Endophytic communities in S. lycopersicum genotypes: Isolation of a novel fungal strain and its application to plant DOI Creative Commons

Luisa Liu Xu

Published: Sept. 25, 2023

Nowadays, due to the consequences of climate change and global overpopulation research in agricultural sector has become more crucial than ever.Although plants are sessile, they not unprotected.It is known that part a large ecological unit includes their microbiota -the microorganisms live contact with them.The presence these such as fungi bacteria varies depending on biological environmental factors.Different plant species interact different microbial communities, geological, climatic biotic conditions can influence interactions.The affect growth, well its interaction environment resistance abiotic stresses.Within this context, endophytes, inside tissues, gaining prominence alternatives combat various stresses may face.The use endophytic organisms agronomic improvement broad field study which made significant advances recent decades.However, most literature reviews tend focus specific aspects research, particular species.For reason, we have conducted comprehensive analysis general found scientific up 2021.The shown vast majority studies date focused ascomycetes.More half analyzed related herbaceous stress.Meanwhile, despite several articles proving dicotyledonous crops potential effect inducers against pests, remains relatively unexplored.These results allowed us direct our towards less frequently studied areas promising for exploitation.On other hand, previous demonstrated genotypes same exhibit distinct behaviors development response adverse conditions.This information particularly relevant, since varieties greater tolerance could be highly beneficial contemporary agriculture.In light this, characterized six 低番茄中 Pst 感染的水平。此外，我们还对植物样本进行了转录组学和激素分析，

Language: Английский

High-throughput DNA extraction and cost-effective miniaturized metagenome and amplicon library preparation of soil samples for DNA sequencing DOI Creative Commons
Thomas Bygh Nymann Jensen, Sebastian Mølvang Dall,

Simon Knutsson

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(4), P. e0301446 - e0301446

Published: April 4, 2024

Reductions in sequencing costs have enabled widespread use of shotgun metagenomics and amplicon sequencing, which drastically improved our understanding the microbial world. However, large projects are now hampered by cost library preparation low sample throughput, comparatively to actual costs. Here, we benchmarked three high-throughput DNA extraction methods: ZymoBIOMICS™ 96 MagBead Kit, MP Biomedicals TM FastDNA -96 Soil Microbe DNeasy® PowerSoil® Pro QIAcube® HT Kit. The extractions were evaluated based on length, quality, quantity, observed community across five diverse soil types. all types was successful for kits, however Kit excelled performance parameters. We further used nanoliter dispensing system I.DOT One miniaturize Illumina metagenomic volumes a factor 5 10, respectively, with no significant impact communities. With these protocols, extraction, metagenomic, or one 96-well plate approx. 3, 5, 6 hours, respectively. Furthermore, miniaturization metagenome reduces chemical plastic from 5.0 3.6 59 7.3 USD pr. sample. This enhanced efficiency cost-effectiveness will enable researchers undertake studies greater sizes diversity, thereby providing richer, more detailed view communities their dynamics.

Language: Английский

Citations

7
Effects of the Continuous Cropping of Amomum villosum on Rhizosphere Soil Physicochemical Properties, Enzyme Activities, and Microbial Communities DOI Creative Commons

Butian Wang,

Yunfeng Lu,

Weifeng Li

et al.

Agronomy, Journal Year: 2022, Volume and Issue: 12(10), P. 2548 - 2548

Published: Oct. 18, 2022

Amomum villosum, which is an important perennial medicinal plant, easily suffers from continuous cropping obstacles in the plantation. The aim of this study to find effective method solve problem A. villosum cropping. In study, we analyzed four fields was continuously cropped and a fallow field reveal effects on rhizosphere soil physicochemical properties, enzyme activities, bacterial fungal communities. Most nutrient contents gradually increased as number years increased, whereas pH decreased slightly. urease acid phosphatase activities tended increase length period may have accelerated conversion substances. Furthermore, alpha diversity communities duration increased. Additionally, redundancy analysis revealed that community structures at phylum level were most correlated with value catalase activity, respectively. This be useful for promoting sustainable development villosum.

Language: Английский

Citations

25
Plant Growth-Promoting Microorganisms as Biocontrol Agents: Mechanisms, Challenges, and Future Prospects DOI Creative Commons
Seham M. Al Raish,

Osama M. Sourani,

Abdelghafar M. Abu-Elsaoud

et al.

