Retinal Alterations Predict Early Prodromal Signs of Neurodegenerative Disease

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1689 - 1689

Published: Jan. 30, 2024

Neurodegenerative diseases are an increasingly common group of that occur late in life with a significant impact on personal, family, and economic life. Among these, Alzheimer’s disease (AD) Parkinson’s (PD) the major disorders lead to mild severe cognitive physical impairment dementia. Interestingly, those may show onset prodromal symptoms early after middle age. Commonly, evaluation these neurodegenerative is based detection biomarkers, where functional structural magnetic resonance imaging (MRI) have shown central role revealing or phases, although it can be expensive, time-consuming, not always available. The aforementioned visual system due pathophysiological mechanisms shared between eye brain. In disease, α-synuclein deposition retinal cells, as well dopaminergic neurons substantia nigra, alters cortex function, resulting modifications field. Similarly, modified by neurofibrillary tangles neuritic amyloid β plaques typically seen brain, this reflect accumulation biomarkers retina during stages postmortem retinas AD patients. light, ophthalmic neurodegeneration could become cost-effective method for diagnosis diseases, overcoming limitations deep This analysis commonly used practice, interest has risen recent years. review will discuss relationship degeneration, highlighting how represent noninvasive straightforward diseases.

Language: Английский

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Preanalytical Errors in Clinical Laboratory Testing at a Glance: Source and Control Measures

Cureus, Journal Year: 2024, Volume and Issue: unknown

Published: March 30, 2024

The accuracy of diagnostic results in clinical laboratory testing is paramount for informed healthcare decisions and effective patient care. While the focus has traditionally been on analytical phase, attention shifted towards optimizing preanalytical phase due to its significant contribution total errors. This review highlights errors, their sources, control measures improve quality testing. Blood sample a critical concern, with factors such as hemolysis, lipemia, icterus leading erroneous results. Sources errors encompass inappropriate test requests, preparation lapses, during collection, handling, transportation. Mitigating these includes harmonization efforts, education training programs, automated methods assessment, monitoring. Collaboration between personnel professionals crucial implementing sustaining enhance reliability results, ultimately improving

Language: Английский

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Intention-to-diagnose and distinct research foci in diagnostic accuracy studies

The Lancet Infectious Diseases, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

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The interference of baicalein with uric acid detected by the enzymatic method and its correction method

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 10, 2025

In recent years, the frequency of clinical application and international recognition Chinese herbal medicines have been increasing, but effect on common biochemical tests is still unclear. This study aimed to investigate baicalein, a medicine ingredient, uric acid (UA), cholesterol, triglycerides, high-density lipoprotein low-density cholesterol alleviate interference baicalein these assays by improving reagent. The interferences during detection five analytes were investigated Hitachi 7600 system. We prepared UA assay kit according commercial standards facilitate improvement formulation evaluated its performance. Tempol, which could eliminate was found based chemical properties drug, optimum concentration for adding it our reagent determined. that concentration-dependent analytes, with largest negative determination. Self-prepared had safe analysis Our showed higher - 71.75% 89.98% at 200 µg/mL respectively. addition 5 mmol/L Tempol strongly resist baicalein. Conclusion, has Trinder reaction, especially assay. With increase in concentrations, bias increased, improved detection.

Language: Английский

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Alterações laboratoriais dos medicamentos atuantes no sistema nervoso de uma Relação Municipal de Medicamentos Essenciais do nordeste brasileiro

Revista Brasileira de Farmácia Hospitalar e Serviços de Saúde, Journal Year: 2024, Volume and Issue: 15(1), P. 1020 - 1020

Published: March 23, 2024

Objetivo: Realizar um levantamento sobre as interferências nos exames laboratoriais dos medicamentos incluídos na classificação Anatomical Therapeutic Chemical (ATC) N (medicamentos que atuam no sistema nervoso), presentes Relação Municipal de Medicamentos Essenciais (REMUME) do município Fortaleza, estado Ceará. Métodos: Trata-se uma pesquisa exploratória. Utilizou-se como descritores busca os nomes 3º edição REMUME 2022, nas bases dados Bulário Eletrônico da Agência Nacional Vigilância Sanitária e UpToDate®. Em seguida, classificaram-se identificadas em in vitro vivo. Além disso, agruparam-se alterações vivo acordo com a frequência das reações adversas, baseando-se preconizado pelo Medical Dictionary for Regulatory Activities. Os foram compilados analisados usando o software Microsoft Office Excel® 2013. Resultados: Dos 50 analisados, observou-se 16% não apresentaram nenhuma interferência e/ou As classes terapêuticas mais anticonvulsivantes (12%, n=6), seguido antidepressivos, 6% (n=3). No tange às vivo, também classe farmacológica (22%, n=11), seguidos antidepressivos (18%, n=9). Biperideno, clobazam, hidroxizina, nicotina, pramipexol, piridostigmina, rivastigmina rasagilina alteração ou literatura consultada. principais orgânicas envolviam hematológico, destaque para apresentavam desconhecida. Conclusão: Constatamos Fortaleza-CE, utilizados situações clínicas envolvem nervoso central, podem ocasionar laboratoriais. Resultados alterados provocados pelos uso pela doença si, vir comprometer terapêutica paciente, gerando condutas médicas errôneas consequentemente iatrogenias, culminando tempo hospitalização gastos serviços saúde.

