HNRNPC as a pan-cancer biomarker and therapeutic target involved in tumor progression and immune regulation DOI
Yuezhou Zhang, Zhao Zhang, Jianzeng Dong

et al.

Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics, Journal Year: 2024, Volume and Issue: 33(1), P. 83 - 102

Published: Nov. 20, 2024

Aberrant expression of RNA-binding proteins (RBPs) has been linked to a variety diseases, including hematological disorders, cardiovascular and multiple types cancer. Heterogeneous nuclear ribonucleoprotein C (HNRNPC), member belonging the heterogeneous (hnRNP) family, plays pivotal role in nucleic acid metabolism. Previous studies have underscored significance HNRNPC tumorigenesis; however, its specific malignant tumor progression remains inadequately characterized. We leveraged publicly available databases, The Cancer Genome Atlas (TCGA), explore potential involvement across various cancers. Additionally, we performed experimental validation focused on liver Our analysis revealed that is overexpressed wide range common malignancies, lung cancers, strongly unfavorable outcomes. Furthermore, was observed be closely immunity. Through immune checkpoint cell infiltration assessment, emerged as target for modulating immunotherapy. Notably, silencing markedly inhibited proliferation, metastasis, cancer cells. In summary, our findings highlight prognostic marker cancer, suggest shaping microenvironment. These insights offer avenues improving clinical outcomes tumors with elevated expression, particularly through immunotherapeutic strategies.

Language: Английский

Single-cell transcriptome analysis reveals the association between histone lactylation and cisplatin resistance in bladder cancer DOI
Fei Li, Henghui Zhang, Yu‐An Huang

et al.

Drug Resistance Updates, Journal Year: 2024, Volume and Issue: 73, P. 101059 - 101059

Published: Jan. 19, 2024

Language: Английский

Citations

50
circASAP1 induces renal clear cell carcinoma ferroptosis by binding to HNRNPC and thereby regulating GPX4 DOI Creative Commons
Yunfei Wang,

Taowei Yang,

Qihao Li

et al.

Molecular Cancer, Journal Year: 2025, Volume and Issue: 24(1)

Published: Jan. 2, 2025

Clear cell renal carcinoma (ccRCC) represents the most prevalent subtype, accounting for nearly 80% of all RCC cases. Recent research has shown that high expression circular non-coding RNA (circRNA) is associated with poor prognosis in patients clear (ccRCC), however, underlying mechanism remains unclear. After analysing self-sequenced cancer and paracancer circRNA sequencing data comparing it GEO public database, we discovered circASAP1 was significantly up-regulated cancers. We also tested levels 102 found metastasis. The interaction between circASAP1, HNRNPC their downstream target genes confirmed through experiments such as pull-down, RIP fluorescence situ hybridisation. A series vitro vivo functional were performed to verify effects on proliferation Circular analysis revealed markedly elevated ccRCC, a significant association observed Actinomycin D, RNase R, well hybridization (FISH) analyses ring structure cytoplasmic localization circASAP1. High ccRCC proliferative viability, invasion, metastasis CCK-8, transwell, plate cloning, EdU experiments. Interaction by immunoprecipitation, FISH, silver staining, mass spectrometry. Experiments using truncated isoforms demonstrated amino acids 16–87 bound Proteins altered enriched ferroptosis pathway Kyoto Encyclopedia Genes Genomes enrichment analysis. relationship ASAP1/HNRNPC/GPX4 axis experimental data, which further rescue influenced tumor growth animal predicted ccRCC. circASAP1/HNRNPC/GPX4 provides novel directions potential targets treatment.

Language: Английский

Citations

2
Neoantigens in cancer immunotherapy: focusing on alternative splicing DOI Creative Commons
Peng Huang,

Feng Wen,

Nuerye Tuerhong

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: July 11, 2024

Alternative splicing (AS) functions as a crucial program in transcriptional modulation, leading to proteomic diversity and functional alterations of proteins. These actions induce various neoantigens that hold prognostic significance contribute aspects cancer progression, including immune responses against cancer. The advent immunotherapy has remarkably revolutionized tumor therapy. In this regard, AS-derived are potent targets for vaccines chimeric antigen receptor (CAR) T cell therapies. review, we outline serve promising immunotherapeutic guide strategies. This evidence contributes deeper comprehension the complexity provides novel perspectives techniques precision medicine immunotherapy. Moreover, underscore obstacles awaited be addressed approach become clinically applicable.

Language: Английский

Citations

9
Role of the circRNA/miRNA/mRNA axis in Renal Cell Carcinoma: from gene regulation to metabolism and immunity DOI Creative Commons

Zhengjun Xu,

Yurong Dong,

Kun Cui

et al.

iScience, Journal Year: 2025, Volume and Issue: 28(4), P. 112183 - 112183

Published: March 12, 2025

Renal cell carcinoma (RCC), particularly clear RCC (ccRCC), has seen rising incidence and mortality rates. Early detection remains difficult due to nonspecific symptoms, often leading diagnoses at advanced stages unfavorable prognoses. Current treatments, including surgery, targeted therapies, immunotherapies, face limitations such as adverse reactions, drug resistance, immune evasion. This review discusses the role of circular RNAs (circRNAs) their involvement in circRNA/miRNA/mRNA axis during progression. As competitive endogenous (ceRNAs), circRNAs regulate gene expression cellular processes, proliferation, metastasis, apoptosis, resistance. Furthermore, we explore impact on tumor metabolic reprogramming influence microenvironment. We also summarize clinical prospects, challenges, future directions this field. A comprehensive understanding these complex interactions may provide new insights into diagnosis treatment RCC, ultimately overcoming existing challenges improving patient outcomes.

