Bacterial Biofilm Formation on Biomaterials and Approaches to Its Treatment and Prevention

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(14), P. 11680 - 11680

Published: July 20, 2023

Bacterial biofilms can cause widespread infection. In addition to causing urinary tract infections and pulmonary in patients with cystic fibrosis, help microorganisms adhere the surfaces of various medical devices, biofilm-associated on biomaterials such as venous ducts, joint prostheses, mechanical heart valves, catheters. Biofilms provide a protective barrier for bacteria resistance antimicrobial agents, which increases morbidity mortality patients. This review summarizes biofilm formation processes mechanisms, well main features clinically persistent caused by biofilms. Considering clinical we introduce two methods prevent treat biomaterial-related infection: antibacterial coatings surface modification biomaterials. Antibacterial depend covalent immobilization agents coating drug release combat infection, while affects adhesion behavior cells implants subsequent process altering physical chemical properties implant material surface. The advantages each strategy terms their effect, biocompatibility, limitations, application prospects are analyzed, providing ideas research directions development novel infection strategies related therapeutic materials.

Language: Английский

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History of periodontitis as a risk factor for implant failure and incidence of peri‐implantitis: A systematic review, meta‐analysis, and trial sequential analysis of prospective cohort studies

Clinical Implant Dentistry and Related Research, Journal Year: 2024, Volume and Issue: 26(3), P. 482 - 508

Published: May 8, 2024

Abstract Introduction Dental implants are widely employed as dependable replacements for lost teeth. However, it is crucial to establish, solely through prospective cohort studies, whether a history of periodontitis indeed constitutes significant risk factor implant failure. Methods A systematic literature search was conducted in October 2022 several electronic databases with subsequent manual updates. Only original studies evaluating the (loss) rate ≥1 year after loading were included. Logarithmic ratio and weighted mean differences calculated. Study results summarized using random effects meta‐analyses evaluated by trial sequential analyses. The Newcastle‐Ottawa scale study bias GRADE approach assessed certainty/quality evidence. Results total 14 publications reporting on 12 Low evidence due absence randomized clinical trials revealed significantly greater odds failure patients at follow‐ups both ≤5 years (RR = 1.62; 95% CI: 1.71–2.37; p 0.013) >5 2.26; 1.12–4.53; 0.023). incidence peri‐implantitis 4.09; 1.93–8.58; < 0.001) (WM) marginal bone loss (WM difference 0.75 mm; 0.18–1.31; 0.05) statistically periodontally compromised group, whereas there no between two groups peri‐implant probing depth. Conclusion can be considered incident failure, peri‐implantitis, loss.

Language: Английский

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Microbial dysbiosis in periodontitis and peri-implantitis: pathogenesis, immune responses, and therapeutic

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Feb. 11, 2025

The oral microbiome comprises over 700 distinct species, forming complex biofilms essential for maintaining and systemic health. When the microbial homeostasis in periodontium is disrupted, pathogens within biofilm can cause periodontitis peri-implantitis, inducing host immune responses. Understanding role of communities mechanisms health disease crucial developing improved preventive, diagnostic therapeutic strategies. However, many questions remain about how changes bacterial populations contribute to development progression these conditions. An electronic manual literature search was conducted using PubMed, Excerpta Medica, Frontiers Reports Wiley Online Library databases relevant articles. Data from publications were extracted overall findings summarized a narrative manner. variations responses peri-implantitis are explored. Dysbiosis subgingival microbiome—characterized by an increase pathogenic bacteria such as Porphyromonas gingivalis , Tannerella forsythia Aggregatibacter actinomycetemcomitans —plays pivotal initiation periodontitis. As alterations include higher abundance opportunistic reduced diversity around implants. Moreover, dysbiosis potentially influencing through immune-mediated pathways. Regional immunity involving neutrophils, T helper cells-17, immune-related cytokines periodontal responding imbalances. Additionally, impact non-mechanical treatments—such probiotics laser therapy—on discussed, demonstrating their potential managing dysbiosis. These underscore that central factor peri-implantitis. Maintaining balance preventing diseases, interventions targeting could enhance treatment outcomes. Strategies focusing on controlling bacteria, modulating responses, promoting tissue regeneration key restoring stability. Further research needed clarify underlying transition peri-implant mucositis optimize prevention approaches, considering interactions between immunity.

Language: Английский

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Osteogenic Microenvironment Restoration around Dental Implants Induced by an Injectable MXene-Based Hydrogel

ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: 7(11), P. 13071 - 13088

Published: May 23, 2024

Peri-implantitis is an excessive inflammatory response induced by complex interactions between the bacteria, host immune system, and tissues around dental implant. Alveolar bone resorption caused peri-implantitis a significant cause of implant failure. However, to restore osteogenic microenvironment, multifaceted therapeutic method that simultaneously encompasses anti-inflammatory tissue regenerative strategies indispensable. In this study, temperature-sensitive hydrogel (FMXO) loaded with MXene MgO was used develop multifunctional injectable MXene-based hydrogel. This could release in irregular defect areas situ no toxicity, either vitro or vivo. Our results showed FMXO effectively inhibited NF-κB signaling pathway RAW264.7 cells upregulated expression ALP, Runx2, OCN marrow mesenchymal stromal (BMSCs). Therefore, exerted pharmacological effects on inflammation regeneration. Moreover, we found FMXO, when exposed near-infrared (NIR) light, exhibited high efficacy against Porphyromonas gingivalis, Fusobacterium nucleatum, their plaque microorganisms, which were considered initiating factors peri-implantitis. The multifunctionality also confirmed immediate peri-implant rat model vivo, suggesting great potential for therapy clinic.

