Coral microbiome manipulation elicits metabolic and genetic restructuring to mitigate heat stress and evade mortality

Science Advances, Journal Year: 2021, Volume and Issue: 7(33)

Published: Aug. 13, 2021

Probiotics mitigate post-heat stress disorder, preventing coral mortality.

Language: Английский

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Harnessing the microbiome to prevent global biodiversity loss

Nature Microbiology, Journal Year: 2022, Volume and Issue: 7(11), P. 1726 - 1735

Published: July 21, 2022

Language: Английский

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Chemical and genomic characterization of a potential probiotic treatment for stony coral tissue loss disease

Communications Biology, Journal Year: 2023, Volume and Issue: 6(1)

Published: April 6, 2023

Abstract Considered one of the most devastating coral disease outbreaks in history, stony tissue loss (SCTLD) is currently spreading throughout Florida’s reefs and greater Caribbean. SCTLD affects at least two dozen different species has been implicated extensive losses cover. Here we show Pseudoalteromonas sp. strain McH1-7 broad-spectrum antibacterial activity against SCTLD-associated bacterial isolates. Chemical analyses indicated produces potential antibacterials, korormicin tetrabromopyrrole, while genomic analysis identified genes potentially encoding an L-amino acid oxidase multiple metalloproteases (pseudoalterins). During laboratory trials, arrested or slowed progression on 68.2% diseased Montastraea cavernosa fragments treated ( n = 22), it prevented transmission by 100% 12). chemically characterized probiotic that effective prophylactic direct treatment for destructive as well a alternative to antibiotic use.

Language: Английский

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Probiotics reshape the coral microbiome in situ without detectable off-target effects in the surrounding environment

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: April 9, 2024

Beneficial microorganisms for corals (BMCs), or probiotics, can enhance coral resilience against stressors in laboratory trials. However, the ability of probiotics to restructure microbiome situ is yet be determined. As a first step elucidate this, we inoculated putative probiotic bacteria (pBMCs) on healthy colonies Pocillopora verrucosa Red Sea, three times per week, during 3 months. pBMCs significantly influenced microbiome, while surrounding seawater and sediment remained unchanged. The genera Halomonas, Pseudoalteromonas, Bacillus were enriched probiotic-treated corals. Furthermore, treatment also correlated with an increase other beneficial groups (e.g., Ruegeria Limosilactobacillus), decrease potential pathogens, such as Vibrio. all (treated non-treated) throughout experiment, could not track health improvements protection stress. Our data indicate that healthy, therefore stable, microbiomes restructured situ, although repeated continuous inoculations may required these cases. Further, our study provides supporting evidence that, at studied scale, have no detectable off-target effects near

Language: Английский

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Microbial-Based Therapies to Restore and Rehabilitate Disrupted Coral Health

Coral reefs of the world, Journal Year: 2025, Volume and Issue: unknown, P. 181 - 195

Published: Jan. 1, 2025

Language: Английский

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Coral restoration and adaptation in Australia: The first five years

PLoS ONE, Journal Year: 2022, Volume and Issue: 17(11), P. e0273325 - e0273325

Published: Nov. 30, 2022

While coral reefs in Australia have historically been a showcase of conventional management informed by research, recent declines cover triggered efforts to innovate and integrate intervention restoration actions into frameworks. Here we outline the multi-faceted approaches that developed since 2017, from newly implemented in-water programs, research enhance resilience investigations socio-economic perspectives on goals. We describe projects using gardening, substrate stabilisation, repositioning, macro-algae removal, larval-based techniques. Three areas focus are also presented illustrate breadth Australian restoration, (1) transdisciplinary Reef Restoration Adaptation Program (RRAP), one world’s largest development programs focused reefs, (2) interventions performance under climate change, (3) socio-cultural perspectives. Together, these reflect an increasing urgency for action confront reef crisis, develop new additional tools manage consequent increase funding opportunities appetite implementation. The rapid progress trialling deploying builds decades overseas experience, advances showing positive signs can be valuable tool improve at local scales (i.e., high early survival rates across variety methods species, strong community engagement with stakeholders). RRAP is creating help multiple scales, micro targeting small within specific site) large core ecosystem function social-economic values select sites Great Barrier Reef) resist, adapt recover impacts change. None aim single-handedly restore entirety Reef, nor do they negate importance urgent change mitigation action.

