Enhancing Analysis of Extracellular Vesicles by Microfluidics

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Extracellular vesicles (EVs) play crucial roles in intercellular communication and hold great promise as biomarkers for noninvasive disease diagnosis. Intensive research efforts have been devoted to discovering the EV subpopulations responsible specific functions or with enhanced effectiveness markers, through extensive purification content analysis. However, their high heterogeneity size cargo composition poses significant challenges reaching such goals. Isolation methods like ultracentrifugation size-exclusion chromatography, well analysis approaches polymerase chain reaction enzyme-linked immunosorbent assay, made contributions improving our understanding of biology. Nonetheless, these face limitations isolation efficiency, purity, detection sensitivity specificity due issues large sample consumption, unsatisfactory insufficient resolution subtyping. Microfluidic technology presents promising solutions challenges, leveraging intrinsic capabilities precise flow external energy field manipulation, compartmentalization, signal enhancement at micro- nanoscale. Hence, this review summarizes recent developments microfluidics-enabled analysis, paying special attention unique microfluidic features exploited. Strategies viscoelastic inertial flow, fluid mixing, external-field-assisted purification, compartmentalization micro/nanostructures enhancing detection, are examined. Furthermore, current potential future directions discussed inspire advancements rapidly developing field.

Language: Английский

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Extracellular Vesicles: Hermes Between Cancers and Lymph Nodes

Cancer Letters, Journal Year: 2025, Volume and Issue: unknown, P. 217735 - 217735

Published: April 1, 2025

Language: Английский

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Neck management in cT1‐2N0 oral squamous cell carcinoma: Act or watchful wait?

International Journal of Cancer, Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Abstract The controversy over neck management for cT1‐2N0 OSCC patients has persisted two decades. While selective dissection (SND) been deemed effective, only 30% of actually exhibit lymph node metastasis (LNM). SND‐related complications, such as shoulder dysfunction and lymphedema, significantly impact patient quality life, suggesting that 70% may not benefit from SND. Current guidelines advocate observation, sentinel biopsy (SLNB), SND, but the appropriate scenarios each strategy need further exploration. Risk stratification assessment can inform treatment decisions in early‐stage OSCC. This review explores histological risk factors, SLNB, gene expression profiles, biomarkers stratification. Additionally, we assess potential value postoperative radiotherapy (PORT) immunotherapy, particularly immune checkpoint blockade (ICB), Risk‐stratified approaches align with personalized medicine precision surgery trends, while PORT ICB offer more reliable options. comprehensive systematically synthesizes past selection therapeutic strategies patients, alongside their respective strengths limitations. We aspire to contribute optimization ultimately enhancing both survival outcomes life.

Language: Английский

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Hidden messages in fluids: A review of clinical and fundamental perspectives on post‐lymph node dissection drains

International Journal of Cancer, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

Abstract In recent years, there has been a growing interest in liquid biopsy due to its non‐invasive diagnostic value. Postoperative drainage fluid (PDF) is the exudate from wound site following lymph node dissection. PDF regarded as medical waste with no specific clinical significance. Nevertheless, of may enable reuse this fluid. contains variety body fluids, including blood and lymph. biological components, cytokines, extracellular vesicles (EVs), proteins, nucleic acids, cells bacteria. These components are indicative postoperative inflammatory response, immune response therapeutic response. review, we examine current state research field PDF, elucidating how analysis can assess prognosis patients after dissection, monitor real‐time changes patient status, identify new biomarkers potential targets.

Language: Английский

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Immunometabolism in head and neck squamous cell carcinoma: Hope and challenge

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2024, Volume and Issue: unknown, P. 167629 - 167629

Published: Dec. 1, 2024

Language: Английский

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Exosomes: A potential therapeutic strategy to fight cancer as drug carriers or immune system regulators

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Journal Year: 2024, Volume and Issue: unknown, P. 189238 - 189238

Published: Dec. 1, 2024

Language: Английский

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The ending is not the end: Lymph node metastasis in oral squamous cell carcinoma

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 146, P. 113917 - 113917

Published: Dec. 24, 2024

Language: Английский

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