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Cherry-Red plasma: Beyond the assumption of hemolysis

Clinica Chimica Acta, Journal Year: 2024, Volume and Issue: 564, P. 119917 - 119917

Published: Aug. 23, 2024

Language: Английский

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Assessing Non-Laboratory Healthcare Professionals’ Attitude towards the Importance of Patient Preparation for Laboratory Tests

Healthcare, Journal Year: 2024, Volume and Issue: 12(10), P. 989 - 989

Published: May 10, 2024

(1) Background: Various guidelines address patient preparation and its importance for venous blood sampling, such as the GP41 guideline issued by Clinical Laboratory Standards Institute (CLSI) collection published World Health Organisation. Recommendations provided national societies or international organisations in field of radiology, The Contrast Media Safety Committee European Society Urogenital Radiology, laboratory medicine, Working Group Preanalytical Phase (WG-PRE) Federation Chemistry Medicine (EFLM) Latin American (WG-PRE-LATAM) Confederation Biochemistry (COLABIOCLI), also guide this practice. There is a notable lack understanding regarding viewpoints held non-laboratory healthcare professionals concerning significance testing impact typical factors associated with preparation. This study endeavours to bridge gap assessing attitude Lithuania these pivotal aspects. (2) Methods: A self-designed anonymous questionnaire was disseminated among 141 public institutions Lithuania. internal consistency evaluated computing Cronbach’s alpha. Descriptive statistics were utilised variables, while comparisons groups conducted using Mann–Whitney U (for two groups) Kruskal–Wallis more than categorical discrete indicators. post-hoc test employed pairwise comparisons. level p-Value < 0.05 applied establish statistical significance. (3) Results: total 158 respondents constituted distinct professionals: nurses physicians. Most participants either agreed strongly that could introduce bias into results. Professionals less 20 years work experience those who attended training sampling within 5-year timeframe exhibited stronger agreement different preanalytical their on results compared counterparts. (4) Conclusions: Non-laboratory participated survey consider proper be significant step towards obtaining accurate They recognize commonly acknowledged important ensuring reliable However, attitudes several vary depending whether have experience, well they any topic last five never training.

Language: Английский

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Drug Interactions and Their Management

Springer eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 77 - 104

Published: Jan. 1, 2024

Language: Английский

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Experimental study of acute toxicity of the intramuscular form of a specific immunobiostimulator — transfer factor

Agrarian science, Journal Year: 2024, Volume and Issue: 1(9), P. 40 - 47

Published: Oct. 11, 2024

Relevance . The article presents data on the toxicological assessment of safety specific immunobiostimulant antigen-directed action “Transfer Factor” in a form ready for intramuscular administration and obtained from blood hyperimmunized donors laboratory animals an acute experiment. Methods object study was finished Factor”, toxicity which determined models (nonlinear mice, nonlinear rats). Results It found that after drug to mice intramuscularly (0.25 ml/head) intraperitoneally (0.5 ml/head), rats (2.5 muscle tremor is observed, disappearing 15–20 minutes. Intraperitoneal dosages 0.75 ml/head 3.75 ml/head, respectively, determines presence lasting 40 During 14-day observation period, body weight increases by 10.15-14.25 11.84-17.67%. Autopsy does not reveal visible changes location organs fluid abdominal pleural cavities. However, some pathological color, consistency size are lungs, heart, spleen liver with Transfer Factor at dose 0.25 2.5 intraperitoneal 0.5 ml/head. Based totality vital signs animals, macroscopic characteristics internal according GOST 12.1.007-76, can be classified as hazard class IV “Low-hazard substances”.

Language: Английский

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Biotin interference with laboratory test results

Hospital Pharmacology - International Multidisciplinary Journal, Journal Year: 2024, Volume and Issue: 11(3), P. 1509 - 1528

Published: Jan. 1, 2024

Introduction: Biotin interference in laboratory tests is a growing concern, particularly due to the widespread use of biotin not only as therapeutic agent but also supplement cosmetic industry. Immunoassays utilizing biotin-streptavidin technology are especially susceptible interference, which can result inaccurate test results, misdiagnosis, and inappropriate treatment. Methodology: This review synthesizes information from scientific literature available through databases like PubMed Google Scholar. Keywords such "biotin", "interference" "immunoassay" were used identify relevant studies. Topic: addresses risk various immunoassays, its impact on thyroid function (TSH, FT3, FT4), parathyroid hormone (PTH), testosterone, human chorionic gonadotropin (hCG), cardiac markers including troponin. It explores pharmacokinetics elimination prevalence elevated levels patient populations. The clinical consequences falsely high or low results , potentially leading highlighted. Methods for mitigating serial dilution, depletion, advancements biotin-resistant discussed. Conclusion: presents significant challenge diagnostics, with high-dose supplements. Tests most include those assessing function, reproductive hormones, markers. Raising awareness among healthcare professionals patients, along implementing depletion protocols improving immunoassay designs, key strategies mitigate interference. Continued research into immunoassays critical enhancing diagnostic accuracy preventing misinterpretation.

Language: Английский

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