Intracellular and intercellular transport of RNA organelles in CXG repeat disorders: The strength of weak ties DOI Creative Commons
Deepti Kailash Nabariya, Annika Heinz,

Sabrina Derksen

et al.

Frontiers in Molecular Biosciences, Journal Year: 2022, Volume and Issue: 9

Published: Dec. 16, 2022

RNA is a vital biomolecule, the function of which tightly spatiotemporally regulated. organelles are biological structures that either membrane-less or surrounded by membrane. They produced all cells and indulge in cellular mechanisms. include intracellular granules extracellular exosomes. play an essential role regulation localization, stability translation. Aberrant connected to disease development. For example, microsatellite diseases such as CXG repeat expansion disorders, mutant RNA’s localization affected. not only transported intracellularly but can also be between via The loading exosomes regulated RNA-protein complexes, recent studies show cytosolic share common content. Intracellular exosome may therefore related. Exosomes transfer pathogenic molecules from cell cell, thereby driving progression. Both vesicles serve source for diagnostic treatment strategies. In therapeutic approaches, pharmaceutical agents loaded into then transport them desired cells/tissues. This promising target specific strategy with few side effects. With respect diagnostics, disease-specific content exosomes, e.g., RNA-signatures, attractive biomarker central nervous system detecting early physiological disturbances, even before symptoms neurodegeneration appear irreparable damage occurs. this review, we summarize known cytoplasmic vesicles, well their dysfunction disorders. We provide summary established protocols isolation characterization both organelles.

Language: Английский

Goat Milk-Derived Extracellular Vesicles Alleviate Colitis Potentially Through Improved Gut Microbiota in Mice DOI Creative Commons
Xinru Wang, Yi Liu, Chang Hong

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(9), P. 1514 - 1514

Published: April 26, 2025

Ulcerative colitis (UC) is characterized clinically by intestinal inflammation and gut microbiota dysbiosis. The consumption of biologics, although effective in control, may lead to adverse effects inconvenient for at-home administration. Goat milk-derived extracellular vesicles (GMEVs) have been proposed as a supplement prevent inflammation. However, their therapeutic potential remains elusive. This study aimed explore the preventive effect GMEVs on its underlying mechanisms through microbiota-immune axis using dextran sodium sulfate (DSS)-induced mouse model. We found that pre-treatment 20 mg/kg/d effectively prevented body weight loss, colon shortening, depletion colonic goblet cells, disappearance crypts, while enhancing mucosal barrier. Consistent with these phenotypes, GMEV increased levels IL-22 IL-10 decreased IL-1β, TNF-α, IL-6, iNOS. themselves had no normal mice. Paralleling alleviation inflammation, also restored reduction unclassified Muribaculaceae, Dubosiella, Lactobacillus suppressed expansion Alistipes Proteobacteria following DSS treatment. Additionally, intake significantly downregulated expression proteins NF-κB signaling pathway induced DSS. In summary, could regulating barrier, microbiota, organ damage, immune microenvironment. demonstrated application prospects UC prevention.

Language: Английский

Citations

0
Extracellular Vesicles from Animal Milk: Great Potentialities and Critical Issues DOI Creative Commons
Samanta Mecocci, Massimo Trabalza‐Marinucci, Katia Cappelli

et al.

Animals, Journal Year: 2022, Volume and Issue: 12(23), P. 3231 - 3231

Published: Nov. 22, 2022

Other than representing the main source of nutrition for newborn mammals, milk delivers a sophisticated signaling system from mother to child that promotes postnatal health. The bioactive components transferred through intake are important development immune and include oligosaccharides, lactoferrin, lysozyme, α-La, immunoglobulins. In last 15 years, pivotal role in this mother-to-child exchange has been attributed extracellular vesicles (EVs). EVs micro- nanosized structures enclosed phospholipidic double-layer membrane produced by all cell types released environment, reaching both close distant cells. mediate intercellular cross-talk producing receiving transfer molecules contained within them such as proteins, antigens, lipids, metabolites, RNAs, DNA fragments. complex cargo can induce wide range functional modulations recipient (i.e., anti-inflammatory, immunomodulating, angiogenetic, pro-regenerative modulations) depending on type cells stimuli these receive. be recovered every biological fluid, including blood, urine, bronchoalveolar lavage saliva, bile, milk, which is one most promising scalable vesicle sources. This review aimed present state-of-the-art animal-milk-derived EV (mEV) studies due exponential growth field. A focus beneficial potentialities human health issues studying particularly analytical methodologies applied, reported.

Language: Английский

Citations

17
Unveiling the hidden role of extracellular vesicles in brain metastases: a comprehensive review DOI Creative Commons
Ji Li,

Shuangqing Lu,

Feihu Chen

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: April 25, 2024

Extracellular vesicles (EVs) are small, transparent that can be found in various biological fluids and derived from the amplification of cell membranes. Recent studies have increasingly demonstrated EVs play a crucial regulatory role tumorigenesis development, including progression metastatic tumors distant organs. Brain metastases (BMs) highly prevalent patients with lung cancer, breast melanoma, often experience serious complications associated poor prognosis. The immune microenvironment brain was different primary tumor. Nevertheless, existing review on therapeutic potential BMs is relatively limited.

Language: Английский

Citations

3
Harnessing the power of goat milk-derived extracellular vesicles for medical breakthroughs: A review DOI
Yiming Meng,

Jing Sun,

Guirong Zhang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 262, P. 130044 - 130044

Published: Feb. 8, 2024

Language: Английский

Citations

3
Current advances in nano drug delivery system for dengue treatment and prevention DOI Creative Commons
Prajakta Jaywant Thorat, Manoj Ramesh Kumbhare, Vaibhavi Vijay Kshatriya

et al.