Applied Microbiology, Journal Year: 2025, Volume and Issue: 5(2), P. 44 - 44

Published: May 5, 2025

The escalating threats posed by plant pathogens and the environmental repercussions of conventional agrochemicals necessitate sustainable agricultural solutions. This review focuses on growth-promoting microorganisms (PGPMs) such as bacteria, filamentous fungi, yeasts, which play a pivotal role biocontrol agents. These organisms enhance growth resilience through nutrient solubilization, phytohormone production, antagonistic activities against pathogens, offering dual benefit disease suppression enhancement. However, effective application PGPMs faces challenges, including variability in field performance, survival colonization under conditions, regulatory hurdles. paper discusses these challenges explores recent advances utilizing bioagents agriculture, underscoring importance integrated pest management systems that reduce chemical inputs, thus promoting ecological balance farming practices.

Language: Английский

Citations

0
Effect of Different Cultivation Patterns on Amomum villosum Yield and Quality Parameters, Rhizosphere Soil Properties, and Rhizosphere Soil Microbes DOI Creative Commons

Butian Wang,

Hongmei Chen,

Peng Qu

et al.

Horticulturae, Journal Year: 2023, Volume and Issue: 9(3), P. 306 - 306

Published: Feb. 23, 2023

The forest–medicinal plant management system has benefited the commercial production of Amomum villosum. However, little is known about influence different forestlands on cultivation A. present study investigated potential differences in villosum yield and quality parameters, rhizosphere soil properties, microbiota between a rubber plantation (RP) natural secondary forest (NSF). No significant or properties were observed RP NSF, although most physicochemical enzyme activities higher NSF than RP. Furthermore, 38 volatile components had significantly relative abundances alpha diversity indices for communities indicated that richness bacterial fungal was These findings suggest conditions may be more appropriate growing data generated this useful increasing high-quality via exploitation environments.

Language: Английский

Citations

5
Analysis of the endophytic microbiota of roots and culms of two commercial sugarcane cultivars inoculated with a synthetic microbial community DOI
Stefan Schwab,

Araceli de Souza Pires,

Giovanna Zuzarte Candido

et al.

Applied Soil Ecology, Journal Year: 2023, Volume and Issue: 195, P. 105235 - 105235

Published: Dec. 6, 2023

Language: Английский

Citations

4
Sugarcane rhizosphere microbiota: exploring diversity across varied topographies and growth environments DOI
Bin Wang, Xihui Liu, Yanhua Qi

et al.

Plant and Soil, Journal Year: 2024, Volume and Issue: unknown

Published: May 6, 2024

Language: Английский

Citations

1
Exploring the impact of plant genotype and fungicide treatment on endophytic communities in tomato stems DOI Creative Commons
Luisa Liu-Xu, Liang Ma, Atefeh Farvardin

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 25, 2024

This study examines how plant genotype can influence the microbiome by comparing six tomato genotypes ( Solanum lycopersicum ) based on their traditional vs. commercial backgrounds. Using Illumina-based sequencing of V6-V8 regions 16S and ITS2 rRNA genes, we analyzed compared endophytic bacterial fungal communities in stems to understand microbiota differ be altered relation human manipulation. Our results reflect that harbor significantly more exclusive microbial taxa a broader phylogenetic background than ones. Traditional were richer Eurotiomycetes Sordariomycetes fungi, while Lasiosphaeriaceae was prevalent genotypes. TH-30 exhibited highest abundance, genotypes, particularly Actinomycetia, Bacteroidia , Gammaproteobacteria . Additionally, had higher diversity, notably orders like Cytophagales, Xanthomonadales Burkholderiales Moreover, performed an evaluation impact systemic fungicide (tebuconazole-dichlofluanide) simulate common agronomic practice determined single treatment stem microbiota. Control plants prevalence Pleosporales, Helotiales Glomerellales treated dominated Laboulbeniomycetes Fungal community diversity decreased, but no significant observed diversity. provides evidence variety impacts endophytes. Furthermore, these findings suggest potential benefits using as source novel beneficial may prove highly valuable unpredicted challenges advancement sustainable agriculture.

