Functional and evolutionary comparative analysis of the DIR gene family in Nicotiana tabacum L. and Solanum tuberosum L. DOI Creative Commons
Tong Li,

Wenbin Luo,

Chaofan Du

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: July 5, 2024

Abstract Background The dirigent ( DIR ) genes encode proteins that act as crucial regulators of plant lignin biosynthesis. In Solanaceae species, members the gene family are intricately related to growth and development, playing a key role in responding various biotic abiotic stresses. It will be great application significance analyze expression profile under pathogen stresses species. Results A total 57 tobacco NtDIRs 33 potato StDIRs were identified based on their respective genome sequences. Phylogenetic analysis tobacco, potato, eggplant Arabidopsis thaliana revealed three distinct subgroups (DIR-a, DIR-b/d DIR-e). Gene structure conserved motif showed high degree conservation both exon/intron organization protein motifs among genes, especially within same subfamily. Total 8 pairs tandem duplication (3 5 potato) 13 segmental (6 7 events. Cis -regulatory elements promoters participated hormone response, stress responses, circadian control, endosperm expression, meristem expression. Transcriptomic data diverse response patterns pathogens, indicating functional divergence. After 96 h post-inoculation with Ralstonia solanacearum L. Ras ), seedlings exhibited typical symptoms bacterial wilt. qRT-PCR 11 selected NtDIR displayed differential pattern infection. Using line 392278 material, late blight manifested seedling leaves Phytophthora infestans StDIR up-regulation Notably, clustered NtDIR2 , NtDIR4 StDIR3 robust infection, highlighting essential roles disease resistance. Conclusion genome-wide identification, evolutionary analysis, profiling infection have provided valuable insights into these conditions. Our results could provide basis for further

Language: Английский

Genome-wide identification and expression analysis of the ADH gene family in Artemisia annua L. under UV-B stress DOI Creative Commons
Hengyu Pan, Peiqi Shi, Shan Zhong

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 19, 2025

ADHs are key genes that catalyze the interconversion between alcohols and aldehydes, which play crucial roles in plant adaptation to a range of abiotic stresses. However, characterization evolutionary pathways ADH antimalarial Artemisia annua still unclear. This study identified 49 A. conducted detailed analysis their structural features, conserved motifs, duplication types, revealing tandem dispersed duplications primary mechanisms gene expansion. Evolutionary ( AanADH ) argyi AarADH revealed dynamic changes, with 35 deriving from most recent common ancestor both species. ADH1 , for artemisinin production, had two copies species, expanding via but whole-genome . CREs WGCNA suggested may be regulated by UV-B stress. Following short-term treatment, 16 DEGs were identified, including AanADH6 AanADH7 ), these significantly downregulated after hours treatment (UV2h) upregulated four (UV4h). The expression changes further confirmed GO enrichment qRT-PCR experiments. Overall, this comprehensively characterized family systematically responsive stress, providing foundation research on stress responses.

Language: Английский

Citations

0
Transcriptome analysis reveals the role of microbial volatile 3-Methyl-1-butanol-induced salt stress tolerance in rice (Oryza sativa L.) seedlings through antioxidant defense system DOI

Diem-Kieu Nguyen,

Tri-Phuong Nguyen, Chih-Cheng Lin

et al.

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 109830 - 109830

Published: March 1, 2025

Language: Английский

Citations

0
Functional and evolutionary comparative analysis of the DIR gene family in Nicotiana tabacum L. and Solanum tuberosum L. DOI Creative Commons
Tong Li,

Wenbin Luo,

Chaofan Du

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: July 5, 2024

Abstract Background The dirigent ( DIR ) genes encode proteins that act as crucial regulators of plant lignin biosynthesis. In Solanaceae species, members the gene family are intricately related to growth and development, playing a key role in responding various biotic abiotic stresses. It will be great application significance analyze expression profile under pathogen stresses species. Results A total 57 tobacco NtDIRs 33 potato StDIRs were identified based on their respective genome sequences. Phylogenetic analysis tobacco, potato, eggplant Arabidopsis thaliana revealed three distinct subgroups (DIR-a, DIR-b/d DIR-e). Gene structure conserved motif showed high degree conservation both exon/intron organization protein motifs among genes, especially within same subfamily. Total 8 pairs tandem duplication (3 5 potato) 13 segmental (6 7 events. Cis -regulatory elements promoters participated hormone response, stress responses, circadian control, endosperm expression, meristem expression. Transcriptomic data diverse response patterns pathogens, indicating functional divergence. After 96 h post-inoculation with Ralstonia solanacearum L. Ras ), seedlings exhibited typical symptoms bacterial wilt. qRT-PCR 11 selected NtDIR displayed differential pattern infection. Using line 392278 material, late blight manifested seedling leaves Phytophthora infestans StDIR up-regulation Notably, clustered NtDIR2 , NtDIR4 StDIR3 robust infection, highlighting essential roles disease resistance. Conclusion genome-wide identification, evolutionary analysis, profiling infection have provided valuable insights into these conditions. Our results could provide basis for further

Language: Английский

Citations

3