Expression of Fluorescence Reporters and Natural Products in Native Gut Escherichia coli DOI
Dake Liu,

P Ton,

David M. Zong

et al.

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Escherichia coli is a widely studied model organism and an integral component of the human gut microbiome, offering significant potential for bacteria-based therapeutic applications. Despite this promise, engineering native E. strains remains challenging. In study, we employed chassis-independent recombinase-assisted genome (CRAGE) technique to genetically engineer strain EcAZ-1 probiotic Nissle 1917 (EcN). We successfully expressed suite heterologous genes, including bioluminescent lux operon, green fluorescent protein (GFP), oxygen-independent IFP2.0, in both strains. Optimization IFP2.0 fluorescence was achieved under aerobic anaerobic conditions by coexpressing heme oxygenase gene and/or supplementing chromophore biliverdin or hemin. Additionally, engineered these biosynthesize bioactive compounds naringenin mycosporine-like amino acids. This work highlights as versatile platforms synthetic biology, paving way innovative applications biomedical research development.

Language: Английский

Bioactive Bacteria/MOF Hybrids Can Achieve Targeted Synergistic Chemotherapy and Chemodynamic Therapy against Breast Tumors DOI
Jianmei Li, Jie Dai, Ling Zhao

et al.

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(42)

Published: July 3, 2023

Abstract The hypoxic tumor microenvironment (TME) significantly affects cancer treatment. Conventional chemotherapeutic agents cannot effectively target tissue, which decreases efficacy and results in severe toxic side effects. To alleviate this problem, a self‐driving biomotor is developed by functionalizing MCDP nanoparticles containing calcium peroxide doxorubicin (DOX) loaded onto polydopamine‐coated metal–organic frameworks（MOF）, with the anaerobic Bifidobacterium infantis (Bif) for synergistic chemotherapy chemodynamic therapy (CDT) against breast cancer. materials of institute Lavoisier (MIL) frameworks + CaO 2 DOX polydopamine (MCDP)@Bif biohybrid actively targets regions solid tumors via inherent targeting ability Bif. Once it has accumulated generates hydroxyl radicals through enhanced Fenton‐type reactions between Fe 2+ self‐generated hydrogen acidic TME. disruption Ca homeostasis resulting mitochondrial overload triggers apoptosis enhances oxidative stress, promoting cell death. found that concentration MCDP@Bif‐treated 3.8 times higher than free‐DOX‐treated tumors, prolongs median survival tumor‐bearing mice to 69 days reduces effects DOX. Therefore, novel bacteria‐driven drug delivery system highly effective achieving CDT tumors.

Language: Английский

Citations

38
Bacterial‐Mediated Tumor Therapy: Old Treatment in a New Context DOI Creative Commons
Yao Liu, Lili Niu, Nannan Li

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(12)

Published: March 12, 2023

Abstract Targeted therapy and immunotherapy have brought hopes for precision cancer treatment. However, complex physiological barriers tumor immunosuppression result in poor efficacy, side effects, resistance to antitumor therapies. Bacteria‐mediated provides new options address these challenges. Thanks their special characteristics, bacteria excellent ability destroy cells from the inside induce innate adaptive immune responses. Furthermore, bacterial components, including vesicles, spores, toxins, metabolites, other active substances, similarly inherit unique targeting properties capabilities. Bacteria accessory products can even be reprogrammed produce deliver agents according clinical needs. This review first discusses role of different development tumorigenesis latest advances bacteria‐based delivery platforms existing obstacles application. Moreover, prospect challenges transformation engineered are also summarized.

Language: Английский

Citations

31
Towards overcoming obstacles of type II photodynamic therapy: Endogenous production of light, photosensitizer, and oxygen DOI Creative Commons

Lin Yu,

Zhen Liu,

Wei Xu

et al.

Acta Pharmaceutica Sinica B, Journal Year: 2023, Volume and Issue: 14(3), P. 1111 - 1131

Published: Nov. 4, 2023

Conventional photodynamic therapy (PDT) approaches face challenges including limited light penetration, low uptake of photosensitizers by tumors, and lack oxygen in tumor microenvironments. One promising solution is to internally generate light, photosensitizers, oxygen. This can be accomplished through endogenous production, such as using bioluminescence an source, synthesizing genetically encodable

Language: Английский

Citations

29
Cell‐Based Micro/Nano‐Robots for Biomedical Applications: A Review DOI
Bo Chen, Hongyan Sun, Jiaying Zhang

et al.

Small, Journal Year: 2023, Volume and Issue: 20(1)

Published: Aug. 31, 2023

Abstract Micro/nano‐robots are powerful tools for biomedical applications and applied in disease diagnosis, tumor imaging, drug delivery, targeted therapy. Among the various types of micro‐robots, cell‐based micro‐robots exhibit unique properties because their different cell sources. In combination with actuation methods, particularly externally propelled microrobots have enormous potential applications. This review introduces recent progress micro/nano‐robots. Different methods micro/nano‐robots summarized, templates introduced. Furthermore, focuses on precise control using external fields. Potential challenges, further prospects, clinical translations also discussed.

