Expression of Fluorescence Reporters and Natural Products in Native Gut Escherichia coli DOI
Dake Liu,

P Ton,

David M. Zong

et al.

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Escherichia coli is a widely studied model organism and an integral component of the human gut microbiome, offering significant potential for bacteria-based therapeutic applications. Despite this promise, engineering native E. strains remains challenging. In study, we employed chassis-independent recombinase-assisted genome (CRAGE) technique to genetically engineer strain EcAZ-1 probiotic Nissle 1917 (EcN). We successfully expressed suite heterologous genes, including bioluminescent lux operon, green fluorescent protein (GFP), oxygen-independent IFP2.0, in both strains. Optimization IFP2.0 fluorescence was achieved under aerobic anaerobic conditions by coexpressing heme oxygenase gene and/or supplementing chromophore biliverdin or hemin. Additionally, engineered these biosynthesize bioactive compounds naringenin mycosporine-like amino acids. This work highlights as versatile platforms synthetic biology, paving way innovative applications biomedical research development.

Language: Английский

Revolutionizing cancer treatment: The power of cell-based drug delivery systems DOI Open Access
Lu Qi, Tian Liu,

Zeyu Han

et al.

Journal of Controlled Release, Journal Year: 2023, Volume and Issue: 361, P. 604 - 620

Published: Aug. 17, 2023

Language: Английский

Citations

16
GM-CSF augmented the photothermal immunotherapeutic outcome of self-driving gold nanoparticles against a mouse CT-26 colon tumor model DOI Creative Commons
Jie Dai, Jianmei Li, Yuqin Zhang

et al.

Biomaterials Research, Journal Year: 2023, Volume and Issue: 27(1)

Published: Feb. 9, 2023

Abstract Background Hypoxia is a frequent characteristic observed in solid tumors and strongly associated with tumor metastasis, angiogenesis, drug resistance. While the vasculature of hypoxic tissues poses obstacles to efficient administration conventional drugs, it may prove advantageous sustaining hyperthermia. Photothermal therapy (PTT) offers promising treatment strategy that utilizes activation photosensitizers produce heat, thus facilitating selective ablation tissues. Method To enhance accumulation photothermal agents tissue improve effectiveness PTT, we developed self-propelled hybrid called Bif@PAu-NPs. This consists polydopamine (PDA)-coated gold nanoparticles (Au-NPs) loaded onto anaerobic Bifidobacterium infantis (Bif). Results The Bif@PAu-NPs actively aggregated at site because ability Bif can target regions, PAu-NPs achieved precise PTT due their high conversion efficiency (η = 67.8%). were ablated by resulting release antigens through immunogenic cell death (ICD), which stimulates an immune response. inclusion GM-CSF enhanced response recruiting dendritic cells initiating long-term anti-tumor immunity. Conclusion effectively suppressed growth both primary re-challenged tumors. utilization conjunction GM-SCF exhibits great potential as photothermal-immunotherapeutic for precisely treating Graphical In this study, bacterial biohybrid exploited self-driving deliver polydopamine-modified region tumor. Under irradiation 808 nm NIR laser, exerts stimulate response, further GM-CSF, leading recruitment initiation immunity remember prevent recurrence.

Language: Английский

Citations

16
The application of bacteria-nanomaterial hybrids in antitumor therapy DOI Creative Commons

Susu Xiao,

Min Mu,

Chenqian Feng

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Sept. 4, 2024

Language: Английский

Citations

6
Nanomedicine hitchhiking on bacteria for treating tumors DOI Creative Commons

Shujing Zheng,

Xingwei Li,

Shutao Guo

et al.

BMEMat, Journal Year: 2024, Volume and Issue: unknown

Published: June 25, 2024

Abstract Nanomedicine has shown promising therapeutic potential in cancer treatment, with clinically approved formulations such as Doxil® and Abraxane® already providing tangible benefits to patients. However, challenges low targeting efficiency poor tumor penetration limit its application. Bacteria have emerged drug delivery carriers due their capacity for autonomous navigation deep into hypoxic parenchyma. Therefore, utilizing bacteria nanomedicine can partially overcome the limitations of anti‐tumor nanomedicine. Moreover, some bacteria, like Salmonella typhimurium Escherichia coli , exhibit immunostimulatory oncolytic effects synergistically enhance This article summarizes common types nanomedicines respective advantages treatment. It elaborates on various strategies combining under different administration routes, outlining clinical progress bacterial therapy outlooking future applications

Language: Английский

Citations

5
Multi-functional Nanosonosensitizer-Engineered Bacteria to Overcome Tumor Hypoxia for Enhanced Sonodynamic Therapy DOI
Ting Wang, Meng Du, Zhen Yuan

et al.

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 189, P. 519 - 531

Published: Oct. 10, 2024

Language: Английский

Citations

5
Design and Evaluation of pH Sensitive PEG-Protamine Nanocomplex of Doxorubicin for Treatment of Breast Cancer DOI Open Access

Ikhlaque Ahmad,

Muhammad Farhan Ali Khan, Abbas Rahdar

et al.

