Sympathetic chain reconstruction after failed sympathectomy for hyperhidrosis in regenerative medicine: a narrative review DOI
Francesco Petrella, Federico Raveglia, Andrea Cara

et al.

Regenerative medicine reports ., Journal Year: 2024, Volume and Issue: 1(2), P. 149 - 155

Published: Dec. 1, 2024

Hyperhidrosis is a pathologic condition characterized by abnormal sweating that greater than normally needed for proper thermoregulation. Surgical therapy remains the only effective treatment definitive cure of idiopathic focal hyperhidrosis, although some controversies still exist regarding best operation. The most significant side effect hyperhidrosis surgery compensatory hyperhidrosis. Although severe less frequent form, it results in detrimental on quality life, and restoration pre-surgical advocated affected patients. Sympathetic chain reconstruction represents surgical option managing after failed sympathetic surgery, without results. In this review, we focus experimental clinical data use compensated sympathectomy Regenerative medicine, particularly tissue engineering stem cell technology, offers new therapeutic strategies reconstruction. These may improve or resolve promoting nerve regeneration. However, there are number problems with current research, including inconsistency outcomes, lack long-term follow-up data, insufficient in-depth understanding regenerative medicine techniques. Nonetheless, progress has been made studies. For example, regeneration demonstrated animal models, different methods reconstruction, autologous grafts conduits, have shown studies provide preliminary evidence significance conclusion, development hope patients primary even though treatments not yet achieved desired With further technological technology expected to be means treating thus significantly improving life

Language: Английский

Xanthium strumarium/gelatin methacryloyl based hydrogels with anti-inflammatory and antioxidant properties for diabetic wound healing via akt/mtor pathway DOI
Zixuan Zhao, Shuyan Han,

Wei-Guo Feng

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 300, P. 140186 - 140186

Published: Jan. 27, 2025

Language: Английский

Citations

2
Advancements in lung regeneration: from bench to bedside DOI Creative Commons
Danial Khayatan, Peyman Esmaeili Fard Barzegar, Arooj Fatima

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 4, 2025

Lung disorders rank among the leading causes of mortality worldwide, presenting a critical challenge in healthcare. The underlying limited regenerative capacity lung makes this an unmet clinical need, often necessitating transplantation for patients with severe disease. However, lack viable donor organs underscores urgent need alternative therapeutic strategies. Addressing requires comprehensive understanding structure tissue and complexities inherent its regeneration. In review, we explore recent breakthroughs medicine, highlighting innovative approaches aimed at tackling tracheal diseases. From stem cell cell-based therapies to utilization biological synthetic materials, as well strategic deployment growth factors, diverse array strategies is being explored rejuvenate function. By analysis vitro, preclinical, studies, review aims provide overview emerging technology regeneration research, shedding light on promising avenues future interventions.

Language: Английский

Citations

1
Dissolving microneedle patch loaded with adipokines-enriched adipose extract relieves atopic dermatitis in mouse via modulating immune disorders, microbiota imbalance, and skin barrier defects DOI Creative Commons
Jingyan Guan, Kaiqi Chen,

Feng Lu

et al.

Journal of Tissue Engineering, Journal Year: 2025, Volume and Issue: 16

Published: Jan. 1, 2025

Atopic dermatitis (AD) is a chronic relapsing dermatosis that demands new therapies. This research group previously developed physically extracted adipose-derived extracellular matrix named adipose collagen fragments (ACF), which was determined containing massive matrix-bound adipokines and medicable on AD through intradermal injection. However, problems concerning the control of drug release inevitable pain caused by injection hinder application ACF in clinics. Microneedle (MN) rapid developing technique for precise painless transdermal delivery. Therefore, dissolving methacrylated gelatin/hyaluronic acid MN patch loaded with this study. The morphological characteristics, mechanical properties, penetration ability, as well biocompatibility degradation efficiency ACF-MN were evaluated, its efficacy ovalbumin-induced mice also investigated. exhibited excellent biocompatibility, efficiency, satisfying murine similar fresh-made ACF. Furthermore, RNA-Seq combining bioinformatics performed mechanism exploration. treatment showed comprehensive via restoring inflammatory dysregulation, microbiota imbalance, skin barrier defects. study offered novel MN-based ACF-bound delivery system may serve promising strategy relieving AD.

