
injury.
However,
the
unable
to
damaged
myocardium.
scRNA‐seq
provides
many
new
insights
into
pathological
normal
facilitates
our
understanding
cellular
responses
cardiac
injury
repair
at
different
stages,
which
may
provide
critical
clues
effective
therapies
regeneration.
In
this
review,
we
summarize
application
in
development
regeneration
describe
how
important
molecular
be
harnessed
promote
Circulation Research,
Journal Year:
2024,
Volume and Issue:
134(7), P. 931 - 949
Published: March 28, 2024
The
ECM
(extracellular
matrix)
is
a
major
component
of
the
vascular
microenvironment
that
modulates
homeostasis.
proteins
include
collagens,
elastin,
noncollagen
glycoproteins,
and
proteoglycans/glycosaminoglycans.
form
complex
matrix
structures,
such
as
basal
lamina
collagen
elastin
fibers,
through
direct
interactions
or
lysyl
oxidase-mediated
cross-linking.
Moreover,
directly
interact
with
cell
surface
receptors
extracellular
secreted
molecules,
exerting
matricellular
matricrine
modulation,
respectively.
In
addition,
proteases
degrade
cleave
proteins,
thereby
contributing
to
turnover.
These
constitute
interactome
network,
which
essential
for
maintaining
homeostasis
preventing
pathological
remodeling.
current
review
mainly
focuses
on
endogenous
in
blood
vessels
discusses
interaction
these
other
receptors,
cytokines,
complement
coagulation
factors,
their
potential
roles
Exercise
can
stimulate
physiological
cardiac
growth
and
provide
cardioprotection
effect
in
ischemia/reperfusion
(I/R)
injury.
MiR-210
is
regulated
the
adaptation
process
induced
by
exercise;
however,
its
impact
on
exercise-induced
contribution
to
exercise-driven
remain
unclear.
We
investigated
role
mechanism
of
miR-210
explored
whether
contributes
protection
alleviating
I/R
Here,
we
first
observed
that
regular
swimming
exercise
markedly
increase
levels
heart
blood
samples
rats
mice.
Circulating
were
also
elevated
after
a
programmed
rehabilitation
patients
diagnosed
coronary
diseases.
In
8-week
model
wild-type
(WT)
knockout
(KO)
rats,
demonstrated
was
not
integral
for
hypertrophy
but
it
did
influence
cardiomyocyte
proliferative
activity.
neonatal
rat
cardiomyocytes,
promoted
cell
proliferation
suppressed
apoptosis
while
altering
size.
Additionally,
survival
human
embryonic
stem
cell-derived
cardiomyocytes
(hESC-CMs)
AC16
line,
indicating
functional
roles
cardiomyocytes.
further
identified
target
genes,
cyclin-dependent
kinase
10
(CDK10)
ephrin-A3
(EFNA3),
regulate
apoptosis.
Finally,
KO
WT
subjected
followed
crucially
contributed
against
summary,
this
study
elucidates
miR-210,
an
exercise-responsive
miRNA,
promoting
activity
during
growth.
Furthermore,
plays
essential
mediating
protective
effects
Our
findings
suggest
as
potent
nonpharmaceutical
intervention
inducing
which
alleviate
injury
promote
cardioprotection.
The Journal of Cardiovascular Aging,
Journal Year:
2024,
Volume and Issue:
4(2)
Published: June 3, 2024
Cellular
senescence
in
cardiomyocytes,
characterized
by
cell
cycle
arrest,
resistance
to
apoptosis,
and
the
senescence-associated
secretory
phenotype,
occurs
during
aging
response
various
stresses,
such
as
hypoxia/reoxygenation,
ischemia/reperfusion,
myocardial
infarction
(MI),
pressure
overload,
doxorubicin
treatment,
angiotensin
II,
diabetes,
thoracic
irradiation.
Senescence
heart
has
both
beneficial
detrimental
effects.
Premature
of
myofibroblasts
salutary
effects
MI
overload.
On
other
hand,
persistent
activation
cardiomyocytes
precipitates
cardiac
dysfunction
adverse
remodeling
through
paracrine
mechanisms
MI,
aging,
doxorubicin-induced
cardiomyopathy.
Given
roles
many
conditions,
specific
removal
senescent
cells,
i.e.,
senolysis,
is
great
interest.
Senolysis
can
be
achieved
using
senolytic
drugs
(such
Navitoclax,
Dasatinib,
Quercetin),
pharmacogenetic
approaches
(including
INK-ATTAC
AP20187,
p16-3MR
Ganciclovir,
p16
ablation,
p16-LOX-ATTAC
Cre),
immunogenetic
interventions
(CAR
T
cells
or
vaccination).
In
order
enhance
specificity
decrease
off-target
approaches,
investigation
into
which
develop
and/or
maintain
state
needed.
Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 7, 2025
Sustained
β-adrenergic
activation
induces
cardiac
fibrosis
characterized
by
excessive
deposition
of
extracellular
matrix
(ECM).
Prostaglandin
E2
(PGE2)
receptor
EP4
is
essential
for
cardiovascular
homeostasis.
This
study
aims
to
investigate
the
roles
cardiomyocyte
(CM)
and
fibroblast
(CF)
in
isoproterenol
(ISO)-induced
fibrosis.
By
crossing
EP4f/f
mice
with
α-MyHC-Cre
or
S100A4-Cre
mice,
this
work
obtains
CM-EP4
knockout
(EP4f/f-α-MyHCCre+)
CF-EP4
(EP4f/f-S100A4Cre+)
mice.
The
both
genders
are
subcutaneously
injected
ISO
(5
mg
kg-1
day-1)
7
days.
