Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

European Journal of Immunology, Journal Year: 2019, Volume and Issue: 49(10), P. 1457 - 1973

Published: Oct. 1, 2019

These guidelines are a consensus work of considerable number members the immunology and flow cytometry community. They provide theory key practical aspects enabling immunologists to avoid common errors that often undermine immunological data. Notably, there comprehensive sections all major immune cell types with helpful Tables detailing phenotypes in murine human cells. The latest techniques applications also described, featuring examples data can be generated and, importantly, how analysed. Furthermore, tips, tricks pitfalls avoid, written peer-reviewed by leading experts field, making this an essential research companion.

Language: Английский

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Macrophages: versatile players in renal inflammation and fibrosis

Nature Reviews Nephrology, Journal Year: 2019, Volume and Issue: 15(3), P. 144 - 158

Published: Jan. 28, 2019

Language: Английский

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Macrophage Polarization in Physiological and Pathological Pregnancy

Frontiers in Immunology, Journal Year: 2019, Volume and Issue: 10

Published: April 15, 2019

The immunology of pregnancy is complex and poorly defined. During the process pregnancy, macrophages secrete many cytokines/chemokines play pivotal roles in maintenance maternal-fetal tolerance. Here, we summarized current knowledge macrophage polarization mechanisms involved physiological or pathological processes, including miscarriage, preeclampsia, preterm birth. Although evidence provides a compelling argument that are important our understanding still rudimentary. Since exhibit functional plasticity, they may be ideal targets for therapeutic manipulation during pregnancy. Additional studies needed to better define functions various subsets both normal

Language: Английский

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The human heart contains distinct macrophage subsets with divergent origins and functions

Nature Medicine, Journal Year: 2018, Volume and Issue: 24(8), P. 1234 - 1245

Published: June 8, 2018

Language: Английский

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Skull and vertebral bone marrow are myeloid cell reservoirs for the meninges and CNS parenchyma

Science, Journal Year: 2021, Volume and Issue: 373(6553)

Published: June 3, 2021

The meninges are a membranous structure enveloping the central nervous system (CNS) that host rich repertoire of immune cells mediating CNS surveillance. Here, we report mouse contain pool monocytes and neutrophils supplied not from blood but by adjacent skull vertebral bone marrow. Under pathological conditions, including spinal cord injury neuroinflammation, CNS-infiltrating myeloid can originate brain borders display transcriptional signatures distinct their blood-derived counterparts. Thus, populated marrow niches, strategically placed to supply innate under homeostatic conditions. These findings call for reinterpretation immune-cell infiltration into during autoimmunity may inform future therapeutic approaches harness meningeal cells.

Language: Английский

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Immunometabolism at the interface between macrophages and pathogens

Nature reviews. Immunology, Journal Year: 2019, Volume and Issue: 19(5), P. 291 - 304

Published: Jan. 24, 2019

Language: Английский

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Establishment and Maintenance of the Macrophage Niche

Immunity, Journal Year: 2020, Volume and Issue: 52(3), P. 434 - 451

Published: March 1, 2020

Language: Английский

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Glycolysis – a key player in the inflammatory response

FEBS Journal, Journal Year: 2020, Volume and Issue: 287(16), P. 3350 - 3369

Published: April 7, 2020

The inflammatory response involves the activation of several cell types to fight insults caused by a plethora agents, and maintain tissue homoeostasis. On one hand, cells involved in pro‐inflammatory response, such as M1 macrophages, Th1 Th17 lymphocytes or activated microglia, must rapidly provide energy fuel inflammation, which is essentially accomplished glycolysis high lactate production. other regulatory T M2 are immune regulation resolution preferentially use fatty acid oxidation through TCA cycle main source for Here, we discuss impact glycolytic metabolism at different steps response. Finally, review wide variety molecular mechanisms could explain relationship between metabolites phenotype, including signalling events, epigenetic remodelling, post‐transcriptional post‐translational modifications. Inflammatory processes common feature many age‐associated diseases, cardiovascular neurodegenerative disorders. finding that immunometabolism be master regulator inflammation broadens avenue treating inflammation‐related pathologies manipulation vascular metabolism.

Language: Английский

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Deciphering human macrophage development at single-cell resolution

Nature, Journal Year: 2020, Volume and Issue: 582(7813), P. 571 - 576

Published: May 20, 2020

Language: Английский

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The big five in fibrosis: Macrophages, myofibroblasts, matrix, mechanics, and miscommunication

Matrix Biology, Journal Year: 2018, Volume and Issue: 68-69, P. 81 - 93

Published: Feb. 1, 2018

Language: Английский

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