Language: Английский

Citations

1
Synergistic Enhancement of Therapeutic Efficacy in Acute Myocardial Infarction via Nanoflower-Like Mn3O4 Nanozymes in Coordination with Adipose-Derived Stem Cell Transplantation DOI Creative Commons
R Gao, Demeng Xia, Xiaoyong Zhang

et al.

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 2073 - 2086

Published: Feb. 1, 2025

Acute myocardial infarction (AMI) is a leading cause of mortality worldwide. Adipose-derived stem cell (ADSC) transplantation presents promising therapeutic approach for AMI; however, the harsh microenvironment infarcted myocardium, characterized by hypoxia and oxidative stress, limits survival efficacy ADSCs. Nanozymes (NZs), which have robust anti-oxidative enzyme-mimicking activities, demonstrated potential in combating stress improving viability. Mn3O4 NZs (Mn-Nzs), nanoflower-like structures were synthesized their structure multi-enzyme mimetic activities (superoxide dismutase, catalase, glutathione peroxidase) characterized. Blood biochemical parameters measured heart, liver, spleen, lungs kidneys rats, followed hematoxylin eosin (HE) staining. The impact on reactive oxygen species (ROS) levels, viability ADSCs under was assessed vitro. In vivo studies conducted using rat AMI model to evaluate ADSC transplantation, conjunction with treatment. addition, proteomic analysis performed elucidate mechanisms action underlying effects. exhibited including superoxide peroxidase, reducing levels apoptosis stress. model, Mn-NZs had good biocompatibility or treatment alone significantly reduced infarct size, fibrosis improved microvascular density heart function. Notably, combination enhanced differentiation, amplifying efficacy. Proteomic revealed that Mn3O44 upregulated proteins associated damage, anti-inflammation, anti-fibrosis pathways. MMP8 via AKT pathway phosphorylation. findings highlight novel strategy integrating NZ anti-oxidant properties improve outcomes.

Language: Английский

Citations

1
Understanding and controlling the variables for advanced cell therapy DOI Creative Commons

Naveen Jeyaraman,

Sandeep Shrivastava, Vangari Ravi

et al.

World Journal of Stem Cells, Journal Year: 2024, Volume and Issue: 16(8), P. 784 - 798

Published: Aug. 26, 2024

In regenerative medicine, the isolation of mesenchymal stromal cells (MSCs) from adipose tissue’s vascular fraction (SVF) is a critical area study. Our review meticulously examines process MSCs, starting with extraction tissue. The choice liposuction technique, anatomical site, and immediate processing are essential to maintain cell functionality. We delve into intricacies enzymatic digestion, emphasizing fine-tuning enzyme concentrations maximize yield while preventing harm. then outlines filtration centrifugation techniques necessary for isolating purified SVF, alongside viability assessments like flow cytometry, which vital confirming efficacy isolated MSCs. discuss advantages drawbacks using autologous vs allogeneic SVF sources, touching upon immunocompatibility logistical considerations, as well variability inherent in donor-derived cells. Anesthesia choices, selection between hypodermic needles cannulas, role tissue lysers achieving cellular dissociation evaluated their impact on isolation. Centrifugation protocols also analyzed part ensuring integrity SVF. necessity standardized MSC highlighted, promoting reproducibility successful clinical application. encourage ongoing research deepen understanding biology therapeutic action, aiming further field medicine. concludes call rigorous research, interdisciplinary collaboration, strict adherence ethical regulatory standards safeguard patient safety optimize treatment outcomes

Language: Английский

Citations

5
Epidermal stem cell-derived extracellular vesicles induce fibroblasts mesenchymal-epidermal transition to alleviate hypertrophic scar via the miR-200s/ZEBs axis DOI Open Access
Zhen Miao, Juntao Xie, Ruifu Yang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Abstract Hypertrophic scar (HS) is a prevalent yet unresolved wound healing complication characterized by persistent hyperactive and proliferative fibroblasts, leading to excessive extracellular matrix (ECM) synthesis collagen contraction. Our previous studies have identified epidermal stem cells (ESCs) as critical for HS remodeling, with its vesicles (EVs) playing vital role. However, the specific mechanisms remain unclear. In this study, we first discovered that ESC-EVs could effectively induce mesenchymal-epidermal transition (MET) of fibroblasts (HSFs) inhibit their biological activity. Furthermore, next-generation sequencing multiplexed CRISPR/Cas9 system, elucidated therapeutic effect mediated miR-200 family (miR-200s) encapsulated in ESC-EVs, which targeted inhibited ZEB1 ZEB2 HSFs. This role mechanism been thoroughly validated both vitro cell experiments vivo rat tail (RHS) models. These findings not only shed light on previously unidentified HS, but also provide potential novel targets strategies precise treatment.

