Integrative assessment of the transcriptome and virome of the poultry red mite Dermanyssus gallinae DOI Open Access
José M. C. Ribeiro, David J. Hartmann, Pavla Bartošová‐Sojková

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: April 21, 2022

Abstract Dermanyssus gallinae is a blood-feeding mite that parasitises on wild birds and farmed poultry. The D. has short life cycle of fewer than two weeks from the egg to an egg-laying female. remarkably swift processing blood, together with capacity blood-feed in most developmental stages, makes this highly debilitating pest. We have constructed stage-specific transcriptomes, through Illumina RNA-seq, mine repertoire protein-encoding mRNA transcripts, products which participate key processes ensure success blood digestion, rapid ontogeny, immunity. As result high reproductive capacity, prevalence egg-producing poultry farms globally causes significant economic losses. Acaricides are used limit reproduction mites target cys-loop ion channels, widely shared across phylogeny invertebrates. To catalogue comprehensive list potential invertebrate-specific we analysed additional RNA-seq library micro-dissected midguts, tissue direct exposure host anti-parasitics. phylogenetically defined groups proteins probed their sensitivity selected acaricides. Ultimately, catalogued all assembled transcripts expression values hyper-linked excel sheet available sequences individual contigs. transcriptomic data were complemented by mass-spectrometry (MS)-based metabolite identification viability assays using inhibitors applied either microinjection or artificial membrane feeding. Additionally, described RNA-virome identified novel virus dubbed Red Mite Quaranjavirus 1.

Language: Английский

Betaine lipids: Biosynthesis, functional diversity and evolutionary perspectives DOI Creative Commons

Sarah Salomon,

O Oliva,

Alberto Amato

et al.

Progress in Lipid Research, Journal Year: 2025, Volume and Issue: 97, P. 101320 - 101320

Published: Jan. 1, 2025

Language: Английский

Citations

1
Diplonemid protists possess exotic endomembrane machinery, impacting models of membrane trafficking in modern and ancient eukaryotes DOI
Kristína Záhonová, Julius Lukeš, Joel B. Dacks

et al.

Current Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

1
Recent expansion of metabolic versatility in Diplonema papillatum, the model species of a highly speciose group of marine eukaryotes DOI Creative Commons
Matus Valach, Sandrine Moreira,

Céline Petitjean

et al.

BMC Biology, Journal Year: 2023, Volume and Issue: 21(1)

Published: May 4, 2023

Abstract Background Diplonemid flagellates are among the most abundant and species-rich of known marine microeukaryotes, colonizing all habitats, depths, geographic regions world ocean. However, little is about their genomes, biology, ecological role. Results We present first nuclear genome sequence from a diplonemid, type species Diplonema papillatum . The ~ 280-Mb assembly contains 32,000 protein-coding genes, likely co-transcribed in groups up to 100. Gene clusters separated by long repetitive that include numerous transposable elements, which also reside within introns. Analysis gene-family evolution reveals last common diplonemid ancestor underwent considerable metabolic expansion. D. -specific gains carbohydrate-degradation capability were apparently acquired via horizontal gene transfer. predicted breakdown polysaccharides including pectin xylan at odds with reports peptides being predominant carbon source this organism. Secretome analysis together feeding experiments suggest predatory, able degrade cell walls live macroalgae, water plants, not only for protoplast but metabolizing cell-wall carbohydrates as an energy source. environmental barcode samples shows confined temperate coastal waters, presumably acting bioremediation eutrophication. Conclusions Nuclear information will allow systematic functional cell-biology studies It serve reference highly diverse diplonemids provide point comparison studying complement sister group Kinetoplastida, human-pathogenic taxa.

Language: Английский

Citations

21
Trypanosomes as a magnifying glass for cell and molecular biology DOI
Julius Lukeš, Dave Speijer, Alena Zı́ková

et al.

Trends in Parasitology, Journal Year: 2023, Volume and Issue: 39(11), P. 902 - 912

Published: Sept. 5, 2023

Language: Английский

Citations

19
Diplonemids – A Review on "New" Flagellates on the Oceanic Block DOI
Daria Tashyreva, Alastair G. B. Simpson, Galina Prokopchuk

et al.

Protist, Journal Year: 2022, Volume and Issue: 173(2), P. 125868 - 125868

Published: March 3, 2022

Language: Английский

Citations

23
Highly flexible metabolism of the marine euglenozoan protist Diplonema papillatum DOI Creative Commons
Ingrid Škodová‐Sveráková, Kristína Záhonová, Valéria Juricová

et al.

BMC Biology, Journal Year: 2021, Volume and Issue: 19(1)

Published: Nov. 24, 2021

The phylum Euglenozoa is a group of flagellated protists comprising the diplonemids, euglenids, symbiontids, and kinetoplastids. diplonemids are highly abundant speciose, recent tools have rendered best studied representative, Diplonema papillatum, genetically tractable. However, despite high diversity their lifestyles, ecological functions, even primary energy source mostly unknown.

