Neuropeptide Dynamics Coordinate Layered Plasticity Mechanisms Adapting Drosophila Circadian Behavior to Changing Environment. DOI Creative Commons
Abhishek Chatterjee,

Joydeep De,

Béatrice Martin

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 18, 2024

Abstract The Drosophila brain contains distinct sets of circadian oscillators responsible for generating the morning and evening bouts locomotor activity, giving rise to a bimodal rest-activity pattern in light-dark cycles. We lack mechanistic understanding how environmental changes reshape this daily profile pattern. Here, we uncover seasonal switch mechanism that remodels bout activity. Under summer-like conditions, an environment favored by fruit flies temperate climates, levels PDF neuropeptide diminish, triggering cascade. Lowered PDFR signaling disinhibits GSK3/SGG advance output. Upon sensing loss, neural activity weakens DN1p-SIF circuit, promoting afternoon rest; leading earlier appearance peak. At same time, functional connections from DN1p LNd strengthen, consequently handing over pacemaker role DN1ps. Taken together, our findings elucidate environment-induced tip balanced output clock network, aligning rhythms with time. Neuropeptide-driven parallel adjustment circuitry protein functioning likely represents conserved strategy across animal species, enabling them adapt their behavior throughout year.

Language: Английский

Molecular mechanisms and physiological importance of circadian rhythms DOI
Alina Patke, Michael W. Young, Sofia Axelrod

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2019, Volume and Issue: 21(2), P. 67 - 84

Published: Nov. 25, 2019

Language: Английский

Citations

989
Light input pathways to the circadian clock of insects with an emphasis on the fruit fly Drosophila melanogaster DOI Creative Commons
Charlotte Helfrich‐Förster

Journal of Comparative Physiology A, Journal Year: 2019, Volume and Issue: 206(2), P. 259 - 272

Published: Nov. 5, 2019

Abstract Light is the most important Zeitgeber for entraining animal activity rhythms to 24-h day. In all animals, eyes are main visual organs that not only responsible motion and colour (image) vision, but also transfer light information circadian clock in brain. The way which entrains appears, however, variable different species. As do vertebrates, insects possess extraretinal photoreceptors addition their (and ocelli) sometimes located close (underneath) eyes, even central These contribute entrainment of clocks degrees. fruit fly Drosophila melanogaster special, because it expresses blue light-sensitive cryptochrome (CRY) directly its neurons, CRY usually regarded as fly’s photoreceptor. Nevertheless, recent studies show retinal almost every neuron similarly rhythm other insects, or more generally spoken animals. Here, I compare input pathways between selected insect species with a focus on Drosophila’s special case.

Language: Английский

Citations

93
Model and Non-model Insects in Chronobiology DOI Creative Commons
Katharina Beer, Charlotte Helfrich‐Förster

Frontiers in Behavioral Neuroscience, Journal Year: 2020, Volume and Issue: 14

Published: Nov. 26, 2020

The fruit fly Drosophila melanogaster is an established model organism in chronobiology, because genetic manipulation and breeding the laboratory are easy. circadian clock neuroanatomy D. one of best-known networks insects basic behavior has been characterized detail this insect. Another chronobiology honey bee Apis mellifera , which diurnal foraging described already early twentieth century. A. hallmarks research on interplay between sociality complex behaviors like sun compass navigation time-place-learning. Nevertheless, there aspects structure function, for example role photoperiodism diapause, can be only insufficiently investigated these two models. Unlike high-latitude flies such as Chymomyza costata or ezoana cosmopolitan do not display a photoperiodic diapause. Similarly, bees go into “real” but most solitary species exhibit obligatory Furthermore, evolved different Hymenoptera independently, wherefore it might misleading to study social Consequently, additional non-model required understand Diptera Hymenoptera. In review, we introduce compare them with other show their advantages limitations general models insect clocks.

Language: Английский

Citations

91
A circadian clock in a nonphotosynthetic prokaryote DOI Creative Commons
Zheng Eelderink-Chen, Jasper Bosman, Francesca Sartor

et al.

Science Advances, Journal Year: 2021, Volume and Issue: 7(2)

Published: Jan. 8, 2021

Bacillus subtilis has a circadian clock, extending the field of chronobiology to nonphotosynthetic bacteria.

Language: Английский

Citations

90
Life at High Latitudes Does Not Require Circadian Behavioral Rhythmicity under Constant Darkness DOI Creative Commons
Enrico Bertolini, Frank K. Schubert, Damiano Zanini

et al.

Current Biology, Journal Year: 2019, Volume and Issue: 29(22), P. 3928 - 3936.e3

Published: Nov. 1, 2019

Language: Английский

Citations

71
Molecular circadian rhythms are robust in marine annelids lacking rhythmic behavior DOI Creative Commons
N. Sören Häfker, Laurenz Holcik,

Audrey M. Mat

et al.

