Insect cell plasma membranes do, while soluble enzymes do not, need stabilization by accumulated cryoprotectant molecules during freezing stress DOI Creative Commons
Robert Grgac, Jan Rozsypal, Lauren E. Des Marteaux

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: June 26, 2022

Abstract Ability to survive freezing of extracellular body fluids evolved in several species vertebrate ectotherms, many plants, and occurs relatively often freeze-tolerant insects. Most the multicellular organisms, however, are freeze-sensitive. Here we test coupled hypotheses postulating that: (i) irreversible denaturation proteins loss integrity biological membranes two ultimate molecular mechanisms injury freeze-sensitive insects; (ii) seasonally accumulated small cryoprotective molecules (CPs) protect against We show that seven different enzymes exhibit no or only partial activity upon lethal stress applied vivo whole larva drosophilid fly, Chymomyza costata . In contrast, lost when extracted frozen vitro a diluted buffer solution. This was fully prevented by adding low concentrations wide array compounds including C. native CPs, other metabolites, bovine serum albumin (BSA), even biologically inert artificial Histodenz Ficoll. Next, plasma fat cells lose but not larvae. Freezing , resulted membrane both Different additives showed widely capacities (from none high) added medium. A complete rescue observed for mixture proline, trehalose BSA. Significance statement suggest insect soluble primary targets injury. They inactivated insects exposed as they sufficiently protected from complex composition solutions. Next cell likely The stress, while their is molecules, also proteins,

Language: Английский

Cryoprotective Metabolites Are Sourced from Both External Diet and Internal Macromolecular Reserves during Metabolic Reprogramming for Freeze Tolerance in Drosophilid Fly, Chymomyza costata DOI Creative Commons
Martin Moos,

Jaroslava Korbelová,

Tomáš Štětina

et al.

Metabolites, Journal Year: 2022, Volume and Issue: 12(2), P. 163 - 163

Published: Feb. 9, 2022

Many cold-acclimated insects accumulate high concentrations of low molecular weight cryoprotectants (CPs) in order to tolerate subzero temperatures or internal freezing. The sources from which carbon skeletons for CP biosynthesis are driven, and the metabolic reprogramming linked cold acclimation, not sufficiently understood. Here we aim resolve metabolism putative CPs by mapping relative changes concentration 56 metabolites expression 95 relevant genes as larvae drosophilid fly, Chymomyza costata transition a freeze sensitive tolerant phenotype during gradual acclimation. We found that C. may directly assimilate amino acids proline glutamate diet acquire at least half their large stocks (up 55 µg per average 2 mg larva). Metabolic conversion glutamine reserves build up early diapause explain second accumulation, while ornithine degradation larval collagens other proteins might be two additional minor sources. Next, confirm glycogen represent major source glucose units trehalose synthesis accumulation 27 larva), serve an source. Finally, suggest interconversions phospholipids release accumulated glycero-phosphocholine (GPC) -ethanolamine (GPE). Choline is methylamines: glycine-betaine sarcosine. sum methylamines together with GPE GPC represents approximately larva. In conclusion, food ingestion important direct assimilation of, and/or conversions to, diapausing insect. So far, cold-acclimation- was considered sourced mainly macromolecular reserves.

Language: Английский

Citations

20
Expanding the cryoprotectant toolbox in biomedicine by multifunctional antifreeze peptides DOI
Shiva Hemmati

Biotechnology Advances, Journal Year: 2025, Volume and Issue: unknown, P. 108545 - 108545

Published: Feb. 1, 2025

Language: Английский

Citations

0
Stabilization of insect cell membranes and soluble enzymes by accumulated cryoprotectants during freezing stress DOI Creative Commons
Robert Grgac, Jan Rozsypal, Lauren E. Des Marteaux

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(41)

Published: Oct. 3, 2022

Most multicellular organisms are freeze sensitive, but the ability to survive freezing of extracellular fluids evolved in several vertebrate ectotherms, some plants, and many insects. Here, we test coupled hypotheses that perpetuated literature: irreversible denaturation proteins loss biological membrane integrity two ultimate molecular mechanisms injury freeze-sensitive insects seasonally accumulated small cryoprotective molecules (CPs) stabilize membranes against freeze-tolerant Using drosophilid fly, Chymomyza costata, show seven different soluble enzymes exhibit no or only partial activity upon lethal stress applied vivo whole larvae. In contrast, lost when extracted frozen vitro a diluted buffer solution. This was fully prevented by adding low concentrations wide array compounds buffer, including C. costata native CPs, other metabolites, bovine serum albumin (BSA), even biologically inert artificial HistoDenz Ficoll. Next, fat body plasma lose not Freezing cells vitro, however, resulted both Different additives showed widely capacities protect added media. A complete rescue larvae observed with mixture proline, trehalose, BSA.

Language: Английский

Citations

16
Insect cross-tolerance to freezing and drought stress: role of metabolic rearrangement DOI
Petr Hůla, Martin Moos, Lauren E. Des Marteaux

et al.