Language: Английский

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Horizon scanning the application of probiotics for wildlife

Trends in Microbiology, Journal Year: 2023, Volume and Issue: 32(3), P. 252 - 269

Published: Sept. 25, 2023

The provision of probiotics benefits the health a wide range organisms, from humans to animals and plants. Probiotics can enhance stress resilience endangered many which are critically threatened by anthropogenic impacts. use so-called 'probiotics for wildlife' is nascent application, field needs reflect on standards its development, testing, validation, risk assessment, deployment. Here, we identify main challenges this emerging intervention provide roadmap validate effectiveness wildlife probiotics. We cover essential inert negative controls in trials investigation probiotic mechanisms action. also suggest alternative microbial therapies that could be tested parallel with application. Our recommendations align approaches used humans, aquaculture, plants concept wildlife.

Language: Английский

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Mitigating the ecological collapse of coral reef ecosystems

EMBO Reports, Journal Year: 2023, Volume and Issue: 24(4)

Published: March 2, 2023

Science & Society2 March 2023Open Access Transparent process Mitigating the ecological collapse of coral reef ecosystems Effective strategies to preserve Christian R Voolstra Corresponding Author [email protected] orcid.org/0000-0003-4555-3795 Department Biology, University Konstanz, Germany Contribution: Conceptualization, Writing - original draft, review editing Search for more papers by this author Raquel S Peixoto orcid.org/0000-0002-9536-3132 Red Sea Research Center, King Abdullah and Technology (KAUST), Thuwal, Saudi Arabia Christine Ferrier-Pagès orcid.org/0000-0002-0357-4486 Coral Ecophysiology Team, Centre Scientifique de Monaco, Monte Carlo, Monaco Information *,1, *,2 *,3 1Department 2Red 3Coral *Corresponding author. E-mail: EMBO Reports (2023)24:e56826https://doi.org/10.15252/embr.202356826 PDFDownload PDF article text main figures. Peer ReviewDownload a summary editorial decision including letters, reviewer comments responses feedback. ToolsAdd favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures Info are biodiversity hotspots that provide habitat about third all marine species (Fisher et al, 2015)—which is why colloquially they referred as "rainforests sea". In addition their immense importance, reefs offer wealth ecosystem services millions people, provision food commercial fisheries, tourism, sand production, carbon sequestration, coastal protection from storms (Eddy 2021). The crucial organisms establish expand corals, sessile animals build impressive three-dimensional structures through calcium carbonate skeletons, rivaling busy cityscapes. … holobionts fragile threatened local global stressors point where very existence globally now at stake. But corals cannot achieve these constructions alone. Rather, have rely on multitude little helpers. fact, so-called or metaorganisms encompass myriad associated symbiotic microorganisms, collectively microbiome includes archaea, bacteria, fungi, viruses, microeukaryotes, most importantly, Symbiodiniaceae (LaJeunesse 2018; These dinoflagellate photosynthetic microalgae live inside cells them with energy construct skeletons. Despite massive lasting create, stake (Allen 2018). Climate change, owing increasing greenhouse gas (GHG) emissions caused human activities, greatest threat reefs. GHG change conditions in several ways, ocean warming, acidification, an increased frequency intensity tropical heatwaves Frölicher While can locally devastate seawater acidification reduces calcification rates taxa thus skeletal growth (Mollica 2018), warmer waters pose significant (Kleypas 2021; Knowlton Extended periods high temperature cause heat stress, which triggers breakdown symbiosis between Symbiodiniaceae, phenomenon known bleaching (Suggett Smith, 2020). Mass has been over past decade(s) 30% decline population (Eakin 2022). Recent estimations predict that, if warming exceeds 1.5°C, 70–90% risk be lost, 99% will lost 2°C above pre-industrial temperatures (Hoegh-Guldberg actions save connected each other 'and' not 'or'. effects climate amplified stressors, such pollution, sedimentation, eutrophication, land clearing fertilizer use (Wiedenmann 2012). latter causes overgrowth macroalgae bioerosion algal skeletons endolithic algae. It affects microbiome, instance, abundance pathogens (Leite Taken together, driven 2022) along reduce important reef-forming taxa, decrease accretion dissolution sediments (Eyre further weaken stress resilience (Donovan 2021) (Fig 1). Figure 1.