iLiver, Journal Year: 2025, Volume and Issue: unknown, P. 100146 - 100146
Published: Feb. 1, 2025
Language: Английский
Drug Resistance Updates, Journal Year: 2023, Volume and Issue: 68, P. 100938 - 100938
Published: Feb. 9, 2023
Language: Английский
Citations
98
Journal of Experimental & Clinical Cancer Research, Journal Year: 2023, Volume and Issue: 42(1)
Published: July 18, 2023
Cancer is the main cause of death worldwide and metastasis a major poor prognosis cancer-associated mortality. Metastatic conversion cancer cells multiplex process, including EMT through cytoskeleton remodeling interaction with TME. Tens thousands putative lncRNAs have been identified, but biological functions most are still to be identified. However, already emerged as key regulators gene expression at transcriptional post-transcriptional level control in spatio-temporal fashion. LncRNA-dependent mechanisms can cell fates during development their perturbed associated onset progression many diseases cancer. LncRNAs involved each step different modes action. The investigation roles could possibly lead identification new biomarkers innovative therapeutic options.
Language: Английский
Citations
60
Journal of Neuroinflammation, Journal Year: 2022, Volume and Issue: 19(1)
Published: July 12, 2022
Abstract Background Studies have suggested that many down-regulated miRNAs identified in the brain tissue or serum of Alzheimer’s disease (AD) patients were involved formation senile plaques and neurofibrillary tangles. Specifically, our previous study revealed microRNA-22-3p (miR-22-3p) was significantly AD patients. However, molecular mechanism underlying down-regulation miR-22-3p has not been comprehensively investigated. Methods The ameliorating effect on apoptosis Aβ-treated HT22 cells detected by TUNEL staining, flow cytometry, western blotting. cognition mice with stereotaxic injection agomir antagomir assessed Morris water maze test. Pathological changes mouse hippocampus analyzed using hematoxylin eosin (HE) Nissl immunohistochemistry. Proteomics analysis performed to identify targets miR-22-3p, which further validated dual-luciferase reporter blotting analysis. Results played an important role cells. Increased levels improved mice. Although did cause decrease neuronal loss hippocampus, it reduced Aβ deposition. Sox9 protein as target verified luciferase experiments. Conclusion Our showed could improve reduce deposition acting through NF-κB signaling pathway We concluded ameliorated targeting hippocampus.
Language: Английский
Citations
45
Journal of Experimental & Clinical Cancer Research, Journal Year: 2023, Volume and Issue: 42(1)
Published: Oct. 26, 2023
Lung cancer is the most common and deadliest worldwide, approximately 90% of all lung deaths are caused by tumor metastasis. Tumor-derived exosomes could potentially promote metastasis through delivery metastasis-related molecules. However, function underlying mechanism exosomal long noncoding RNA (lncRNA) in remain largely unclear.Cell were purified from conditioned media differential ultracentrifugation observed using transmission electron microscopy, size distributions determined nanoparticle tracking analysis. Exosomal lncRNA sequencing (lncRNA-seq) was used to identify RNAs. Cell migration invasion wound-healing assays, two-chamber transwell assays cell mobility tracking. Mice orthotopically subcutaneously xenografted with human cells evaluate vivo. Western blot, qRT‒PCR, RNA-seq, dual-luciferase reporter performed investigate potential mechanism. The level plasma examined qRT‒PCR. MS2-tagged affinity purification (MS2-TRAP) verify lncRNA-bound miRNAs.Exosomes derived highly metastatic promoted low potential. Using lncRNA-seq, we found that a novel lncRNA, lnc-MLETA1, upregulated their secreted exosomes. Overexpression lnc-MLETA1 augmented cancer. Conversely, knockdown attenuated motility cells. Interestingly, exosome-transmitted Reciprocally, targeting an LNA suppressed exosome-induced motility. Mechanistically, regulated expression EGFR IGF1R sponging miR-186-5p miR-497-5p facilitate clinical datasets revealed tissues predicts survival patients. Importantly, levels positively correlated patients.This study identifies as critical mediates crosstalk may serve prognostic biomarker therapeutic target for diagnosis treatment.