Language: Английский

Citations

0
O-GlcNAcylation regulation of RIPK1-dependent apoptosis dictates sensitivity to sunitinib in renal cell carcinoma DOI
Xiangbo Zeng, Zhiliang Chen, Yuanchao Zhu

et al.

Drug Resistance Updates, Journal Year: 2024, Volume and Issue: 77, P. 101150 - 101150

Published: Sept. 12, 2024

Language: Английский

Citations

4
The role of ubiquitination in health and disease DOI Creative Commons

Yan Liao,

Wangzheqi Zhang,

Yang Liu

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(10)

Published: Sept. 25, 2024

Ubiquitination is an enzymatic process characterized by the covalent attachment of ubiquitin to target proteins, thereby modulating their degradation, transportation, and signal transduction. By precisely regulating protein quality quantity, ubiquitination essential for maintaining homeostasis, DNA repair, cell cycle regulation, immune responses. Nevertheless, diversity enzymes extensive involvement in numerous biological processes contribute complexity variety diseases resulting from dysregulation. The relies on a sophisticated system, domains, receptors, which collectively impart versatility pathway. widespread presence highlights its potential induce pathological conditions. Ubiquitinated proteins are predominantly degraded through proteasomal also plays key role localization transport, as well inflammatory pathways. This review systematically delineates roles genomic stability, cellular proliferation, Furthermore, mechanisms implicated various pathologies, alongside current modulators discussed. Enhancing our comprehension aims provide novel insights into involving propose innovative therapeutic strategies clinical

Language: Английский

Citations

4
Monotropein attenuates renal cell carcinoma cell progression and M2 macrophage polarization by weakening NF-κB DOI
Heping Qiu, Fei Liu, Mei Qiu

et al.

International Urology and Nephrology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Language: Английский

Citations

0
5-Methylcytosine-modified circRNA-CCNL2 regulates vascular remdeling in hypoxic pulmonary hypertension through binding to FXR2 DOI

Hanliang Sun,

Xiudong Liao, Shanshan Wang

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 296, P. 139638 - 139638

Published: Jan. 11, 2025

Language: Английский

Citations

0
Heme-thiolate monooxygenase cytochrome P450 1B1, an old dog with many new tricks DOI
Jong-Won Kim, Hung‐Chun Tung, Bin Yang

et al.

Pharmacological Reviews, Journal Year: 2025, Volume and Issue: 77(3), P. 100045 - 100045

Published: Feb. 5, 2025

Cytochrome P450 CYP1B1 is a heme-thiolate monooxygenase traditionally recognized for its xenobiotic functions and extrahepatic expressions. Recent studies have suggested that also expressed in hepatic stellate cells, immune endothelial fibroblasts within the tumor microenvironment, as well cells themselves. responsible metabolism of wide range substrates, including xenobiotics such drugs, environmental chemicals, endobiotics steroids, retinol, fatty acids. Consequently, associated exogenous endogenous metabolites been critically implicated pathogenesis many diseases. Understanding mode action different pathophysiological conditions developing pharmacological inhibitors allow systemic or cell type-specific modulation may pave way novel therapeutic opportunities. This review highlights significant role maintaining physiological homeostasis provides comprehensive discussion recent advancements our understanding CYP1B1's involvement diseases fibrosis, cancer, glaucoma, metabolic disorders. Finally, emphasizes potential targeting drug development, particularly treatment prevention cancers liver fibrosis. SIGNIFICANCE STATEMENT: plays critical various processes. Dysregulation genetic mutations gene encoding this enzyme can lead to health complications increase risk cancer In review, we summarize preclinical clinical evidence underscores target.

Language: Английский

Citations

0
Targeting m6A RNA Modification in Tumor Therapeutics DOI Creative Commons
Zhenwei Mao, Min Li, Shengjun Wang

et al.

Current Oncology, Journal Year: 2025, Volume and Issue: 32(3), P. 159 - 159

Published: March 11, 2025

The prevalent eukaryotic RNA modification N6-methyladenosine (m6A), which is distributed in more than 50% of cases, has demonstrated significant implications both normal development and disease progression, particularly the context cancer. This review aims to discuss potential efficacy targeting tumor cells through modulation m6A levels. Specifically, we how upregulation or downregulation integral specific targets effective treating different types patients. Additionally, will cover factors influencing treatment. Our focus on impact mRNA genes assess its as a therapeutic strategy for tumors. Despite challenges involved, further research tumors integration with existing therapy approaches warranted.

Language: Английский

Citations

0