(A) A healthy moderate level bleaching. bleached colonies appear white recover stressful subside. (B) degraded dead remaining skeleton overgrown Some visible lower middle. Download figure PowerPoint CO2 emission mitigation pre-requisite follows must protected order 2). International Reef Society (ICRS) proposed three equally pillars saving restoring (Knowlton first one mitigating threats. Importantly, options premise we becoming neutral due time; words, "and" "or" 2.Global pressures led loss cover (left). (right): (A) emissions; mitigate (e.g., managing fish stocks improving water quality); (C) active restoration/rehabilitation. note without reducing curb below eventually neutral, still lose majority (C, right-hand side). interventions occur large areas effective should reinforced socio-economic incentives regulatory measures. necessary limit mean increase threshold trajectory become permanently Staying allow us protect resilient restore damaged Other sea surface (SST), pumping deep cool into modifying solar radiation—through shading, albedo enhancement, stratospheric aerosol injection, so on—represent geoengineering approaches offset impacts (National Academies Sciences, Engineering, Medicine, 2019). expensive best considered only small scale exhibit thermal deserve special protection, because communities evolved natural higher tolerance… Saving conservation Failure address undermine attempts threats, second pillar ICRS's guideline synergistically interact affect Although some under than 1.5°C present but slow, model estimates indicate combination reduced improved conditions, quality, maintain positive budget, is, (Kennedy 2013). Improving requires variety directly indirectly health recovery, reduction overfishing establishment complete partial (MPAs) and/or management zones watersheds nutrient loading river runoff (Mellin 2016). There Caribbean, Australia, Kenya, demonstrate impact recovery However, also adapted particular threats location (Voolstra For example, Gulf Aqaba (GoA) northern coined refuge SST rise withstand up +6°C maximum summer ex situ no mass observed (Osman Yet, immune other, are, indeed, affected antiscalants desalination plants, light pollution. Long-term monitoring national programs, science-guided management, engagement policymakers, well support communities, essential identify appropriate manage conditions. restoration rehabilitation anthropogenic being addressed, put forward ICRS, 2021), development implementation Given pace severity current impacts, efforts mandatory step while achieving neutrality Such customized target different entities holobiont, symbionts, prokaryotic community, microeukaryotes 2019; combine (van Oppen 2015; Boström-Einarsson 2020; Santoro Different levels degradation require (Peixoto Fig 3). considering stage ongoing widespread modern necessarily need integrate prevention concepts succeed, two terms therefore used interchangeably (Box 1) 3.Examples restore/rehabilitate loss. Restoration refers processes help ecosystems; improve adaptive capacity resilience. Many go hand-in-hand many entail component often interchangeably. Box 1. versus rehabilitation. definition "restoration" "Society Ecological Restoration" "the assisting degraded, damaged, destroyed". goals include re-establishment pre-existing composition community structure, environmental faced results future harboring compositions This recognized UNEP's guide restoration, term "coral restoration" describe measures "aim assist function, key face rising pressures, promoting sustainable delivery services". By comparison, "rehabilitation" centered notion "future-proof" reefs, it sufficient merely composition, enhance interventions, probiotic provision, hardening, similar measures, promote extend adaptation, Thus, throughout document consistency, accurately understood form At scale, counter particularly physically storms, disease outbreaks, bleaching, activities. useful option regrowth recruitment limited disturbances reduced. commonly methods involve removal predators reintroduction control macroalgal overgrowth, transplantation fragments intervening nursery phase (Boström-Einarsson One difference sexually asexually propagated addresses genetic diversity deployment artificial aggregation, substrate manipulations, release larvae after intermediate rearing selected measure(s) informed specific engage communities. tolerance, constituting "super reefs" (https://superreefs.whoi.edu), "priority (https://www.50reefs.org), "bright spots" (Cinner consideration potential constitute oases" (Guest turbid near mangroves, latitude upwelling areas, nutrient-rich sheltered waves. extent transferred debatable, particular, reside marginal environments featuring unique adjustments either when transplanted common environments. Consequently, long-term buffering against multiple rarely found. GoA corals—and "bleaching resistant" reefs—that exceptionally (Savary may exposed (local) pollution large, our understanding what underlies various costs trade-offs (Cornwell reintroducing enhancing biomass management/prevention adapt changes. laboratory experiments shown feeding planktonic prey significantly increases resistance (Grottoli 2006). observations found correlation patterns availability populations around world, suggesting phyto- zooplankton concentrations better able disturbance. heterotrophic diet provides macronutrients metals sustain photosynthesis enhances translocation symbionts host (Ferrier-Pagès Increasing nutritional quality plankton provided corals—by manipulating content fatty acids, metals, antioxidant compounds—might