Compared
control
EP4f/f-α-MyHCCre+
EP4f/f-S100A4Cre+
show
a
significant
improvement
diastolic
function
as
assessed
echocardiography
histological
staining,
respectively.
In
CMs,
inhibition
suppresses
ISO-induced
TGF-β1
expression
via
blocking
cAMP/PKA
pathway.
CFs,
reversed
TGF-β1-triggers
production
ECM
preventing
formation
TGF-β1/TGF-β
complex
blocks
CF
proliferation
suppressing
ERK1/2
Furthermore,
double
CM-
administration
antagonist,
grapiprant,
markedly
improves
dysfunction
Collectively,
demonstrates
that
contribute
activation-induced
Targeting
may
offer
novel
therapeutic
approach
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(6), P. 2667 - 2667
Published: March 16, 2025
Cardiovascular
disease
(CVD)
is
a
serious
global
health
issue
with
high
mortality
rates
worldwide.
Despite
the
numerous
advancements
in
study
of
CVD
pathogenesis
recent
years,
further
summarization
and
elaboration
specific
molecular
pathways
are
required.
An
extensive
body
research
has
been
conducted
to
elucidate
association
between
MAPK
signaling
pathway,
which
present
all
eukaryotic
organisms,
cardiovascular
disease.
This
review
aims
provide
comprehensive
summary
on
over
past
five
years.
The
primary
focus
four
diseases:
heart
failure,
atherosclerosis,
myocardial
ischemia–reperfusion
injury,
cardiac
hypertrophy.
will
also
address
pathophysiological
mechanisms
diseases,
objective
proposing
novel
clinical
treatment
strategies
for
CVD.
Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 27, 2025
Innovative
therapeutic
approaches
for
heart
failure,
a
leading
cause
of
mortality
worldwide,
are
urgently
needed.
In
this
study,
the
important
role
Krüppel-like
factor
1
(KLF1)
in
cardiomyocyte
proliferation
and
regeneration
is
explored,
revealed
its
ability
to
regulate
Wnt/β-catenin
signaling
pathway
as
well
exploring
feasible
strategy
target
KLF1
treatment
failure.
Postnatally,
marked
decrease
expression
occurred
almost
simultaneously
with
reduction
myocardial
regenerative
capacity.
Through
comprehensive
vivo
vitro
studies,
it
demonstrated
that
neonatal
adult
mice,
overexpression
significantly
increased
promoted
repair
following
infarction,
whereas
knockout
abolished
these
effects.
Mechanistically,
through
RNA
sequencing
(RNA-seq)
ATAC
(ATAC-seq)
analyses,
promotion
by
associated
pathway,
mitochondrial
function,
fatty
acid
metabolism.
These
findings
highlight
regeneration,
which
provides
novel
insights
into
targets
Basic Research in Cardiology,
Journal Year:
2024,
Volume and Issue:
119(3), P. 349 - 369
Published: April 29, 2024
Heart
failure
continues
to
be
a
significant
global
health
concern,
causing
substantial
morbidity
and
mortality.
The
limited
ability
of
the
adult
heart
regenerate
has
posed
challenges
in
finding
effective
treatments
for
cardiac
pathologies.
While
various
medications
surgical
interventions
have
been
used
improve
function,
they
are
not
able
address
extensive
loss
functioning
cardiomyocytes
that
occurs
during
injury.
As
result,
there
is
growing
interest
understanding
how
cell
cycle
regulated
exploring
potential
stimulating
cardiomyocyte
proliferation
as
means
promoting
regeneration.
This
review
aims
provide
an
overview
current
knowledge
on
regulation
mechanisms
underlying
cases
failure,
while
also
highlighting
established
novel
therapeutic
strategies
targeting
this
area
treatment
purposes.
Cell Communication and Signaling,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: June 28, 2024
Abstract
Background
The
neonatal
mammalian
heart
exhibits
considerable
regenerative
potential
following
injury
through
cardiomyocyte
proliferation,
whereas
mature
cardiomyocytes
withdraw
from
the
cell
cycle
and
lose
capacities.
Therefore,
investigating
mechanisms
underlying
proliferation
regeneration
is
crucial
for
unlocking
of
adult
to
repair
damage
restore
contractile
function
myocardial
injury.
Methods
Tudor
staphylococcal
nuclease
(
Tudor-SN
)
transgenic
(TG)
or
cardiomyocyte-specific
knockout
mice
Myh6-Tudor-SN
−/−
were
generated
investigate
role
in
apical
resection
(AR)
surgery.
Primary
isolated
used
assess
influence
on
vitro.
Affinity
purification
mass
spectrometry
employed
elucidate
mechanism.
H9c2
cells
mouse
myocardia
with
either
overexpression
utilized
its
impact
phosphorylation
Yes-associated
protein
(YAP),
both
vitro
vivo.
Results
We
previously
identified
as
a
regulator
that
highly
expressed
but
downregulated
adults.
Our
present
study
demonstrates
sustained
expression
promotes
prolongs
cardiomyocytes,
improves
cardiac
function,
enhances
ability
left
ventricular
apex
mice.
Consistently,
impairs
retards
recovery
after
associates
YAP,
which
plays
important
roles
development
regeneration,
inhibiting
at
Ser
127
397
residues
by
preventing
association
between
Large
Tumor
Suppressor
1
(LATS1)
correspondingly
maintaining
stability
promoting
nuclear
translocation
YAP
enhance
proliferation-related
genes
transcription.
Conclusion
regulates
consequently
enhancing
prolonging
under
physiological
conditions
Graphical
