Phytochemical Analysis, Cytotoxicity, and Antitrypanosomal, Antioxidant, and Anti‐Inflammatory Activities of Clausena anisata Fruit, Azadirachta indica Leaf, and Stem Bark Extracts DOI Creative Commons
Emmanuel Kofi Kumatia, Felix Kwame Zoiku, Prince Kyei Baffour

et al.

Journal of Parasitology Research, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

Human African trypanosomiasis (HAT) is an infectious disease which kills humans and animals as a result of hematological distortions, oxidative stress, tissue neuroinflammations. This study reports on the differences in cytotoxicity, antitrypanosomal, antioxidant, anti‐inflammatory activities ethanol extracts from Clausena anisata fruit (CFE), Azadirachta indica (neem) leaf (NLE), stem bark (NSBE), medicinal plants used to treat HAT its endemic communities. The remarkably inhibited Trypanosoma brucei (GUTat 3.1) parasite vitro with CFE recording highest effect IC 50 0.0055 (0.0955) μ g / mL. standard Coptis japonicum was 0.5957 (0.0693) Also, antitrypanosomal activity NLE 123.34% higher than that NSBE. percentage number wells containing viable T. b. parasites very significantly ( p < 0.001) reduced for all after 48 h incubation. Furthermore, did not show cytotoxicity against liver (HepG2) cells (CC s > 100 g/mL SI = 13.12–32,025.45). NSBE contained quantity phenolic compounds flavonoids also produced antioxidant DPPH free radical scavenging assay (IC 4.99 ± 0.018) protein denaturation 0.1805 0.0002 g/mL). In addition, phytochemical analysis showed classes constituents (seven) among extracts. These results indicate CFE, NLE, possessed significant their actions. However, different mechanism involved since greater demonstrated activities. Due remarkable action are being isolated possible development into novel agents.

Language: Английский

Aqueous extract of Enantia chlorantha Oliv. demonstrates antimalarial activity and improves redox imbalance and biochemical alterations in mice DOI Creative Commons
Ikponmwosa Owen Evbuomwan, Oluyomi Stephen Adeyemı, Olarewaju M. Oluba

et al.

BMC Complementary Medicine and Therapies, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 24, 2025

Malaria is an infectious disease, which has continued to cause inconceivable loss of lives every year, almost unabatedly. Currently, it become more difficult treat the disease due emergence and spread resistance recommended antimalarial drugs. This situation necessitates urgent search for compounds with unique modes action. Here, we investigate activity, antioxidant anti-inflammatory capacity Enantia chlorantha aqueous stem bark extract (EcASBE) in vivo. The was screened selected phytoconstituents including alkaloids flavonoids. We evaluated activity EcASBE against Plasmodium berghei NK65 infection mice, using curative, prophylactic, suppressive test models, respectively. In addition, activities were assessed. significantly (p < 0.05) inhibited parasitaemia dose-dependently, highest inhibition (80.4%) prolonged survival (MST = 20) observed curative test. Our findings reveal significant improvement serum ALT, AST, ALP, GGT, levels TNF-α, creatinine urea following administration. Furthermore, led a rise CAT, SOD, GPx, GSH, concomitant reduction NO MDA levels. antimalarial, antioxidative, antiperoxidative, inflammatory-inhibiting properties plant infected mice demonstrate its great value therapeutic intervention, substantiate use traditional medicine malaria treatment. Hence, further investigation identify repertoire active components warranted.

Language: Английский

Citations

0
Coumarins and related compounds from the medicinal plants of Africa and drug discovery DOI
Simplice Joel N. Tatsimo, A. Fouda,

Souleymanou Neï

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 287 - 323

Published: Jan. 1, 2025

Language: Английский

Citations

0
Insights into Tissue-Specific Specialized Metabolism in Wampee (Clausena lansium (Lour.) Skeels) Varieties DOI Creative Commons
Ran Zhang, Junjie Zhou, Xiaoxuan Zhang

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(19), P. 3092 - 3092

Published: Sept. 27, 2024

Wampee (Clausena lansium (Lour.) Skeels) has natural bioactive components with diverse health benefits, but its detailed metabolism and tissue distribution are not fully understood. Here, widely targeted metabolomics analysis methods were employed to analyze the wampee fruit (peel, pulp, seed) of 17 different varieties. A total 1286 metabolites annotated, including lipids, flavonoids, polyphenols, carbazole alkaloids, coumarins, organic acids, among others. The quantitative matrix-assisted laser desorption/ionization–mass spectrometry imaging (MALDI-MSI) indicated remarkable variations in metabolite categories content peel, seed fruit. Additionally, difference found that metabolic peel contributed dominantly differences varieties, 7 potential biomarkers identified. In this study, a comprehensive metabolome landscape was established, which provided important information for isolation identification functional components, food industry application, nutritional improvement breeding.

