Chromosome-level genome assemblies of Nicotiana tabacum, Nicotiana sylvestris, and Nicotiana tomentosiformis

Scientific Data, Journal Year: 2024, Volume and Issue: 11(1)

Published: Jan. 26, 2024

Abstract The Solanaceae species Nicotiana tabacum , an economically important crop plant cultivated worldwide, is allotetraploid that appeared about 200,000 years ago as the result of hybridization diploid ancestors sylvestris and tomentosiformis . previously published genome assemblies for these three relied primarily on short-reads, obtained pseudochromosomes only partially covered genomes. In this study, we generated annotated de novo chromosome-level genomes N. which contain 3.99 Gb, 2.32 1.74 respectively sequence data, with 97.6%, 99.5%, 95.9% aligned in chromosomes, represent 99.2%, 98.3%, 98.5% near-universal single-copy orthologs genes. completion levels are comparable to other reference genomes, enabling more efficient synteny-based cross-species research.

Language: Английский

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Genome-wide identification of the N6-methyladenosine regulatory genes reveals NtFIP37B increases drought resistance of tobacco (Nicotiana tabacum L.)

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Feb. 26, 2024

Abstract Background N 6 -methyladenosine (m A) is one of the common internal RNA modifications found in eukaryotes. The m A modification can regulate various biological processes organisms through modulation alternative splicing, polyadenylation, folding, translation, localization, transport, and decay multiple types RNA, without altering nucleotide sequence. three components involved modification, namely writer, eraser, reader, mediate abundance complex collaborative actions. Currently, research on regulatory genes plants still its infancy. Results In this study, we identified 52 candidate tobacco ( Nicotiana tabacum L . ). Gene structure, conserved domains, motif analysis showed structural functional diversity among different subgroups genes. amplification were mainly driven by polyploidization dispersed duplication, duplicated evolved purified selection. Based potential network expression pattern genes, a significant number might play important roles growth, development, stress response processes. Furthermore, have confirmed critical role NtFIP37B , an writer gene tobacco, enhancing drought resistance. Conclusions This study provides useful information for better understanding evolution response, lays foundation further elucidating function tobacco.

Language: Английский

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Probing plant signal processing optogenetically by two channelrhodopsins

Nature, Journal Year: 2024, Volume and Issue: 633(8031), P. 872 - 877

Published: Aug. 28, 2024

Abstract Early plant responses to different stress situations often encompass cytosolic Ca 2+ increases, plasma membrane depolarization and the generation of reactive oxygen species 1–3 . However, mechanisms by which these signalling elements are translated into defined physiological outcomes poorly understood. Here, study basis for encoding specificity in signal processing, we used light-gated ion channels (channelrhodopsins). We developed a genetically engineered channelrhodopsin variant called XXM 2.0 with high conductance that enabled triggering elevations planta. Plant light-induced influx through were studied side effects caused an anion efflux ACR1 4 Although both tools triggered depolarizations, their activation led distinct responses: 2.0-induced signals stimulated production defence mechanisms; 2.0-mediated drought responses. Our findings imply discrete serve as triggers specific metabolic transcriptional reprogramming enabling plants adapt particular situations. optogenetics approach unveiled within leaves, fluxes, accompanied similar electrical signals.

Language: Английский

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O-glycosylation of the transcription factor SPATULA promotes style development in Arabidopsis

Nature Plants, Journal Year: 2024, Volume and Issue: 10(2), P. 283 - 299

Published: Jan. 26, 2024

Abstract O -linked β- N -acetylglucosamine ( -GlcNAc) and -fucose are two sugar-based post-translational modifications whose mechanistic role in plant signalling transcriptional regulation is still largely unknown. Here we investigated how -glycosyltransferase enzymes of Arabidopsis thaliana , SPINDLY (SPY) SECRET AGENT (SEC), promote the activity basic helix–loop–helix transcription factor SPATULA (SPT) during morphogenesis female reproductive organ apex, style. SPY SEC modify amino-terminal residues SPT vivo vitro by attaching -GlcNAc, respectively. This does not impact homo- heterodimerization events, although it enhances affinity for kinase PINOID gene locus its repression. Our findings offer a example effect -GlcNAc on reveal previously unrecognized roles orchestrating style elongation shape.

Language: Английский

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Genome-Wide Identification, Comprehensive Gene Feature, Evolution, and Expression Analysis of Plant Metal Tolerance Proteins in Tobacco Under Heavy Metal Toxicity

Frontiers in Genetics, Journal Year: 2019, Volume and Issue: 10

Published: April 24, 2019

Plant metal tolerance proteins (MTPs) comprise a family of membrane divalent cation transporters that play essential roles in plant mineral nutrition maintenance and heavy stresses resistance. However, the evolutionary relationships biological functions MTP tobacco remain unclear. In present study, 26, 13 12 MTPs three main Nicotiana species (N. tabacum, N.sylvestris N. tomentosiformis) were identified, respectively, designated based on orthologous relationship to Arabiodopsis MTPs. The phylogenetic relationships, gene structures, chromosome distributions, conserved motifs domains NtMTPs systematic analyzed. According features, 26 classified into major substrate-specific groups (Zn-CDFs, Zn/Fe-CDFs Mn-CDFs) seven primary (1, 5, 6, 7, 8, 9 12). All contained modified signature sequence cation_efflux domain, whereas some them also harbored ZT_dimer. Evolutionary analysis showed NtMTP N.tabacum originated from its parental genome sylvestris tomentosiformis, further underwent lost expanded via one segmental duplication event. Moreover, prediction cis-acting elements microRNA target sites genes suggested diverse complex regulatory mechanisms control expression. Expression profile derived transcriptome data quantitative real-time PCR (qRT-PCR) tissue expression patterns same group similar but varied among groups, indicating growth development. addition, under toxicity, exhibited various responses either leaves or roots. 20 found response at least ion treatment roots, respectively. These results provided perspective evolution helpful for functional characterization genes.

Language: Английский

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