Phenotypic Characteristics of Peripheral Blood Lymphocytes in Patients with Alcohol Dependence in the Dynamics of the Post-Abstinence State DOI Creative Commons
Т.П. Ветлугина, E. Epimakhova, V. D. Prokopieva

et al.

Psikhiatriya, Journal Year: 2025, Volume and Issue: 22(5), P. 49 - 58

Published: Jan. 8, 2025

Background: the damaging effect of ethanol on cells, systems and organs determines relevance studying role immune system in pathogenesis alcohol dependence (alcoholism). The literature reported contradictory data effects cellular immunity that is conditioned by various research techniques, approaches to formation groups, disease stages. Objective: determine phenotypes lymphocytes peripheral blood from patients with time course post withdrawal state. Patients: 52 male aged 30–60 years were examinedwho diagnosed according ICD-10 Mental Behavioral Disorders due Use Alcohol (dependence syndrome — F10.21 F10.30), their duration was 15.0 ± 9.5 years, including 12 alcoholic liver (ALD). investigations conducted post-withdrawal state: after detoxification (1 point) days 14–17 treatment (2 point). 25 conditionally healthy men served as controls, comparison group included 20 neurotic disorders. Methods: populations/subpopulations determined cytometer BD FACS Calibur (Becton Dickinson, USA); reagent kits same firm used. T-lymphocytes (CD3+ ), B-lymphocytes (CD19+ T-helpers/Т-inducers CD4+ cytotoxic Т-lymphocytes CD8+ NK cells (CD3–CD16+ CD56+ ) revealed percent population absolute values. Results: point 1 study, patients, relation controls group, had an elevated Т-helpers-inducers, a reduced number cells. After therapy, CD3+ remain elevated, decrease; (ALD) reduced. Conclusion: phenotype alcohol-dependent at early stage postwithdrawal state characterized elevation , reduction During some populations are normalized, except for T-lymphocytes, well ALD. Immune imbalance indicates instability need additional treatment.

Language: Английский

The Role of Cytokines in the Pathogenesis and Treatment of Alcoholic Liver Disease DOI Creative Commons
Giuseppe Guido Maria Scarlata,

Carmen Colaci,

Marialaura Scarcella

et al.

Diseases, Journal Year: 2024, Volume and Issue: 12(4), P. 69 - 69

Published: March 29, 2024

Alcoholic liver disease (ALD) is a major cause of chronic disease. This term covers broad spectrum lesions, from simple steatosis to alcoholic hepatitis and cirrhosis. The pathogenesis ALD multifactorial not fully elucidated due complex mechanisms related direct ethanol toxicity with subsequent hepatic systemic inflammation. accumulation pro-inflammatory cytokines the reduction anti-inflammatory promote development progression ALD. To date, there are no targeted therapies counter alcohol-related prevent acute failure. Corticosteroids reduce mortality by acting on hepatic-systemic On other hand, several studies analyzed effect inhibiting stimulating as potential therapeutic targets in narrative review aims clarify role main involved treatment

Language: Английский

Citations

4
Macrophages in necrotic liver lesion repair: opportunities for therapeutical applications DOI
Yang Wang, Robim M. Rodrigues, Cheng Chen

et al.

AJP Cell Physiology, Journal Year: 2024, Volume and Issue: 326(5), P. C1556 - C1562

Published: April 15, 2024

Healthy livers contain 80% of body resident macrophages known as Kupffer cells. In diseased livers, the number cells usually drops but is compensated by infiltration monocyte-derived macrophages, some which can differentiate into Kupffer-like Early studies suggest that play important roles in both promoting liver injury and regeneration. Yet, distinction between functionalities infiltrating not always made. By using more specific macrophage markers targeted cell depletion single-cell RNA sequencing, recent revealed several subsets functions inducing damage inflammation well repair this review, we discuss different hepatic necrotic lesion resolution dead clearance, targeting these potential tools for development novel therapies acute failure acute-on-chronic failure.

Language: Английский

Citations

4
New therapeutic target for alcohol-associated hepatitis (AH): AH-associated IL-8+neutrophils DOI Creative Commons
Yukun Guan, Dechun Feng, Luca Maccioni

et al.

eGastroenterology, Journal Year: 2024, Volume and Issue: 2(4), P. e100166 - e100166

Published: Dec. 1, 2024

Language: Английский

Citations

4
Relevance of the GH-VEGFB/VEGFA axis in liver grafts from brain-dead donors with alcohol-associated liver disease DOI Creative Commons
Marc Micó-Carnero, Carlos Rojano-Alfonso,

Cristina Maroto-Serrat

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 7, 2025

Grafts with alcohol-associated liver disease (ALD) subjected to prolonged cold ischaemia from donors after brain death (DBD) are typically unsuitable for transplantation. Here, we investigated the role of growth hormone (GH) in livers ALD DBDs and its relationship vascular endothelial factor A (VEGFA) VEGFB. Livers rats fed ethanol 6 weeks (BD) were stored 24 h ex vivo reperfusion. Hepatic damage proliferative inflammatory parameters analysed BD, before graft retrieval, Survival was monitored using an transplantation model. In DBDs, administration GH, which increased levels intestine but not liver, induced generation both VEGFA VEGFB protected against hepatic caused by BD retrieving grafts donors. However, only that ischemia reperfusion, also survival recipients. conclusion, signalling pathway beneficial properties GH-VEGFA/VEGFB pathway, intestine-liver axis plays a key role, disrupted when retrieved

Language: Английский

Citations

0
Phenotypic Characteristics of Peripheral Blood Lymphocytes in Patients with Alcohol Dependence in the Dynamics of the Post-Abstinence State DOI Creative Commons
Т.П. Ветлугина, E. Epimakhova, V. D. Prokopieva

et al.

Psikhiatriya, Journal Year: 2025, Volume and Issue: 22(5), P. 49 - 58

Published: Jan. 8, 2025

Background: the damaging effect of ethanol on cells, systems and organs determines relevance studying role immune system in pathogenesis alcohol dependence (alcoholism). The literature reported contradictory data effects cellular immunity that is conditioned by various research techniques, approaches to formation groups, disease stages. Objective: determine phenotypes lymphocytes peripheral blood from patients with time course post withdrawal state. Patients: 52 male aged 30–60 years were examinedwho diagnosed according ICD-10 Mental Behavioral Disorders due Use Alcohol (dependence syndrome — F10.21 F10.30), their duration was 15.0 ± 9.5 years, including 12 alcoholic liver (ALD). investigations conducted post-withdrawal state: after detoxification (1 point) days 14–17 treatment (2 point). 25 conditionally healthy men served as controls, comparison group included 20 neurotic disorders. Methods: populations/subpopulations determined cytometer BD FACS Calibur (Becton Dickinson, USA); reagent kits same firm used. T-lymphocytes (CD3+ ), B-lymphocytes (CD19+ T-helpers/Т-inducers CD4+ cytotoxic Т-lymphocytes CD8+ NK cells (CD3–CD16+ CD56+ ) revealed percent population absolute values. Results: point 1 study, patients, relation controls group, had an elevated Т-helpers-inducers, a reduced number cells. After therapy, CD3+ remain elevated, decrease; (ALD) reduced. Conclusion: phenotype alcohol-dependent at early stage postwithdrawal state characterized elevation , reduction During some populations are normalized, except for T-lymphocytes, well ALD. Immune imbalance indicates instability need additional treatment.

Language: Английский

Citations

0