Evaluation of the Effect of MiR-206 on the Expression of Toll-like Receptor 2 and TLR4 in the Melanoma A-375 Cells DOI

Mojtaba Gholinezhad,

Bahareh Sartipi,

Babak Sandoghchian Shotorbani

et al.

Biomedical Research Bulletin, Journal Year: 2023, Volume and Issue: 1(4), P. 130 - 134

Published: Dec. 29, 2023

Background: Melanoma is one of the most deadly skin cancers in humanity, which imposes extensive costs on governments. Fortunately, death rate has decreased despite increasing incidence melanoma. Various treatment methods, including surgery, chemotherapy, radiotherapy, targeted therapy, and like, are used to treat this disease. However, recent advances diagnosis treatment, melanoma remains leading causes world. One new methods based microRNAs, miRNA 206. The aim study was determine effect MIR-206 Toll-like receptor 2 (TLR2) TLR4 cell line (A-375). Methods: present vitro performed cancer cells RPMI640 culture medium containing 10% fetal calf serum at 37 ° C 5% CO2 . After counting dividing cells, inside plate were stimulated over time with protein. Incubation performed. 48 hours, collected, total mRNA extracted, expression levels TLR2 examined by real-time polymerase chain reaction. Results: results demonstrated that IL6 (A-375) exposure MIR-206. also showed nuclear factor kappa B line. Conclusion: findings confirmed role as a tumor suppressor cancer. These suggest may be for near future. Accordingly, can considered potential target gene therapy patients cancer, advantages chemotherapy reduction cytotoxicity its invasiveness.

Language: Английский

Serum exosomes miR-206 and miR-549a-3p as potential biomarkers of traumatic brain injury DOI Creative Commons
Yajun Yang, Yi Wang, Panpan Li

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 2, 2024

Abstract Traumatic brain injury (TBI) is one of the leading causes death and disability worldwide. However, effective diagnostic, therapeutic prognostic biomarkers are still lacking. Our research group previously revealed through high-throughput sequencing that serum exosomes miR-133a-3p, miR-206, miR-549a-3p differ significantly in severe TBI (sTBI), mild or moderate (mTBI), control groups. convincing experimental evidence To solve this problem, we used qPCR study to further verify expression levels miR-206 patients. The results showed good predictive value as TBI. In addition, order whether can be potential patients with understand mechanism their possible effects, determined contents SOD, BDNF, VEGF, VEGI, NSE S100β that, correlation S100β. conclusion, have serve

Language: Английский

Citations

7
Unraveling the Role of microRNAs in the Metastatic Landscape of Gastric Cancer DOI Creative Commons

Arefeh Zabeti Touchaei,

Sogand Vahidi

Published: Jan. 1, 2025

Gastric cancer (GC) continues to pose a major health issue worldwide, and diagnoses made at advanced stages frequently result in unfavorable outcomes. The metastatic spread of GC presents substantial therapeutic challenges, necessitating deeper understanding the tumor microenvironment (TME) its interactions with cells. MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression have been identified as key factors metastasis GC. This review explores biogenesis functional mechanisms miRNAs, highlighting their dual roles oncogenes suppressors metastasis. We discuss specific miRNAs facilitate progression by influencing pathways related invasion, angiogenesis, immune evasion. Furthermore, we examine potential diagnostic prognostic biomarkers, offering insights into utility timely identification tailored approaches. By elucidating complex regulatory networks involving this underscores significance both targets biomarkers management gastric cancer.

Language: Английский

Citations

0
Role of miRNAs in Cervical Cancer DOI
Sachin Shetty, Ayush Khandelwal,

Roopal Behera

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
The multifaceted roles of extracellular vesicles in osteonecrosis of the femoral head DOI

Hongxu Li,

Haoyang Liu, Yu Zhou

et al.

Journal of Orthopaedic Translation, Journal Year: 2025, Volume and Issue: 52, P. 70 - 84

Published: April 10, 2025

Language: Английский

Citations

0
miRNA-Mediated Mechanisms in the Generation of Effective and Safe Oncolytic Viruses DOI Creative Commons

Мария Сергеевна Торопко,

Sergey Chuvpilo,

Alexander Karabelsky

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(8), P. 986 - 986

Published: July 25, 2024

MicroRNAs (miRNAs) are short non-coding RNAs that regulate gene expression by inhibiting the translation of target transcripts. The profiles miRNAs vary in different tissues and change with development diseases, including cancer. This feature has begun to be used for modification oncolytic viruses (OVs) order increase their selectivity efficacy. OVs represent a relatively new class anticancer drugs; they designed replicate cancer tumors destroy them. These can natural within tumor cells, or recombinant created laboratories. There some concerns regarding OVs’ toxicity, due ability partially healthy tissues. In addition, lytic immunological responses upon OV therapy not always sufficient, so various editing methods used. review discusses latest results preclinical clinical studies OVs, modifications which associated miRNA-mediated mechanism silencing.