Language: Английский

Citations

2
Susceptibility Patterns in Staphylococcus and Klebsiella Causing Nosocomial Infections upon Treatment with E-Anethole-Rich Essential Oil from Clausena anisata DOI Creative Commons

François Nguimatsia,

Evariste Josué Momo,

Paul Keilah Lunga

et al.

Drugs and Drug Candidates, Journal Year: 2024, Volume and Issue: 3(1), P. 244 - 255

Published: March 1, 2024

High rates of resistance to antibiotics are associated with healthcare-related infections, thus demonstrating the urgent need for effective antimicrobials against these maladies. The present study aims determine chemical composition essential oil (EO) from Clausena anisata leaves and evaluate their antibacterial activity selected nosocomial bacteria. To this end, one kilogram (1 kg) fresh C. was washed boiled 500 mL distilled water 2−4 h using a Clevenger apparatus. then collected in an Erlenmeyer, dried anhydrous sodium sulfate, bottled tinted glass bottle refrigerated at 4 °C before analysis. Next, as-prepared analyzed gas chromatography-mass spectrometry (GC-MS). further tested panel bacteria, including Staphylococcus Klebsiella species, among others, by microdilution resazurin assay minimum inhibitory bactericidal concentrations (MICs MBCs, respectively). As result, 0.77% EO extracted anisata. GC-MS analysis revealed that contained E-anethole (70.77%), methyl isoeugenol (13.85%), estragole (4.10%), γ-terpinene (3.33%), myrcene (2.82%) sabinene (0.77%), being major constituent. Twenty-two compounds were identified through chromatography. Upon testing pathogens, E-anethole-rich exhibited MIC MBC values ranging 3.91 125 µg/mL 7.81 µg/mL, respectively, indicative orientation plant’s (MIC/MBC ratio < 4). This novel contribution highlights scientific validation use traditional treatment various infectious diseases. However, toxicity pharmacokinetic studies, mechanistic bases action, vivo experiments should be investigated successfully plant

Language: Английский

Citations

1
Stage -Specific Antimalarial Activity of Alkaloidal Fractions of the Avicennia africana P. Beauv. (Avicenniaceae) Leaf Extract DOI Creative Commons

Mustapha Abubakar Ahmed,

Francis Ackah Armah, George Ghartey‐Kwansah

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 2, 2024

Abstract Background The global call for malaria eradication rested on finding drugs that not only act against asexual but also sexual forms of the parasite. drawbacks in disease control and prevention due to drug-resistant clones parasite piqued our interest exploring alternative antimalarial from mangrove resources. Aims This study evaluates stage-specific anti-malarial cytotoxic activities fractions crude alkaloidal extracts Avicennia africana leaves. Methods (AAA AAQ) A. were fractionated using column chromatography further analysed GC-mass spectroscopy. then tested activity trophozoites, schizonts, gametocyte stages chloroquine-sensitive strains 3D7 P. falciparum SYBR Green 1 assay. effects evaluated MTT-based Results AAA1-AAA5 AAQ1-AAQ5 produced promising trophozoitocidal with an IC50 value range 0.399–45.690 µg/mL, artesunate (ref drug) yielding 0.09x103 µg/mL. schizonticidal gametocytocidal selected demonstrated high potency IC50s 0.622–18.820 µg/mL drugs) 1.800x10-3 5.100x10-3 respectively. effect CC50 was higher than 100 negligible cytotoxicity erythrocytes SI ranged 2.189 280.899. major compounds identified AAA1, AAQ1, AAQ2 8-carbomoylquinoline, razoxane, dexrazoxane, Conclusion exhibited trophozoitocidal, schizonticidal, no significant RBCs. Quinoline-based alkaloids iron chelators this plant are implicated as possible lead-compound transmission blockers

Language: Английский

Citations

1
Aqueous extract of Enantia chlorantha Oliv. demonstrates antimalarial activity and improves redox imbalance and biochemical alterations in mice DOI Creative Commons
Ikponmwosa Owen Evbuomwan, Oluyomi Stephen Adeyemı, Olarewaju M. Oluba

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 22, 2024

Abstract Background: Malaria is an infectious oxidative disease, which has continued to cause inconceivable loss of lives every year, almost unabatedly. Currently, it become more difficult treat the disease due emergence and spread resistance recommended antimalarial drugs including ACTs, necessitating urgent search for compounds with unique modes action. Here, we investigated activity, antioxidant antiinflammatory capacity Enantia chlorantha aqueous stem bark extract (EcASBE) in vivo. Methods: The was screened selected phytoconstituents alkaloids flavonoids. We evaluated activity EcASBE against Plasmodium berghei NK65 infection in mice, using curative, prophylactic, suppressive test models, respectively. In addition, activities were assessed. Results: The significantly (p < 0.05) inhibited parasitaemia dose-dependently, highest inhibition (80.4%) prolonged survival (MST=20) observed curative test. Our findings reveal significant improvement serum ALT, AST, ALP, GGT, levels TNF-α, creatinine urea following administration. Furthermore, led a rise CAT, SOD, GPx, GSH, concomitant reduction NO MDA levels. Conclusion: antimalarial, antioxidative, antiperoxidative, inflammatory-inhibiting properties plant infected mice demonstrate its great value therapeutic intervention, substantiate use traditional medicine malaria treatment. Hence, further investigation identify repertoire active components warranted.

