Nanotechnology in Gene Editing: Pioneering CRISPR‐Cas Delivery Systems to Tackle Antibiotic Resistance DOI Open Access
Sahar Gholamian,

Pooya Baghaee,

Mohammad Doroudian

et al.

Advanced Therapeutics, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

Abstract The rise of antibiotic‐resistant bacteria, driven by antibiotic misuse, is a major global health threat. Addressing this issue requires understanding resistance mechanisms and developing innovative solutions. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)‐associated systems (Cas), genome‐editing tool derived from prokaryotic defense mechanisms, offers precise targeting genes. By reprogramming CRISPR‐Cas, bacteria can be killed or resensitized to antibiotics through plasmid curing. However, clinical applications face challenges, particularly in delivering CRISPR‐Cas components effectively. Nanotechnology has emerged as promising approach for targeted delivery tissues cells. This paper explores the molecular resistance, emphasizing structure function their mechanisms. It highlights use nanoparticles (NPs) nanoscale carriers deliver components, reviewing recent studies that combine NPs CRISPR target Additionally, discusses current challenges future prospects field, underscoring potential nanotechnology combat resistance.

Language: Английский

tRNA-derived small RNAs in human cancers: roles, mechanisms, and clinical application DOI Creative Commons

Manli Zhou,

Xiaoyun He, Jing Zhang

et al.

Molecular Cancer, Journal Year: 2024, Volume and Issue: 23(1)

Published: April 15, 2024

Abstract Transfer RNA (tRNA)-derived small RNAs (tsRNAs) are a new type of non-coding (ncRNAs) produced by the specific cleavage precursor or mature tRNAs. tsRNAs involved in various basic biological processes such as epigenetic, transcriptional, post-transcriptional, and translation regulation, thereby affecting occurrence development human diseases, including cancers. Recent studies have shown that play an important role tumorigenesis regulating behaviors malignant proliferation, invasion metastasis, angiogenesis, immune response, tumor resistance, metabolism reprogramming. These may be potential targets for treatment. Furthermore, can exist abundantly stably bodily fluids (e.g., blood, serum, urine) form free encapsulated extracellular vesicles, intercellular communication microenvironment (TME). Meanwhile, their abnormal expression is closely related to clinicopathological features patients, staging, lymph node poor prognosis patients; thus, served novel liquid biopsy biomarker. This review summarizes discovery, production, analyzes molecular mechanisms applications therapy, which provide strategies early diagnosis targeted therapy tumors.

Language: Английский

Citations

19
Bioengineering in Solid-State Fermentation for next sustainable food bioprocessing DOI Creative Commons
Muyideen Olaitan Bamidele, Micheal Bola Bamikale, Eliseo Cárdenas-Hernández

et al.

Next Sustainability, Journal Year: 2025, Volume and Issue: 6, P. 100105 - 100105

Published: Jan. 1, 2025

Language: Английский

Citations

5
Current Advances in the Therapeutic Potential of Scutellarin: Novel Applications, Mechanisms, and Future Challenges. DOI Creative Commons
Great Iruoghene Edo, Alice Njolke Mafe, Patrick Othuke Akpoghelie

et al.

Phytomedicine Plus, Journal Year: 2025, Volume and Issue: unknown, P. 100754 - 100754

Published: Jan. 1, 2025

Language: Английский

Citations

2
Optimizing CRISPR/Cas9 precision: Mitigating off-target effects for safe integration with photodynamic and stem cell therapies in cancer treatment DOI Open Access

J. P. Jose Merlin,

Heidi Abrahamse

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 180, P. 117516 - 117516

Published: Sept. 26, 2024

Language: Английский

Citations

11
Leveraging CRISPR gene editing technology to optimize the efficacy, safety and accessibility of CAR T-cell therapy DOI Creative Commons
Tao Lei, Yazhuo Wang, Yuchen Zhang

et al.

Leukemia, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 25, 2024

Language: Английский

Citations

11
Biotechnological Tools Boost the Functional Diversity of DNA-based Data Storage Systems DOI Creative Commons
Xiaoyuan Xu, Wen Wang, Zhi Ping

et al.