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2022, Volume and Issue: 289(1976)

Published: June 8, 2022

The accumulation of trehalose has been suggested as a mechanism underlying insect cross-tolerance to cold/freezing and drought. Here we show that exposing diapausing larvae the drosophilid fly, Chymomyza costata dry conditions significantly stimulates their freeze tolerance. It does not, however, improve tolerance desiccation, nor it affect concentrations. Next, use metabolomics compare complex alterations intermediary metabolism pathways in response three environmental factors with different ecological meanings: drought (an stressor causing mortality), decreasing ambient temperatures acclimation stimulus for improvement cold hardiness), short days signal inducing diapause). We all trigger qualitatively similar metabolic rearrangement phenotypic outcome—improved larval similarities include (but are not restricted to) typical compatible solutes energy-rich molecules (phosphagens). Based on these results, suggest transition suppression (a state which chemical energy demand is relatively low but need stabilization macromolecules high) represents common axis pathway reorganization towards non-toxic cytoprotective compounds, turn

Language: Английский

Citations

13
Metabolomics as a tool to elucidate biochemical cold adaptation in insects DOI Creative Commons
Scott A. L. Hayward, Hervé Colinet

Current Opinion in Insect Science, Journal Year: 2023, Volume and Issue: 58, P. 101061 - 101061

Published: May 25, 2023

Language: Английский

Citations

8
How to eliminate pathogen without killing oneself? Immunometabolism of encapsulation and melanization in Drosophila DOI Creative Commons
Tomáš Doležal

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Dec. 6, 2023

Cellular encapsulation associated with melanization is a crucial component of the immune response in insects, particularly against larger pathogens. The infection Drosophila larva by parasitoid wasps, like Leptopilina boulardi , most extensively studied example. In this case, and embryo linked to activation plasmatocytes that attach surface parasitoid. Additionally, differentiation lamellocytes encapsulate parasitoid, along crystal cells, accountable for process. Encapsulation lead production toxic molecules are concentrated capsule around and, at same time, protect host from response. Thus, cellular represent primarily metabolic process involving metabolism cell differentiation, radicals, but also melanin antioxidants. As such, it has significant implications physiology systemic metabolism. Proper regulation within as well level entire organism, therefore essential an efficient impacts health overall fitness organism survives. purpose “perspective” article map what we know about type response, place context possible physiology, highlight open questions related important insect

Language: Английский

Citations

5
Extracellular freezing induces a permeability transition in the inner membrane of muscle mitochondria of freeze-sensitive but not freeze-tolerant Chymomyza costata larvae DOI Creative Commons
Tomáš Štětina, Vladimı́r Košťál

Frontiers in Physiology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 7, 2024

Background: Many insect species have evolved the ability to survive extracellular freezing. The search for underlying principles of their natural freeze tolerance remains hampered by our poor understanding mechanistic nature freezing damage itself. Objectives: Here, in potential primary cellular targets damage, we compared mitochondrial responses (changes morphology and physical integrity, respiratory chain protein functionality, inner membrane (IMM) permeability) freeze-sensitive vs. freeze-tolerant phenotypes larvae drosophilid fly, Chymomyza costata. Methods: Larvae were exposed stress at -30°C 1 h, which is invariably lethal phenotype but readily survived phenotype. Immediately after melting, metabolic activity muscle cells was assessed Alamar Blue assay, mitochondria examined transmission electron microscopy, functionality oxidative phosphorylation system measured Oxygraph-2K microrespirometry. Results: remained morphologically functionally intact stress. In contrast, most swollen, matrix diluted enlarged volume, structure IMM cristae lost. Despite this morphological transfer proteins partially functional lethally frozen larvae, still exhibiting strong specific substrates transferring electrons oxygen. However, coupling ATP synthesis severely impaired. Based on these results, formulated a hypothesis linking observed swelling sudden loss barrier function IMM.

Language: Английский

Citations

1
Effects of cryopreservation in the presence of Natural Deep Eutectic Solvents (NADESs) on sperm parameters DOI

Haniyeh Saadat Maryan,

Fatemeh Ghasemian, Mohammad Hadi Bahadori

et al.

Cryobiology, Journal Year: 2023, Volume and Issue: 112, P. 104550 - 104550

Published: May 23, 2023

Language: Английский

Citations

3
Lipid Properties and Metabolism in Response to Cold DOI
Thomas Enriquez, Nicholas M. Teets

Advances in experimental medicine and biology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Citations

0
REVIEW OF MODERN METHODS OF CRYOPRESERVATION OF VARIOUS TYPES OF BIOLOGICAL MATERIAL DOI Open Access

Е. В. Заикина,

A.S. Goncharova,

V.V. Pozdnyakova

et al.

Современные проблемы науки и образования (Modern Problems of Science and Education), Journal Year: 2022, Volume and Issue: №4 2022, P. 2 - 2

Published: Jan. 1, 2022

УДК 601.2 ОБЗОР СОВРЕМЕННЫХ МЕТОДОВ КРИОКОНСЕРВАЦИИ РАЗЛИЧНЫХ ВИДОВ БИОЛОГИЧЕСКОГО МАТЕРИАЛА Заикина Е.В. 1 , Гончарова А.С. Позднякова В.В. Пандова О.В. Пржедецкий Ю.В. Воловик В.Г. 2 Карасев Т.С

Language: Русский

Citations

1