Language: Английский

Citations

1
In vitro and in silico anti-malarial activity and cytotoxicity of n-hexyl 1-O-rutinoside (a glycoside) isolated from Annickia polycarpa (DC.) Setten and Maas ex I.M. Turner (Annonaceae) DOI
Emmanuel Kofi Kumatia, Felix Kwame Zoiku, Alex Asase

et al.

Journal of Ethnopharmacology, Journal Year: 2023, Volume and Issue: 319, P. 117287 - 117287

Published: Oct. 10, 2023

Language: Английский

Citations

2
Phytochemical analysis, cytotoxicity, anti-trypanosomal, anti-oxidant and anti-inflammatory activities of Clausena anisata fruit, Azadirachta indica leaf and stem bark extracts DOI Creative Commons

Emmanuel Kofi Kumatia,

Felix Kwame Zoiku,

Kyei Baffour

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 12, 2024

Abstract Human African Trypanosomiasis (HAT) is an infectious disease which kills humans and animals as a result of hematological distortions, oxidative stress, tissue neuro inflammations. This study reports on the difference in cytotoxicity, anti-trypanosomal, anti-oxidant anti-inflammatory activities ethanol extracts from Clausena anisata (CFE), Azadirachta indica (Neem) leaf (NLE) stem bark (NSBE), medicinal plants used to treat HAT endemic communities. The remarkably inhibited Trypanosomal brucei brucei (Gutat 3.1) parasite vitro with CFE recording highest effect IC50 0.0055 (0.0955) µg/mL standard Coptis japonicum, was 0.5957 (0.0693) µg/mL. Also, anti-trypanosomal activity NLE 123.34% higher than that NSBE. Percentage number wells containing viable T. b. parasites were very significantly (P < 0.001) reduced for all after 48 h incubation. Furthermore, did not show cytotoxicity against liver (HepG2) cells (CC50s > 100 µg/mL SI = 13.12–32,025.45). NSBE contained quantity phenolic compounds flavonoids also produced DPPH free radical scavenging assay (IC50 4.99 ± 0.018) protein denaturation 0.1805 0.0002 µg/mL). In addition, phytochemical analysis showed classes constituents (7) among extracts. These results indicates CFE, possessed significant their actions. However, different mechanism involved NLE, since greater demonstrated activities. Due remarkable action its are being isolated possible development into novel agents.

Language: Английский

Citations

0
Phytochemical Analysis, Cytotoxicity, and Antitrypanosomal, Antioxidant, and Anti‐Inflammatory Activities of Clausena anisata Fruit, Azadirachta indica Leaf, and Stem Bark Extracts DOI Creative Commons
Emmanuel Kofi Kumatia, Felix Kwame Zoiku, Prince Kyei Baffour

et al.

Journal of Parasitology Research, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

Human African trypanosomiasis (HAT) is an infectious disease which kills humans and animals as a result of hematological distortions, oxidative stress, tissue neuroinflammations. This study reports on the differences in cytotoxicity, antitrypanosomal, antioxidant, anti‐inflammatory activities ethanol extracts from Clausena anisata fruit (CFE), Azadirachta indica (neem) leaf (NLE), stem bark (NSBE), medicinal plants used to treat HAT its endemic communities. The remarkably inhibited Trypanosoma brucei (GUTat 3.1) parasite vitro with CFE recording highest effect IC 50 0.0055 (0.0955) μ g / mL. standard Coptis japonicum was 0.5957 (0.0693) Also, antitrypanosomal activity NLE 123.34% higher than that NSBE. percentage number wells containing viable T. b. parasites very significantly ( p < 0.001) reduced for all after 48 h incubation. Furthermore, did not show cytotoxicity against liver (HepG2) cells (CC s > 100 g/mL SI = 13.12–32,025.45). NSBE contained quantity phenolic compounds flavonoids also produced antioxidant DPPH free radical scavenging assay (IC 4.99 ± 0.018) protein denaturation 0.1805 0.0002 g/mL). In addition, phytochemical analysis showed classes constituents (seven) among extracts. These results indicate CFE, NLE, possessed significant their actions. However, different mechanism involved since greater demonstrated activities. Due remarkable action are being isolated possible development into novel agents.

Language: Английский

Citations

0