Biomedicine & Pharmacotherapy,
Journal Year:
2020,
Volume and Issue:
125, P. 109865 - 109865
Published: Feb. 12, 2020
The
pathogenesis
of
ovarian
cancer
remains
to
be
elucidated.
Our
previous
study
demonstrated
that
myosin
heavy
chain
9
(MYH9)
overexpression
was
associated
with
poor
prognosis
epithelial
cancer.
However,
the
mechanism
MYH9
and
its
regulation
by
microRNA
(miR)
is
not
clear.
results
present
miR-6089
one
microRNAs
targeting
MYH9,
suppressed
cell
proliferation,
migration,
invasion
metastasis
in
vivo
vitro.
Mechanistic
studies
confirmed
directly
targeted
inactivate
Wnt/β-catenin
signalling
pathway
downstream
epithelial-to-mesenchymal
transition
(EMT),
cell-cycle
factors
c-Jun,
whereas
reversed
inhibitory
effects
cells
upregulating
EMT,
c-Jun.
Interestingly,
transcriptionally
inhibited
a
transcription
factor
which
could
induced
via
pathway.
Thus
miR-6089/MYH9/β-catenin/c-Jun
formed
negative
feedback
loop
In
clinical
samples,
negatively
correlated
expression.
first
demonstrate
serves
as
tumor-suppressive
miRNA,
inhibits
carcinogenesis
progression.
Journal of Experimental & Clinical Cancer Research,
Journal Year:
2019,
Volume and Issue:
38(1)
Published: March 12, 2019
Cancer
stem
cells
(CSCs)
play
an
important
role
in
the
development
of
pancreatic
cancer.
We
previously
showed
that
microRNA
miR-137
is
downregulated
clinical
samples
cancer,
and
its
expression
negatively
regulates
proliferation
invasiveness
cancer
cells.The
stemness
features
was
detected
by
flow
cytometry,
immunofluorescence
sphere
formation
assay.
Xenograft
mouse
models
were
used
to
assess
vivo.
Dual-luciferase
reporter
assays
determine
how
KLF12.
Bioinformatics
Chromatin
immunoprecipitation
analysis
KLF12
recruitment
DVL2
promoters.
Involvement
Wnt/β-catenin
pathways
investigated
western
blot
Immunohistochemistry.miR-137
inhibits
cell
vitro
as
target
CSC
phenotype
cells.
Suppression
signalling.
correlates
with
canonical
Wnt
pathway
cancer.Our
results
suggest
reduces
Targeting
KLF12-associated
may
identify
new
diagnostic
therapeutic
targets
Aging,
Journal Year:
2017,
Volume and Issue:
9(3), P. 999 - 1011
Published: March 21, 2017
Emerging
studies
have
shown
that
long
noncoding
RNAs
(lncRNAs)
play
critical
roles
in
carcinogenesis
and
progression,
including
human
nasopharyngeal
carcinoma
(NPC).
The
correlation
between
lncRNAs
expression
NPC
development
has
not
been
well
identified
the
recent
literature.
Recently,
high-through
put
analysis
reveals
LOC100129148
is
highly
expressed
NPC.
However,
whether
aberrant
of
corrected
with
tumorigenesis
or
prognosis
investigated.
Herein,
we
was
up-regulated
tissues
cell
lines,
higher
resulted
a
markedly
poorer
survival
time.
Over-expressed
favored,
but
silenced
hampered
proliferation
cells.
Additionally,
enhanced
KLF12
through
functioning
as
competitive
âspongeâ
for
miR-539-5p.
Thus,
our
study
reports
novel
mechanism
underlying
carcinogenesis,
provides
potential
diagnosis
treatment
biomarker
Journal of Cellular Physiology,
Journal Year:
2018,
Volume and Issue:
234(6), P. 9408 - 9416
Published: Nov. 14, 2018
Long
noncoding
RNA
(lncRNA)
differentiation
antagonizing
nonprotein
coding
(DANCR)
has
been
identified
as
an
oncogene
in
several
cancers.
However,
the
biological
function
and
role
of
DANCR
hepatocellular
carcinoma
(HCC)
remain
unclear.
Our
current
study
aimed
to
investigate
detailed
mechanism
HCC.
We
found
that
was
significantly
upregulated
HCC
cell
lines
comparison
LO2
cells.
Then,
we
observed
knockdown
could
greatly
inhibit
Huh7
HepG2
proliferation.
In
addition,
apoptosis
increased
by
silence
meanwhile,
cycle
progression
blocked
G1
phase.
Apart
from
these,
downregulation
repressed
migration
invasion
ability
obviously.
As
predicted
bioinformatics
analysis,
microRNA-216a-5p
(miR-216a-5p)
serve
a
direct
target
DANCR.
MiR-216a-5p
reported
be
involved
many
Here,
correlation
between
miR-216a-5p
confirmed
using
dual-luciferase
reporter
assay
radioimmunoprecipitation
assay.
Subsequently,
Kruppel-like
factor
12
(KLF12)
exerts
important
different
tumor
types.
KLF12
can
downstream
miR-216a-5p.
Finally,
vivo
experiments
were
used
data
proved
also
strongly
suppressed
growth
via
targeting
KLF12.
conclusion,
our
indicated
might
provide
new
perspective
for
treatment.
Biomedicine & Pharmacotherapy,
Journal Year:
2020,
Volume and Issue:
125, P. 109865 - 109865
Published: Feb. 12, 2020
The
pathogenesis
of
ovarian
cancer
remains
to
be
elucidated.
Our
previous
study
demonstrated
that
myosin
heavy
chain
9
(MYH9)
overexpression
was
associated
with
poor
prognosis
epithelial
cancer.
However,
the
mechanism
MYH9
and
its
regulation
by
microRNA
(miR)
is
not
clear.
results
present
miR-6089
one
microRNAs
targeting
MYH9,
suppressed
cell
proliferation,
migration,
invasion
metastasis
in
vivo
vitro.
Mechanistic
studies
confirmed
directly
targeted
inactivate
Wnt/β-catenin
signalling
pathway
downstream
epithelial-to-mesenchymal
transition
(EMT),
cell-cycle
factors
c-Jun,
whereas
reversed
inhibitory
effects
cells
upregulating
EMT,
c-Jun.
Interestingly,
transcriptionally
inhibited
a
transcription
factor
which
could
induced
via
pathway.
Thus
miR-6089/MYH9/β-catenin/c-Jun
formed
negative
feedback
loop
In
clinical
samples,
negatively
correlated
expression.
first
demonstrate
serves
as
tumor-suppressive
miRNA,
inhibits
carcinogenesis
progression.
