Current Microbiology, Journal Year: 2025, Volume and Issue: 82(2)
Published: Jan. 3, 2025
Language: Английский
The Microbe, Journal Year: 2024, Volume and Issue: 4, P. 100131 - 100131
Published: July 20, 2024
We present the review and meta-analysis of prevalence Staphylococcus aureus isolates from various food sources. PubMed, Google Scholar, Scopus, Science Direct were searched for articles 2012 to 2022. S. is a pathogenic bacterium in natural skin flora, that can cause variety diseases with different degrees severity. Although its habitat are humans animals, be found water, soil contaminated surfaces. Consequently, multiple routes involved contamination by aureus. The was most prevalent ready-to-eat (35.1 %), meat (21.7 %) dairy products (18.5 %). Among products, represented 59.51 % distributed as such: 44 beef meat, 28 pork 22 chicken 6 turkey meat. Antibiotic resistance studies showed penicillins common (61 while resistant quinolones, amphenicols rifamycins low (<10 Pooled antimicrobial isolated strains revealed 68 all carried at least one antibiotic clinical use. Future needed assess resistance, food-associated stress biofilm formation foodborne pathogen Furthermore, improved diagnostic tools implementation surveillance programs could lead reduction burden caused
Language: Английский
Citations
6
Microbiological Research, Journal Year: 2023, Volume and Issue: 271, P. 127345 - 127345
Published: March 1, 2023
Language: Английский
Citations
16
Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 13
Published: Jan. 6, 2023
Rapid and accurate diagnosis of causative pathogens in mastitis would minimize the imprudent use antibiotics and, therefore, reduce spread antimicrobial resistance. Whole genome sequencing offers a unique opportunity to study microbial community resistance (AMR) mastitis. However, complexity milk samples presence high amount host DNA from infected udders often make this very challenging.Here, we tested 24 bovine (18 six non-mastitis) using four different commercial kits (Qiagens' DNeasy® PowerFood® Microbial, Norgens' Milk Bacterial Isolation, Molzyms' MolYsis™ Plus Complete5) combination with filtration, low-speed centrifugation, nuclease, 10% bile extract male (Ox bile). Isolated was quantified, checked for presence/absence pathogen PCR sequenced MinION nanopore sequencing. Bioinformatics analysis performed taxonomic classification gene detection.The results showed that designed explicitly bacterial isolation food dairy matrices could not deplete/minimize DNA. Following Complete 5 + Ox micrococcal nuclease combination, on average, 17% 66.5% reads were classified as Staphylococcus aureus reads, respectively. This also effectively enriched other pathogens, including Escherichia coli Streptococcus dysgalactiae. Furthermore, approach, identified important AMR genes such Tet (A), (38), fosB-Saur, blaZ. We even 40 min run enough identification detecting first gene.We implemented an effective method (sensitivity 100% specificity 92.3%) removal enrichment (both gram-negative positive) directly milk. To best our knowledge, is culture- amplification-independent nanopore-based metagenomic real-time detection (within hours) profile 5-9 hours), samples. These provide promising potential future on-farm adaptable approach better clinical management
Language: Английский
Citations
14
Molecules, Journal Year: 2023, Volume and Issue: 28(8), P. 3425 - 3425
Published: April 13, 2023
Bovine mastitis is a major concern for the dairy cattle community worldwide. Mastitis, subclinical or clinical, can be caused by contagious environmental pathogens. Costs related to include direct and indirect losses, leading global annual losses of USD 35 billion. The primary treatment represented antibiotics, even if that results in presence residues milk. overuse misuse antibiotics livestock contributing development antimicrobial resistance (AMR), resulting limited resolution treatments, as well serious threat public health. Novel alternatives, like use plant essential oils (EOs), are needed replace antibiotic therapy when facing multidrug-resistant bacteria. This review aims provide an updated overview vitro vivo studies available on EOs their main components antibacterial against variety causing There many studies, but only several vivo. Given promising treatments with EOs, further clinical trials needed.
