Integrative assessment of the transcriptome and virome of the poultry red mite Dermanyssus gallinae DOI Open Access
José M. C. Ribeiro, David J. Hartmann, Pavla Bartošová‐Sojková

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: April 21, 2022

Abstract Dermanyssus gallinae is a blood-feeding mite that parasitises on wild birds and farmed poultry. The D. has short life cycle of fewer than two weeks from the egg to an egg-laying female. remarkably swift processing blood, together with capacity blood-feed in most developmental stages, makes this highly debilitating pest. We have constructed stage-specific transcriptomes, through Illumina RNA-seq, mine repertoire protein-encoding mRNA transcripts, products which participate key processes ensure success blood digestion, rapid ontogeny, immunity. As result high reproductive capacity, prevalence egg-producing poultry farms globally causes significant economic losses. Acaricides are used limit reproduction mites target cys-loop ion channels, widely shared across phylogeny invertebrates. To catalogue comprehensive list potential invertebrate-specific we analysed additional RNA-seq library micro-dissected midguts, tissue direct exposure host anti-parasitics. phylogenetically defined groups proteins probed their sensitivity selected acaricides. Ultimately, catalogued all assembled transcripts expression values hyper-linked excel sheet available sequences individual contigs. transcriptomic data were complemented by mass-spectrometry (MS)-based metabolite identification viability assays using inhibitors applied either microinjection or artificial membrane feeding. Additionally, described RNA-virome identified novel virus dubbed Red Mite Quaranjavirus 1.

Language: Английский

Blood-feeding adaptations and virome assessment of the poultry red mite Dermanyssus gallinae guided by RNA-seq DOI Creative Commons
José M. C. Ribeiro, David J. Hartmann, Pavla Bartošová‐Sojková

et al.

Communications Biology, Journal Year: 2023, Volume and Issue: 6(1)

Published: May 13, 2023

Dermanyssus gallinae is a blood-feeding mite that parasitises wild birds and farmed poultry. Its remarkably swift processing of blood, together with the capacity to blood-feed during most developmental stages, makes this highly debilitating pest. To identify specific adaptations digestion haemoglobin-rich diet, we constructed compared transcriptomes from starved blood-fed stages parasite identified midgut-enriched transcripts. We noted midgut transcripts encoding cysteine proteases were upregulated blood meal. Mapping full proteolytic apparatus, reduction in suite proteases, missing homologues for Cathepsin B C. have further phylogenetically analysed three distinct vitellogenins facilitate reproductive mites. also fully mapped haem biosynthesis ferritin-based system iron storage inter-tissue trafficking. Additionally, proteins implicated immune signalling (Toll IMD pathways) activity (defensins thioester-containing proteins), RNAi, ion channelling (with targets commercial acaricides such as Fluralaner, Fipronil, Ivermectin). Viral sequences filtered Illumina reads described, part, RNA-virome D. identification novel virus, Red quaranjavirus 1.

Language: Английский

Citations

8
The Porifera microeukaryome: Addressing the neglected associations between sponges and protists DOI Creative Commons
Gabriel Nascimento-Silva, Cristiane Cassiolato Pires Hardoim, Márcio Reis Custódio

et al.

Microbiological Research, Journal Year: 2022, Volume and Issue: 265, P. 127210 - 127210

Published: Sept. 27, 2022

Language: Английский

Citations

9
Delineating transitions during the evolution of specialised peroxisomes: Glycosome formation in kinetoplastid and diplonemid protists DOI Creative Commons
Diego Andrade-Alviárez, Alejandro David Bonive-Boscan, Ana J. Cáceres

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 10

Published: Sept. 12, 2022

One peculiarity of protists belonging to classes Kinetoplastea and Diplonemea within the phylum Euglenozoa is compartmentalisation most glycolytic enzymes peroxisomes that are hence called glycosomes. This pathway not sequestered in third Euglenozoan class, Euglenida. Previous analysis well-studied kinetoplastids, ‘TriTryps’ parasites Trypanosoma brucei , cruzi Leishmania spp., identified glycosomes other metabolic processes usually present peroxisomes. In addition, trypanosomatid peroxins, i.e. proteins involved biogenesis these organelles, divergent from human yeast orthologues. recent years, genomes, transcriptomes proteomes for a variety euglenozoans have become available. Here, we track possible evolution by querying databases, as well genome Naegleria gruberi non-euglenozoan, which belongs same protist supergroup Discoba. We searched orthologues TriTryps glycosomal metabolism biogenesis. Predicted cellular location(s) each enzyme was inferred presence or absence peroxisomal-targeting signals. Combined with survey relevant literature, refine extensively our previously postulated hypothesis about glycosome evolution. The data agree glycolysis compartmentalised common ancestor kinetoplastids diplonemids, yet additionally indicates found extant trypanosomatids, but eukaryotes were either this shortly after separation two lineages. contrast, peroxin divergence evident all euglenozoans. Following their gain complexity, subsequent peroxisome/glycosome function complex. hypothesize enzymes, cofactors subsequently provided selective advantage diplonemids during changing marine environments. contend specific properties derived ancestral key: existence nonselective pores small solutes possibility high turnover pexophagy. Critically, such pexophagy characterised trypanosomatids. Increasing amenability free-living recently isolated experimental study means interpretation bioinformatic suited interrogation.

