Molecular Engineering of Alginate Lyases and the Potential Agricultural Applications of Their Enzymatic Products

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 26, 2025

Alginate lyases, enzymes that degrade alginate into unsaturated oligosaccharides, have attracted significant attention for their potential applications across various fields, particularly in agriculture. This review focuses on the molecular engineering of lyases to enhance activity, stability, and specificity as well agricultural resulting enzymatic products, known oligosaccharides (AOS). We start by summarizing sources classification followed recent advances through directed evolution, rational design, truncation noncatalytic domains, conserved domain reconstruction. then explore diverse AOS, including ability promote plant growth, increase content active components, extend fruit shelf life, resistance abiotic stresses. Furthermore, value AOS feed additives preservatives shrimp-based products is also assessed. will not only lay a solid theoretical foundation but serve catalyst innovative development practical application high-value preparations utilization AOS-related providing new solutions sustainable agriculture food industry.

Language: Английский

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Physiological and Molecular Mechanisms of Rice Tolerance to Salt and Drought Stress: Advances and Future Directions

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9404 - 9404

Published: Aug. 29, 2024

Rice, a globally important food crop, faces significant challenges due to salt and drought stress. These abiotic stresses severely impact rice growth yield, manifesting as reduced plant height, decreased tillering, biomass, poor leaf development. Recent advances in molecular biology genomics have uncovered key physiological mechanisms that employs cope with these stresses, including osmotic regulation, ion balance, antioxidant responses, signal transduction, gene expression regulation. Transcription factors such DREB, NAC, bZIP, well hormones like ABA GA, been identified crucial regulators. Utilizing CRISPR/Cas9 technology for editing holds promise significantly enhancing stress tolerance. Future research should integrate multi-omics approaches smart agriculture technologies develop varieties enhanced resistance, ensuring security sustainable the face of global environmental changes.

Language: Английский

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Effects of Exogenous Isosteviol on the Physiological Characteristics of Brassica napus Seedlings under Salt Stress

Plants, Journal Year: 2024, Volume and Issue: 13(2), P. 217 - 217

Published: Jan. 12, 2024

In this paper, the effect of isosteviol on physiological metabolism

Language: Английский

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Alginate Oligosaccharides Alleviate Salt Stress in Rice Seedlings by Regulating Cell Wall Metabolism to Maintain Cell Wall Structure and Improve Lodging Resistance

Plants, Journal Year: 2024, Volume and Issue: 13(9), P. 1215 - 1215

Published: April 28, 2024

Salt stress is one of the major abiotic stresses that damage structure and composition cell walls. Alginate oligosaccharides (AOS) have been advocated to significantly improve plant tolerance. The metabolic mechanism by which AOS induces salt tolerance in rice walls remains unclear. Here, we report impact foliar application on wall seedlings using salt-tolerant variety FL478 salt-sensitive IR29. Data revealed decreased biomass, stem basal width, breaking strength, lodging resistance; however, it increased thickness. In leaves, exogenous up-regulated expression level OSCESA8, abscisic acid (ABA) brassinosteroids (BR) content, β-galacturonic activity, polygalacturonase xylanase laccase cellulose content. Moreover, down-regulated levels OSMYB46 OSIRX10 hemicellulose, pectin, lignin content maintain stability under stress. stems, phenylalamine ammonia-lyase tyrosine activities, while decreasing cellulase, laccase, β-glucanase activities. Furthermore, improved biomass width also enhanced cellulose, stem, As a result, resistance breakage strength alleviated stress-induced damage, thus enhancing resistance. Under stress, regulates phytohormones modifies lignin, pectin metabolism lodging. This study aims alleviate walls, enhance lodging, AOS.

Language: Английский

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Integrated transcriptome and metabolome analysis of salinity tolerance in response to foliar application of choline chloride in rice (Oryza sativa L.)

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 1, 2024

Introduction Salt stress is a major abiotic that affects crop growth and productivity. Choline Chloride (CC) has been shown to enhance salt tolerance in various crops, but the underlying molecular mechanisms rice remain unclear. Methods To investigate regulatory mechanism of CC-mediated rice, we conducted morpho-physiological, metabolomic, transcriptomic analyses on two varieties (WSY, salt-tolerant, HHZ, salt-sensitive) treated with 500 mg·L -1 CC under 0.3% NaCl stress. Results Our results showed foliar application improved morpho-physiological parameters such as root traits, seedling height, strength index, fullness, leaf area, photosynthetic parameters, pigments, starch, fructose content stress, while decreasing soluble sugar, sucrose, sucrose phosphate synthase levels. Transcriptomic analysis revealed regulation combined treatment induced changes expression genes related starch metabolism, citric acid cycle, carbon sequestration organs, antenna proteins both varieties. Metabolomic further supported these findings, indicating photosynthesis, fixation pathways were crucial tolerance. Discussion The metabolomic data suggest enhances by activating distinct transcriptional cascades phytohormone signaling, along multiple antioxidants unique metabolic pathways. These findings provide basis for understanding metabolite synthesis gene response may inform strategies improving resilience