Language: Английский

Citations

28
Chronic ionizing radiation might suppress resistance to pathogens in aquatic plants without substantial oxidative stress DOI
Shubhi Mishra, D. I. Gudkov, Olha Lakhneko

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 982, P. 179614 - 179614

Published: May 14, 2025

Language: Английский

Citations

0
Mitochondrion of the Trypanosoma brucei long slender bloodstream form is capable of ATP production by substrate-level phosphorylation DOI Creative Commons

Gergana Taleva,

Michaela Husová,

Brian Panicucci

et al.

PLoS Pathogens, Journal Year: 2023, Volume and Issue: 19(10), P. e1011699 - e1011699

Published: Oct. 11, 2023

The long slender bloodstream form Trypanosoma brucei maintains its essential mitochondrial membrane potential (ΔΨm) through the proton-pumping activity of F o 1 -ATP synthase operating in reverse mode. ATP that drives this hydrolytic reaction has been thought to be generated by glycolysis and imported from cytosol via an ATP/ADP carrier (AAC). Indeed, we demonstrate AAC is only can import into matrix power synthase. However, contrary expectations, deletion no effect on parasite growth, virulence or levels ΔΨ m . This suggests produced substrate-level phosphorylation pathways mitochondrion. Therefore, knocked out succinyl-CoA synthetase (SCS) gene, a key enzyme produces parasite. Its absence resulted changes metabolic landscape parasite, lowered virulence, reduced content. Strikingly, these SCS mutant parasites become more dependent as demonstrated 25-fold increase their sensitivity inhibitor, carboxyatractyloside. Since were able adapt loss culture, also analyzed immediate phenotypes manifest when expression rapidly suppressed RNAi. Importantly, performed under nutrient-limited conditions mimicking various host environments, depletion strongly affected growth In totality, data establish mitochondrion capable generating pathways.

Language: Английский

Citations

9
On the possibility of yet a third kinetochore system in the protist phylum Euglenozoa DOI Creative Commons
Corinna Benz, Maximilian W. D. Raas, Pragya Tripathi

et al.

mBio, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 30, 2024

ABSTRACT Transmission of genetic material from one generation to the next is a fundamental feature all living cells. In eukaryotes, macromolecular complex called kinetochore plays crucial roles during chromosome segregation by linking chromosomes spindle microtubules. Little known about this process in evolutionarily diverse protists. Within supergroup Discoba, Euglenozoa forms speciose group unicellular flagellates—kinetoplastids, euglenids, and diplonemids. Kinetoplastids have an unconventional system, while euglenids subunits that are conserved among most eukaryotes. For diplonemids, extremely abundant marine flagellates, it remains unclear what kind kinetochores present. Here, we employed deep homology detection protocols using profile-versus-profile Hidden Markov Model searches AlphaFold-based structural comparisons detect homologies might been previously missed. Interestingly, still could not orthologs for kinetoplastid or canonical with few exceptions including putative centromere-specific histone H3 variant (cenH3/CENP-A), checkpoint protein Mad2, chromosomal passenger members Aurora INCENP, broadly proteins like CLK kinase meiotic synaptonemal SYCP2/3 also function at kinetochores. We examined localization five candidate kinetochore-associated model diplonemid, Paradiplonema papillatum. Pp CENP-A shows discrete dots nucleus, implying likely component. KKT10/19 , SYCP2L1 KKT17/18 INCENP reside but no clear was observed. Altogether, these results point possibility diplonemids evolved hitherto unknown type system. IMPORTANCE A assembly essential eukaryotic cell division. Therefore, characterization across species understanding mechanisms involved key tree life. particular, little divergent protists such as Euglenozoa, flagellates includes kinetoplastids, latter being highly component plankton. While kinetoplastids system similar traditional preliminary detected neither nor components state-of-the-art bulk kinetoplastid-specific except variant. Our suggest do resemble ones.

Language: Английский

Citations

3
A mixture of innate cryoprotectants is key for freeze tolerance and cryopreservation of a drosophilid fly larva DOI Open Access
Lukáš Kučera, Martin Moos, Tomáš Štětina

et al.

Journal of Experimental Biology, Journal Year: 2022, Volume and Issue: 225(8)

Published: April 5, 2022

ABSTRACT Insects that naturally tolerate internal freezing produce complex mixtures of multiple cryoprotectants (CPs). Better knowledge on composition these mixtures, and the mechanisms individual CP interactions, could inspire development laboratory formulations optimized for cryopreservation cells other biological material. Here, we identify quantify (using high resolution mass spectrometry) a range putative CPs in larval tissues subarctic fly, Chymomyza costata, which survives long-term liquid nitrogen. The proline, trehalose, glutamine, asparagine, glycine betaine, glycerophosphoethanolamine, glycerophosphocholine sarcosine accumulate hemolymph ratio 313:108:55:26:6:4:2.9:0.5 mmol l−1. Using calorimetry, show artificial mimicking concentrations major freeze-tolerant larvae, suppress melting point water significantly reduce ice fraction. We demonstrate bioassay administered through diet act synergistically rather than additively to enable otherwise freeze-sensitive larvae. matrix-assisted laser desorption/ionization spectrometry imaging (MALDI-MSI), during slow extracellular trehalose becomes concentrated partially dehydrated where it stimulates transition amorphous glass phase. In contrast, proline moves boundary between probably forms layer dense viscoelastic liquid. propose liquids may protect macromolecules from thermomechanical shocks associated with transfer into out

Language: Английский

Citations

13