Comparison of the Therapeutic Efficacy and Autophagy-Mediated Mechanisms of Action of Urine-Derived and Adipose-Derived Stem Cells in Osteoarthritis DOI
Tianhao Xu, Kaibo Zhang, Yunan Hu

et al.

The American Journal of Sports Medicine, Journal Year: 2024, Volume and Issue: 52(12), P. 3130 - 3146

Published: Sept. 23, 2024

Background: Osteoarthritis (OA) is a prevalent and disabling disease that affects significant proportion of the global population. Urine-derived stem cells (USCs) have shown great prospects in treatment OA, but there no study has compared them with traditional cells. Purpose: This aimed to compare therapeutic efficacy mechanisms USCs adipose-derived (ADSCs) for OA treatment. Study Design: Controlled laboratory study. Methods: We biological properties ADSCs using CCK-8, colony formation, EdU, adhesion, apoptosis assays. evaluated protective effects on IL-1β–treated chondrocytes by chemical staining, immunofluorescence, Western blotting. assessed chondrocyte autophagy transmission electron microscopy, also intra-articular injections gross, histological, micro–computed tomography, immunohistochemical analyses an rat model induced anterior cruciate ligament transection. Results: showed higher proliferation, DNA synthesis, anti-apoptotic abilities than ADSCs. Both increased expression cartilage-specific proteins decreased matrix degradation–related inflammatory factors chondrocytes. had greater advantage suppressing MMP-13 enhanced chondrocytes, being more effective The inhibitor 3-MA reduced Conclusion: To our knowledge, this first explore knee Considering superior terms noninvasive acquisition, high cost-benefit ratio, low ethical concerns, suggests they may be promising option under rigorous regulatory pathways. Clinical Relevance: cell source treat strengthens evidence application USCs.

Language: Английский

HWJMSC-EVs promote cartilage regeneration and repair via the ITGB1/TGF-β/Smad2/3 axis mediated by microfractures DOI Creative Commons

Zhian Chen,

Tianhua Zhou, Huan Luo

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: April 12, 2024

Abstract The current first-line treatment for repairing cartilage defects in clinical practice is the creation of microfractures (MF) to stimulate release mesenchymal stem cells (MSCs); however, this method has many limitations. Recent studies have found that MSC-derived extracellular vesicles (MSC-EVs) play an important role tissue regeneration. This study aimed verify whether MSC-EVs promote damage repair mediated by MFs and explore mechanisms. In vitro experiments showed human umbilical cord Wharton’s jelly (hWJMSC-EVs) promoted vitality chondrocytes proliferation differentiation ability bone marrow-derived MSCs. was mainly because hWJMSC-EVs carry integrin beta-1 (ITGB1), MSCs overexpress ITGB1 after absorbing EVs, thereby activating transforming growth factor-β/Smad2/3 axis. a rabbit knee joint model osteochondral defect repair, injection different concentrations into cavity concentration 50 µg/ml significantly improved formation transparent MF surgery. Extraction regenerated revealed changes ITGB1, factor-β, Smad2/3 were directly proportional cartilage. summary, Graphical abstract

Language: Английский

Citations

4
Cathepsin K Inhibitors as Potential Drugs for the Treatment of Osteoarthritis DOI Open Access

Leyre Brizuela,

René Buchet, Carole Bougault

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 2896 - 2896

Published: March 22, 2025

Links between cathepsin K and the pathophysiology of osteoarthritis (OA) can be established, not least because overabundance in serum OA patients upregulation degraded cartilage animal models OA. Chondrocytes, chondroclasts, or osteoclasts contribute to accumulated at diseased osteochondral junction. After a general presentation physiology, as well its degradation processes, we describe function effect on via type II collagen cleavage. An overview most promising inhibitors is then presented, together with their vitro effects. Although intensive research initially focused bone resorption, there growing interest potential these drugs prevent degradation. In this review, summarize pre-clinical clinical trials that support use treatment To date, no molecules are commercially available, although few have undergone trials, but believe development could broaden therapeutic arsenal for

Language: Английский

Citations

0
Mesenchymal stem cell-derived exosomes: an emerging therapeutic strategy for hepatic ischemia-reperfusion injury DOI Creative Commons
Bo Zhao, Jianshu Wei,

Zijian Jiang

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 14, 2025

Hepatic ischemia-reperfusion injury (HIRI) severely threatens the success rates of liver surgery and transplantation. Its complex pathological process involves multiple factors such as oxidative stress, inflammatory responses, ferroptosis, creating an urgent need for new therapeutic strategies. Exosomes derived from mesenchymal stem cells (MSCs) are emerging a next-generation acellular approach. With their outstanding immune-regulatory capabilities, significant reparative functions, good biocompatibility, they leading innovations in field HIRI treatment. This article provides systematic comparison characteristics MSC-derived exosomes(MSC-EXOs) four different sources: adipose tissue, bone marrow, umbilical cord, induced pluripotent cells. Although clinical translation MSC-EXOs still faces challenges variations isolation methods, large-scale production, safety assessments, remarkable effects vast application potential signal arrival era precision treatment HIRI. review not only comprehensive theoretical foundation to promote but also opens up innovative research directions regenerative medicine.

Language: Английский

Citations

0
Comparison of the Therapeutic Efficacy and Autophagy-Mediated Mechanisms of Action of Urine-Derived and Adipose-Derived Stem Cells in Osteoarthritis DOI
Tianhao Xu, Kaibo Zhang, Yunan Hu

et al.

The American Journal of Sports Medicine, Journal Year: 2024, Volume and Issue: 52(12), P. 3130 - 3146

Published: Sept. 23, 2024

Background: Osteoarthritis (OA) is a prevalent and disabling disease that affects significant proportion of the global population. Urine-derived stem cells (USCs) have shown great prospects in treatment OA, but there no study has compared them with traditional cells. Purpose: This aimed to compare therapeutic efficacy mechanisms USCs adipose-derived (ADSCs) for OA treatment. Study Design: Controlled laboratory study. Methods: We biological properties ADSCs using CCK-8, colony formation, EdU, adhesion, apoptosis assays. evaluated protective effects on IL-1β–treated chondrocytes by chemical staining, immunofluorescence, Western blotting. assessed chondrocyte autophagy transmission electron microscopy, also intra-articular injections gross, histological, micro–computed tomography, immunohistochemical analyses an rat model induced anterior cruciate ligament transection. Results: showed higher proliferation, DNA synthesis, anti-apoptotic abilities than ADSCs. Both increased expression cartilage-specific proteins decreased matrix degradation–related inflammatory factors chondrocytes. had greater advantage suppressing MMP-13 enhanced chondrocytes, being more effective The inhibitor 3-MA reduced Conclusion: To our knowledge, this first explore knee Considering superior terms noninvasive acquisition, high cost-benefit ratio, low ethical concerns, suggests they may be promising option under rigorous regulatory pathways. Clinical Relevance: cell source treat strengthens evidence application USCs.

Language: Английский

Citations

1