Reactive oxygen species from non-thermal gas plasma (CAP): implication for targeting cancer stem cells DOI Creative Commons
Amirhesam Babajani,

Afshin Eftekharinasab,

Sander Bekeschus

et al.

Cancer Cell International, Journal Year: 2024, Volume and Issue: 24(1)

Published: Oct. 22, 2024

Abstract Cancer remains a major global health challenge, with the persistence of cancer stem cells (CSCs) contributing to treatment resistance and relapse. Despite advancements in therapy, targeting CSCs presents significant hurdle. Non-thermal gas plasma, also known as CAP, represents an innovative treatment. It has recently gained attention for its often found be selective, immunogenic, potent anti-cancer properties. CAP is composed collection transient, high-energy, physically chemically active entities, such reactive oxygen species (ROS). acknowledged that latter are responsible portion biomedical effects. The dynamic interplay CAP-derived ROS other components contributes unique versatile properties enabling it interact biological systems elicit various therapeutic effects, including potential While shown promise types, application against relatively unexplored. This review assesses strategy CSCs, focusing on ability regulate cellular states achieve redox homeostasis. done by providing overview CSC characteristics demonstrating recent findings CAP’s efficacy these cells. By insights into attributes this work advanced understanding oncology strategies.

Language: Английский

Role of Silymarin in Cancer Treatment: Facts, Hypotheses, and Questions DOI Creative Commons
Tomas Koltai, Larry Fliegel

Journal of Evidence-Based Integrative Medicine, Journal Year: 2022, Volume and Issue: 27

Published: Jan. 1, 2022

The flavonoid silymarin extracted from the seeds of Sylibum marianum is a mixture 6 flavolignan isomers. 3 more important isomers are silybin (or silibinin), silydianin, and silychristin. Silybin functionally most active these compounds. This group flavonoids has been extensively studied they have used as hepato-protective substances for mushroom Amanita phalloides intoxication mainly chronic liver diseases such alcoholic cirrhosis nonalcoholic fatty liver. Hepatitis C progression not, or slightly, modified by silymarin. Recently, it also proposed SARS COVID-19 infection therapy. biochemical molecular mechanisms action in cancer subjects ongoing research. Paradoxically, many its identified actions antioxidant, promoter ribosomal synthesis, mitochondrial membrane stabilization, may seem protumoral at first sight, however, compounds clear anticancer effects. Some them are: decreasing migration through multiple targeting, hypoxia inducible factor-1α expression, inducing apoptosis some malignant cells, inhibiting promitotic signaling among others. Interestingly, antitumoral activity limited to cells while nonmalignant not be affected. Furthermore, there long history use human without toxicity after prolonged administration. ample distribution easy accessibility milk thistle—the source compounds, over counter availability, fact that weed, controversial issues regarding bioavailability, being nutraceutical rather than drug, somehow led medical professionals view effects with skepticism. fundamental reason why never achieved bedside status treatment. However, spite all effects, actually dual cases pancreatic can promote stemness. review deals recent investigations elucidate this cancer, consider possibility repurposing it. Particular attention dedicated silymarin's role controversies real effectiveness.

Language: Английский

Citations

73
Medical gas plasma technology: Roadmap on cancer treatment and immunotherapy DOI Creative Commons
Sander Bekeschus

Redox Biology, Journal Year: 2023, Volume and Issue: 65, P. 102798 - 102798

Published: June 27, 2023

Despite continuous therapeutic progress, cancer remains an often fatal disease. In the early 2010s, first evidence in rodent models suggested promising antitumor action of gas plasma technology. Medical is a partially ionized depositing multiple physico-chemical effectors onto tissues, especially reactive oxygen and nitrogen species (ROS/RNS). Today, evergrowing body experimental suggests multifaceted roles medical plasma-derived ROS/RNS targeting alone or combination with oncological treatment schemes such as ionizing radiation, chemotherapy, immunotherapy. Intriguingly, technology was recently unraveled to have immunological dimension by inducing immunogenic cell death, which could ultimately promote existing immunotherapies via situ autologous tumor vaccine schemes. Together clinical reporting beneficial effects patients following therapy, it time summarize main concepts along chances limitations onco-therapy from biological, immunological, clinical, technological point view.

