Ni‐MOF Engineered System Targeting Macrophage Aurora A Kinase for Bone Loss Prevention Through PD‐L1 Activation DOI Open Access

Wenqian Zhang,

Shengming Zhang, Minghao He

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 4, 2024

Abstract Postmenopausal bone loss due to estrogen deficiency necessitates effective therapeutic strategies. Our study explores targeting macrophage Aurora A kinase mitigate loss. phosphorylation is observed being increased in marrow‐derived macrophages (BMDMs) from ovariectomy (OVX) mice, along with a reduction programmed death‐ligand 1 (PD‐L1) expression. Detailed analysis reveals that PD‐L1 plays an immunomodulatory role by lowering the ratio of T helper 17 cells and regulatory cells. The metabolic shift toward glycolysis through transcriptome sequencing, induced inhibition, essential for expression BMDMs. interaction between cytochrome C oxidase subunit 5B found enhance To apply these findings therapeutically, multifunctional system developed using Nickel‐metal organic framework combined bisphosphonate MLN8237 (BP@Ni‐MOF/MLN8237). This targets tissues effectively delivers macrophages, promoting favorable immune environment. Moreover, this exhibits obvious angiogenic effect. present highlights crucial roles maintaining homeostasis as well angiogenesis effect Ni‐MOF engineered system, presenting promising approach prevent postmenopausal

Language: Английский

Revitalizing osteoporotic bone repair via multilevel ROS scavenging and osteoimmune regulating hydrogel DOI
Yiran Zhang, Qingcheng Song, Shuai Yang

et al.

Composites Part B Engineering, Journal Year: 2025, Volume and Issue: unknown, P. 112305 - 112305

Published: Feb. 1, 2025

Language: Английский

Citations

0
Targeted Delivery of α‐ketoglutarate to Macrophages in Bone: A Novel Therapeutic Strategy for Improving Fracture Healing in Type 2 Diabetes DOI Creative Commons
Jing Wang, Jiahao Cao, Siqi Zhang

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Abstract Impaired fracture healing is a common complication in type 2 diabetes mellitus (T2DM), with limited effective treatments. This study investigates the role of macrophages bone repair and introduces novel therapeutic strategy. Reduced glutaminase (GLS) expression glutaminolysis are found from T2DM mice monocytes patients. Specific deletion GLS altered their phenotypes delayed mice. Mechanistically, deficiency reduced α‐ketoglutarate (α‐KG) levels macrophages, which impairs morphogenetic protein (BMP2) production by increasing cytosine methylation on promoter, ultimately hindering osteogenic differentiation marrow mesenchymal stem cells. Importantly, while systemic α‐KG supplementation deteriorates mice, targeted delivery using α‐[email protected]@ALN‐Liposome to markedly improves healing. These findings underscore critical macrophage propose as promising intervention for improving

Language: Английский

Citations

0
Conductive Hydrogel Inspires Neutrophil Extracellular Traps to Combat Bacterial Infections in Wounds DOI Creative Commons

Lizhi Ouyang,

Ze Lin, Xi He

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Thetreatment of infected wounds is currently a major challenge in clinical medicine, and enhancing antimicrobial angiogenic capacity one the most common strategies. However, current treatment makes it difficult to balance effect early stage later stages wound healing, leading an increased rate poor prognosis. Here, we present nanoconductive hydrogel EF@S-HGM, consisting HGM with ECGS, FMLP, SWCNT. The host-guest supramolecular macromolecule (HGM) biocompatible can be injected situ wound. endothelial cell growth factor (ECGS) accelerates vascular remodeling repairs by promoting proliferation cells. N-Formyl-Met-Leu-Phe (FMLP) recruits neutrophils increases capacity. Single-walled carbon nanotubes (SWCNT) make conductive, enabling utilize endogenous electric field recruit multiple kinds In addition, found that EF@S-HGM activates glucocorticoid receptor senescence pathway promotes formation NET, which enhances effect. As tissue-engineered skin, conductive promising material for regenerative medicine may provide potential option care significantly improve patient outcomes

Language: Английский

Citations

0
Matrix Viscoelasticity Orchestrates Osteogenesis via Mechanotransduction Mediated Metabolic Switch in Macrophages DOI Creative Commons

Dihao Tao,

Hanzhe Wang,

Shiping Chang

et al.

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

Understanding the interplay between extracellular matrix (ECM) mechanics and macrophage cellular processes is crucial for bone regeneration. While ECM stiffness has been extensively studied, role of viscoelasticity (e.g., stress relaxation) in marrow niche its effects on function remain unclear. Here, this study reveals how orchestrates osteogenesis by modulating metabolism through vasodilator-stimulated phosphoprotein (VASP) / hypoxia-inducible factor 1 alpha (HIF1α) signaling. In rapid maxillary expansion (RME) model, significant relaxation occurs regenerated during initial 17 days, coinciding with increased transforming growth factor-beta (TGF-β1+) F4/80+ macrophages. Fast enhances recruitment mesenchymal stem cells (MSCs) upregulating TGF-β1. Using a hydrogel-macrophage system mimicking viscoelasticity, cranial defect regeneration significantly improved. Moreover, fast shifts from glycolysis to oxidative phosphorylation (OXPHOS) via VASP/HIF1α signaling, facilitating reparative phenotype. These findings elucidate relationship metabolism, suggesting new therapeutic avenues mechanomedicine.

