Being
the
major
cellular
component
of
highly
dynamic
tissue
endometrial
stromal
cells
(EnSC)
are
exposed
to
cycles
proliferation
upon
hormonal
stimulation
what
might
pose
risks
for
mutations
accumulation
and
malignization.
However,
tumors
rare
unusual
types
tumor.
The
present
study
aimed
uncover
defense
mechanisms
that
underlie
resistance
EnSC
against
oncogenic
transformation.
All
experiments
were
performed
in
vitro
using
following
methods:
FACS,
WB,
RT-PCR,
IF,
molecular
cloning,
lentiviral
transduction,
CRISPR/Cas9
genome
editing.
We
revealed
expression
mutant
HRASG12V
results
senescence
EnSC.
experimentally
confirmed
inability
HRASG12V-expressing
bypass
resume
even
oestrogen
stimulation.
At
level,
induction
oncogene-induced
(OIS)
was
accompanied
by
activation
MEK/ERK,
PI3K/AKT,
p53/p21WAF/CIP/Rb
p38/p16INK4a/Rb
pathways,
however
inhibiting
either
pathway
did
not
prevent
cell
cycle
arrest.
PTEN
loss
established
as
additional
feature
HRASG12V-induced
By
CRISPR-Cas9
mediated
knockout,
we
distinguished
loss-induced
a
reserve
mechanism
transformation
highlights
an
antitumor
controlled
multiple
backup
pathways.
Antioxidants,
Journal Year:
2023,
Volume and Issue:
12(4), P. 901 - 901
Published: April 9, 2023
Colorectal
cancer
(CRC)
represents
the
second
leading
cause
of
cancer-related
deaths
worldwide.
The
pathogenesis
CRC
is
a
complex
multistep
process.
Among
other
factors,
inflammation
and
oxidative
stress
(OS)
have
been
reported
to
be
involved
in
initiation
development
CRC.
Although
OS
plays
vital
part
life
all
organisms,
its
long-term
effects
on
human
body
may
different
chronic
diseases,
including
diseases.
Chronic
can
lead
oxidation
biomolecules
(nucleic
acids,
lipids
proteins)
or
activation
inflammatory
signaling
pathways,
resulting
several
transcription
factors
dysregulation
gene
protein
expression
followed
by
tumor
cell
survival.
In
addition,
it
well
known
that
intestinal
diseases
such
as
bowel
disease
(IBD)
are
associated
with
an
increased
risk
cancer,
link
between
IBD
progression
has
reported.
This
review
focuses
role
causative
agent
colorectal
cancer.
Neuron,
Journal Year:
2024,
Volume and Issue:
112(8), P. 1208 - 1221
Published: Feb. 22, 2024
Alzheimer's
disease
(AD)
and
the
mechanisms
underlying
its
etiology
progression
are
complex
multifactorial.
The
higher
AD
risk
in
women
may
serve
as
a
clue
to
better
understand
these
complicated
processes.
In
this
review,
we
examine
aspects
of
that
demonstrate
sex-dependent
effects
delve
into
potential
biological
responsible,
compiling
findings
from
advanced
technologies
such
single-cell
RNA
sequencing,
metabolomics,
multi-omics
analyses.
We
review
evidence
sex
hormones
chromosomes
interact
with
various
during
aging,
encompassing
inflammation,
metabolism,
autophagy,
leading
unique
characteristics
between
men
women.
Cell Communication and Signaling,
Journal Year:
2023,
Volume and Issue:
21(1)
Published: Aug. 23, 2023
Telomerase
reverse
transcriptase
(TERT/hTERT)
serves
as
the
pivotal
catalytic
subunit
of
telomerase,
a
crucial
enzyme
responsible
for
telomere
maintenance
and
human
genome
stability.
The
high
activation
hTERT,
observed
in
over
90%
tumors,
plays
significant
role
tumor
initiation
progression.
An
in-depth
exploration
hTERT
mechanisms
cancer
holds
promise
advancing
our
understanding
disease
developing
more
effective
treatment
strategies.
In
breast
cancer,
expression
is
regulated
by
epigenetic,
transcriptional,
post-translational
modification
DNA
variation.
Besides
its
canonical
function
maintenance,
exerts
non-canonical
roles
that
contribute
to
progression
through
telomerase-independent
mechanisms.
This
comprehensive
review
summarizes
regulatory
governing
elucidates
functional
implications
activation.
Given
overexpression
most
cells,
detection
associated
molecules
are
potential
enhancing
early
screening
prognostic
evaluation
cancer.
Although
still
stages,
therapeutic
approaches
targeting
show
viable
strategies
treatment.
These
methods
also
discussed
this
paper.
Video
Abstract.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(14), P. 7694 - 7694
Published: July 13, 2024
Telomeres
are
part
of
chromatin
structures
containing
repeated
DNA
sequences,
which
function
as
protective
caps
at
the
ends
chromosomes
and
prevent
degradation
recombination,
thus
ensuring
integrity
genome.
While
telomere
length
(TL)
can
be
genetically
inherited,
TL
shortening
has
been
associated
with
ageing
multiple
xenobiotics
bioactive
substances.
characterised
a
reliable
biomarker
for
predisposition
to
developing
chronic
pathologies
their
progression.
This
narrative
review
aims
provide
arguments
in
favour
including
measurements
complex
prognostic
diagnostic
panel
importance
assessing
effect
different
pharmacologically
active
molecules
on
biology
telomeres.
Medicines
used
management
cardiovascular
diseases,
diabetes,
schizophrenia,
hormone
replacement
therapy
menopause,
danazol,
melatonin,
probiotics
have
studied
positive
effects
against
shortening.
