Heliyon, Journal Year: 2024, Volume and Issue: 10(22), P. e40473 - e40473
Published: Nov. 1, 2024
Arsenic can cause extensive toxic damage after entering the body of humans and animals by altering a variety events. As most common form methylation modification RNA in eukaryotic cells, N
Language: Английский
Biology Direct, Journal Year: 2023, Volume and Issue: 18(1)
Published: Sept. 1, 2023
Hepatocellular carcinoma (HCC) is one of the most common and challenging cancers in world. N6-methyladenosine (m6A) modification long non-coding RNAs (lncRNAs) play critical roles progression HCC. However, there are few reports on genome-wide screening functional annotations m6A-methylated lncRNAs HCC.The expression levels m6A methyltransferase METTL3 association with prognosis HCC were determined by RT-qPCR, public dataset platforms. Then, RNA-seq, Pearson correlation analysis, MeRIP-qPCR, RNA half-life assay, gene site-directed mutation, RIP assay RT-qPCR analysis employed to determine downstream target Subsequently, lncRNA glucosylceramidase beta pseudogene 1 (GBAP1) Kaplan-meier curves, vitro experiments vivo tumorigenesis lung metastasis models. pathway GBAP1 explored GO biological process, KEGG enrichment, luciferase reporter rescue so on.METTL3 was upregulated closely related prognosis. And induced acting as writer IGF2BP2 worked its reader. Clinically, significantly associated tumor size, venous infiltration, TNM stage HCC, Functionally, promoted growth both vivo. Furthermore, acted molecular sponge for miR-22-3p increase bone morphogenetic protein receptor type 1A (BMPR1A), which then activated BMP/SMAD cells.Our findings demonstrated that METTL3-induced migration, invasion proliferation cells via GBAP1/miR-22-3p/BMPR1A/SMAD axis. could be a potential indicator therapeutic
Language: Английский
Citations
21
Cell Proliferation, Journal Year: 2023, Volume and Issue: 57(4)
Published: Nov. 14, 2023
Abstract Drug resistance is perhaps the greatest obstacle in improving outcomes for cancer patients, leading to recurrence, progression and metastasis of various cancers. Exploring underlying mechanism worth further study. N6‐methyladenosine (m6A) most common RNA modification found eukaryotes, playing a vital role translation, transportation, stability, degradation, splicing processing. Long noncoding (lncRNA) refers group transcripts that are longer than 200 nucleotides (nt) typically lack ability code proteins. LncRNA has been identified play significant regulating multiple aspects tumour development progression, including proliferation, metastasis, metabolism, treatment. In recent years, growing body evidence emerged, highlighting crucial interplay between m6A lncRNA determining sensitivity cells chemotherapeutic agents. this review, we focus on advancements interaction modulation drug resistance. Additionally, aim explore mechanisms involved process. The objective review provide valuable insights suggest potential future directions reversal chemoresistance cancer.
Language: Английский
Citations
18
Cancer Letters, Journal Year: 2024, Volume and Issue: unknown, P. 217194 - 217194
Published: Aug. 1, 2024
Language: Английский
Citations
5
Research, Journal Year: 2024, Volume and Issue: 7
Published: Jan. 1, 2024
The methylation of adenosine base at the nitrogen-6 position is referred to as “N6-methyladenosine (m 6 A)” and one most prevalent epigenetic modifications in eukaryotic mRNA noncoding RNA (ncRNA). Various m A complex components known “writers,” “erasers,” “readers” are involved function A. Numerous studies have demonstrated that plays a crucial role facilitating communication between different cell types, hence influencing progression diverse physiological pathological phenomena. In recent years, multitude functions molecular pathways linked been identified osteogenic, adipogenic, chondrogenic differentiation bone mesenchymal stem cells (BMSCs). Nevertheless, comprehensive summary these findings has yet be provided. this review, we primarily examined alteration transcripts associated with transcription factors (TFs), well other genes BMSCs. Meanwhile, mutual interactive network modification, miRNAs, lncRNAs was intensively elucidated. last section, given beneficial effect modification osteogenesis chondrogenesis BMSCs, expounded upon potential utility A-related therapeutic interventions identification management human musculoskeletal disorders manifesting cartilage destruction, such osteoporosis, osteomyelitis, osteoarthritis, defect.