Language: Английский

Citations

1
High-throughput DNA extraction and cost-effective miniaturized metagenome and amplicon library preparation of soil samples for DNA sequencing DOI Creative Commons
Thomas Bygh Nymann Jensen, Sebastian Mølvang Dall,

Simon Knutsson

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 4, 2023

Abstract Reductions in sequencing costs have enabled widespread use of shotgun metagenomics and amplicon sequencing, which drastically improved our understanding the microbial world. However, large projects are now hampered by cost library preparation low sample throughput. Here, we benchmarked three high-throughput DNA extraction methods: ZymoBIOMICS™ 96 MagBead Kit, MP Biomedicals TM FastDNA -96 Soil Microbe DNeasy® PowerSoil® Pro QIAcube® HT Kit. The extractions were evaluated based on length, quality, quantity, observed community across five diverse soil types. all types was successful for kits, however Kit excelled performance parameters. We further used nanoliter dispensing robot I.DOT One to miniaturize Illumina metagenomic volumes a factor 5 10, respectively, with no significant impact communities. With these protocols, extraction, preparation, or one 96-well plate approx. 3, 5, 6 hours, respectively. Furthermore, miniaturization metagenome reduces chemical plastic from 5.0 3.6 59 7.3 USD pr. sample.

Language: Английский

Citations

1
Eficiência do uso de fungicidas químicos e biológicos na cana-de-açúcar DOI Creative Commons

Mayra Alves do Nascimento,

Eugénia Cardoso, Lísia Daltro Borges Alves

et al.

Revista de Gestão e Secretariado (Management and Administrative Professional Review), Journal Year: 2024, Volume and Issue: 15(10), P. e4258 - e4258

Published: Oct. 25, 2024

Esse estudo teve como objetivo avaliar a resposta da aplicação do fungicida químico e biológico suas ações no controle de doenças, sob o desenvolvimento viabilidade econômica na cana-de-açúcar. O delineamento experimental adotado foi blocos casualizados com seis tratamentos, quatro repetições, totalizando 24 parcelas. Os dados foram submetidos análise variância pelo teste F as médias comparadas Scott-Knott (p≤0,05). A composição produto é (Bacillus pumilus CNPSo 3203; Bacillus velezensis 3602 subtilis 2720) B (AZOXITROBINA 120 g/L TEBUCONAZOLE 200 g L-1). tratamentos foram: T1- Controle; T2- PRODUTO dose 0,3 L ha-1; T3- 0,75 T4- ha-1 + em segunda T5- primeira T6- ha-1. variedade RB041443, avaliações severidade doença, altura planta (AP), diâmetro caule (DC), produtividade realizadas Teor Sólidos Solúveis (°Brix) Tonelada cana por hectare (TCH). resultados ambos fungicidas são eficientes patógenos cana-de-açúcar, os T2 T4 mais expressivos para variável teor sólidos solúveis, maiores valores TCH obtidos T5 T6 nestes relação testemunha.Planta, largura planta, número folhas caulinar. Não observadas diferenças nas trocas gasosas das doenças entre cultivares algodoeiro.

Citations

0
Rhizosphere functioning and rhizo-microbiome of two contrasting genotypes of rice under drought-induced conditions DOI Creative Commons
Sai Aparna Devi Nunna, Dananjeyan Balachandar

Research Square (Research Square), Journal Year: 2022, Volume and Issue: unknown

Published: Nov. 14, 2022

Abstract Background and aim Rhizosphere function is the key determinant of crop growth fitness under moisture-stress conditions. Human-centered breeding adversely affects microbiome recruitment crops. Hence, during drought, wild types landraces adapt to stress, while modern cultivars hybrids fail do so are susceptible drought. Understanding rhizosphere difference between will help improve microbiome-mediated drought mitigation. Methods We compared rhizospheres rice landrace, Norungan, high-yielding cultivar, Co51, grown normal moisture drought-induced conditions using soil biochemical 16S rRNA gene sequencing approach. Results Soil carbon pools, enzymes, respiration were affected due in both genotypes' rhizospheres. However, Norungan accounted for less harm attributes than Co51. Reduction organic (3.94%), microbial biomass (14.26%), labile (1.94%), dehydrogenase (10.1%), urease (21.27%), phosphatase (9.61%), rate (15.02%) was Co51 Alpha diversity bacterial communities rice's significantly lower bulk soil, further reduced genotypes. Moisture-stress abundance Firmicutes (180%) Bacteroidetes (57%) Norungan's rhizosphere, while, Acidobacteria (51%), Actinobacteria (54%), Chlolorflexi (41%), Proteobacteria (34%) got increased. On other hand, acquired an enhanced (79%) Bacteriodetes (170%) lessened (15%) (31%) Conclusion These results suggest that landrace recruits less-diversified, specific groups microorganisms augment functioning.

Language: Английский

Citations

2