All
these
classes
drugs
analysed
present
review,
particular
focus
molecular
mechanisms
involved.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(4), P. 2152 - 2152
Published: Feb. 10, 2024
Antineoplastic
therapies
for
prostate
cancer
(PCa)
have
traditionally
centered
around
the
androgen
receptor
(AR)
pathway,
which
has
demonstrated
a
significant
role
in
oncogenesis.
Nevertheless,
it
is
becoming
progressively
apparent
that
therapeutic
strategies
must
diversify
their
focus
due
to
emergence
of
resistance
mechanisms
tumor
employs
when
subjected
monomolecular
treatments.
This
review
illustrates
how
dysregulation
lipid
metabolic
pathway
constitutes
survival
strategy
adopted
by
tumors
evade
eradication
efforts.
Integrating
this
aspect
into
oncological
management
could
prove
valuable
combating
PCa.
Expert Review of Molecular Diagnostics,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 1, 2025
The
hTERT
gene
is
an
essential
part
of
the
telomerase
enzyme,
preserving
telomere
length
and
encouraging
cellular
immortality.
study
aimed
to
investigate
whether
TERT
SNP
was
associated
with
increased
risk
lung
cancer
in
North
Indian
population.
387
patients
undergoing
platinum-based
chemotherapy
384
healthy
controls
were
genotyped
for
variant
rs2735940
(T>C)
using
PCR-RFLP.
determine
significant
association
between
genetic
risk.
Patients
carrying
homozygous
mutant
genotype
(CC)
showed
a
(0
R
=
2.4,
p
0.03).
Furthermore,
dominant
model,
combination
(TC+CC)
susceptibility
AOR
1.67
(p
0.0016).
For
rs2735940,
individuals
SCLC
significantly
more
likely
develop
0.0004).
Our
results
also
that
who
received
docetaxel
cisplatin/carboplatin
had
better
prognosis
as
compared
alternative
regimens.
associates'
toxicities
polymorphism
delivering
insights
improving
biomarker
development
individualized
treatment.
Advances in Natural Sciences Nanoscience and Nanotechnology,
Journal Year:
2024,
Volume and Issue:
15(3), P. 035017 - 035017
Published: Aug. 30, 2024
Abstract
Capsaicin
is
a
bioactive
phytochemical
of
red
and
chili
peppers.
It
has
shown
therapeutic
properties,
including
anticancer
activities.
In
this
study,
the
potential
anti-telomerase
effect
capsaicin,
as
well
synergic
inhibitory
compound
in
combination
with
cobalt
ferrite-graphene
oxide
nanocomposites
was
investigated
on
breast
cancer
cell
line.
For
purpose,
ferrite/graphene
(CoFe
2
O
4
/GO)
nanoparticles
were
synthesized
characterized.
Then,
different
concentrations
capsaicin
CoFe
/GO
nanoparticles,
their
adenocarcinoma
lines
(MCF-7
MCF-10A)
analyzed
using
MTT
assay
quantitative
real-time
PCR
for
assessing
viability
expression
changes
telomerase
reverse
transcriptase
(
tert
),
Bax
Bcl2
genes,
respectively.
The
results
showed
synergistic
NPs
MCF-7
that
reduced
IC50
value
from
0.1
1
mg/ml
to
0.05
0.5
mg
ml
−1
,
Moreover,
bcl
genes
decreased
after
treatment;
while
contrast,
bax
gene
significantly
increased.
Consequently,
treatment
could
induce
apoptosis
inhibit
growth
cells.
conclusion,
combinational
be
considered
an
efficient
regimen
cancer.
Journal of Experimental & Clinical Cancer Research,
Journal Year:
2024,
Volume and Issue:
43(1)
Published: Dec. 16, 2024
Abstract
Background
The
androgen
receptor
(AR),
a
ligand-dependent
transcription
factor,
plays
key
role
in
regulating
prostate
cancer
(PCa)
growth.
novel
bipolar
therapy
(BAT)
uses
supraphysiological
levels
(SAL)
that
suppresses
growth
of
PCa
cells
and
induces
cellular
senescence
functioning
as
tumor
suppressive
mechanism.
long
non-coding
RNAs
(lncRNAs)
the
regulation
SAL-mediated
remains
unclear.
This
study
focuses
on
SAL-repressed
lncRNA
MIR503HG
,
examining
its
involvement
androgen-controlled
PCa.
Methods
Transcriptome
ChIP-Seq
analyses
treated
with
SAL
were
conducted
to
identify
SAL-downregulated
lncRNAs.
Expression
analyzed
691
patient
samples,
mouse
xenograft
tumors
patient-derived
xenografts.
Knockdown
overexpression
experiments
performed
assess
proliferation
using
senescence-associated
β-Gal
assays,
qRT-PCRs,
Western
blotting.
activity
was
confirmed
spheroids.
Results
A
large
cohort
analysis
shows
is
overexpressed
metastatic
associated
reduced
survival,
indicating
potential
oncogenic
role.
Notably,
treatment
expression
across
four
different
cell
lines
xenografts
but
interestingly
not
senescence-resistant
LNCaP
Abl
EnzaR
cells.
Functional
assays
reveal
promotes
inhibits
senescence,
partly
through
miR-424-5p.
Mechanistic
rescue
indicate
regulates
AKT-p70S6K
p15
INK4b
-pRb
pathway.
Reduced
by
or
knockdown
resulted
decreased
BRCA2
suggesting
DNA
repair
mechanisms
implications
for
PARP
inhibitor
sensitivity
used
BAT
clinical
trial.
Conclusions
acts
an
regulator
repressing
senescence.
SAL-induced
suppression
enhances
tumor-suppressive
effects
AR
signaling,
could
serve
biomarker
responsiveness
target
combination
therapies
inhibitors.