Language: Английский

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Comparative transcriptomic analysis of the regulatory mechanisms underlying the resistance to Fusarium foetens mediated by konjac glucomannan oligosaccharides in tobacco

Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102585 - 102585

Published: Jan. 1, 2025

Language: Английский

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Growth and physiological characteristics of forage bermudagrass in response to salt stress

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 1, 2025

Bermudagrass (Cynodon dactylon) has a long history as an excellent forage grass, and salt stress will inhibit its growth development. In order to minimize the damage, it is necessary continuously develop innovative technologies management strategies. This study evaluated tolerance of new strains 'FB2019R101' 'FB2019R105' compared commercial varieties 'Wrangler' 'A12359' under simulated soil salinity conditions through seawater irrigation. Through correlation analysis growth, physiological, nutritional indicators, principal component analysis, core indicators weights for evaluation were identified. The salt-tolerant 'FB2019R105'. Under stress, plants with suffered less damage whole, which could better regulate osmotic balance inside outside cells, accumulate more nutrients have stronger ability resist damage. expression level variety CdCINV1, CdSPS1, CdSUS5, CdSWEET6 was up-regulated stress. CdSUS5 can promote transformation sucrose into glucose fructose in accumulation fructose. exhibited higher tolerance, minimal impact on their biomass, comprehensive revealed ranking > 'A12359'. provides significant reference bioremediation coastal saline soils promotes research application conditions. improve by regulating changes carbohydrates.

Language: Английский

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Unveiling Salt Tolerance Mechanisms in Plants: Integrating the KANMB Machine Learning Model With Metabolomic and Transcriptomic Analysis

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

Abstract Salt stress presents a substantial threat to cereal crop productivity, especially in coastal agricultural regions where salinity levels are high. Addressing this challenge requires innovative approaches uncover genetic resources that support molecular breeding of salt‐tolerant crops. In study, novel machine learning model, KANMB is introduced, designed analyze integrated multi‐omics data from the natural halophyte Spartina alterniflora under various NaCl concentrations. Using KANMB, 226 metabolic biomarkers significantly linked salt responses, grounded metabolomic and transcriptomic profiles identified. These correlate with pathways associated tolerance, providing insight into underlying biochemical mechanisms. A co‐expression analysis further highlights MYB gene SaMYB35 as pivotal regulator flavonoid biosynthesis pathway stress. When overexpressed rice (ZH11) grown high salinity, it triggers upregulation key biosynthetic genes, elevates content, enhances tolerance compared wild‐type plants. The findings study offer valuable toolkit for varieties demonstrate power accelerating biomarker discovery resilience non‐model plant species.

Language: Английский

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Recent Advances in Alginate Lyase Engineering for Efficient Conversion of Alginate to Value‐Added Products

Microbial Biotechnology, Journal Year: 2025, Volume and Issue: 18(5)

Published: April 28, 2025

ABSTRACT Alginate lyases depolymerize alginate and generate oligosaccharides (AOS) eventually 4‐deoxy‐L‐erythro‐5‐hexoseulose uronate (DEH), a monosaccharide. Recently, have garnered significant attention due to the increasing demand for AOS, which exhibit bioactivities beneficial human health, livestock productivity, agricultural efficiency. Additionally, these enzymes play crucial role in producing DEH, essential catabolism bacteria. This review explains industrial value of AOS contribute broadly industries ranging from food industry biorefinery processes. also highlights recent advances lyase applications engineering, including domain truncation, chimeric enzyme design, rational mutagenesis, directed evolution. These approaches enhanced performance efficient DEH production. We discuss current challenges future directions toward industrial‐scale bioconversion alginate‐rich biomass.

Language: Английский

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Comparative transcriptomic and metabolomic analyses provide insights into the responses to high temperature stress in Alfalfa (Medicago sativa L.)

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Aug. 15, 2024

High temperature stress is one of the most severe forms abiotic in alfalfa. With intensification climate change, frequency high will further increase future, which bring challenges to growth and development Therefore, untargeted metabolomic RNA-Seq profiling were implemented unravel possible alteration alfalfa seedlings subjected different (25 ℃, 30 35 40 ℃) this study. Results revealed that significantly altered some pivotal transcripts metabolites. The number differentially expressed genes (DEGs) markedly up down-regulated was 1876 1524 T30_vs_CK, 2, 815 2667 T35_vs_CK, 2115 226 T40_vs_CK, respectively. for up-regulated differential metabolites 173 73 188 57 220 66 It worth noting metabolomics transcriptomics co-analysis characterized enriched plant hormone signal transduction (ko04705), glyoxylate dicarboxylate metabolism (ko00630), from participated. In particular, content changed under T40 stress, suggesting maintaining normal synthesis may be an important way improve HTS tolerance qRT-PCR showed expression pattern similar abundance transcriptome. This study provides a practical in-depth perspective investigating effects conferred by on development, provided theoretical basis breeding heat-resistant

Language: Английский

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