Intelligent Pharmacy, Journal Year: 2024, Volume and Issue: 2(5), P. 723 - 728

Published: Feb. 2, 2024

Dengue is a most important mosquito-borne viral illnesses. The disease caused by dengue viruses that have four serotypes: 1, 2, 3 and 4. Primary infection usually results in milder illness, while more severe occurs cases of repeated with different serotypes. Nanoparticles can offer significant advantages over the conventional drug delivery terms high stability, specificity, carrying capacity, ability for controlled release, possibility to use route administration capability deliver both hydrophilic hydrophobic molecules. Due prevalence viruses, it required develop novel treatment strategies provide site-specific reservoirs.

Language: Английский

Citations

2
Goat milk extracellular vesicles: Separation comparison of natural carriers for theragnostic application DOI Creative Commons

Jessie Santoro,

Silvia Nuzzo, Monica Franzese

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(6), P. e27621 - e27621

Published: March 1, 2024

Goat milk is a complex biological fluid, which in addition to having high nutritional value, it an interesting source of extracellular vesicles (EVs). Despite the countless potential applications that they offer many fields, not easy compare different proposed systems, and this major limitation for real translatability these natural nanoplatforms theragnostic purposes. Thus, useful further investigate reproducible methods separate goat EVs. The choice but also preprocessing has immense impact on separation, quality, yield Here, we tested four protocols EVs from unpasteurised milk: two based differential ultracentrifugation (DUC) size-exclusion chromatography (SEC). Moreover, assessed approaches pre-treatment (acidification precipitation) facilitate protein discharge. To best our knowledge, similar comparison all performed raw never been published before. Therefore, enriched EV samples were successfully obtained using both DUC SEC. Taken together, results may be helpful obtain carriers future personalised medicine.

Language: Английский

Citations

2
Effects of milk extracellular vesicles from bovine, human, and caprine origin on rotavirus infectivity in cultured human intestinal cells DOI

Caroline Vangsøe,

Dimitra Graikini,

Anni Bojsen

et al.

International Dairy Journal, Journal Year: 2024, Volume and Issue: 154, P. 105937 - 105937

Published: March 22, 2024

Language: Английский

Citations

2
Extracellular Vesicles in Pathogenic Infection, Transmission, and Immunity DOI Creative Commons

Junyao Xiong,

Usama Ashraf, Jing Ye

et al.

Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: July 1, 2024

Extracellular vesicles (EVs) are secreted by cells and widely exist in body fluids, serving as an essential vehicle of intercellular communication. In recent years, EVs have gained significant attention owing to their ability carry nucleic acids, lipids, proteins host pathogen origins. A distinct composition during pathogenic infection contributes mediating infection, transmission, immunity target cells. Therefore, studying the role is crucial for understanding pathogen-host interactions developing new anti-pathogenic therapies. This review offers overview current knowledge context pathogens. Harnessing EVs' biology may lay a foundation mitigation associated disease outcomes.

Language: Английский

Citations

2
Extracellular Vesicles Comprising Carborane Prepared by a Host Exchanging Reaction as a Boron Carrier for Boron Neutron Capture Therapy DOI
Riku Kawasaki,

Ayano Oshige,

Nanami Kono

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(36), P. 47137 - 47149

Published: Aug. 6, 2024

With their low immunogenicity and excellent deliverability, extracellular vesicles (EVs) are promising platforms for drug delivery systems. In this study, hydrophobic molecule loading techniques were developed via an exchange reaction based on supramolecular chemistry without using organic solvents that can induce EV disruption harmful side effects. To demonstrate the availability of exchanging to prepare drug-loading EVs, boron cluster carborane (CB) was introduced EVs (CB@EVs), which is expected as a agent neutron capture therapy (BNCT). The enabled encapsulation CB disrupting structure forming aggregates. Single-particle analysis revealed uniformly introduce cargo molecules advantageous in formulating pharmaceuticals. performance CB@EVs agents BNCT demonstrated vitro vivo. Compared L-BPA, clinically available agent, delivered with liposomes, CB@EV systems exhibited highest activity due deliverability endocytosis-independent pathway. system deeply penetrate 3D cultured spheroids even presence matrices. EV-based could efficiently accumulate tumor tissues xenograft model mice high selectivity, mainly enhanced permeation retention effect, vivo therapeutic benefits compared L-BPA/fructose complex. All features also establishing anticancer platforms.

Language: Английский

Citations

2
Polyamine-Enriched Exosomes from Leishmania donovani Drive Host Macrophage Polarization via Immunometabolism Reprogramming DOI
Prince Sebastian,

Madhulika Namdeo,

Moodu Devender

et al.

ACS Infectious Diseases, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

Leishmania donovani (Ld) promastigotes secrete exosomes that are crucial in host–pathogen interactions and intercellular communication by carrying parasite-specific molecules. Although the composition of cargos is known, effects unique metabolic repertoire on immunometabolism rewiring macrophage polarization poorly understood. Interestingly, we found enrichment polyamines (PAs) such as spermidine putrescine Ld-exosomes. Herein, investigate critical polycationic molecules their role parasite survival. Our study shows PA inhibition or depletion significantly impairs growth fitness, particularly drug-resistant strains. Furthermore, aimed to elucidate impact PAs-enriched Ld-exosomes host macrophages. The data demonstrated macrophages efficiently internalized these exosomes, leading heightened phagocytic activity infectivity. In addition, induced M2 characterized elevated Arginase-1 expression activity. increased solute carrier gene (SLC3A2) intracellular levels suggest contribute PAs pool create an anti-inflammatory milieu. These findings highlight essential survival establishing a pro-parasitic environment macrophage.

Language: Английский

Citations

2