Language: Английский

Citations

7
How mitochondria showcase evolutionary mechanisms and the importance of oxygen DOI Creative Commons
Dave Speijer

BioEssays, Journal Year: 2023, Volume and Issue: 45(6)

Published: March 25, 2023

Abstract Darwinian evolution can be simply stated: natural selection of inherited variations increasing differential reproduction. However, formulated thus, links with biochemistry, cell biology, ecology, and population dynamics remain unclear. To understand interactive contributions chance selection, higher levels biological organization (e.g., endosymbiosis), complexities competing forces, emerging novelties (such as eukaryotes or meiotic sex), we must analyze actual examples. Focusing on mitochondria, I will illuminate how biology makes sense life's evolution, the concepts involved. First, looking at bacterium – mitochondrion transition: merging an archaeon, it lost its independence, but played a decisive role in eukaryogenesis, extremely efficient aerobic ATP generator internal ROS source. Second, surveying later adaptations diversifications illustrates such constructive neutral dynamic interactions between endosymbionts hosts, contingency life histories, metabolic reprogramming. Without oxygen, mitochondria disappear; (intermittent) oxygen diversification occurs highly complex ways, especially upon (temporary) phototrophic substrate supply. These expositions show model to fruitful paradigm.

Language: Английский

Citations

4
Compartmentalization of galactan biosynthesis in mycobacteria DOI Creative Commons
Karin Savková, Maksym Danchenko, Viktória Fabianová

et al.

Journal of Biological Chemistry, Journal Year: 2024, Volume and Issue: 300(3), P. 105768 - 105768

Published: Feb. 16, 2024

Galactan polymer is a prominent component of the mycobacterial cell wall core. Its biogenesis starts at cytoplasmic side plasma membrane by build-up linker disaccharide [rhamnosyl (Rha) - N-acetyl-glucosaminyl (GlcNAc) phosphate] on decaprenyl-phosphate carrier. This decaprenyl-P-P-GlcNAc-Rha intermediate extended two bifunctional galactosyl transferases, GlfT1 and GlfT2, then it translocated to periplasmic space an ABC transporter Wzm-Wzt. The core synthesis finalized action array arabinosyl mycolyl ligases that catalyze attachment arabinogalactan peptidoglycan through region. Based visualization GlfT2 enzyme fused with fluorescent tags was proposed galactan polymerization takes place in specific compartment envelope, intracellular domain, representing pure free components (previously denoted as "PMf" domain), which localizes polar region mycobacteria. In this work, we examined activity galactan-producing cellular machine cell-wall containing envelope fraction wall-free prepared from Mycobacterium smegmatis assays using radioactively labeled substrate UDP-[

Language: Английский

Citations

1
Physiology of Overwintering in a Microhabitat Fully Exposed to Adverse Weather Conditions: Lime Seed Bugs on Tree Trunks and Branches DOI
Jan Rozsypal, Martin Moos, Petr Vodrážka

et al.

ZOOLOGICAL SCIENCE, Journal Year: 2024, Volume and Issue: 42(1)

Published: Nov. 19, 2024

The present study investigates the physiological aspects of overwintering in an exposed microhabitat lime seed bug, Oxycarenus lavaterae. We found that bugs do not survive freezing their body fluids, but instead rely on supercooling (freeze avoidance). seasonal modulation capacity was very limited, with midwinter mean point reaching -15.5°C, individual variability high (- 6°C to - 22°C). Most other parameters (utilization energy substrates, changes hydration, and metabolite composition [although levels were low]) consistent general knowledge gathered for freeze-avoiding insects. A significant exception amount osmotically active water ("freezable" water), which constituted up 95% bug water. Such a proportion is unusually high, as it typically ranges from 59% 86% insects invertebrates. At present, we have no plausible explanation this anomaly or its possible relationship bug's microhabitat.

Language: Английский

Citations

1
Deep Insights into the Specific Evolution of Fungal Hybrid B Heme Peroxidases DOI Creative Commons
Marcel Zámocký, Miloš Musil, Maksym Danchenko

et al.