Computational and Structural Biotechnology Journal, Journal Year: 2025, Volume and Issue: 27, P. 624 - 630

Published: Jan. 1, 2025

DNA-based data storage has emerged as a groundbreaking solution to the growing demand for efficient, high-density, and long-term storage. It is attracting many researchers' attention, who are implementing functions such random access, searching, operations apart from existing capabilities, including reading writing. We summarize recent progress of how biotechnological tools, based on sequence specificity, encapsulation, high-dimensional structures DNA molecules, facilitate implementation various functions. The limitations using biochemical reactions that hinder development more precise efficient information systems also discussed. Future advancements in molecular biology nanotechnology expected improve architecture, scalability, efficiency storage, positioning it sustainable dynamic alternative conventional systems.

Language: Английский

Citations

1
Advances in delivery systems for CRISPR/Cas-mediated cancer treatment: a focus on viral vectors and extracellular vesicles DOI Creative Commons

Zhidu Song,

Tao Ying,

Yue Liu

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 30, 2024

The delivery of CRISPR/Cas systems holds immense potential for revolutionizing cancer treatment, with recent advancements focusing on extracellular vesicles (EVs) and viral vectors. EVs, particularly exosomes, offer promising opportunities targeted therapy due to their natural cargo transport capabilities. Engineered EVs have shown efficacy in delivering components tumor cells, resulting inhibited cell proliferation enhanced chemotherapy sensitivity. However, challenges such as off-target effects immune responses remain significant hurdles. Viral vectors, including adeno-associated viruses (AAVs) adenoviral vectors (AdVs), represent robust platforms systems. AAVs, known safety profile, already been employed clinical trials gene therapy, demonstrating treatment. AdVs, capable infecting both dividing non-dividing versatility disease modeling drug discovery. Despite efficacy, present several challenges, effects. Future directions entail refining enhance specificity minimize adverse effects, heralding personalized effective CRISPR/Cas-mediated therapies. This article underscores the importance optimized mechanisms realizing full therapeutic technology oncology. As field progresses, addressing these will be pivotal translating treatments from bench bedside.

Language: Английский

Citations

7
Correction: Comprehensive review of CRISPR‑based gene editing: mechanisms, challenges, and applications in cancer therapy DOI Creative Commons
Mohammad Chehelgerdi, Matin Chehelgerdi, Milad Khorramian‐Ghahfarokhi

et al.

Molecular Cancer, Journal Year: 2024, Volume and Issue: 23(1)

Published: Feb. 27, 2024

s attention that this article cites their work in an incorrect fashion and at least the related part of paper raises some concern about integrity reported information.In Table 3 on clinical trials CRISPR-based therapy manuscript, authors cite our study (Ref

Language: Английский

Citations

6
Epigenetic deregulation in breast cancer microenvironment: Implications for tumor progression and therapeutic strategies DOI Creative Commons
Lenka Trnkova, Verona Buociková, Michal Mego

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 174, P. 116559 - 116559

Published: April 10, 2024

Breast cancer comprises a substantial proportion of diagnoses in women and is primary cause cancer-related mortality. While hormone-responsive cases generally have favorable prognosis, the aggressive nature triple-negative breast presents challenges, with intrinsic resistance to established treatments being persistent issue. The complexity intensifies emergence acquired resistance, further complicating management cancer. Epigenetic changes, encompassing DNA methylation, histone RNA modifications, non-coding RNAs, are acknowledged as crucial contributors heterogeneity unique epigenetic landscape harbored by each cellular component within tumor microenvironment (TME) adds great diversity intricate regulations which influence therapeutic responses. TME, sophisticated ecosystem non-cellular elements interacting cells, establishes an immunosuppressive fuels processes such growth, angiogenesis, extracellular matrix remodeling. These factors contribute challenging conditions treatment fostering hypoxic environment, inducing metabolic stress, creating physical barriers drug delivery. This article delves into complex connections between response, underlying vital interactions TME. To restore sensitivity treatment, it emphasizes need for combination therapies considering changes specific individual members Recognizing pivotal role epigenetics comprehending specificities TME essential devising more effective strategies. development reliable biomarkers patient stratification will facilitate tailored precise approaches.

Language: Английский

Citations

6
Immunometabolic reprogramming of macrophages with inhalable CRISPR/Cas9 nanotherapeutics for acute lung injury intervention DOI

Wanling Huang,

Gaohong Fu,

Yangeng Wang

et al.

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 181, P. 308 - 316

Published: April 1, 2024

Language: Английский

Citations

5