Role of Leptin and Adiponectin in Carcinogenesis

Cancers, Journal Year: 2023, Volume and Issue: 15(17), P. 4250 - 4250

Published: Aug. 24, 2023

Hormones produced by adipocytes, leptin and adiponectin, are associated with the process of carcinogenesis. Both these adipokines have well-proven oncologic potential can affect many aspects tumorigenesis, from initiation primary tumor growth to metastatic progression. Involvement in formation cancer includes interactions microenvironment its components, such as tumor-associated macrophages, cancer-associated fibroblasts, extracellular matrix metalloproteinases. Furthermore, participate epithelial–mesenchymal transition connect angiogenesis, which is critical for invasiveness cell migration. In addition, an enormous amount evidence has demonstrated that altered concentrations adipocyte-derived hormones expression their receptors tumors poor prognosis various types cancer. Therefore, adiponectin dysfunction play a prominent role impact invasion metastasis different ways. This review clearly comprehensively summarizes recent findings presents initiation, promotion progression, focusing on associations components well roles angiogenesis.

Language: Английский

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KLF2 inhibits colorectal cancer progression and metastasis by inducing ferroptosis via the PI3K/AKT signaling pathway

The Journal of Pathology Clinical Research, Journal Year: 2023, Volume and Issue: 9(5), P. 423 - 435

Published: May 6, 2023

Abstract Krüppel‐like factor 2 (KLF2) belongs to the zinc finger family and is thought be a tumor suppressor gene due its low expression in various cancer types. However, functional role molecular pathway involvement colorectal (CRC) are not well defined. Herein, we investigated potential mechanism of KLF2 CRC cell invasion, migration, epithelial–mesenchymal transition (EMT). We utilized TCGA GEPIA databases analyze patients correlation with different stages prognosis. RT‐PCR, western blot, immunohistochemistry assays were used measure expression. Gain‐of‐function performed evaluate progression. Moreover, mechanistic experiments conducted investigate involved signaling pathways regulated by KLF2. Additionally, also xenograft assay tumorigenesis. was patient tissues lines, associated poor Remarkably, overexpressing significantly inhibited EMT capabilities cells, growth xenografts. Mechanistically, overexpression induced ferroptosis cells regulating glutathione peroxidase 4 this KLF2‐dependent mediated inhibiting PI3K/AKT that resulted suppression cells. report for first time acts as inducing via pathway, thus providing new direction prognosis assessment targeted therapy.

Language: Английский

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Electroactive 4D Porous Scaffold Based on Conducting Polymer as a Responsive and Dynamic In Vitro Cell Culture Platform

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(5), P. 5613 - 5626

Published: Jan. 26, 2024

, cells reside in a 3D porous and dynamic microenvironment. It provides biochemical biophysical cues that regulate cell behavior physiological pathological processes. In the context of fundamental biology research, tissue engineering, cell-based drug screening systems, challenge is to develop relevant

Language: Английский

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Unraveling the roles of miRNAs in regulating epithelial-to-mesenchymal transition (EMT) in osteosarcoma

Pharmacological Research, Journal Year: 2021, Volume and Issue: 172, P. 105818 - 105818

Published: Aug. 13, 2021

Language: Английский

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Viscoelasticity, Like Forces, Plays a Role in Mechanotransduction

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 10

Published: Feb. 9, 2022

Viscoelasticity and its alteration in time space has turned out to act as a key element fundamental biological processes living systems, such morphogenesis motility. Based on experimental theoretical findings it can be proposed that viscoelasticity of cells, spheroids tissues seems collective characteristic demands macromolecular, intracellular component intercellular interactions. A major challenge is couple the alterations macroscopic structural or material characteristics tissues, cell tissue phase transitions, microscopic interferences their elements. Therefore, biophysical technologies need improved, advanced connected classical assays. In this review, viscoelastic nature cytoskeletal, extracellular cellular networks presented discussed. conceptualized contributor migration invasion discussed whether serve biomarker for cells' migratory capacity several contexts. It hypothesized statistical mechanics intra- may applied future powerful tool explore quantitatively biomechanical foundation over broad range length scales. Finally, importance illustrated identifying characterizing multiple disorders, cancer, injuries, acute chronic inflammations fibrotic diseases.