Biology, Journal Year: 2022, Volume and Issue: 11(3), P. 459 - 459

Published: March 17, 2022

In this study, we focus on a detailed bioinformatics analysis of hyBpox genes, mainly within the genomes Sclerotiniaceae (Ascomycota, Leotiomycetes), which is specifically evolved fungal family necrotrophic host generalists and saprophytic or biotrophic specialists. Members genus Sclerotium produce only sclerotia no fruiting bodies spores. Thus, their physiological role for peroxidases remains open. A representative species, S. cepivorum, dangerous plant pathogen causing white rot in Allium particularly onions, leeks, garlic. On worldwide basis, caused by soil-borne fungus apparently most serious threat to Allium-crop production. We have also found very similar peroxidase sequences related sclerotiorum, although with minor yet important modifications architecture its active centre. The presence ScephyBpox1-specific mRNA was confirmed transcriptomic analysis. Hybrid B at protein level as sole extracellular secretome cepivorum through proteomic analyses. This prompted us systematically search all available genes coding heme whole Sclerotiniaceae. present here reconstruction molecular phylogeny analyse unique aspects conserved-sequence features structural folds corresponding ancestral sequences.

Language: Английский

Citations

4
Two canonically aerobic foraminifera express distinct peroxisomal and mitochondrial metabolisms DOI Creative Commons
Christopher Powers, Fatma Gomaa,

Elizabeth B. Billings

et al.

Frontiers in Marine Science, Journal Year: 2022, Volume and Issue: 9

Published: Dec. 2, 2022

Certain benthic foraminifera thrive in marine sediments with low or undetectable oxygen. Potential survival avenues used by these supposedly aerobic protists include fermentation and anaerobic respiration, although details on their adaptive mechanisms remain elusive. To better understand the metabolic versatility of foraminifera, we studied two species that oxygen-depleted sediments. Here detail, via transcriptomics metatranscriptomics, differential gene expression Nonionella stella Bolivina argentea , collected from Santa Barbara Basin, California, USA, response to varied oxygenation chemical amendments. Organelle-specific reconstructions revealed utilize adaptable mitochondrial peroxisomal metabolism. N. most abundant anoxia characterized lack food vacuoles abundance intracellular lipid droplets, was predicted couple putative beta-oxidation glyoxylate cycle a versatile electron transport system partial TCA cycle. In contrast, B. hypoxia contains vacuoles, gluconeogenesis full but lacks key genes. These adaptations likely confer ecological success while encountering deoxygenation expand our understanding modifications interactions between mitochondria peroxisomes protists.

Language: Английский

Citations

4
Subunit composition of mitochondrial dehydrogenase complexes in diplonemid flagellates DOI Creative Commons
Kristína Záhonová, Matus Valach, Pragya Tripathi

et al.

Biochimica et Biophysica Acta (BBA) - General Subjects, Journal Year: 2023, Volume and Issue: 1867(9), P. 130419 - 130419

Published: July 13, 2023

In eukaryotes, pyruvate, a key metabolite produced by glycolysis, is converted tripartite mitochondrial pyruvate dehydrogenase (PDH) complex to acetyl-coenzyme A, which fed into the tricarboxylic acid cycle. Two additional enzyme complexes with analogous composition catalyze similar oxidative decarboxylation reactions albeit using different substrates, branched-chain ketoacid (BCKDH) and 2-oxoglutarate (OGDH) complex. Comparative transcriptome analyses of diplonemids, one most abundant diverse groups oceanic protists, indicate that conventional E1, E2, E3 subunits PDH are lacking. E1 was apparently replaced in euglenozoan ancestor diplonemids an AceE protein archaeal type, substitution we also document dinoflagellates. Here, demonstrate mitochondrion model diplonemid Paradiplonema papillatum displays activities. Protein mass spectrometry mitochondria reveal as subunit BCKDH. This corroborates view functional component We hypothesize acquiring AceE, not only lost eukaryotic-type but E2 complex, present other euglenozoans. posit activity seems be carried out partners from BCKDH and/or OGDH.

Language: Английский

Citations

1
Comparative analysis of mitochondrion-related organelles in anaerobic amoebozoans DOI Creative Commons
Kristína Záhonová, Zoltán Füssy, Courtney W. Stairs

et al.

Microbial Genomics, Journal Year: 2023, Volume and Issue: 9(11)

Published: Nov. 23, 2023

Archamoebae comprises free-living or endobiotic amoebiform protists that inhabit anaerobic microaerophilic environments and possess mitochondrion-related organelles (MROs) adapted to function anaerobically. We compared in silico reconstructed MRO proteomes of eight species (six genera) found the common ancestor possessed very few typical components protein translocation machinery, electron transport chain tricarboxylic acid cycle. On other hand, it contained a sulphate activation pathway bacterial iron–sulphur (Fe-S) assembly system MIS-type. The metabolic capacity MROs, however, varies markedly within this clade. glycine cleavage is widely conserved among Archamoebae, except Entamoeba , probably owing its role catabolic one-carbon metabolism. MRO-based pyruvate metabolism was dispensed subgroups Entamoebidae Rhizomastixidae, whereas could have been lost isolated cases Rhizomastix libera Mastigamoeba abducta Endolimax sp. MIS duplicated Mastigamoebidae Pelomyxidae, one copies took over Fe-S their MRO. In we hypothesize cluster both compartments may be facilitated by dual localization single system. not find evidence for changes functions response habitat; appears such environmental drivers do strongly affect reduction group eukaryotes.

Language: Английский

Citations

1