Language: Английский

Citations

45
Applications and Enhancement Strategies of ROS-based Non-invasive Therapies in Cancer Treatment DOI Creative Commons
Qiuyan Guo,

Yingnan Tang,

Shengmei Wang

et al.

Redox Biology, Journal Year: 2025, Volume and Issue: unknown, P. 103515 - 103515

Published: Jan. 1, 2025

Language: Английский

Citations

2
Medical Gas Plasma Jet Technology Targets Murine Melanoma in an Immunogenic Fashion DOI Creative Commons
Sander Bekeschus, Ramona Clemen,

Felix Nießner

et al.

Advanced Science, Journal Year: 2020, Volume and Issue: 7(10)

Published: March 30, 2020

Medical technologies from physics are imperative in the diagnosis and therapy of many types diseases. In 2013, a novel cold physical plasma treatment concept was accredited for clinical therapy. This gas jet technology generates large amounts different reactive oxygen nitrogen species (ROS). Using melanoma model, is tested as anticancer agent. Plasma derived ROS diminish tumor growth vitro vivo. Varying feed mixture modifies composition ROS. Conditions rich atomic correlate with killing activity elevate intratumoral immune-infiltrates CD8

Language: Английский

Citations

105
Cold atmospheric plasma induces apoptosis in human colon and lung cancer cells through modulating mitochondrial pathway DOI Creative Commons
Yanhong Wang,

Xinyu Mang,

Xuran Li

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 10

Published: July 26, 2022

Cold atmospheric plasma (CAP) is an emerging and promising oncotherapy with considerable potential advantages that traditional treatment modalities lack. The objective of this study was to investigate the effect mechanism plasma-inhibited proliferation plasma-induced apoptosis on human lung cancer colon cells in vitro vivo . Piezobrush ® PZ2, a handheld CAP unit based piezoelectric direct discharge technology, used generate deliver non-thermal plasma. Firstly, PZ2 inhibited HT29 colorectal A549 using CCK8 assay, caused morphological changes at cellular subcellular levels transmission electron microscopy, suppressed both types tumor cell migration invasion Transwell Matrigel assay. Secondly, we confirmed AO/EB staining coupled flow cytometry, verified production apoptosis-related proteins, such as cytochrome c, PARP, cleaved caspase-3 caspase-9, Bcl-2 Bax, western blotting. Finally, aforementioned results were tested cell-derived xenograft mouse models, anticancer attributed CAP-mediated apoptosis. immunohistochemical analysis revealed expression caspase-3, PARP Bax upregulated whereas downregulated after treatment. These findings collectively suggest activation mitochondrial pathway involved during -induced

Language: Английский

Citations

41
Critical review of therapeutic potential of silymarin in cancer: A bioactive polyphenolic flavonoid DOI Creative Commons
Mehdi Koushki, Reyhaneh Farrokhi Yekta, Nasrin Amiri‐Dashatan

et al.

Journal of Functional Foods, Journal Year: 2023, Volume and Issue: 104, P. 105502 - 105502

Published: March 21, 2023

Cancer is identified as second leading causes of death and imposes a significant psychological economic burden on societies. Due to its high prevalence, main cause worldwide, there continuing need discover new potential anticancer compounds. Plant-derived compounds have recently attracted more attention among researchers for their potent anti-cancer properties. A numerous research, including in-vitro, in-vivo, preclinical, clinical, epidemiological studies, has indicated that the flavonoid silymarin comprehensive preventive therapeutic effects array inflammation-driven diseases, cancer. Silymarin clear activities through distinctive mechanisms modulation apoptosis in-vitro survival in-vivo. The chemoprotection constituent suggest they could be promising agents in various cancer types. This review focus vivo vitro variety cancers, explores signaling pathways modulated by this compound.