Language: Английский

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Prostate cancer cells of increasing metastatic potential exhibit diverse contractile forces, cell stiffness, and motility in a microenvironment stiffness-dependent manner

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 10

Published: Sept. 19, 2022

During metastasis, all cancer types must migrate through crowded multicellular environments. Simultaneously, cancers appear to change their biophysical properties. Indeed, cell softening and increased contractility are emerging as seemingly ubiquitous biomarkers of metastatic progression which may facilitate metastasis. Cell stiffness also influenced by the microenvironment. Stiffer matrices resembling tumor microenvironment cause cells contract more strongly, further promoting contractile tumorigenic phenotypes. Prostate (PCa), however, appears deviate from these common biophysics trends; aggressive PCa stiffer, rather than softer, lowly counterparts. Although have been reported be healthy cells, how changes with increasing potential has remained unknown. Here, we characterize various a function stiffness. Using panel progressively lines (22RV1, LNCaP, DU145, PC3), quantified using traction force microscopy (TFM), measured cortical optical magnetic twisting cytometry (OMTC) motility time-lapse microscopy. We found that contractility, stiffness, do not universally scale potential. Rather, efficiencies exhibit unique responses differentially substrate Despite this diversity, work concludes mechanical is key determinant in response variable potentials. The mechanics-oriented focus methodology study complementary conventional biochemical genetic strategies typically used understand disease, thus usher new perspectives approaches.

Language: Английский

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Acute myeloid leukemia (AML)‐derived mesenchymal stem cells induce chemoresistance and epithelial–mesenchymal transition‐like program in AML through IL‐6/JAK2/STAT3 signaling

Cancer Science, Journal Year: 2023, Volume and Issue: 114(8), P. 3287 - 3300

Published: June 4, 2023

Abstract Acute myeloid leukemia (AML) has a high rate of treatment failure due to increased prevalence therapy resistance. Mesenchymal stem cells (MSCs) in the microenvironment contribute chemoresistance AML, but specific mechanism remains unclear. The critical role epithelial–mesenchymal transition (EMT)‐like profile AML been gradually recognized. However, there is no research suggest that AML‐derived bone marrow mesenchymal (AML‐MSCs) induce EMT program thus far. We isolated AML‐MSCs and cocultured them with cells. found induced significant mesenchymal‐like morphology drug‐resistant cells, it was scarce parental promoted growth presence or absence chemotherapeutics vitro vivo. MSCs also marker expression especially chemoresistant Mechanistically, secreted abundant interleukin‐6 (IL‐6) upregulated IL‐6 turn. Meanwhile, activated JAK2/STAT3 pathway Two JAK/STAT inhibitors counteracted change In conclusion, not only promote emergence enhance once acquires chemoresistance. EMT‐like features cells; this phenotypic could be related progression. through IL‐6/JAK2/STAT3 signaling, which provides therapeutic target reverse AML.

Language: Английский

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Unraveling the molecular mechanisms of cell migration impairment and apoptosis associated with steroid sulfatase deficiency: Implications for X-linked ichthyosis

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2024, Volume and Issue: 1870(3), P. 167004 - 167004

Published: Jan. 3, 2024

Language: Английский

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3D Traction Force Microscopy in Biological Gels: From Single Cells to Multicellular Spheroids

Annual Review of Biomedical Engineering, Journal Year: 2024, Volume and Issue: 26(1), P. 93 - 118

Published: Feb. 5, 2024

Cell traction force plays a critical role in directing cellular functions, such as proliferation, migration, and differentiation. Current understanding of cell is largely derived from 2D measurements where cells are plated on substrates. However, do not recapitulate vital aspect living systems; that is, actively remodel their surrounding extracellular matrix (ECM), the remodeled ECM, return, can have profound impact phenotype generation. This reciprocal adaptivity systems encoded material properties biological gels. In this review, we summarize recent progress measuring for embedded within 3D gels, with an emphasis cell-ECM cross talk. We also provide perspectives tools techniques could be adapted to measure complex biochemical biophysical environments.

Language: Английский

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Exploring the Immunological Profile in Breast Cancer: Recent Advances in Diagnosis and Prognosis through Circulating Tumor Cells

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(9), P. 4832 - 4832

Published: April 29, 2024

This review offers a comprehensive exploration of the intricate immunological landscape breast cancer (BC), focusing on recent advances in diagnosis and prognosis through analysis circulating tumor cells (CTCs). Positioned within broader context BC research, it underscores pivotal role immune system shaping disease’s progression. The primary objective this investigation is to synthesize current knowledge aspects BC, with particular emphasis diagnostic prognostic potential offered by CTCs. adopts thorough examination relevant literature, incorporating breakthroughs field. methodology section succinctly outlines approach, specific focus CTC its implications for prognosis. Through review, insights into dynamic interplay between are highlighted, CTCs advancing methodologies refining assessments. Furthermore, presents substantiated results, contributing deeper understanding complexity BC. In conclusion, significance exploring profile patients, providing valuable novel utilization presentation findings emphasizes crucial dynamics, thereby opening avenues enhanced clinical management strategies.

Language: Английский

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