Published: Jan. 1, 2024
Language: Английский
Published: Jan. 1, 2025
There is a growing global interest in developing greener and safer pharmaceutical formulations that minimize environmental impact. In this study, we present biotechnological nanoformulation combining prodigiosin (PG), water-insoluble, microbially derived red pigment with multifunctional bioactivity surfactin (SF), lipopeptide biosurfactant known for its diverse surface-active properties. PG was produced by Serratia marcescens, spectrally characterized, subsequently incorporated into preformed SF nanomicelles (nSF) via thermal sonication at 50 °C, yielding PG-loaded (nPG/SF). Physicochemical characterization of nPG/SF revealed spherical nanostructures an average diameter 227 ± 18.95 nm, moderate polydispersity index (PDI) 0.59 0.17, negative surface charge (-27.1 0.97 mV), high entrapment efficiency (98%), physical stability 4 °C least 14 days. terms bioactivity, nanomicellization appeared to enhance the antibacterial effect against Staphylococcus aureus clinical isolate Pseudomonas aeruginosa, while significantly (p < 0.05) improving SF's cytotoxic activity two breast cancer cell lines, MCF-7 MDA-MB-231. Furthermore, using nSF as carrier slightly increased PG’s anticancer activity, suggesting potential dose reduction both SF. Overall, study provides proof concept eco-friendly, biosurfactant-based nanomicellar formulation improved solubility, stability, supporting applications. Further investigations are warranted validate extend these findings.
Language: Английский
Citations
0
International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 144036 - 144036
Published: May 1, 2025
Language: Английский
Citations
0
Journal of Agriculture and Food Research, Journal Year: 2025, Volume and Issue: unknown, P. 101955 - 101955
Published: May 1, 2025
Language: Английский
Citations
0
Colorants, Journal Year: 2025, Volume and Issue: 4(2), P. 18 - 18
Published: May 11, 2025
Globally, insect pests adversely affect approximately 75% of the most important crops. However, widespread use chemical insecticides has significant drawbacks, including non-specific biological activity, toxicity to humans, detrimental effects on beneficial insects, and rapid development resistance. In this context, prodigiosin—a tripyrrolic secondary metabolite produced by various microorganisms—emerges as a promising alternative due its favourable properties, such being non-toxic, environmentally safe, non-irritant, non-allergenic, having non-carcinogenic potential. Prodigiosin demonstrated insecticidal efficiency against at developmental stages. Studies suggest that prodigiosin inhibits enzymes like acetylcholine esterase, protease, acid phosphatase induces oxidative stress. This review explores potential an eco-friendly insecticide, discussing production, extraction, purification processes advantages, disadvantages, mechanism action, future perspectives. Special emphasis is given using non-pathogenic strains mitigate biosafety concerns.
Language: Английский
Citations
0
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15
Published: July 10, 2024
Breast cancer (BC) is the most common malignancy among women and considered a major global health challenge worldwide due to its high incidence mortality rates. Treatment strategies for BC wide-ranging include surgery, radiotherapy, chemotherapy, targeted hormonal therapy immunotherapy. Immunotherapy has gained popularity recently often integrated as component of personalized care because it aims strengthen immune system enable recognize eradicate transformed cells. It fewer side-effects lower toxicity than other treatment strategies, such chemotherapy. Many natural products are being investigated wide range therapeutic pharmacological properties, modulation activity against infection, auto-immune disease, cancer. This review presents an overview response-related pathways in BC, followed by detailed explanation how compounds can act immunomodulatory agents biomolecular targets. Research been carried out on many forms products, including extracts, isolated entities, synthetic derivatives, nanoparticles, combinations compounds. Findings have shown significant regulatory effects cells cytokines that lead immunogenic cell death, well upregulation macrophages CD+8 T cells, increased killer dendritic activity. Natural also found inhibit some immuno-suppressive Treg myeloid-derived suppressor decrease immunosuppressive factors TGF-β IL-10. Also, target hinder checkpoints PD-L1.