Language: Английский

Citations

3
Zfp697 is an RNA-binding protein that regulates skeletal muscle inflammation and remodeling DOI Creative Commons
Jorge C. Correia, Paulo R. Jannig, Maya L. Gosztyla

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(34)

Published: Aug. 14, 2024

Skeletal muscle atrophy is a morbidity and mortality risk factor that happens with disuse, chronic disease, aging. The tissue remodeling during recovery from or injury involves changes in different cell types such as fibers, satellite immune cells. Here, we show the previously uncharacterized gene protein Zfp697 damage-induced regulator of remodeling. Zfp697/ZNF697 expression transiently elevated mice humans. Sustained mouse leads to signature chemokine secretion, recruitment, extracellular matrix Notably, although expressed several skeletal muscle, myofiber-specific genetic ablation sufficient hinder inflammatory regenerative response injury, compromising functional recovery. We an essential mediator interferon gamma cells it functions primarily RNA-interacting protein, very high number miRNA targets. This work identifies integrator cell–cell communication necessary for regeneration.

Language: Английский

Citations

3
Evidence for the Contribution of the miR-206/BDNF Pathway in the Pathophysiology of Depression DOI Creative Commons

Yabin Zheng,

Xiang Jin

The International Journal of Neuropsychopharmacology, Journal Year: 2024, Volume and Issue: 27(10)

Published: Sept. 2, 2024

Depression is a complex disorder with substantial impacts on individual health and has major public implications. results from interactions between genetic environmental factors. Epigenetic mechanisms, including DNA methylation, microRNAs (miRNAs), histone modifications, can produce heritable phenotypic changes without change in sequence recently were proven to mediate lasting increases the risk of depression following exposure adverse life events. Of these, miRNAs are gaining attention for their role pathogenesis many stress-associated mental disorders, depression. One such miRNA microRNA-206 (miR-206), which critical candidate increasing susceptibility stress. Although miR-206 thought be typical muscle-specific miRNA, it expressed throughout brain, particularly hippocampus prefrontal cortex. Until now, only few studies have been conducted rodents understand stress-related abnormalities neurogenesis. However, precise underlying molecular mechanism miR-206-mediated depression-like behaviors remains largely unknown. Here, we reviewed recent advances field biomedical clinical research using different tissues various experimental designs described how expression these affect neuronal functions. Moreover, focused investigating brain-derived neurotrophic factor (BDNF) as functional target miR-206, where implicated by suppressing BDNF. In summary, confirm existence tight correlation miR-206/BDNF pathway.

Language: Английский

Citations

3
circGFRA1: A circular RNA with important roles in human carcinogenesis DOI
Sheyda Khalilian, Zahra Mohajer, Mohammad Amin Khazeei Tabari

et al.

Pathology - Research and Practice, Journal Year: 2023, Volume and Issue: 248, P. 154588 - 154588

Published: June 1, 2023

Language: Английский

Citations

7
Oxidative State in Cutaneous Melanoma Progression: A Question of Balance DOI Creative Commons
Mascia Benedusi, Heaji Lee, Yunsook Lim

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(9), P. 1058 - 1058

Published: Aug. 30, 2024

Reactive oxygen species (ROS) are highly bioactive molecules involved not only in tissue physiology but also the development of different human conditions, including premature aging, cardiovascular pathologies, neurological and neurodegenerative disorders, inflammatory diseases, cancer. Among tumors, cutaneous melanoma, most aggressive lethal form skin cancer, is undoubtedly one well-known "ROS-driven tumor", which main causes represented by ultraviolet (UV) rays' exposure. Although role excessive ROS production melanoma pro-tumorigenic cell fate now well established, little known about its contribution to progression metastatic process. Increasing evidence suggests a dual progression: may enhance cellular growth promote therapeutic resistance, at same time, it can have cytotoxic effects on cancer cells, inducing their apoptosis. In this context, aim present work was focus relationship between redox state signaling pathways directly processes. addition, oxidative or antioxidant strategies for were reviewed discussed.

Language: Английский

Citations

2
Beyond ribosome biogenesis: noncoding nucleolar RNAs in physiology and tumor biology DOI Creative Commons
Nuray Böğürcü,

Nadja Ritschel,

Till Acker

et al.

Nucleus, Journal Year: 2023, Volume and Issue: 14(1)

Published: Oct. 31, 2023

The nucleolus, the largest subcompartment of nucleus, stands out from nucleoplasm due to its exceptionally high local RNA and low DNA concentrations. Within this central hub nuclear metabolism, ribosome biogenesis is most prominent ribonucleoprotein (RNP) process, critically determining structure function nucleolus. However, recent studies have shed light on other roles exploring interplay with various noncoding RNAs that are not directly involved in synthesis. This review focuses intriguing topic summarizes techniques study latest findings nucleolar long (lncRNAs) as well microRNAs (miRNAs) context nucleolus biology beyond biogenesis. We particularly focus multifaceted physiology tumor biology.

Language: Английский

Citations

5