Polymers, Journal Year: 2022, Volume and Issue: 14(12), P. 2403 - 2403

Published: June 14, 2022

Cancer is the most common cause of mortality worldwide. There dire need modern strategies-such as surface modification nanocarriers-to combat this global illness. Incorporation active targeting ligands has arisen a novel platform for specific tumor targeting. The aim current study was to formulate PEG-protamine complex (PPC) doxorubicin (DOX) treatment breast cancer (BC). DOX coupling with PEG can enhance cell-penetrating ability: combating resistance in MDA-MB 231 cells. Ionic gelation method adopted fabricate pH sensitive nanocomplex. optimized nanoformulation characterized its particle diameter, zeta potential, morphology, entrapment efficiency, crystallinity, and molecular interaction. In vitro assay executed gauge release potential nanoformulation. mean size, polydispersity index (PDI) nanoparticles were observed be 212 nm, 15.2 mV, 0.264, respectively. Crystallinity studies Fourier transform infrared (FTIR) analysis revealed no interaction confirmed amorphous nature drug within nanoparticles. data indicate sustained at 4.8, which intracellular cells, compared solution. PPC loaded utilized an alternative effective approach cancer.

Language: Английский

Citations

20
Stimuli-responsive nanoassemblies for targeted delivery against tumor and its microenvironment DOI

Chin Siew Sia,

Hui Peng Lim, Beng Ti Tey

et al.

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Journal Year: 2022, Volume and Issue: 1877(5), P. 188779 - 188779

Published: Aug. 14, 2022

Language: Английский

Citations

19
Establishment of ultrasound-responsive SonoBacteriaBot for targeted drug delivery and controlled release DOI Creative Commons
Meng Du, Ting Wang, Renjie Feng

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: Feb. 24, 2023

Bacteria-driven biohybrid microbots have shown great potential in cancer treatment. However, how precisely controlling drug release at the tumor site is still an issue. To overcome limitation of this system, we proposed ultrasound-responsive SonoBacteriaBot (DOX-PFP-PLGA@EcM). Doxorubicin (DOX) and perfluoro-n-pentane (PFP) were encapsulated polylactic acid-glycolic acid (PLGA) to form DOX-PFP-PLGA nanodroplets. Then, DOX-PFP-PLGA@EcM created by amide-bonded surface E. coli MG1655 (EcM). The was proved characteristics high tumor-targeting efficiency, controlled capability, ultrasound imaging. Based on acoustic phase change function nanodroplets, enhance signal US imaging after irradiation. Meanwhile, DOX loaded into can be released. After being intravenously injected, efficiently accumulate tumors without causing harm critical organs. In conclusion, has significant benefits real-time monitoring release, which applications for therapeutic delivery clinical settings.

Language: Английский

Citations

12
Bacteria-driven cancer therapy: Exploring advancements and challenges DOI Creative Commons
Syed Riaz Ud Din, Sumbul Saeed, Shahid Ullah Khan

et al.

Critical Reviews in Oncology/Hematology, Journal Year: 2023, Volume and Issue: 191, P. 104141 - 104141

Published: Sept. 23, 2023

Cancer, a serious fatal disease caused by the uncontrolled growth of cells, is biggest challenge flagging around medicine and health fields. Conventionally, various treatments-based strategies such as radiotherapy, chemotherapy, alternative cancer therapies possess drugs that cannot reach cancerous tissues make them toxic to noncancerous cells. Cancer immunotherapy has made outstanding achievements in reducing chances cancer. Our considerable attention towards cancer-directed immune responses mechanisms behind which cells kill have progressively been helpful advancement new therapies. Among them, bacteria-based achieved much more due smart robust activating host anti-tumor response. Moreover, bacterial-based therapy can be utilized single monotherapy or combination with multiple anticancer immunotherapies accelerate productive clinical results. Herein, we comprehensively reviewed recent advancements, challenges, future perspectives developing immunotherapies.

Language: Английский

Citations

12
Hacking the Immune Response to Solid Tumors: Harnessing the Anti-Cancer Capacities of Oncolytic Bacteria DOI Creative Commons

Jason M. Roe,

Kevin D. Seely, Caleb J. Bussard

et al.

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(7), P. 2004 - 2004

Published: July 21, 2023

Oncolytic bacteria are a classification of with natural ability to specifically target solid tumors and, in the process, stimulate potent immune response. Currently, these include species Klebsiella, Listeria, Mycobacteria, Streptococcus/Serratia (Coley’s Toxin), Proteus, Salmonella, and Clostridium. Advancements techniques methodology, including genetic engineering, create opportunities “hijack” typical host–pathogen interactions subsequently harness oncolytic capacities. Engineering, sometimes termed “domestication”, bacterial is especially beneficial when inaccessible or metastasize early development. This review examines reported bacteria–host details known mechanisms protein level. A synopsis presented membrane surface molecules that elicit particularly promising capacities paired stimulated localized systemic immunogenic effects. In addition, progression toward clinical translation through engineering efforts discussed, thorough attention given strains have accomplished Phase III trial initiation. addition therapeutic mitigation after tumor has formed, some species, referred as “prophylactic”, may even be able prevent “derail” formation anti-inflammatory capabilities. These their favorable characteristics summarized well. complete understanding interaction will likely necessary assess anti-cancer unlock full cancer potential bacteria.

Language: Английский

Citations

11