Language: Английский

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Peri-Implantitis-Associated Microbiota before and after Peri-Implantitis Treatment, the Biofilm “Competitive Balancing” Effect: A Systematic Review of Randomized Controlled Trials

Microorganisms, Journal Year: 2024, Volume and Issue: 12(10), P. 1965 - 1965

Published: Sept. 28, 2024

This systematic review of RCTs aimed to characterize short- and long-term changes in peri-implantitis-associated microbiota (total biofilm microbial load predominant pathogens' counts) following (any) peri-implantitis treatment systemically healthy, non-smoking, partially/totally edentulous adults. The study protocol, compliant with the PRISMA statement, was registered on PROSPERO (CRD42024514521) before literature search. Data from 11 RCTs, assessed through ROBINS-2 tool, were qualitatively synthesized. No data retrieved total edentulism, healthy peri-implant/periodontal sites, treated mucositis, gingivitis, periodontitis sites. Shortly after treatment,

Language: Английский

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The progress in titanium alloys used as biomedical implants: From the view of reactive oxygen species

Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10

Published: Dec. 19, 2022

Titanium and alloys are widely used as biomedical implants in oral maxillofacial surgery, due to superior mechanical properties biocompatibility. In specific clinical populations such the elderly, diabetics patients with metabolic diseases, failure rate of medical metal is increased significantly, putting them at risk revision surgery. Many studies show that content reactive oxygen species (ROS) microenvironment bone tissue surrounding implant materials undergoing addition, size shape materials, morphology, wettability, properties, other play significant roles production ROS. The accumulated ROS break original balance oxidation anti-oxidation, resulting host oxidative stress. It may accelerate degradation mainly by activating inflammatory cells. Peri-implantitis usually leads a loss mass around implant, which tends affect long-term stability longevity implant. Therefore, great deal research urgently needed focus on developing antibacterial technologies. addition active elements titanium greatly reduce postoperative infection patients. Besides, innovative technologies new biomaterials surfaces conferring anti-infective rely can be considered act messenger substance for communication between implanted material, run through entire wound repair process role cannot ignored. necessary understand interaction stress effects products osseointegration life well ROS-induced bactericidal activity. This helps facilitate development generation well-biocompatible responsiveness, ultimately prolong lifespan implants.

Language: Английский

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Graphene oxide/ε-poly-L-lysine self-assembled functionalized coatings improve the biocompatibility and antibacterial properties of titanium implants

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: April 4, 2024

The construction of an antibacterial biological coating on titanium surface plays important role in the long-term stability oral implant restoration. Graphene oxide (GO) has been widely studied because its excellent properties and osteogenic activity. However, striking a balance between toxicity remains significant challenge with GO. ε-poly-L-lysine (PLL) broad-spectrum activity ultra-high safety performance. Using Layer-by-layer self-assembly technology (LBL), different layers PLL/GO coatings GO were assembled sheet. materials characterized using scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron (XPS) contact angle test. Porphyromonas gingivalis ( P.g .) analyzed through SEM, coated plate experiment, inhibition zone experiment. CCK-8 was used to determine cytotoxicity material MC3T3 cells, zebrafish larvae embryos developmental inflammatory effects material. results show that combined assembly 20 PLL exhibits good no toxicity, suggesting potential application for titanium-based modification scheme.

Language: Английский

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Guided Plasma Application in Dentistry—An Alternative to Antibiotic Therapy

Antibiotics, Journal Year: 2024, Volume and Issue: 13(8), P. 735 - 735

Published: Aug. 5, 2024

Cold atmospheric plasma (CAP) is a promising alternative to antibiotics and chemical substances in dentistry that can reduce the risk of unwanted side effects bacterial resistance. AmbiJet device ignite deliver directly site action for maximum effectiveness. The aim study was investigate its antimicrobial efficacy possible development effect tested under aerobic anaerobic conditions on bacteria (five aerobic, three (Gram +/−)) are relevant dentistry. application times varied from 1 7 min. Possible resistance evaluated by repeated applications (10 50 days). A increase temperature measured. Plasma effectively killed 106 seeded after an time min per 10 mm2. Neither nor above 40 °C observed, so patient discomfort be ruled out. treatment proved effective conditions, influence ROS questioned. Our results show efficiently eliminates pathogenic oral bacteria. Therefore, it advocated clinical therapeutic use.

Language: Английский

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The Oral Microbiome of Peri-Implant Health and Disease: A Narrative Review

Dentistry Journal, Journal Year: 2024, Volume and Issue: 12(10), P. 299 - 299

Published: Sept. 24, 2024

Peri-implantitis disease has increased significantly over the last years, resulting in failure of implants. Many factors may play a role implant complications and failure, including ones related to oral microbiota. This literature review aims summarize current knowledge microbiome implants health disease, focusing not only on presence/absence specific microbiota or their relative abundance, but also phenotypic expression complex relationships with host. The authors examined MEDLINE database identified key topics about peri-implant disease. differs from that tooth, both as they are structurally chemically different. adhesion formation biofilm can be affected by surface energy, topography, wettability, electrochemical charges surface. In addition, morphogenesis tissues surrounding dental making more susceptible bacterial infection. interplay between host immune system infections still needs elucidated.

Language: Английский

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Single-cell RNA sequencing reveals the role of immune-related autophagy in aseptic loosening of biomaterials bone-implant

Biomaterials Advances, Journal Year: 2025, Volume and Issue: 169, P. 214190 - 214190

Published: Jan. 16, 2025

Language: Английский

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