strategy health. knowledge, studies attempted alter during waves, partially broad measure biota unknown consequences. total, US National Science, Medicine lists 23 types assisted gene flow (AGF), evolution, colonization, cryopreservation, manipulation AGF aim genotypes within existing optimally suited (Humanes 2022), fitness distant introducing respective alleles populations. Corals survived waves those Persian/Arabian (PAG), highest world occur, good candidates exploring mechanisms means AGF. PAG heat-specialized endosymbiont, Cladocopium thermophilum (Hume 2016), expression heat-responsive genes. Assisted colonization stress-resistant variants AGF, although factors considered, coming back above-mentioned trade-offs. As such, material reproduced generate novel allele combinations convey harbor compatibility prevailing More sophisticated breeding genetically-modified organisms, new traits do exist created resilience… Nurseries accelerate out-planting produced crosses. cryopreserved sperm produce offspring, especially endangered (Hagedorn 2017). biggest challenges scaling smaller, laboratory-sized high-throughput reproduction. genetically modified 2015). To end, available, repeated exposure transgenerational acclimation epigenetic mechanisms, controversy remains (Torda Another approach discussed induction mutagenesis resistant strains, fidelity host–symbiont associations needs addressed Howells work (Buerger restructuring microeukaryotic probiotics (Ziegler 2017; 2019, Zhang exact underlying unclear, seem pathogen toxic compound among others. Continuous insult effectively alters beneficial pathogenic assemblage well-being. rather biomass, could focus extant Microbiome-based customizable applied preventive remediation 2017) holobiont (Zhang (Rosado 2019), oil (Silva thermally prevent mortality (Santoro challenge evaluate efficiency stewardship develop ways applications Securing "Modern" existed ~ 250 million years highly adaptable. study conducted Oculina patagonica showed severe losing tissues remain alive (Fine Tchernov, 2007); never really disappear, even do. long there hope cost effort perspective, enough repopulate once reached stabilized. pristine longer exist, pressure: tolerant recent repeat events, evidence certain clearly likely survive survival comes expense biodiversity, same past. emphases placed securing functions services. Under constraint, recognize ability consider prioritizing chance promotes regeneration through, larval dispersal. local, regional, scales propagation evolutionary (Colton achieved coordinated action science, policy, stakeholders Kleypas strategies, policies, outlined places, depending financial resources, socioeconomic circumstances. We triad emissions, undertaking restoration/rehabilitation, closing window opportunity secure ecosystems—for generations. Acknowledgement Open funding enabled organized Projekt DEAL. contributions Voolstra: Conceptualization; writing—original draft; writing—review editing. Peixoto: Ferrier-Pagès: Disclosure competing interests statement authors declare conflict interest. Supporting References Allen MR, Dube OP, Solecki W, Aragon-Durand F, Cramer Humphreys S, Zickfeld K (2018) Framing Context "in Global Warming 1.5°C: An IPCC Special Report related pathways, context strengthening response development, eradicate poverty.Google Scholar L, Babcock RC, Bayraktarov E, Ceccarelli D, Cook N, Ferse SCA, Hancock B, Harrison P, Hein M, Shaver E al (2020) systematic methods, successes, failures directions. PLoS 15: e0226631CrossrefCASPubMedWeb Science®Google Buerger Alvarez-Roa C, Coppin CW, Pearce SL, Chakravarti LJ, Oakeshott JG, Edwards OR, Van MJH Heat-evolved microalgal tolerance. Sci Adv 6: eaba2498CrossrefCASPubMedWeb Cinner JE, Huchery MacNeil MA, Graham NA, McClanahan TR, Maina J, Maire Kittinger JN, Hicks CC, Mora C (2016) Bright spots world's Nature 535: 416–419CrossrefCASPubMedWeb Colton McManus LC, Schindler DE, Mumby PJ, Palumbi SR, Webster MM, Essington TE, Fox HE, Forrest DL, Schill SR (2022) harness adaptation. Ecol Evol 1405–1407CrossrefPubMedWeb Cornwell Armstrong K, Walker NS, Lippert Nestor V, Golbuu Y, (2021) Widespread variation tolerance symbiont load tradeoffs Acropora hyacinthus Palau. eLife 10: e64790CrossrefCASPubMedGoogle Donovan MK, Burkepile Kratochwill Shlesinger T, Sully Oliver TA, Hodgson G, Freiwald van Woesik Local magnify heatwaves. 372: 977–980CrossrefCASPubMedWeb Eakin CM, Devotta Heron Connolly Liu Geiger Cour JDL, Gomez A, Skirving Baird 2014–17 event: destruction. https://doi.org/10.21203/rs.3.rs-1555992/v1CrossrefGoogle Hoegh-Guldberg O, Kennedy EV, Beyer H, McClennen Possingham HP Trends 33: 936–944CrossrefPubMedWeb Allemand Anthony Baker AC, Beck MW, Hale LZ, Hilmi Hughes Kaufman L Designing blueprint survival. Biol Conserv 257: 109107CrossrefWeb Corcoran Felis Goeij Grottoli Harding Mayfield Miller Obura D Rebuilding reefs: decadal grand challenge. Bremen: Future Earth Coasts, 56 ppCrossrefGoogle LaJeunesse TC, Parkinson Gabrielson PW, Jeong HJ, Reimer JD, CR, Santos Systematic revision highlights antiquity endosymbionts. Curr 28: 2570–2580.e6CrossrefCASPubMedWeb (2019) research increa

