Efficient generation of human induced pluripotent stem cells from urine samples of patients with Fragile X syndrome DOI Creative Commons

Olivier Dionne,

Salomé Sabatié,

Fléchère Fortin

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Nov. 22, 2024

Human induced pluripotent stem cells (iPSCs) are a valuable tool for studying human development and diseases. iPSCs can be generated by reprogramming from any somatic cells, however establishing primary cell cultures involve invasive procedures (e.g., skin biopsy) labor-intensive. In this paper, we describe an efficient, reliable, non-invasive method cultivating urine-derived (UDCs) efficiently reprogram them into using feeder-free non-integrative system. This approach has several advantages: (i) UDCs collection culture non-invasive, straightforward, do not require medical personnel; (ii) commercially available Sendai viruses is highly efficient reliable; (iii) demonstrate strong differentiation potential. To showcase the effectiveness of method, iPSC lines three control individuals patients with Fragile X syndrome.

Language: Английский

Advancements and Challenges in Stem Cell Transplantation for Regenerative Medicine DOI Creative Commons

Lingxi Wei,

Wenqi Yan,

Wahid Shah

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(16), P. e35836 - e35836

Published: Aug. 1, 2024

Stem cell transplantation has emerged as a promising avenue in regenerative medicine, potentially facilitating tissue repair degenerative diseases and injuries. This review comprehensively examines recent developments challenges stem transplantation. It explores the identification isolation of various types, including embryonic, induced pluripotent, adult cells derived from multiple sources. Additionally, highlights tissue-specific applications these cells, focusing on bone cartilage regeneration, treatment neurological disorders, management hematological conditions. Future advancements effective resolution current will be crucial fully realizing potential medicine. With responsible ethical practices, field can transform disease injury treatment, ultimately improving quality life for countless individuals.

Language: Английский

Citations

8
Sustainable keratoplasty models using agri-food waste: a hypothesis for transforming biowaste into biomaterials for tissue engineering research DOI Creative Commons
Peter R. Corridon,

Ayman Mobin,

Zayd Hashem

et al.

Frontiers in Sustainable Food Systems, Journal Year: 2025, Volume and Issue: 9

Published: March 26, 2025

With a growing global population, ways to counterbalance the demand for meat products with effective food security and waste management innovative scalable solutions. Concurrently, alarming incidence of end-stage organ failure, limited availability transplantable organs, directives reduce reliance on animal testing underscore need clinically viable sustainable alternatives. Our approach introduces hypothesis-driven, renewable tissue engineering strategy that creates low-cost keratoplasty models derived entirely from agri-food waste. Specifically, we hypothesize abundant by-products, such as eyes bladders, provide practically unlimited readily available supplies corneal tissues urine-derived stem cells (USCs) can be repurposed into cost-effective, relevant Traditional approaches often rely cadaveric tissues, invasive cell sourcing, or expensive commercial lines, which require complex resource-intensive processes, including high-end bioreactor systems manufacturing environments. These requirements limit widespread adoption technological progress needed increase supply keratografts. proposed leverages combination post-mortem bladder harvesting, in turn facilitates decellularization, non-invasive USC differentiation, compartment-specific recellularization help overcome barriers associated traditional seeding generate this type Overall, our perspective suggests way devise transformative resource-efficient engineering, specifically geared toward improving outcomes while offering broader applications regeneration other bodily tissues/organs biotechnological innovation.

Language: Английский

Citations

1
Challenges of therapeutic applications and regenerative capacities of urine based stem cells in oral, and maxillofacial reconstruction DOI Open Access
Gamal Abdel Nasser Atia,

Fatema Rashed,

Ehab S Taher

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 177, P. 117005 - 117005

Published: June 29, 2024

Urine-derived stem cells (USCs) have gained the attention of researchers in biomedical field past few years . Regarding several varieties that been used for this purpose, USCs demonstrated mesenchymal cell-like properties, such as differentiation and immunomodulation. Furthermore, they could be differentiated into lineages. This is very interesting regenerative techniques based on cell therapy. review will embark describing their separation, profiling. We specifically describe characteristics, addition to potential. Then, we introduce explore primary uses USCs. These involve thier utilization a platform produce cells, however, shall concentrate therapeutic, orofacial applications, providing an in-depth evaluation purpose. The final portion address limitations challenges implementation dentistry.