Language: Английский
Citations
28
Molecular Cancer, Journal Year: 2023, Volume and Issue: 22(1)
Published: July 22, 2023
Abstract Background The encapsulation of circular RNAs (circRNAs) into extracellular vesicles (EVs) enables their involvement in intercellular communication and exerts an influence on the malignant advancement various tumors. However, regulatory role EVs-circRNA renal cell carcinoma (RCC) remains elusive. Methods vitro vivo functional experiments were implemented to measure effects circEHD2 phenotype RCC. EVs-circEHD2 activation fibroblasts was assessed by collagen contraction assay, western blotting, enzyme-linked immunosorbent assay (ELISA). mechanism investigated RNA pull-down immunoprecipitation, chromatin isolation purification, luciferase co-immunoprecipitation assay. Results We demonstrated that upregulated RCC tissues serum EVs patients with metastasis. Silencing inhibited tumor growth vivo. Mechanistic studies indicated FUS -binding protein (FUS) accelerated cyclization circEHD2, then interacts tyrosine 3-monooxygenase/tryptophan 5-monooxygenase eta (YWHAH), which acts as a bridge recruit Yes1-associated transcriptional regulator (YAP) promoter SRY-box transcription factor 9 (SOX9); this results sustained SOX9. Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2B1) regulates package EVs, transmits fibroblasts, converting cancer-associated (CAFs). Activated CAFs promote metastasis secreting pro-inflammatory cytokines such IL-6. Furthermore, antisense oligonucleotides (ASOs) targeting exhibited strong inhibition Conclusions circEHD2/YWHAH/YAP/SOX9 signaling pathway accelerates facilitates CAFs. Our suggest may be useful biomarker therapeutic target for
Language: Английский
Citations
27
Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)
Published: Dec. 8, 2023
Abstract Gastric cancer (GC) is a heterogeneous disease, threatening millions of lives worldwide, yet the functional roles long non-coding RNAs (lncRNAs) in different GC subtypes remain poorly characterized. Microsatellite stable (MSS)/epithelial-mesenchymal transition (EMT) most aggressive subtype associated with poor prognosis. Here, we apply integrated network analysis to uncover lncRNA heterogeneity between subtypes, and identify MIR200CHG as master regulator mediating EMT specifically MSS/EMT GC. The expression silenced by promoter hypermethylation, reverses mesenchymal identity cells vitro inhibits metastasis vivo. Mechanistically, not only facilitates biogenesis its intronic miRNAs miR-200c miR-141, but also protects from target-directed miRNA degradation (TDMD) through direct binding miR-200c. Our studies reveal landscape subtype-specific regulatory network, providing clinically relevant biological insights towards
Language: Английский
Citations
27
Molecular Cancer, Journal Year: 2024, Volume and Issue: 23(1)
Published: Sept. 30, 2024
Language: Английский
Citations
12
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: May 2, 2024
Detecting early-stage esophageal squamous cell carcinoma (ESCC) and precancerous lesions is critical for improving survival. Here, we conduct whole-genome bisulfite sequencing (WGBS) on 460 cfDNA samples from patients with non-metastatic ESCC or matched healthy controls. We develop an expanded multimodal analysis (EMMA) framework to simultaneously identify methylation, copy number variants (CNVs), fragmentation markers in WGBS data. methylation are the earliest most sensitive, detectable 70% of ESCCs 50% lesions, associated molecular subtypes tumor microenvironments. CNVs features show high specificity but linked late-stage disease. EMMA significantly improves detection rates, increasing AUCs 0.90 0.99, detects 87% 62% >95% validation cohorts. Our findings demonstrate potential methylome early monitoring characteristics ESCC.
Language: Английский
Citations
11
Molecular Medicine Reports, Journal Year: 2024, Volume and Issue: 29(6)
Published: April 5, 2024
Emerging scientific evidence has suggested that the long non‑coding (lnc)RNA differentiation antagonizing non‑protein coding RNA (
Language: Английский
Citations
10
Cancer Science, Journal Year: 2024, Volume and Issue: 115(7), P. 2269 - 2285
Published: May 8, 2024
Dysregulation of long noncoding RNA (lncRNA) expression plays a pivotal role in the initiation and progression gastric cancer (GC). However, regulation lncRNA SNHG15 GC has not been well studied. Mechanisms for ferroptosis by have revealed. Here, we aimed to explore SNHG15-mediated biological functions underlying molecular mechanisms GC. The novel was identified analyzing RNA-sequencing (RNA-seq) data tissues from our cohort TCGA dataset, further validated qRT-PCR cells tissues. Gain- loss-of-function assays were performed examine on both vitro vivo. highly expressed enhanced positively correlated with malignant stage poor prognosis patients. studies showed that required affect cell growth, migration invasion Mechanistically, oncogenic transcription factors E2F1 MYC could bind promoter enhance its expression. Meanwhile, increased mRNA sponging miR-24-3p. Notably, also stability SLC7A11 cytoplasm competitively binding HNRNPA1. In addition, inhibited through an HNRNPA1-dependent SLC7A11/GPX4 axis. Our results support model which E2F1- MYC-activated regulates via modulation axis, serves as critical effectors progression, provides new therapeutic direction treatment
Language: Английский
Citations
10