Circulating miR-29c, miR-195, and miR-486 Are Objective Indicators to Determine the Moderate Intensity of Resistance Exercise DOI Open Access

Daisuke Takamura,

K. Iwata,

Shota Inoue

et al.

Cureus, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 22, 2024

Background and objective Moderate exercise is important for health; however, there are variations among individuals in terms of characterizing moderate intensity it difficult to identify. In light this, the purpose this study was identify new indicators determine effective intensity. Methods This involved both human animal experiments. After subjecting mice intensity, microarray analysis circulating microRNA expression conducted candidates Then, we assessed if these microRNAs were altered after aerobic or resistance exercises humans using quantitative real-time PCR. Twelve healthy males randomly assigned two groups: low-intensity group (LI group) high-intensity (HI they underwent four weeks program. Results Based on analysis, 188 exercise, 167 exercise. Combining findings with data from some published reports, selected miR-29c, miR-23b, miR-222, miR-195, miR-126, miR-133a, miR-486 as biomarkers study, physical performance improved only HI group. Of microRNAs, increased immediately Fold change correlated changes knee extensor strength (r=0.744, p=0.005). Conclusions Resistance at upregulated miR-486. Hence, may serve Among them, aid predicting response

Language: Английский

The Impact of the Competition on miRNA, Proteins, and Metabolites in the Blood Exosomes of the Yili Horse DOI Open Access

Yuan Xue,

Xinkui Yao,

Yaqi Zeng

et al.

Genes, Journal Year: 2025, Volume and Issue: 16(2), P. 224 - 224

Published: Feb. 15, 2025

Horse racing may cause stress-induced physiological changes and tissue damage in horses, but the miRNA expression, protein metabolic substances plasma exosomes of Yili horse after are still unclear. This study detected miRNA, horses before competition, providing new insights for post-race recovery care horses. Eight three-year-old that had undergone training were selected as research subjects, with four not competed control group participated competition half an hour group. Extract whole blood separate from two groups then extract exosomes; MiRNAs, proteins, metabolites extracellular vesicles analyzed using miRNAomics, proteomics, metabolomics. P Result: After levels miRNAs related to cytoplasm nucleus increased, transcription transcriptional regulation biological processes significantly increased. The proteins also cell signaling function carbohydrates their reduced. process causes significant metabolomics which mainly regulation.

Language: Английский

Citations

0
MicroRNA in cardiometabolic health and disease: The perspectives of sex, gender and personalised medicine DOI
Stefano Ministrini, Teresa Padró

European Journal of Clinical Investigation, Journal Year: 2024, Volume and Issue: 54(7)

Published: April 16, 2024

Abstract Background Personalized medicine represents a novel and integrative approach that focuses on an individual's genetics epigenetics, precision medicine, lifestyle exposures as key players of health status disease phenotypes. Methods In this narrative review, we aim to carefully discuss the current knowledge gender disparities in cardiometabolic diseases, consider sex‐ specific expression miRNAs their role promising tool medicine. Results Personalised overcomes restricted care patient based binomial sex approach, by enriching itself with holistic dynamic integration. Recognized major worldwide emergency, disorders continue rise, impacting systems requiring more effective targeted strategies. Several drivers might affect onset progression males females at multiple levels. respect, distinct contribution genetic epigenetic mechanisms, molecular physiological pathways, hormones, visceral fat subcutaneous lead differences burden outcomes females. Conclusions Sex play pivotal because influence physiology each individual way they interact environment from intrauterine life.

Language: Английский

Citations

3
MicroRNAs als molekulare Marker für physiologische Anpassungsreaktionen auf körperliche Ausdauerbelastung DOI Creative Commons
Ralf Schubert,

J. B. Wagner,

N. Kämpfer

et al.

