Exploring the Potential Molecular Mechanisms of Interactions between a Probiotic Consortium and Its Coral Host

mSystems, Journal Year: 2023, Volume and Issue: 8(1)

Published: Jan. 23, 2023

Probiotics are currently the main hope as a potential medicine for corals, organisms that considered marine “canaries of coal mine” and threatened with extinction. Our experiments have proved concept probiotics mitigate coral bleaching can also prevent mortality.

Language: Английский

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Methods and Strategies to Uncover Coral-Associated Microbial Dark Matter

mSystems, Journal Year: 2022, Volume and Issue: 7(4)

Published: July 5, 2022

The vast majority of environmental microbes have not yet been cultured, and most the knowledge on coral-associated (CAMs) has generated from amplicon sequencing metagenomes. However, exploring cultured CAMs is key for a detailed comprehensive characterization roles these in shaping coral health and, ultimately, their biotechnological use as, example, probiotics other natural products. Here, strategies technologies that used to access are presented, while advantages disadvantages associated with each discussed.

Language: Английский

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Microbiome Engineering: A Promising Approach to Improve Coral Health

Engineering, Journal Year: 2022, Volume and Issue: 28, P. 105 - 116

Published: Aug. 20, 2022

The world's coral reefs are threatened by the cumulative impacts of global climate change and local stressors. Driven largely a desire to understand interactions between corals their symbiotic microorganisms, use this knowledge eventually improve health, interest in microbiology microbiome has increased recent years. In review, we summarize role maintaining healthy metaorganism providing nutrients, support for growth development, protection against pathogens, mitigation environmental We explore concept engineering, that is, precise controlled manipulation aid enhance resilience tolerance changing oceans. Although engineering is clearly its infancy, several breakthroughs indicate such an effective tool restoration preservation these valuable ecosystems. To assist with identifying future research targets, have reviewed common principles applications improving human health agricultural productivity, drawing parallels where can advance not-too-distant future. Finally, end discussing challenges faced researchers practitioners application provide recommendations work.

Language: Английский

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Antibiotics reduce Pocillopora coral‐associated bacteria diversity, decrease holobiont oxygen consumption and activate immune gene expression

Molecular Ecology, Journal Year: 2023, Volume and Issue: 32(16), P. 4677 - 4694

Published: June 15, 2023

Abstract Corals are important models for understanding invertebrate host–microbe interactions; however, to fully discern mechanisms involved in these relationships, experimental approaches manipulating coral–bacteria associations needed. Coral‐associated bacteria affect holobiont health via nutrient cycling, metabolic exchanges and pathogen exclusion, yet it is not understood how bacterial community shifts physiology. In this study, a combination of antibiotics (ampicillin, streptomycin ciprofloxacin) was used disrupt the communities 14 colonies reef framework‐building corals Pocillopora meandrina P . verrucosa, originally collected from Panama hosting diverse algal symbionts (family Symbiodiniaceae ). photochemical efficiencies oxygen consumption (as proxies coral health) were measured throughout 5‐day exposure. Antibiotics altered composition reduced alpha beta diversity, several persisted, leading hypothesis that either resistant or occupy internal niches shielded antibiotics. While did efficiency, antibiotics‐treated had lower rates. RNAseq revealed increased expression immunity stress response genes at expense cellular maintenance metabolism functions. Together, results reveal antibiotic disruption corals' native negatively impacts by decreasing activating host without directly impairing photosynthesis, underscoring critical role coral‐associated health. They also provide baseline future experiments manipulate symbioses first reducing diversity complexity bacteria.

Language: Английский

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Localization and symbiotic status of probiotics in the coral holobiont

mSystems, Journal Year: 2024, Volume and Issue: 9(5)

Published: April 12, 2024

ABSTRACT Corals establish symbiotic relationships with microorganisms, especially endosymbiotic photosynthetic algae. Although other microbes have been commonly detected in coral tissues, their identity and beneficial functions for host are unclear. Here, we confirm the outcomes of inoculation bacteria selected as probiotics use fluorescence situ hybridization (FISH) to define localization Pocillopora damicornis . Our results show first evidence inherent presence Halomonas sp. Cobetia native even before inoculation. Furthermore, relative enrichment these tissue-associated through corals correlates health improvements, such increases potential, productivity. study suggests status by indicating within gastrodermis epidermis correlating increased abundance active holobiont. This knowledge is crucial facilitate screening application that may not be transient members microbiome. IMPORTANCE Despite promising associated some scarce regarding bacterial cells found tissue, correlation between two aspects still missing. gap limits our understanding actual diversity coral-associated whether symbionts beneficial. Some researchers, example, suggesting probiotic should only focus on very few known bacteria, Endozoicomonas sp., assuming currently tested tissue-associated. provide specific FISH probes expand probiotics. The microorganisms (BMCs) inside tissues gastric cavities also supports notion direct interactions underpin role. a new breakthrough; argue against possibility positive effects BMCs due factors related interaction, simply feeds inoculated or change water quality.

