Chromosomal-level Genome Assembly of the Coffee Bee Hawk Moth Reveals the Evolution of Chromosomes and the Molecular Basis of Distinct Phenotypes DOI Creative Commons
Takahiro Yamabe, Rei Kajitani, Atsushi Toyoda

et al.

Genome Biology and Evolution, Journal Year: 2023, Volume and Issue: 15(9)

Published: July 25, 2023

Cephonodes hylas, the coffee bee hawk moth is a species with unique characteristics, such as larvae feeding on gardenia, overcoming toxicity of its iridoid glycosides, diurnal adults, and transparent wings. Although C. hylas fascinating model for molecular biological research, genome sequence analysis-based genetic approaches to elucidate these peculiarities have not yet been undertaken. We successfully achieved de novo assembly at chromosome level comparable Lepidoptera organism, silkworm. Additionally, 16,854 protein-coding genes were annotated, constructed annotated highest quality BUSCO completion compared closely related species. Comparative analysis revealed process chromosomal evolution from Bombycoidea ancestral (n = 31) changes in turnover associated fusion events, rate repetitive insertion. These analyses only possible because was level. increased nonsynonymous/synonymous (dN/dS) ratios observed multiple photoreceptor-related that strongly acquisition activity. Furthermore, tandemly duplicated expanded containing many digestive other enzymes larval midgut-specific expression also confirmed. may be involved metabolism genipin, toxin found gardenias. Using determined level, we identified new insights into Bombycoidea, well relationship between characteristic traits.

Language: Английский

Long read genome assembly of Automeris io (Lepidoptera: Saturniidae) an emerging model for the evolution of deimatic displays DOI Creative Commons
Chelsea Skojec, R. Keating Godfrey, Akito Y. Kawahara

et al.

G3 Genes Genomes Genetics, Journal Year: 2024, Volume and Issue: 14(3)

Published: Feb. 7, 2024

Automeris moths are a morphologically diverse group with 135 described species that have geographic range spans from the New World temperate zone to Neotropics. Many elaborate hindwing eyespots thought deter or disrupt attack of potential predators, allowing moth time escape. The Io (Automeris io), known for its striking eyespots, is well-studied within genus and an emerging model system study evolution deimatism. Existing research on eyespot pattern development will be augmented by genomic resources allow experimental manipulation this model. Here, we present high-quality, PacBio HiFi genome assembly aid existing molecular future other deimatic traits. This 490 Mb highly contiguous (N50 = 15.78 mbs) complete (benchmarking universal single-copy orthologs 98.4%). Additionally, were able recover genes previously identified as being involved in wing formation movement.

Language: Английский

Citations

4
Cytogenetics of insects in the era of chromosome-level genome assemblies DOI Creative Commons
Vladimir A. Lukhtanov, Elena A. Pazhenkova

Vavilov Journal of Genetics and Breeding, Journal Year: 2025, Volume and Issue: 29(2), P. 230 - 237

Published: April 10, 2025

Over the past few years, a revolution has occurred in cytogenetics, driven by emergence and spread of methods for obtaining high-quality chromosome-level genome assemblies. In fact, this led to new tool studying chromosomes chromosomal rearrangements, is thousands times more powerful than light microscopy. This revolutionized cytogenetics many groups insects which previously karyotype information, if available at all, was limited chromosome number. Even impressive are achievements genomic approach general patterns organization evolution insects. Thus, it been shown that rapid transformations numbers, often found order Lepidoptera, most carried out parsimonious way, as result simple fusions fissions chromosomes. It established these not random occur independently different phylogenetic lineages due reuse same ancestral breakpoints. tendency correlated with presence so-called interstitial telomeres, i.e. telomere-like structures located ends chromosomes, but inside them. revealed that, insects, telomeric DNA just set short repeats, very long sequence consisting (TTAGG) n (or other motifs), regularly specifically interrupted retrotransposons, motifs diverse terms their length nucleotide composition. The number assemblies GenBank database growing exponentially now exceeds thousand species. Therefore, exceptional prospects using data analysis beyond doubt.

Language: Английский

Citations

0
Chromosome-level genome assembly and annotation of Clanis bilineata tsingtauica Mell (Lepidoptera: Sphingidae) DOI Creative Commons

Yulu Yan,

Ke Zhao,

Longwei Yang

et al.

Scientific Data, Journal Year: 2024, Volume and Issue: 11(1)

Published: Sept. 30, 2024

Language: Английский

Citations

1
A high-quality, long-read genome assembly of the whitelined sphinx moth (Lepidoptera: Sphingidae: Hyles lineata) shows highly conserved melanin synthesis pathway genes DOI Creative Commons
R. Keating Godfrey, Sarah Britton,

Shova Mishra

et al.

G3 Genes Genomes Genetics, Journal Year: 2023, Volume and Issue: 13(6)

Published: April 29, 2023

Abstract The sphinx moth genus Hyles comprises 29 described species inhabiting all continents except Antarctica. diverged relatively recently (40–25 MYA), arising in the Americas and rapidly establishing a cosmopolitan distribution. whitelined moth, lineata, represents oldest extant lineage of this group is one most widespread abundant moths North America. lineata exhibits large body size adept flight control characteristic family (Sphingidae), but it unique displaying extreme larval color variation broad host plant use. These traits, combination with its distribution high relative abundance within range, have made H. model organism for studying phenotypic plasticity, plant–herbivore interactions, physiological ecology, control. Despite being well-studied moths, little data exist on genetic or regulation gene expression. Here, we report high-quality genome showing contiguity (N50 14.2 Mb) completeness (98.2% Lepidoptera BUSCO genes), an important first characterization to facilitate such studies. We also annotate core melanin synthesis pathway genes confirm that they sequence conservation other are similar those another, well-characterized tobacco hornworm (Manduca sexta).

Language: Английский

Citations

3
Chromosomal-level Genome Assembly of the Coffee Bee Hawk Moth Reveals the Evolution of Chromosomes and the Molecular Basis of Distinct Phenotypes DOI Creative Commons
Takahiro Yamabe, Rei Kajitani, Atsushi Toyoda

et al.

Genome Biology and Evolution, Journal Year: 2023, Volume and Issue: 15(9)

Published: July 25, 2023

Cephonodes hylas, the coffee bee hawk moth is a species with unique characteristics, such as larvae feeding on gardenia, overcoming toxicity of its iridoid glycosides, diurnal adults, and transparent wings. Although C. hylas fascinating model for molecular biological research, genome sequence analysis-based genetic approaches to elucidate these peculiarities have not yet been undertaken. We successfully achieved de novo assembly at chromosome level comparable Lepidoptera organism, silkworm. Additionally, 16,854 protein-coding genes were annotated, constructed annotated highest quality BUSCO completion compared closely related species. Comparative analysis revealed process chromosomal evolution from Bombycoidea ancestral (n = 31) changes in turnover associated fusion events, rate repetitive insertion. These analyses only possible because was level. increased nonsynonymous/synonymous (dN/dS) ratios observed multiple photoreceptor-related that strongly acquisition activity. Furthermore, tandemly duplicated expanded containing many digestive other enzymes larval midgut-specific expression also confirmed. may be involved metabolism genipin, toxin found gardenias. Using determined level, we identified new insights into Bombycoidea, well relationship between characteristic traits.

Language: Английский

Citations

1