Myofibroblast-derived extracellular vesicles facilitate cancer stemness of hepatocellular carcinoma via transferring ITGA5 to tumor cells DOI Creative Commons
Xiao Yang, Ping Tao, Keke Zhang

et al.

Molecular Cancer, Journal Year: 2024, Volume and Issue: 23(1)

Published: Nov. 21, 2024

Myofibroblasts constitute a significant component of the tumor microenvironment (TME) and play pivotal role in progression hepatocellular carcinoma (HCC). Integrin α5 (ITGA5) is crucial regulator myofibroblasts malignant tumors. Therefore, potential ITGA5 as novel target for therapeutic strategy HCC should be investigated. Digital scanning analysis tissue microarray were performed to locate distribution conduct prognosis analysis. CRISPR Cas9-mediated knockout was establish ITGA5-KO myofibroblast cell line. Extracellular vesicles (EVs) derived from LX2 extracted treatment cells. Subsequently, sphere-forming ability stemness markers expression treated cells examined. An orthotopic mouse model with fibrotic injury constructed test outcomes ITGA5-targeting therapy its efficacy programmed death-ligand 1 (PD-L1) treatment. Co-immunoprecipitation/mass spectrometry transcriptome data integrated delve into mechanism. The results revealed that highly enriched stromal tissues contributed enhanced poor prognosis. Notably, transmission via extracellular induced acquisition cancer stem cell-like properties. Mechanistically, directly bind YES1, facilitating activation YES1 downstream pathways, thereby enhancing Furthermore, blockade impeded driven by ITGA5+ PD-L1 HCC. Our findings elucidated mechanism which EV-mediated transfer augmented stemness. helped prevent improved

Language: Английский

Tumor microenvironment targeted nano-drug delivery systems for multidrug resistant tumor therapy DOI Creative Commons

Xinyue Shao,

Xiaoling Zhao, Binghao Wang

et al.

Theranostics, Journal Year: 2025, Volume and Issue: 15(5), P. 1689 - 1714

Published: Jan. 2, 2025

In recent years, nano-drug delivery systems (Nano-DDS) that target the tumor microenvironment (TME) to overcome multidrug resistance (MDR) have become a research hotspot in field of cancer therapy. By precisely targeting TME and regulating its unique pathological features, such as hypoxia, weakly acidic pH, abnormally expressed proteins, etc., these Nano-DDS enable effective therapeutic agents reversal MDR. This scientific community is increasing investment development diversified exploring their anti-drug potential. Therefore, it particularly important conduct comprehensive review progress TME-targeted years. After brief introduction MDR, design principle structure liposomes, polymer micelles inorganic nanocarriers are focused on, characteristics described. It also demonstrates how break through MDR treatment various mechanisms, discusses synthetic innovation, results overcoming mechanisms. The was concluded with deliberations on key challenges future outlooks

Language: Английский

Citations

4
Comprehensive review of drug resistance in mammalian cancer stem cells: implications for cancer therapy DOI Creative Commons

Bemrew Admassu Mengistu,

Tirunesh Tsegaw,

Yitayew Demessie

et al.

Cancer Cell International, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 18, 2024

Cancer remains a significant global challenge, and despite the numerous strategies developed to advance cancer therapy, an effective cure for metastatic elusive. A major hurdle in treatment success is ability of cells, particularly stem cells (CSCs), resist therapy. These CSCs possess unique abilities, including self-renewal, differentiation, repair, which drive tumor progression chemotherapy resistance. The resilience linked certain signaling pathways. Tumors with pathway-dependent often develop genetic resistance, whereas those pathway-independent undergo epigenetic changes that affect gene regulation. can evade cytotoxic drugs, radiation, apoptosis by increasing drug efflux transporter activity activating survival mechanisms. Future research should prioritize identification new biomarkers molecules better understand use cutting-edge approaches, such as bioinformatics, genomics, proteomics, nanotechnology, offers potential solutions this challenge. Key include developing targeted therapies, employing nanocarriers precise delivery, focusing on CSC-targeted pathways Wnt, Notch, Hedgehog Additionally, investigating multitarget inhibitors, immunotherapy, nanodrug delivery systems critical overcoming resistance cells.

Language: Английский

Citations

6
Cellular plasticity and non-small cell lung cancer: role of T and NK cell immune evasion and acquisition of resistance to immunotherapies DOI
S Mestiri, Ana Sami, Naresh Sah

et al.

Cancer and Metastasis Reviews, Journal Year: 2025, Volume and Issue: 44(1)

Published: Jan. 25, 2025

Language: Английский

Citations

0
Role of Cancer Associated Fibroblast (CAF) derived miRNAs on head and neck malignancies microenvironment: a systematic review DOI Creative Commons

Parsa Golestannejad,

Mohamad-Parsa Monkaresi,

Farahnaz Zhian Zargaran

et al.