Language: Английский

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Rapid Shifts in Bacterial Communities and Homogeneity of Symbiodiniaceae in Colonies of Pocillopora acuta Transplanted Between Reef and Mangrove Environments

Frontiers in Microbiology, Journal Year: 2021, Volume and Issue: 12

Published: Oct. 25, 2021

It has been proposed that an effective approach for predicting whether and how reef-forming corals persist under future climate change is to examine populations thriving in present day extreme environments, such as mangrove lagoons, where water temperatures can exceed those of reef environments by more than 3°C, pH levels are acidic (pH < 7.9, often below 7.6) O2 concentrations regularly considered hypoxic (<2 mg/L). Defining the physiological features these "extreme" corals, well their relationships with the, symbiotic, organisms within microbiome, could increase our understanding will into future. To better understand coral-microbe potentially underpin coral persistence we therefore conducted a 9-month reciprocal transplant experiment, whereby specimens Pocillopora acuta were transplanted between adjacent sites on northern Great Barrier Reef. Bacterial communities associated P. native environment dominated Endozoicomonas, while Symbiodiniaceae members Cladocopium genus. In contrast, colonies site exhibited highly diverse bacterial no dominating members, Durusdinium. All survived 9 months after being from reef-to-mangrove, mangrove-to-reef (as control transplants), during this time there significant changes communities, but not or photo-physiological functioning. reef-to-mangrove varied, sometimes rapid shifts including loss "core" began resemble corals. also changed original composition, remained different Our data demonstrates strongly influenced environmental conditions, whereas remain stable.

Language: Английский

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Probiotics for coral aquaculture: challenges and considerations

Current Opinion in Biotechnology, Journal Year: 2021, Volume and Issue: 73, P. 380 - 386

Published: Nov. 5, 2021

Language: Английский

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A roadmap to understanding diversity and function of coral reef-associated fungi

FEMS Microbiology Reviews, Journal Year: 2022, Volume and Issue: 46(6)

Published: June 23, 2022

Abstract Tropical coral reefs are hotspots of marine productivity, owing to the association reef-building corals with endosymbiotic algae and metabolically diverse bacterial communities. However, functional importance fungi, well-known for their contribution shaping terrestrial ecosystems global nutrient cycles, remains underexplored on reefs. We here conceptualize how fungal traits may have facilitated spread, diversification, ecological adaptation fungi propose that functions reef-associated be go beyond hitherto described roles pathogens bioeroders, including but not limited reef-scale biogeochemical cycles structuring coral-associated environmental microbiomes via chemical mediation. Recent technological conceptual advances will allow elucidation physiological, ecological, contributions understudied holobiont reef ecosystem functioning health help provide an outlook management actions.

Language: Английский

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Help Me, Symbionts, You're My Only Hope: Approaches to Accelerate our Understanding of Coral Holobiont Interactions

Integrative and Comparative Biology, Journal Year: 2022, Volume and Issue: 62(6), P. 1756 - 1769

Published: Sept. 13, 2022

Tropical corals construct the three-dimensional framework for one of most diverse ecosystems on planet, providing habitat to a plethora species across taxa. However, these ecosystem engineers are facing unprecedented challenges, such as increasing disease prevalence and marine heatwaves associated with anthropogenic global change. As result, major declines in coral cover health being observed world's oceans, often due breakdown coral-associated symbioses. Here, we review interactions between symbiotic partners holobiont-the cnidarian host, algae family Symbiodiniaceae, microbiome-that influence trait variation, including molecular mechanisms that underlie symbiosis resulting physiological benefits different microbial partnerships. In doing so, highlight current formation maintenance cnidarian-Symbiodiniaceae symbiosis, role immunity pathways play this relationship. We emphasize understanding complex is challenging when you consider vast genetic variation host algal symbiont, well their highly microbiome, which also an important player holobiont health. Given among partners, propose several research directions approaches focused model systems emerging technologies will broaden our how partner may facilitate prediction phenotype, especially under rapid environmental

Language: Английский

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Genome analysis of a coral-associated bacterial consortium highlights complementary hydrocarbon degradation ability and other beneficial mechanisms for the host

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: July 28, 2023

Abstract Here we report the oil degradation genetic potential of six oil-degrading bacteria (ODB), previously used as a bioremediation consortium, isolated from hydrocoral Millepora alcicornis and seawater. The strains were identified Halomonas sp. (LC_1), Cobetia (LC_6), Pseudoalteromonas shioyasakiensis (LC_2), Halopseudomonas aestusnigri (LC_3), Shewanella algae (LC_4), Brucella intermedia (LC_5). taxonomic identification differed that original paper when whole genome gene markers instead just 16S rRNA gene. Genes responsible for aromatic hydrocarbons n-alkanes found in all genomes, although different (and complementary) steps metabolic pathways unique to each strain. naphthalene toluene various strains. We annotated quinate genes LC_6, while LC_3 LC_5 presented biosurfactant rhamnolipid biosynthesis. also related beneficial mechanisms corals, such involved nitrogen DMSP metabolism, cobalamin biosynthesis antimicrobial compounds production. Our findings reinforce importance using bacterial consortia approaches single strains, due their complementary genomic arsenals. propose genome-based framework select ODB can provide additional benefits coral health.

Language: Английский

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