Language: Английский
Citations
2
Processes, Journal Year: 2024, Volume and Issue: 12(9), P. 1794 - 1794
Published: Aug. 23, 2024
Prodigiosin is a red bacterial pigment with great potential as natural dye and drug precursor, while presenting several pharmacological properties, including antimicrobial anticancer activities. Its commercialization for biomedical applications, however, remains scarce. The major limitations are related to the lack of efficient bioprocesses scaling up from laboratory production. In present work, upstream process prodigiosin production was developed using marine Serratia rubidaea isolated sample collected near shallow-water hydrothermal vent. yield product per biomass found be influenced by cell concentration in inoculum. system scaled 2 L stirred tank reactors two different vessel geometries. It shown that geometry cascade control mode regulating dissolved oxygen volumetric mass transfer coefficient (kLa) thus To improve yields, strategies mimic aeration conditions at sampling site were tested. When inoculum grown 5 h 200 rpm 19 25 rpm, which significantly decreased available, cells produced 588.2 mgproduct/gbiomass, corresponding 1066.2 mg 24 productivity 36.1 mgproduct/(L.h). This 3.7-fold increase 4.5-fold relation when no particular strategy promoted. Additionally, it lipid analysis flow cytometry may used reliable at-line analytical tools, allowing monitoring condition during fermentation.
Language: Английский
Citations
2
Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15
Published: March 21, 2024
Bioprospecting actinobacterial secondary metabolism from untapped marine sources may lead to the discovery of biotechnologically-relevant compounds. While studying diversity and bioactive potential Actinomycetota associated with Codium tomentosum, a green seaweed collected in northern Portuguese cost, strain CT-F61, identified as Streptomyces violaceoruber , was isolated. Its extracts displayed strong anticancer activity on breast carcinoma T-47D colorectal HCT116 cells, being effective well against panel human fish pathogenic bacteria. Following bioactivity-guided isolation pipeline, new analogue red-pigmented family antibiotics prodigiosins, decylprodigiosin (1), chemically characterized. Despite this natural products well-known for long time, we report first evidence prodigiosins produced by seaweed-associated actinomycete.
Language: Английский
Citations
2
World Journal of Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 40(9)
Published: July 20, 2024
Language: Английский
Citations
2
Heliyon, Journal Year: 2023, Volume and Issue: 9(6), P. e16963 - e16963
Published: June 1, 2023
This research investigates the potentials of prodigiosin(PG) derived from bacteria and its formulations against triple-negative breast (TNB), lung, colon cancer cells. The PG was extracted S. marcescens using continuous batch culture, characterized, formulated into lyophilized parenteral nanoparticles (PNPs). were characterized with respect to entrapment efficiency (EE), DSC, FT-IR, TEM, proton nuclear magnetic resonance (1H NMR) spectroscopy. In vitro drug release evaluated in phosphate buffer (pH 7.4) while acute toxicity, hematological histopathological studies performed on rats. cytotoxicity TNB (MCF-7), lung (A-549), (HT-29) cell lines. High EE (92.3 ± 12%) up 89.4% within 8 h obtained. DSC thermograms PG-PNPs showed endothermic peaks indicating amorphous nature. FT-IR spectrum revealed remarkable pure PG, no strong chemical interaction between excipients. TEM micrograph nano-sized (20–30 nm) whose particles mostly lamellar hexagonal structures. 1H NMR result structure showing all assigned shifts. Toxicity results formulation a concentration 5000 mg/kg insignificant vacuolar changes hepatocytes liver, normal renal medulla cortex kidney. inhibited growth breast, lipid (PG-PNPs) potential delivery treatments.
Language: Английский
Citations
4
Expert Opinion on Drug Delivery, Journal Year: 2024, Volume and Issue: 21(4), P. 663 - 677
Published: April 2, 2024
Background Chemo-immunotherapy modifies the tumor microenvironment to enhance immune response and improve chemotherapy. This study introduces a dual-armed chemo-immunotherapy strategy combating breast progression while re-polarizing Tumor Associated Macrophage (TAM) using Prodigiosin loaded mannan-coated magnetic nanoparticles (PG@M-MNPs).
Language: Английский
Citations
1