Enhancing the efficacy of Zinc Oxide Nanoparticles by Beta-carotene conjugation for improved anti-microbial and anti-tumor therapy for Dental Application DOI
Mohammed Rafi Shaik, Siva Prasad Panda, Shaik Althaf Hussain

et al.

Pharmaceutical Development and Technology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 34

Published: Dec. 31, 2024

The increasing prevalence of dental pathogens and oral cancer calls for new therapeutic agents. Nanoparticle (NPs) based tumor therapy enables precise targeting controlled drug release, improving anti-cancer treatment efficacy while reducing systemic toxicity. Zinc oxide NPs (ZnO NPs) are notable in nanomedicine their exceptional physicochemical biological properties. This study synthesizes characterizes beta-carotene-coated ZnO (BT-ZnO potential antimicrobial applications, demonstrating significant against cells. Scanning Electron Microscopy, EDAX, UV, FTIR, XRD, Zeta analysis prepared BT-ZnO revealed uniform flower-like crystalline structures with intricate morphology an average particle size 38.06 nm. FTIR spectra identified various functional groups, suggesting a complex organic compound coated NPs. measurements showed pH-dependent surface charge variations, which crucial understanding colloidal stability. activity was potent pathogens, minimum inhibitory concentration (MIC) values 50 µg/mL highlighting inhibition. Molecular docking studies demonstrated strong binding affinities BT to key receptor proteins pathogens. exhibited antioxidant 68%, comparable ascorbic acid, anti-inflammatory effects 75.1% at 100 µg/mL. Cytotoxicity assays indicated concentration-dependent suppression KB cell proliferation, decreasing viability 37.19%, gene expression elevated P53 expression, apoptotic response. These multifaceted properties underscore the as integrated approach healthcare oncology.

Language: Английский

Eco-friendly synthesis of betanin-conjugated zinc oxide nanoparticles: antimicrobial efficacy and apoptotic pathway activation in oral cancer cells DOI
Chinnasamy Ragavendran,

Mohamed Imath,

Chinnaperumal Kamaraj

et al.

Molecular Biology Reports, Journal Year: 2024, Volume and Issue: 51(1)

Published: Nov. 7, 2024

Language: Английский

Citations

2
A Dual Approach by Nanostructured α‐Mangostin‐Copper Oxide Complexes Against Dental Pathogen Biofilms and Oral Cancer via Apoptosis Gene Modulation DOI
Mohammed Rafi Shaik, Mohankumar Ramasamy, Divya Jain

et al.

Chemistry & Biodiversity, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 22, 2024

Abstract The development of effective treatments for dental pathogens and oral cancer remains a significant challenge. Copper oxide nanoparticles (CuO NPs) are recognized their strong antimicrobial properties, attributed to the synthesis oxygen‐dependent radicals. α‐Mangostin (MG), natural xanthone from mangosteen fruit, is well‐known its antioxidant, antimicrobial, anticancer potential. combination CuO NPs with MG would offer synergistic approach enhance therapeutic efficacy. CuO‐MG were synthesized characterized size, morphology, surface properties. efficacy these was tested against pathogens, including Staphylococcus aureus , Enterococcus faecalis Streptococcus mutans Candida albicans . Antioxidant activity assessed using superoxide anion hydroxyl radical anion. potential evaluated by examining apoptosis induction in cell lines, focusing on expression key apoptotic markers such as Caspase‐3, Caspase‐8, FasL. Molecular docking simulations performed understand interaction between biofilm receptors. evidenced all enhanced combined effects CuO‐induced oxidative stress properties MG. assays demonstrated dose‐dependent increase scavenging activity. In cells, significantly reduced viability induced apoptosis, up‐regulation studies revealed binding affinities receptors, disrupting pathogen adhesion formation. offers powerful multifaceted healthcare. demonstrate offering management infections cancer. Further preclinical clinical recommended ensure safety stability medical applications.

Language: Английский

Citations

0
Synergistic Action of Rutin‐Coated Zinc Oxide Nanoparticles: Targeting Biofilm Formation Receptors of Dental Pathogens and Modulating Apoptosis Genes for Enhanced Oral Anticancer Activity DOI
Mohammed Rafi Shaik, Mohankumar Ramasamy, Divya Jain

et al.

Journal of Biochemical and Molecular Toxicology, Journal Year: 2024, Volume and Issue: 38(11)

Published: Oct. 29, 2024

ABSTRACT Oral diseases are often associated with bacterial and fungal pathogens such as Staphylococcus aureus , Streptococcus mutans Enterococcus faecalis Candida albicans . This research explored a novel approach to addressing these by synthesizing zinc oxide nanoparticles (ZnO NPs) coated rutin (RT), plant‐derived compound. The synthesized ZnO‐RT NPs were comprehensively characterized using UV‐Vis spectrophotometer, SEM, EDAX techniques confirm their structural composition. antioxidant potential was assessed through free radical scavenging assays. Additionally, the antimicrobial activity of evaluated zone inhibition assay against oral pathogens. Molecular docking studies Autodock tool performed elucidate interactions between RT receptors findings demonstrated that exhibited robust activity. Furthermore, they showed significant minimal inhibitory concentration 40 μg/mL also displayed dose‐dependent anticancer effects on human cancer cells at concentrations 10, 20, 40, 80 μg/mL. Mechanistic insights into KB revealed upregulation apoptotic genes. study underscores promising for dental applications due strong antioxidant, anticancer, properties. These offer hopeful prospect pathogen challenges enhancing overall health.

Language: Английский

Citations

0
Metagenomics approaches in the discovery and development of new bioactive compound of 8-demethoxy-10-deoxysteffimycin from mangrove sediments DOI

P. P. Sujith,

Jyoti Singh,

S. Jayalakshmi

et al.

3 Biotech, Journal Year: 2024, Volume and Issue: 14(12)

Published: Nov. 17, 2024

Language: Английский

Citations

0
Enhancing the efficacy of Zinc Oxide Nanoparticles by Beta-carotene conjugation for improved anti-microbial and anti-tumor therapy for Dental Application DOI
Mohammed Rafi Shaik, Siva Prasad Panda, Shaik Althaf Hussain

et al.

Pharmaceutical Development and Technology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 34

Published: Dec. 31, 2024

The increasing prevalence of dental pathogens and oral cancer calls for new therapeutic agents. Nanoparticle (NPs) based tumor therapy enables precise targeting controlled drug release, improving anti-cancer treatment efficacy while reducing systemic toxicity. Zinc oxide NPs (ZnO NPs) are notable in nanomedicine their exceptional physicochemical biological properties. This study synthesizes characterizes beta-carotene-coated ZnO (BT-ZnO potential antimicrobial applications, demonstrating significant against cells. Scanning Electron Microscopy, EDAX, UV, FTIR, XRD, Zeta analysis prepared BT-ZnO revealed uniform flower-like crystalline structures with intricate morphology an average particle size 38.06 nm. FTIR spectra identified various functional groups, suggesting a complex organic compound coated NPs. measurements showed pH-dependent surface charge variations, which crucial understanding colloidal stability. activity was potent pathogens, minimum inhibitory concentration (MIC) values 50 µg/mL highlighting inhibition. Molecular docking studies demonstrated strong binding affinities BT to key receptor proteins pathogens. exhibited antioxidant 68%, comparable ascorbic acid, anti-inflammatory effects 75.1% at 100 µg/mL. Cytotoxicity assays indicated concentration-dependent suppression KB cell proliferation, decreasing viability 37.19%, gene expression elevated P53 expression, apoptotic response. These multifaceted properties underscore the as integrated approach healthcare oncology.

Language: Английский

Citations

0