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 1, 2025

MicroRNAs (miRNAs) play a key role in regulating gene expression within the tumor microenvironment, influencing cancer progression and therapy response. Cancer-associated fibroblasts (CAFs) contribute to development by secreting exosomal miRNAs that promote proliferation, invasion, resistance. This systematic review evaluates impact of CAF-derived on head neck malignancies. A search was conducted PubMed, Scopus, WOS, Google Scholar following PRISMA guidelines. Studies focusing cancers were included. Data extraction covered study characteristics, miRNA profiling methods, functional roles, clinical significance. The Scirap tool used for quality assessment. Among 921 identified articles, 21 met inclusion criteria. Findings indicate miR-21-5p, miR-106-5p, miR-196a drive oral squamous cell carcinoma (OSCC), while miR-124 miR-34a-5p act as suppressors. In esophageal (ESCC), miR-21 miR-27a/b chemotherapy resistance, whereas miR-100-5p inhibits lymphangiogenesis. (HNSCC), miR-196b may serve diagnostic biomarkers. Exosomal miR-106a-5p promotes nasopharyngeal (NPC) metastasis, miR-7 resistance (HNC). significantly influence progression, These findings highlight their potential biomarkers therapeutic targets, warranting further research personalized treatment strategies.

Language: Английский

Citations

0
The Role of Monoclonal Antibodies as Therapeutics in HPV-Related Head and Neck Cancers: An Updated Review DOI Creative Commons

Michael Zalin,

Shaan Patel,

Christiana Coggins

et al.

Antibodies, Journal Year: 2025, Volume and Issue: 14(2), P. 37 - 37

Published: April 24, 2025

Background/Objectives: The increasing prevalence of human papillomavirus (HPV)-positive oropharyngeal squamous cell carcinoma (OPSCC) has necessitated a revaluation therapeutic strategies. HPV-driven OPSCC differs from HPV-negative due to its distinct molecular signatures, increased radiosensitivity, and better prognoses. However, despite these differences, treatment strategies have remained largely uniform, resulting in minimal reductions morbidity exposing HPV-positive patients unnecessary toxicity. Monoclonal antibodies (mAbs) become promising option their ability target with fewer systemic side effects. Immune checkpoint inhibitors (ICIs) such as pembrolizumab shown efficacy enhancing the immune response against tumors, while EGFR like cetuximab offer an alternative modality. Current clinical trials aim refine dosing regimens identify combination that may enhance outcomes. Results: Despite evidence, several challenges hinder widespread adoption mAbs standard for practice. This review examines current role treatment, highlighting limitations future research directions. Conclusions: Further studies are needed optimize patient selection, establish standardized protocols, investigate long-term benefits mAb-based therapies this population.

Language: Английский

Citations

0
CAF-mediated tumor vascularization: From mechanistic insights to targeted therapies DOI
Zhi Zhang, Qing Zhang, Yang Wang

et al.

Cellular Signalling, Journal Year: 2025, Volume and Issue: unknown, P. 111827 - 111827

Published: April 1, 2025

Language: Английский

Citations

0
MicroRNAs and lysosomal membrane proteins: Critical interactions in tumor progression and therapy DOI
Jiahao Xu, Shiqiang Liu, Yujie Jin

et al.

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Journal Year: 2025, Volume and Issue: unknown, P. 189303 - 189303

Published: March 1, 2025

Language: Английский

Citations

0
Myofibroblast-derived extracellular vesicles facilitate cancer stemness of hepatocellular carcinoma via transferring ITGA5 to tumor cells DOI Creative Commons
Xiao Yang, Ping Tao, Keke Zhang

et al.

Molecular Cancer, Journal Year: 2024, Volume and Issue: 23(1)

Published: Nov. 21, 2024

Myofibroblasts constitute a significant component of the tumor microenvironment (TME) and play pivotal role in progression hepatocellular carcinoma (HCC). Integrin α5 (ITGA5) is crucial regulator myofibroblasts malignant tumors. Therefore, potential ITGA5 as novel target for therapeutic strategy HCC should be investigated. Digital scanning analysis tissue microarray were performed to locate distribution conduct prognosis analysis. CRISPR Cas9-mediated knockout was establish ITGA5-KO myofibroblast cell line. Extracellular vesicles (EVs) derived from LX2 extracted treatment cells. Subsequently, sphere-forming ability stemness markers expression treated cells examined. An orthotopic mouse model with fibrotic injury constructed test outcomes ITGA5-targeting therapy its efficacy programmed death-ligand 1 (PD-L1) treatment. Co-immunoprecipitation/mass spectrometry transcriptome data integrated delve into mechanism. The results revealed that highly enriched stromal tissues contributed enhanced poor prognosis. Notably, transmission via extracellular induced acquisition cancer stem cell-like properties. Mechanistically, directly bind YES1, facilitating activation YES1 downstream pathways, thereby enhancing Furthermore, blockade impeded driven by ITGA5+ PD-L1 HCC. Our findings elucidated mechanism which EV-mediated transfer augmented stemness. helped prevent improved

Language: Английский

Citations

1