PLoS Biology, Journal Year: 2024, Volume and Issue: 22(4), P. e3002572 - e3002572

Published: April 11, 2024

The circadian clock controls behavior and metabolism in various organisms. However, the exact timing strength of rhythmic phenotypes can vary significantly between individuals same species. This is highly relevant for rhythmically complex marine environments where organismal diversity likely permits occupation different microenvironments. When investigating locomotor Platynereis dumerilii , a model system molecular chronobiology, we found strain-specific, high variability individual worms. patterns were maintained several weeks. A diel head transcriptome comparison behaviorally versus arrhythmic wild-type worms showed that 24-h cycling core transcripts identical both behavioral phenotypes. While similar total number compared to their counterparts, annotation categories transcripts, however, differed substantially. Consistent with phenotype, exhibit an enrichment related neuronal/behavioral processes. In contrast, increased metabolism- physiology-related transcripts. prominent role neuropeptide pigment-dispersing factor (PDF) Drosophila prompted us test possible functional involvement pdf . Differing from its loss impacts overall activity levels but shows only indirect effects on rhythmicity. Our results show given process rhythmicity others. Across population, exist as continuum, no distinct “boundaries” arrhythmicity. We suggest such rhythm breadth important biodiversity resource enabling species quickly adapt heterogeneous or changing environments. times massive sequencing, our work also emphasizes importance time series tests.

Language: Английский

Citations

8
Circadian plasticity evolves through regulatory changes in a neuropeptide gene DOI Creative Commons
Michael P. Shahandeh,

Liliane Abuin,

Lou Lescuyer De Decker

et al.

Nature, Journal Year: 2024, Volume and Issue: 635(8040), P. 951 - 959

Published: Oct. 16, 2024

Many organisms, including cosmopolitan drosophilids, show circadian plasticity, varying their activity with changing dawn–dusk intervals1. How this behaviour evolves is unclear. Here we compare Drosophila melanogaster sechellia, an equatorial, ecological specialist that experiences minimal photoperiod variation, to investigate the mechanistic basis of plasticity evolution2. D. sechellia has lost ability delay its evening peak time under long photoperiods. Screening mutants in melanogaster/D. hybrids identifies a contribution neuropeptide pigment-dispersing factor (Pdf) loss. Pdf exhibits species-specific temporal expression, due part cis-regulatory divergence. RNA interference and rescue experiments using regulatory sequences demonstrate modulation neuropeptide's expression affects degree behavioural plasticity. The region signals selection across populations from different latitudes. We provide evidence confers selective advantage for at elevated latitude, whereas probably suffers fitness costs through reduced copulation success outside range. Our findings highlight gene as hotspot locus evolution might have contributed both melanogaster's global distribution sechellia's specialization. diverged Pdf, conferring

Language: Английский

Citations

6
Selection for reproduction under short photoperiods changes diapause-associated traits and induces widespread genomic divergence DOI Creative Commons
Hannele Kauranen, Johanna Kinnunen, Anna‐Lotta Hiillos

et al.

Journal of Experimental Biology, Journal Year: 2019, Volume and Issue: unknown

Published: Jan. 1, 2019

The incidence of reproductive diapause is a critical aspect life history in overwintering insects from temperate regions. Much has been learned about the timing, physiology and genetics range insects, but how multiple changes involved this other photoperiodically regulated traits are interrelated not well understood. We performed quasinatural selection on reproduction under short photoperiods northern fly species, Drosophila montana, to trace effects photoperiodic by timer / or circadian clock system. Selection changed several associated with diapause, including day length for (CDL), frequency diapausing females that deviate daily 24 h cycles cold tolerance, towards phenotypes typical lower latitudes. However, had no effect period free-running locomotor activity rhythm brain. At genomic level, induced extensive divergence between control line replicates 16 gene clusters signal transduction, membrane properties, immunologlobulins development. These resembled ones detected latitudinally divergent D. montana populations wild SNP genes linked induction. Overall, our study shows affects diapause-associated without disrupting central network generating rhythms locomor activity.

Language: Английский

Citations

38
Flies as models for circadian clock adaptation to environmental challenges DOI Creative Commons
Charlotte Helfrich‐Förster, Enrico Bertolini, Pamela Menegazzi

et al.

European Journal of Neuroscience, Journal Year: 2018, Volume and Issue: 51(1), P. 166 - 181

Published: Sept. 30, 2018

Life on earth is assumed to have developed in tropical regions that are characterized by regular 24 hr cycles irradiance and temperature remain the same throughout seasons. All organisms circadian clocks predict these environmental prepare advance for them. A central question chronobiology how endogenous changed order anticipate very different cyclical conditions such as extremely short long photoperiods existing close poles. Flies of family Drosophilidae can be found all over world-from tropics subarctic regions-making them unprecedented models studying evolutionary processes underlie adaptation latitudes. This review summarizes our current understanding processes. We discuss changes clock genes network brain Drosophilids may caused behavioural adaptations high

Language: Английский

Citations

38
A natural timeless polymorphism allowing circadian clock synchronization in “white nights” DOI Creative Commons
Angélique Lamaze, Chenghao Chen,

Solene Leleux

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: March 31, 2022

Abstract Daily temporal organisation offers a fitness advantage and is determined by an interplay between environmental rhythms circadian clocks. While light:dark cycles robustly synchronise clocks, it not clear how animals experiencing only weak cues deal with this problem. Like humans, Drosophila originate in sub-Saharan Africa spread North up to the polar circle, long summer days or even constant light (LL). LL disrupts clock function, due activation of CRYPTOCHROME, which induces degradation protein TIMELESS (TIM), but temperature are able overcome these deleterious effects LL. We show here that for occur recently evolved natural timeless allele ( ls-tim ) required, encoding less light-sensitive L-TIM addition S-TIM, form encoded ancient s-tim allele. flies can their behaviour semi-natural conditions typical Northern European summers, suggesting functional gain driving Northward spread.

Language: Английский

Citations

17