Language: Английский

Citations

28
Cold atmospheric plasma applications in dermatology: A systematic review DOI
Lu Gan, Jiang Jian,

Jiang Wei Duan

et al.

Journal of Biophotonics, Journal Year: 2020, Volume and Issue: 14(3)

Published: Nov. 24, 2020

Abstract Cold atmospheric plasma (CAP) applications can potentially lead to effective therapy for numerous skin diseases. Our aim is systematically review the available data and map use of CAP in dermatology. PubMed, Embase Web science were explored before 2020 studies regarding A total 166 finally included. 74.1% these used indirect sources. Most jet (67.5%). Argon was mostly working gas (48.2%). Plasma application itself could be direct (89.2%) (16.3%). The proportion with vivo results remained 57.2%, which most concerned treatment (97.9%). Analyses performed indicate that has been beneficial many disorders. While, focused on wound healing melanoma treatment. This study provides a brief overview sources relative medical

Language: Английский

Citations

55
Cold Physical Plasma in Cancer Therapy: Mechanisms, Signaling, and Immunity DOI Creative Commons
Fatemeh Faramarzi, Parisa Zafari, Mina Alimohammadi

et al.

Oxidative Medicine and Cellular Longevity, Journal Year: 2021, Volume and Issue: 2021(1)

Published: Jan. 1, 2021

Despite recent advances in therapy, cancer still is a devastating and life‐threatening disease, motivating novel research lines oncology. Cold physical plasma, partially ionized gas, new modality research. Physical plasma produces various physicochemical factors, primarily reactive oxygen nitrogen species (ROS/RNS), causing cell death when supplied at supraphysiological concentrations. This review outlines the biomedical consequences of treatment experimental including modalities. It also summarizes current knowledge on intracellular signaling pathways triggered by to induce death. Besides inactivation tumor cells, an equally important aspect inflammatory context which occurs suppress or promote responses immune cells. mainly governed release damage‐associated molecular patterns (DAMPs) provoke immunogenic (ICD) that, turn, activates cells innate system adaptive antitumor immunity. The pivotal role treatment, general, highlighted many clinical trials success stories using checkpoint immunotherapy. Hence, potential ICD immunity targeting lesions systemically discussed.

Language: Английский

Citations

54
Tumor cell metabolism correlates with resistance to gas plasma treatment: The evaluation of three dogmas DOI
Sander Bekeschus,

Grit Liebelt,

Jonas Menz

et al.

Free Radical Biology and Medicine, Journal Year: 2021, Volume and Issue: 167, P. 12 - 28

Published: March 9, 2021

Language: Английский

Citations

48
Epithelial-to-Mesenchymal Transition Enhances Cancer Cell Sensitivity to Cytotoxic Effects of Cold Atmospheric Plasmas in Breast and Bladder Cancer Systems DOI Open Access
Peiyu Wang, Renwu Zhou, Patrick B. Thomas

et al.

Cancers, Journal Year: 2021, Volume and Issue: 13(12), P. 2889 - 2889

Published: June 9, 2021

Cold atmospheric plasma (CAP) has emerged as a highly selective anticancer agent, most recently in the form of plasma-activated medium (PAM). Since epithelial-mesenchymal transition (EMT) been implicated resistance to various cancer therapies, we assessed whether EMT status is associated with PAM response. Mesenchymal breast cell lines, well mesenchymal variant an isogenic EMT/MET human system (PMC42-ET/LA), were more sensitive treatment than their epithelial counterparts, contrary responses other therapies. The same trend was seen luminal muscle-invasive bladder model (TSU-Pr1/B1/B2) and non-muscle-invasive basal 5637 line. Three-dimensional spheroid cultures lines less compared two-dimensional counterparts; however, incrementally better again mesenchymally-shifted lines. This study provides evidence that preferentially inhibits carcinoma cells, which have Thus, may represent novel can selectively inhibit triple-negative cancers subset aggressive cancers, tend be mesenchymal. Our approach potentially utilized for exhibiting opens new opportunities CAP promising onco-therapy.

Language: Английский

Citations

48