Language: Английский

Citations

6
Bioprinting of hepatic tissue using 3D technology: Transitioning beyond laboratory models to real-world applications in medical treatments DOI
S. Logesh Kumar, Rishabha Malviya

Applied Materials Today, Journal Year: 2024, Volume and Issue: 39, P. 102307 - 102307

Published: July 8, 2024

Language: Английский

Citations

6
Human Induced Lung Organoids: A Promising Tool for Cystic Fibrosis Drug Screening DOI Open Access
Anna Demchenko, Maxim Balyasin,

Aleksandra Nazarova

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 437 - 437

Published: Jan. 7, 2025

Cystic fibrosis (CF) is an autosomal recessive disorder caused by mutations in the CFTR gene. Currently, modulators are most effective treatment for CF; however, they may not be suitable all patients. A representative and convenient vitro model needed to screen therapeutic agents under development. This study, on common mutation, F508del, investigates efficacy of human induced pluripotent stem cell-derived lung organoids (hiLOs) from NKX2.1+ progenitors airway basal cells (hiBCs) as a 3D modulator response assessment forskolin-induced swelling assay. Weak was observed hiLOs hiBCs VX-770/VX-809 VX-770/VX-661, whereas VX-770/VX-661/VX-445 combination resulted highest response, indicating superior function restoration. The ROC analysis FIS assay results revealed optimal cutoff 1.21, with 65.9% sensitivity 71.8% specificity, predictive accuracy 76.4%. In addition, this study compared clinical patients therapy showed similar drug dynamics. Thus, can effectively CF pathology predict patients' specific modulators.

Language: Английский

Citations

0
Functional regeneration strategies of hair follicles: advances and challenges DOI Creative Commons
Xi Chu, Zhentao Zhou,

Xifei Qian

et al.

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 21, 2025

Hair follicles are essential appendages of human skin that function in protection, sensation, thermoregulation and social interactions. The multicellular components, particularly the dermal papilla, matrix bulge housing stem cells, enable cyclic hair growth postnatally. However, miniaturization loss can occur context ageing, trauma various alopecia-related diseases. Conventional treatments involve redistribution existing follicles, which may not be viable patients lacking follicular resources. Recent progress comprehension morphogenesis development biomaterials has significantly advanced follicle reconstruction, incorporating organ germ assembling, cell induction bioprinting techniques. Despite these advancements, fully restoring remains challenging due to complexities replicating embryonic signals sustaining cycles. Identifying suitable sources for clinical applications also presents a hurdle. Here, we retrospect made field regeneration, aiming offer an exhaustive analysis on benefits limitations methods, foster innovative solutions.

Language: Английский

Citations

0
Regenerative Medicine in Plastic Surgery: The Role of Stem Cells and Bioprinting DOI Creative Commons
Kirolos Eskandar

Regenesis repair rehabilitation., Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Mesenchymal stem cells improve cardiac function in diabetic rats by reducing cardiac injury biomarkers and downregulating JAK/STAT/iNOS and iNOS/Apoptosis signaling pathways DOI

Thoraya Mohamed Elhassan A-Elgadir,

Ayed A. Shati,

Saif Aboud Alqahtani

et al.

Molecular and Cellular Endocrinology, Journal Year: 2024, Volume and Issue: 591, P. 112280 - 112280

Published: May 24, 2024

Language: Английский

Citations

3
Editorial: Mesenchymal and induced-pluripotent stem cells as models to study biological processes DOI Creative Commons
Melvin A. Ambele, Catarina Oliveira Miranda

Frontiers in Genetics, Journal Year: 2024, Volume and Issue: 15

Published: July 25, 2024

Keywords: mesenchymal stromal/stem cells, induced-pluripotent stem organoids, disease models, biological processes

Language: Английский

Citations

0
Efficient generation of human induced pluripotent stem cells from urine samples of patients with Fragile X syndrome DOI Creative Commons

Olivier Dionne,

Salomé Sabatié,

Fléchère Fortin

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Nov. 22, 2024

Human induced pluripotent stem cells (iPSCs) are a valuable tool for studying human development and diseases. iPSCs can be generated by reprogramming from any somatic cells, however establishing primary cell cultures involve invasive procedures (e.g., skin biopsy) labor-intensive. In this paper, we describe an efficient, reliable, non-invasive method cultivating urine-derived (UDCs) efficiently reprogram them into using feeder-free non-integrative system. This approach has several advantages: (i) UDCs collection culture non-invasive, straightforward, do not require medical personnel; (ii) commercially available Sendai viruses is highly efficient reliable; (iii) demonstrate strong differentiation potential. To showcase the effectiveness of method, iPSC lines three control individuals patients with Fragile X syndrome.

Language: Английский

Citations

0