Language: Английский

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Invasive macroalgae shape chemical and microbial waterscapes on coral reefs

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: Jan. 6, 2025

Over the past decades, human impacts have changed structure of tropical benthic reef communities towards coral depletion and macroalgal proliferation. However, how these changes modified chemical microbial waterscapes is poorly known. Here, we assessed experimental removal assemblages influences composition two boundary layers, momentum. Chemical were spatially structured, both horizontally vertically, according to dominance layers. Microbes associated with degradation enriched in layers surrounding macroalgal-dominated substrata. Dominant macroalgae surrounded by a distinct pool diverse lipid classes (e.g., diterpenoids glycerolipids) labile organic matter organooxygen compounds), which diffused from algal tissues their polarity. Finally, our results highlighted strong co-variations between specific algal-derived metabolites planktonic microbes, giving insight into roles functioning resilience.

Language: Английский

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Microbiome Stability Is Linked to Acropora Coral Thermotolerance in Northwestern Philippines

Environmental Microbiology, Journal Year: 2025, Volume and Issue: 27(2)

Published: Jan. 31, 2025

Corals associate with a diverse community of prokaryotic symbionts that provide nutrition, antioxidants and other protective compounds to their host. However, the influence microbes on coral thermotolerance remains understudied. Here, we examined microbial communities associated colonies Acropora cf. tenuis exhibit high or low upon exposure 33°C (heated) relative 29°C (control). Using 16S rRNA sequencing, show structure all A. was similar each at control temperature. Thermotolerant colonies, however, had relatively greater abundance Endozoicomonas, Arcobacter, Bifidobacterium Lactobacillus. At elevated temperature, only thermosensitive showed distinct shift in microbiome, an increase Flavobacteriales, Rhodobacteraceae Vibrio, accompanying marked bleaching response. Functional prediction indicated thermotolerant corals were enriched for genes related metabolism, while microbiomes cell motility antibiotic compound synthesis. These differences may contribute variable performance under thermal stress. Identification taxa correlated provides insights into beneficial bacterial groups could be used microbiome engineering support reef health changing climate.

Language: Английский

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