Zentralblatt für Arbeitsmedizin Arbeitsschutz und Ergonomie, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 9, 2024

Zusammenfassung In den vergangenen Jahren hat sich die Forschung verstärkt der Identifizierung von MicroRNAs ( miRNAs ) als Biomarker gewidmet. Auch physiologischen Anpassungen an körperliche Ausdauerbelastungen wurden in diesem Kontext untersucht. sind kleine nichtkodierende RNAs, eine Schlüsselrolle bei posttranskriptionellen Genregulation spielen. Diese kurze Übersichtsarbeit gibt einen Überblick über Grundlagen , ihre biogenetischen Wege und Funktionen sowie aktuelle Erkenntnisse Rolle Anpassung Ausdauertraining. Die zeigt, dass im Blut anderen Körperflüssigkeiten zirkulieren, Reaktion auf sportliche Betätigung spezifischen Mustern exprimiert werden. Veränderungen abhängig Art (z. B. anaerobe oder aerobe Belastung, Ausdauer- vs. Krafttraining), Intensität Dauer des Trainings. Darüber hinaus es Hinweise darauf, geschlechtsspezifische Reaktionen Belastungen zeigen. bieten nicht nur Einblicke Mechanismen Trainingsanpassung, sondern könnten auch praktische Anwendungen präventiven Gesundheitsförderung betrieblichen Gesundheitsmanagement finden. Langzeitstudien weitere jedoch erforderlich, um genauen Zusammenhänge das Potenzial therapeutische Ziele vollständig zu verstehen.

Citations

0
Circulating miR-29c, miR-195, and miR-486 are objective indicators to determine the moderate intensity of resistance exercise DOI Creative Commons

Daisuke Takamura,

K. Iwata,

Shota Inoue

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 18, 2024

Abstract Purpose Moderate exercise is important for health; however, individuals differ in moderate intensity and it difficult to identify. The purpose of this study was identify new objective indicators determine effective intensity. Methods After the mice were exercised intensity, microarray analysis circulating microRNA expression conducted candidates Then, we assessed if these microRNAs altered after aerobic or resistance exercises human using quantitative real-time PCR. Twelve healthy males randomly assigned 2 groups: low-intensity group (LI group) high-intensity (HI undertook 4 weeks program. Results As a result analysis, 188 changed exercise, 167 exercise. Combined with some published reports, selected miR-29c, miR-23b, miR-222, miR-195, miR-126, miR-133a, miR-486 as biomarkers In study, physical performance improved only HI group. Of microRNAs, increased immediately Fold change correlated changes knee extensor strength (r = 0.744, P 0.005). Conclusion Resistance at upregulated miR-486. These may serve Among them, aid predicting response

Language: Английский

Citations

0
The transcriptomic signature of age and sex is not conserved in human primary myocytes DOI Open Access
Séverine Lamon, Megan Soria, Ross D. Williams

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 18, 2024

Abstract Human primary muscle cell (HPMC) lines derived from skeletal biopsies are potentially powerful tools to interrogate the molecular pathways underlying fundamental mechanisms. HPMCs retain their genome in culture, but many endogenous circulating factors not present vitro environment or at concentrations that do mirror physiological levels. To address assumption valid models of age and sex-specificity human research, we examined what extent HPMC maintain source phenotypes culture. A comparison transcriptomic signature 30 (N = 10 young males, N females, old males) corresponding indicated a near-complete lack retention genes were differentially regulated vivo when compared equivalent, except for some encoded on Y-chromosome. The diversity population sex- age-dependent cellular milieu likely contribute these observations, which call caution using as an experimental model sex age. Nuclear single sequencing HMPCs grown 3D culture organoids under strain might therefore provide closer alternative tissue.

Language: Английский

Citations

0
Circulating miR-29c, miR-195, and miR-486 Are Objective Indicators to Determine the Moderate Intensity of Resistance Exercise DOI Open Access

Daisuke Takamura,

K. Iwata,

Shota Inoue

et al.

Cureus, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 22, 2024

Background and objective Moderate exercise is important for health; however, there are variations among individuals in terms of characterizing moderate intensity it difficult to identify. In light this, the purpose this study was identify new indicators determine effective intensity. Methods This involved both human animal experiments. After subjecting mice intensity, microarray analysis circulating microRNA expression conducted candidates Then, we assessed if these microRNAs were altered after aerobic or resistance exercises humans using quantitative real-time PCR. Twelve healthy males randomly assigned two groups: low-intensity group (LI group) high-intensity (HI they underwent four weeks program. Results Based on analysis, 188 exercise, 167 exercise. Combining findings with data from some published reports, selected miR-29c, miR-23b, miR-222, miR-195, miR-126, miR-133a, miR-486 as biomarkers study, physical performance improved only HI group. Of microRNAs, increased immediately Fold change correlated changes knee extensor strength (r=0.744, p=0.005). Conclusions Resistance at upregulated miR-486. Hence, may serve Among them, aid predicting response

Language: Английский

Citations

0