Language: Английский

Citations

25
Doxorubicin-Loaded Microalgal Delivery System for Combined Chemotherapy and Enhanced Photodynamic Therapy of Osteosarcoma DOI

Xueying An,

Danni Zhong,

Wenshu Wu

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(6), P. 6868 - 6878

Published: Jan. 31, 2024

Osteosarcoma (OS) is considered the most frequent type of primary malignant bone tumor. Currently, radiotherapy, photodynamic (PDT), and other therapies for osteosarcoma are limited by tumor hypoxia single efficacy serve side-effects. Herein, we reported a microalgal drug delivery system (SpiD), doxorubicin (DOX)-loaded Spirulina platensis (Spi) OS therapy. The specific surface allowed effective loading DOX via channels electrostatic interactions. Under 650 nm laser irradiation, SpiD enabled high oxygen production photosynthesis enhanced reactive species (ROS) generation chlorophyll-assisted photosensitization, synergistically killing cells with released DOX. Combined chemotherapy PDT mediated exerted synergic antitumor effects resulted in potent therapeutic orthotopic mice. Furthermore, could reduce side-effects chemotherapy, showing excellent blood tissue safety. Taken together, this provided natural, efficient, safe, inexpensive strategy treatment.

Language: Английский

Citations

10
Programmable Bacteria‐Based Biohybrids as Living Biotherapeutics for Enhanced Cancer Sonodynamic‐Immunotherapy DOI
Cheng Wang, Linfu Chen, Jiafei Zhu

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(30)

Published: April 9, 2024

Abstract Synthetic biology is propelling medicine into a new era through its capacity to genetically program living cells. One of the particular interests engineering bacteria as live and targeted therapeutic delivery system. Herein, bacterial biohybrid ( E. coli ‐pE@PCN) developed by Escherichia BL21 overexpress catalase ‐pE) electrostatically adsorbing nano‐sonosensitizers (PCN NPs) for enhanced sonodynamic therapy (SDT). Leveraging ability colonize penetrate deep in tumors, engineered can not only sustainably express relieve tumor hypoxia, but also facilitate enriched expanded distribution carried sonosensitizer at site, so trigger effective SDT. More interestingly, it found that ‐pE@PCN‐based SDT successfully inhibit growth subcutaneous orthotopic colorectal tumors inducing potent antitumor immune responses due released tumor‐associated antigens native immunogenicity pathogen‐associated molecular patterns. Furthermore, prime strong memory response prevent recurrence elicit abscopal effect metastasis. Therefore, programmable bacteria‐based biohybrids here pave an avenue prepare next‐generation sonodynamic‐immunotherapeutics eliminate cancer relapse

Language: Английский

Citations

10
Genetically engineered bacteria-mediated multi-functional nanoparticles for synergistic tumor-targeting therapy DOI

Yaotai Wang,

Yu Tang, Yan Du

et al.

Acta Biomaterialia, Journal Year: 2022, Volume and Issue: 150, P. 337 - 352

Published: Aug. 2, 2022

Language: Английский

Citations

29
Bacteria-Based Nanoprobes for Cancer Therapy DOI Creative Commons
Yiping Lu, Nan Mei,

Yinwei Ying

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 759 - 785

Published: Jan. 1, 2024

Abstract: Surgical removal together with chemotherapy and radiotherapy has used to be the pillars of cancer treatment. Although these traditional methods are still considered as first-line or standard treatments, non-operative situation, systemic toxicity resistance severely weakened therapeutic effect. More recently, synthetic biological nanocarriers elicited substantial interest exhibited promising potential for combating cancer. In particular, bacteria their derivatives omnipotent realize intrinsic tumor targeting inhibit growth anti-cancer agents secreted immune response. They frequently employed in synergistic bacteria-mediated anticancer treatments strengthen effectiveness this review, we elaborate on development, mechanism advantage bacterial therapy against then systematically introduce bacteria-based nanoprobes recent achievements treatment strategies clinical trials. We also discuss advantages well limitations nanoprobes, especially questions that hinder application human, exhibiting novel endeavor comprehensively. Keywords: nanoprobe, therapy, nanomedicine, targeting, microenvironment, trial

Language: Английский

Citations

7
Insights into the enigma of oral streptococci in carcinogenesis DOI

Sangeetha Senthil Kumar,

Michael D. L. Johnson, Justin E. Wilson

et al.

Microbiology and Molecular Biology Reviews, Journal Year: 2024, Volume and Issue: 88(2)

Published: March 20, 2024

SUMMARYThe genus

Language: Английский

Citations

7
Bacteria-Driven Tumor Microenvironment-Sensitive Nanoparticles Targeting Hypoxic Regions Enhances the Chemotherapy Outcome of Lung Cancer DOI Creative Commons
Huan Shi, Lan Chen,

Yanlin Liu

et al.

International Journal of Nanomedicine, Journal Year: 2023, Volume and Issue: Volume 18, P. 1299 - 1315

Published: March 1, 2023

Background: Chemotherapy still plays a dominant role in cancer treatment. However, the inability of conventional chemotherapeutic drugs to reach hypoxic zone solid tumors significantly weakens their efficacy. Bacteria-mediated drug delivery systems can be an effective targeting strategy for improving therapeutic outcomes cancer. Anaerobic bacteria have unique ability selectively transport loads regions tumors. Methods: We designed Bifidobacterium infantis (Bif)-based biohybrid ( [email protected] ) deliver polydopamine (PDA)-coated paclitaxel nanoparticles (PTX-NPs) tumor tissues. Results: The self-driven maintained toxicity PTX as well homing tendency Bif. Furthermore, inhibited growth A549 xenografts nude mice, and prolonged survival tumor-bearing mice compared other formulations without any systemic or localized toxicity. Conclusion: biohybrids provide new targeted chemotherapy Keywords: hypoxia, bifidobacterium infantis, paclitaxel, nanoparticles, lung

Language: Английский

Citations

16