Language: Английский
Citations
14
Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(6)
Published: Nov. 3, 2023
Emerging evidence suggests that microbiome-targeted approaches may provide a novel opportunity to reduce the incidence of reproductive failures in cattle. To develop such microbiome-based strategies, one first logical steps is identify microbiome features related fertility and isolate fertility-associated microbial species for developing future bacterial consortium could be administered before breeding enhance pregnancy outcomes. Here, we characterized vaginal uterine microbiota beef cattle became pregnant or remained open via artificial insemination identified associated with fertility. We compared similarities between heifers cows. Using culturing, provided new insights into culturable fraction their antimicrobial resistance. Overall, our findings will serve as an important basis research aimed at harnessing improved
Language: Английский
Citations
14
Journal of Global Antimicrobial Resistance, Journal Year: 2024, Volume and Issue: 36, P. 326 - 335
Published: Feb. 1, 2024
The objective of the present study was to examine diversity Staphylococcus aureus from mastitis milk samples cows in Rwanda.
Language: Английский
Citations
5
Food Control, Journal Year: 2024, Volume and Issue: 166, P. 110630 - 110630
Published: June 25, 2024
Language: Английский
Citations
5
International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(19), P. 14587 - 14587
Published: Sept. 26, 2023
Staphylococci are major causes of infections in mammals. Mammals colonized by diverse staphylococcal species, often with moderate to strong host specificity, and colonization is a common source infection. Staphylococcal animals not only importance for animal well-being but have considerable economic consequences, such as the case mastitis, which costs billions dollars annually. Furthermore, pet can be temporary carriers strains infectious humans. Moreover, antimicrobial resistance great concern livestock infections, there antibiotic overuse, resistant transferred With number working antibiotics continuously becoming smaller due concomitant spread strains, alternative approaches, anti-virulence, increasingly being investigated treat infections. For this, understanding virulence mechanisms pathogens crucial. While many factors similar functions humans animals, frequent reports host-specific mechanisms. we beginning understand animal-specific pathogens. This review gives an overview caused staphylococci our knowledge about involved.
Language: Английский
Citations
11
Dairy, Journal Year: 2024, Volume and Issue: 5(1), P. 54 - 65
Published: Jan. 5, 2024
Staphylococcus aureus is a major cause of subclinical mastitis in dairy cows, and its development antibiotic resistance has limited treatment efficacy. Essential oils (EOs) are natural products with wide range antimicrobial properties that could be used to treat bovine mastitis. This study aims investigate the activity EOs against S. isolated from cases State São Paulo—Brazil. A total 14 isolates were selected, based on presence biofilm-forming genes (icaA, icaD, bap), cultured final concentration 103 CFU.mL−1 for minimum inhibitory (MIC) bactericidal (MBC) analysis five (Citrus aurantium bergamia—bergamot, Copaifera reticulata—copaiba, Foeniculum vulgare—fennel, Zingiber officinale—ginger, Ocimum basilicum—basil). The chemical compositions characterized using gas chromatography coupled mass-selective detector (GC/MSD). Basil bergamot exhibited highest strains, mean MIC/MBC values 1.561 ± 0.223/2.806 0.255 mg.mL−1 2.782 0.228/4.396 0.198 mg.mL−1, respectively. primary compounds basil EO methyl-chavicol, linalool, α-humulene, while predominantly contained linalyl acetate, limonene, linalool. research highlights potential as agents treating caused by aureus, offering alternative traditional antibiotics contributing animal welfare public health. In addition, it emphasizes need further studies validate long-term effects, optimal dosages, application methods.
Language: Английский
Citations
4
Veterinary Medicine and Science, Journal Year: 2024, Volume and Issue: 10(2)
Published: Feb. 15, 2024
Abstract Background Mastitis poses significant challenges to the dairy industry, resulting in economic losses and increased veterinary expenses. Staphylococcus aureus is a common cause of bovine mastitis, relying on efficient adhesion biofilm formation for infection. Objectives This study aimed employ meta‐analysis investigate occurrence genes S. associated with as documented previous studies. Methods was done according Preferred Reporting Items Systematic Reviews Meta‐Analyses, examined 22 eligible articles revealed varying prevalence rates isolates from mastitis. Results Among genes, clfB showed highest ( p ‐estimate = 0.905), followed by fnbA 0.689) fnbB 0.502). The icaA icaD also relatively high 0.694 0.814, respectively). Conversely, biofilm‐associated proteins gene had lowest 0.043). Subgroup analyses based mastitis types publication years no differences prevalence. Insufficient data hindered analysis fib , sasG eno bbp genes. Conclusion provides valuable insights managing ‐induced Additionally, larger‐scale research, particularly less‐studied necessary comprehend molecular roles
Language: Английский
Citations
4