Multiple-pulse damage thresholds of retinal explants in the ns-time regime DOI Creative Commons

Scarlett Lipp,

Sebastian Kotzur,

Philipp Elmlinger

et al.

Biomedical Optics Express, Journal Year: 2020, Volume and Issue: 11(12), P. 7299 - 7299

Published: Nov. 13, 2020

The data situation of laser-induced damage measurements after multiple-pulse irradiation in the ns-time regime is limited. Since laser safety standard based on experiments, it crucial to determine thresholds. For a better understanding underlying mechanism repetitive irradiation, we generate thresholds for pulse sequences up N = 20 000 with 1.8 ns-pulses using square-core fiber and pulsed Nd:YAG laser. Porcine retinal pigment epithelial layers were used as tissue samples, irradiated six evaluated by fluorescence microscopy. decreased from 31.16 µJ 1 11.56 000. reduction indicates photo-chemical mechanisms reaching critical energy dose.

Language: Английский

Exploring Retinal Conditions Through Blue Light Reflectance Imaging DOI
Ricardo Luz Leitão Guerra,

Cezar Luz Leitão Guerra,

Mariana Gouveia Bastos Meirelles

et al.

Progress in Retinal and Eye Research, Journal Year: 2025, Volume and Issue: 105, P. 101326 - 101326

Published: Jan. 5, 2025

Language: Английский

Citations

0
Adaptive optics two-photon excited fluorescence lifetime imaging ophthalmoscopy of photoreceptors and retinal pigment epithelium in the living non-human primate eye DOI Creative Commons
Sarah Walters,

James A. Feeks,

Khang T. Huynh

et al.

Biomedical Optics Express, Journal Year: 2021, Volume and Issue: 13(1), P. 389 - 389

Published: Dec. 6, 2021

Fluorescence lifetime imaging has demonstrated promise as a quantitative measure of cell health. Adaptive optics two-photon excited fluorescence (TPEF) ophthalmoscopy enables excitation intrinsic retinal fluorophores involved in cellular metabolism and the visual cycle, providing

Language: Английский

Citations

14
Blue light-induced phototoxicity in retinal cells: implications in age-related macular degeneration DOI Creative Commons
Harshini Chakravarthy,

Vasil Georgyev,

Cole Wagen

et al.

Frontiers in Aging Neuroscience, Journal Year: 2024, Volume and Issue: 16

Published: Dec. 17, 2024

Sunlight exposure is recognized as a risk factor for the development of age-related macular degeneration (AMD), common neurodegenerative retinal disease in elderly. Specifically, blue light wavelengths within sunlight can negatively impact physiology light-sensitive cells, including pigmented epithelium (RPE) and photoreceptors. This review explores light-induced degeneration, emphasizing structural functional impairments RPE. The initial section provides brief overview light's effects on photoreceptors, followed by comprehensive analysis its detrimental

Language: Английский

Citations

1
In Vivo Functional Imaging of Retinal Neurons Using Red and Green Fluorescent Calcium Indicators DOI
Soon Keen Cheong, Wenjun Xiong,

Jennifer M. Strazzeri

et al.

Advances in experimental medicine and biology, Journal Year: 2018, Volume and Issue: unknown, P. 135 - 144

Published: Jan. 1, 2018

Language: Английский

Citations

2
Multiple-pulse damage thresholds of retinal explants in the ns-time regime DOI Creative Commons

Scarlett Lipp,

Sebastian Kotzur,

Philipp Elmlinger

et al.

Biomedical Optics Express, Journal Year: 2020, Volume and Issue: 11(12), P. 7299 - 7299

Published: Nov. 13, 2020

The data situation of laser-induced damage measurements after multiple-pulse irradiation in the ns-time regime is limited. Since laser safety standard based on experiments, it crucial to determine thresholds. For a better understanding underlying mechanism repetitive irradiation, we generate thresholds for pulse sequences up N = 20 000 with 1.8 ns-pulses using square-core fiber and pulsed Nd:YAG laser. Porcine retinal pigment epithelial layers were used as tissue samples, irradiated six evaluated by fluorescence microscopy. decreased from 31.16 µJ 1 11.56 000. reduction indicates photo-chemical mechanisms reaching critical energy dose.

Language: Английский

Citations

1