Language: Английский
Citations
4
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: March 14, 2024
Abstract As the most prevalent epitranscriptomic modification, N 6 -methyladenosine (m A) shows important roles in a variety of diseases through regulating processing, stability and translation target RNAs. However, potential contributions m A to RNA functions are unclear. Here, we identified functional prognosis-related A-modified SREBF2-AS1 hepatocellular carcinoma (HCC). The expression SREBF2 HCC tissues cells was measured by RT-qPCR. modification level methylated immunoprecipitation assay. progression sorafenib resistance were investigated proliferation, apoptosis, migration, cell viability assays. regulatory mechanisms on Chromatin isolation purification, immunoprecipitation, CUT&RUN, bisulfite DNA sequencing Our findings showed that increased cells, positively correlated with poor survival patients. also prognosis METTL3 METTL14-induced upregulated increasing transcript stability. Functional assays only A-modified, but not non-modified promoted resistance. Mechanistic investigations revealed bound recruited reader FXR1 5-methylcytosine dioxygenase TET1 promoter, leading demethylation at promoter upregulation transcription. rescue critical mediator oncogenic HCC. Together, this study exerted inducing transcriptional activation , suggested as prognostic biomarker therapeutic for
Language: Английский
Citations
4
Bioorganic & Medicinal Chemistry, Journal Year: 2024, Volume and Issue: 113, P. 117922 - 117922
Published: Sept. 13, 2024
Language: Английский
Citations
4
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3078 - 3078
Published: March 27, 2025
Colorectal cancer (CRC) is a leading cause of cancer-related morbidity and mortality, influenced by both genetic epigenetic factors. Long non-coding RNAs (lncRNAs) such as GAS5 CASC8 have been implicated in susceptibility. This study aimed to assess the association rs145204276 ins/del rs10505477 A>G polymorphisms with CRC risk Romanian population. A case-control was conducted, including 156 patients 195 healthy controls. Genotyping for performed using real-time PCR, evaluated logistic regression calculate odds ratios (OR) 95% confidence intervals (CI). The carriers del allele significantly associated increased (OR: 2.13, CI: 1.24-3.63, p = 0.005) dominant model. In subgroup analysis, polymorphism restricted distal colon cases 2.98, 1.57-5.66, 0.001), advanced tumor stages (III + IV) 2.54, 1.31-4.91, 0.007), poorly differentiated tumors (G3) 3.98, 1.49-10.59, 0.009). No significant correlation found polymorphism. may influence susceptibility, particularly stages. However, showed no this cohort.
Language: Английский
Citations
0
Clinical and Experimental Medicine, Journal Year: 2025, Volume and Issue: 25(1)
Published: April 1, 2025
Language: Английский
Citations
0
Modern Rheumatology, Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 16, 2024
The commonest type of eukaryotic RNA modification, N6-methyladenosine (m6A), has drawn increased scrutiny in the context pathological functioning as well relevance determination stability, splicing, transportation, localization, and translation efficiency. m6A modification plays an important role several types arthritis, especially osteoarthritis rheumatoid arthritis. Recent studies have reported that regulates arthritis pathology cells, such chondrocytes synoviocytes via immune responses inflammatory through functional proteins classified writers, erasers, readers. aim this review was to highlight recent advances relevant pathogenesis detail underlying molecular mechanisms, regulatory functions, clinical applications, future perspectives with providing a foundation for research directions.
Language: Английский
Citations
1
Biochimica et Biophysica Acta (BBA) - General Subjects, Journal Year: 2024, Volume and Issue: unknown, P. 130714 - 130714
Published: Sept. 1, 2024
Language: Английский
Citations
1