Language: Английский

Citations

0
Microneedles in diabetic wound care: multifunctional solutions for enhanced healing DOI Creative Commons
Xichao Jian,

Y Deng,

Shune Xiao

et al.

Burns & Trauma, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 1, 2025

Abstract Diabetic wounds present a significant challenge in clinical treatment and are characterized by chronic inflammation, oxidative stress, impaired angiogenesis, peripheral neuropathy, heightened risk of infection during the healing process. By creating small channels surface skin, microneedle technology offers minimally invasive efficient approach for drug delivery treatment. This article begins outlining biological foundation normal skin wound unique pathophysiological mechanisms diabetic wounds. It then delves into various types, materials, preparation processes microneedles. The focus is on application multifunctional microneedles treatment, highlighting their antibacterial, anti-inflammatory, immunomodulatory, antioxidant, angiogenic neural repair properties. These demonstrate synergistic therapeutic effects directly influencing microenvironment, ultimately accelerating advancement not only holds promise enhancing outcomes but also new strategies addressing other

Language: Английский

Citations

0
Harnessing miR-16-5p-Loaded Exosomes from Adipose-Derived Stem Cells to Restore Immune Homeostasis in SLE patients DOI Creative Commons
Yinhong Zhang, Juan Chen,

W. Mao

et al.

Immunobiology, Journal Year: 2025, Volume and Issue: 230(3), P. 152885 - 152885

Published: March 6, 2025

Systemic Lupus Erythematosus (SLE) is an autoimmune disorder marked by imbalance between pro-inflammatory Th17 cells and regulatory T (Tregs), which contributes to chronic inflammation multi-organ damage, necessitating novel therapeutic strategies. This study investigates the potential of adipose-derived stem cell (ADSC) exosomes modulate Th17/Treg balance in SLE patients through miR-16-5p/LATS1 axis. Flow cytometry, ELISA, quantitative real-time PCR were utilized assess immune populations cytokine levels patients. Additionally, ADSC isolated characterized, their impact on CD4+ was evaluated using dual-luciferase Western blot assays. exhibited increased decreased Tregs, with corresponding changes levels. Reduced miR-16-5p expression noted cells, correlating positively Treg proportions. ADSC-derived shown deliver effectively, targeting downregulating LATS1 expression. modulation restored adjusted expression, indicating effect. exosomes, axis, offer a promising approach for restoring equilibrium. highlights exosome-based therapies modulating responses, providing foundation developing innovative treatments diseases.

Language: Английский

Citations

0
Comparative Analysis of Exosomes and Extracellular Microvesicles in Healing Pathways: Insights for Advancing Regenerative Therapies DOI Creative Commons

Mikołaj Sędzik,

Katarzyna Rakoczy, Jakub Sleziak

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(15), P. 3681 - 3681

Published: Aug. 3, 2024

Exosomes and microvesicles bear great potential to broaden therapeutic options in the clinical context. They differ genesis, size, cargo, composition despite their similarities. were identified as participating various processes such angiogenesis, cell migration, intracellular communication. Additionally, they are characterized by natural biocompatibility. Therefore, researchers concluded that could serve a novel curative method capable of achieving unprecedented results. Indeed, experiments, proved remarkably efficient enhancing wound regeneration mitigating inflammation. Despite immense advancements research on exosomes microvesicles, time for large-scale application is yet come. This article aims gather analyze current knowledge those promising particles, characteristics, implementations.

Language: Английский

Citations

3
Refining adipose-derived stem cell isolation for optimal regenerative therapy DOI Creative Commons
Chun‐Han Cheng, Wen‐Rui Hao,

Tzu-Hurng Cheng

et al.

World Journal of Stem Cells, Journal Year: 2024, Volume and Issue: 16(11), P. 978 - 984

Published: Nov. 25, 2024

This article highlights the importance of optimizing techniques used for isolating stromal vascular fraction cells from adipose tissue. Furthermore, by presenting key findings literature, it clarifies effects refined on regenerative medicine and advocates ongoing research innovation to enhance therapeutic outcomes.

Language: Английский

Citations

1