Language: Английский

Citations

0
Targeting the central and peripheral nervous system to regulate bone homeostasis: mechanisms and potential therapies DOI Creative Commons
Tongzhou Liang, Zheyu Jin, Yuejun Lin

et al.

Military Medical Research, Journal Year: 2025, Volume and Issue: 12(1)

Published: March 20, 2025

Abstract The skeleton is innervated by different types of nerves and receives signaling from the nervous system to maintain homeostasis facilitate regeneration or repair. Although role peripheral signals in regulating bone has been extensively investigated, intimate relationship between central remains less understood, yet it emerged as a hot topic field. In this review, we discussed clinical observations animal studies that elucidate connection metabolism, either intact after injury. First, explored mechanistic linking specific brain nuclei with homeostasis, including ventromedial hypothalamus, arcuate nucleus, paraventricular hypothalamic amygdala, locus coeruleus. We then focused on characteristics innervation nerve subtypes, such sensory, sympathetic, parasympathetic nerves. Moreover, summarized molecular features regulatory functions these Finally, included available translational approaches utilize function improve promote regeneration. Therefore, considering within context neuromusculoskeletal interactions can deepen our understanding skeletal repair process, ultimately benefiting future translation.

Language: Английский

Citations

0
PDGF-BB improves cortical bone quality through restoring the osteogenic microenvironment in the steroid-associated osteonecrosis of rabbits DOI Creative Commons

Huijuan Cao,

Keda Shi,

Jing Long

et al.

Journal of Orthopaedic Translation, Journal Year: 2025, Volume and Issue: 52, P. 97 - 115

Published: April 12, 2025

Language: Английский

Citations

0
Progress of research on the gut microbiome and its metabolite short-chain fatty acids in postmenopausal osteoporosis: a literature review DOI
Qianming Chen, Ying Xie, Xijie Yu

et al.

Frontiers of Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: May 10, 2025

Language: Английский

Citations

0
SRSF1 and SRSF2 synergistically regulate Bim expression to mediate glucocorticoid-induced apoptosis in osteoblasts DOI
Hong Luo, Jian Zhang, Fei Zhang

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2025, Volume and Issue: unknown, P. 152015 - 152015

Published: May 1, 2025

Language: Английский

Citations

0
Knockdown of SMYD3 by RNA Interference Regulates the Expression of Autophagy-Related Proteins and Inhibits Bone Formation in Fluoride-Exposed Osteoblasts DOI Creative Commons
Jie Deng,

Xiaoxiao Zeng,

Kailin Zhang

et al.

Biological Trace Element Research, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 6, 2024

This study aimed to explore the role of histone methyltransferase SET and MYND domain containing 3 (SMYD3) in bone metabolism osteoblasts exposed fluoride. The levels urine fluoride, BALP, OC mRNA expression SMYD3 were determined patients with skeletal fluorosis non-fluoride-exposed people on informed consent. protein, contents, BALP activities detected human osteoblast-like MG63 cells rat primary treated sodium fluoride (NaF) for 48 h. autophagosomes observed by transmission electron microscopy. Then, we knocked down confirm whether it was involved regulation formation related autophagy Wnt/β-catenin pathway. We that significantly increased, while decreased groups. In vitro, activities, many NaF osteoblasts. downregulation inhibited autophagy-related proteins, but no significant changes Our results demonstrated exposure coal-burning pollution caused orthopedic injuries abnormalities hindered normal metabolism. Silencing gene could reduce via inhibiting increase induced

Language: Английский

Citations

1
Reduced graphene oxide loaded with tetrahedral framework nucleic acids for combating orthodontically induced root resorption DOI Creative Commons

Wenxiu Yuan,

Maotuan Huang,

Wenqian Chen

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 13, 2024

Root resorption occurs outside the root or within canal. Regardless of its region, is irreversible and in severe cases, may even cause tooth loss. Clinically, external surface usually a side effect orthodontic movement. However, it frustrating to note that there are almost no effective treatment strategies for orthodontically induced (OIRR) due complexity ambiguity etiology. In current study, we successfully fabricated delivery complex, reduced graphene oxide nanosheet loading with tetrahedral framework nucleic acids (tFNAs-rGO) through self-assembly. No significant cytotoxicity organ-toxicity tFNAs-rGO complex was observed cell counting kit-8 assay (CCK-8) hematoxylin-eosin (HE) staining. Histological staining such as tartrate-resistant acid phosphatase (TRAP) Micro-CT three-dimensional reconstruction were employed explore dynamic changes peri-root tissues OIRR mice. vitro, developed an induction microenvironment testify effects on periodontal ligament cells (PDLCs) macrophages by quantitative RT-PCR, western blot, immunofluorescence The data showed region downregulated osteoclastic activity treatment. Furthermore, our study demonstrated enhanced osteogenic differentiation PDLCs facilitated M2-phenotype polarization ameliorate OIRR. Collectively, insight into nanoscale dual-functional regulating populations remodeling proposes promising therapeutic strategy resorption. An innovative loaded developed. prominently ameliorated (OIRR). exerted dual functionality ameliorating via boosting M2 macrophages. Nanoscale would be potential

Language: Английский

Citations

1