Photosynthesis drives retention of bacterial-like tRNA metabolism in plant organelles DOI Creative Commons
Rachael Ann DeTar,

Joanna Chustecki,

Anna Martinez-Hottovy

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 2, 2023

Abstract Eukaryotic nuclear genomes often encode distinct sets of translation machinery for function in the cytosol vs. organelles (mitochondria and plastids). This raises questions about why multiple systems are maintained even though they capable comparable functions whether evolve differently depending on compartment where operate. These particularly interesting plants because machinery, including aminoacyl-tRNA synthetases (aaRS), is dual-targeted to plastids mitochondria. have different functions, with much higher rates supply abundant, rapid-turnover proteins required photosynthesis. Previous studies indicated that plant organellar aaRS more slowly compared mitochondrial eukaryotes lack plastids. Thus, we investigated evolution nuclear-encoded cytosolic tRNA maturation enzymes across a broad sampling angiosperms, non-photosynthetic (heterotrophic) species reduced plastid gene expression, test hypothesis translational demands associated photosynthesis constrain involved metabolism. Remarkably, heterotrophic exhibited wholesale loss many organelle-targeted other enzymes, still occurs their mitochondria losses were accompanied by apparent retargeting tRNAs organelles, sometimes preserving aaRS-tRNA charging relationships but times creating surprising mismatches between substrates. Our findings indicate presence photosynthetic drives retention specialized Significance The process which endosymbionts integrated into host become results combination loss, transfer nucleus, genome. It not clear some endosymbiont-derived genes may be retained when functional counterpart exists whose product could simply retargeted organelles. study revealed activity responsible functionally redundant processing while flexible regarding substitution cytosolic-type enzymes. Therefore, constraint likely important than shaping dual targeted both

Language: Английский

Photosynthetic demands on translational machinery drive retention of redundant tRNA metabolism in plant organelles DOI Creative Commons
Rachael Ann DeTar, Joanna M. Chustecki,

Ana Martinez-Hottovy

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(52)

Published: Dec. 18, 2024

Eukaryotic nuclear genomes often encode distinct sets of translation machinery for function in the cytosol vs. organelles (mitochondria and plastids). This raises questions about why multiple systems are maintained even though they capable comparable functions whether evolve differently depending on compartment where operate. These particularly interesting plants because machinery, including aminoacyl-transfer RNA (tRNA) synthetases (aaRS), is dual-targeted to plastids mitochondria. have different functions, with much higher rates supply abundant, rapid-turnover proteins required photosynthesis. Previous studies indicated that plant organellar aaRS more slowly compared mitochondrial eukaryotes lack plastids. Thus, we investigated evolution nuclear-encoded cytosolic tRNA maturation enzymes across a broad sampling angiosperms, nonphotosynthetic (heterotrophic) species reduced plastid gene expression, test hypothesis translational demands associated photosynthesis constrain involved metabolism. Remarkably, heterotrophic exhibited wholesale loss many organelle-targeted other enzymes, still occurs their mitochondria losses were accompanied by apparent retargeting tRNAs organelles, sometimes preserving aaRS–tRNA charging relationships but times creating surprising mismatches between substrates. Our findings indicate presence photosynthetic drives retention specialized

Language: Английский

Citations

2
Photosynthesis drives retention of bacterial-like tRNA metabolism in plant organelles DOI Creative Commons
Rachael Ann DeTar,

Joanna Chustecki,

Anna Martinez-Hottovy

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 2, 2023

Abstract Eukaryotic nuclear genomes often encode distinct sets of translation machinery for function in the cytosol vs. organelles (mitochondria and plastids). This raises questions about why multiple systems are maintained even though they capable comparable functions whether evolve differently depending on compartment where operate. These particularly interesting plants because machinery, including aminoacyl-tRNA synthetases (aaRS), is dual-targeted to plastids mitochondria. have different functions, with much higher rates supply abundant, rapid-turnover proteins required photosynthesis. Previous studies indicated that plant organellar aaRS more slowly compared mitochondrial eukaryotes lack plastids. Thus, we investigated evolution nuclear-encoded cytosolic tRNA maturation enzymes across a broad sampling angiosperms, non-photosynthetic (heterotrophic) species reduced plastid gene expression, test hypothesis translational demands associated photosynthesis constrain involved metabolism. Remarkably, heterotrophic exhibited wholesale loss many organelle-targeted other enzymes, still occurs their mitochondria losses were accompanied by apparent retargeting tRNAs organelles, sometimes preserving aaRS-tRNA charging relationships but times creating surprising mismatches between substrates. Our findings indicate presence photosynthetic drives retention specialized Significance The process which endosymbionts integrated into host become results combination loss, transfer nucleus, genome. It not clear some endosymbiont-derived genes may be retained when functional counterpart exists whose product could simply retargeted organelles. study revealed activity responsible functionally redundant processing while flexible regarding substitution cytosolic-type enzymes. Therefore, constraint likely important than shaping dual targeted both

Language: Английский

Citations

1