Biotechnology and Genetic Engineering Reviews,
Journal Year:
2023,
Volume and Issue:
40(4), P. 4361 - 4379
Published: May 8, 2023
We
investigate
the
impact
of
bovine
pulmonary
surfactant
(PS)
on
LPS-induced
ALI
in
vitro
and
vivo
to
improve
recognition
prevent
mortality
sepsis-induced
ALI.
Primary
alveolar
type
II
(AT2)
cells
were
treated
with
LPS
alone
or
combination
PS.
Cell
morphology
observation,
CCK-8
proliferation
assay,
flow
cytometry
apoptosis
ELISA
for
inflammatory
cytokine
levels
performed
at
different
time
points
after
treatment.
An
rat
model
was
established
vehicle
Lung
wet/dry
weight
ratio,
histopathological
changes,
lung
function
parameters,
serum
examined
6
h
PS
Survival
analysis
by
Kaplan-Meier
method.
RNA
sequencing
conducted
identify
differentially
expressed
genes
lungs.
Proapoptotic
gene
expression
lungs
determined
Western
blot.
significantly
inhibited
cell
while
promoting
AT2
starting
2
treatment,
accompanied
a
significant
increase
production;
reversed
these
effects.
decreased
ratio
septic
rats,
histological
abnormalities,
alterations
cytokines
improving
overall
survival
rats.
closely
associated
apoptosis.
attenuated
upregulation
proapoptotic
treatment
restoring
ATPase
activity
vivo.
Bovine
alleviates
early
phase,
possibly
suppressing
inflammation
apoptosis,
as
preemptive
therapeutic
agent
managing
Biomedicine & Pharmacotherapy,
Journal Year:
2024,
Volume and Issue:
176, P. 116802 - 116802
Published: May 24, 2024
Oxidative
stress
plays
a
key
role
in
chronic
kidney
disease
(CKD)
development
and
progression,
inducing
cell
damage,
inflammation,
fibrosis.
However,
effective
therapeutic
interventions
to
slow
down
CKD
advancement
are
currently
lacking.
The
multifaceted
pharmacological
effects
of
molecular
hydrogen
(H2)
have
made
it
promising
avenue.
H2
is
capable
capturing
harmful
•OH
ONOO-
while
maintaining
the
crucial
reactive
oxygen
species
(ROS)
involved
cellular
signaling.
NRF2-KEAP1
system,
which
manages
redox
balance,
could
be
used
treat
CKD.
activates
this
pathway,
fortifying
antioxidant
defenses
scavenging
ROS
counteract
oxidative
stress.
can
improve
NRF2
signaling
by
using
Wnt/β-catenin
pathway
indirectly
activate
mitochondria.
Additionally,
modulates
NF-κB
activity
regulating
status,
inhibiting
MAPK
pathways,
Trx
levels.
Treatment
with
also
attenuates
HIF
neutralizing
bolstering
HIF-1α
function.
Furthermore,
affects
FOXO
factors
enhances
enzymes.
Despite
encouraging
results
bench
studies,
clinical
trials
still
limited
require
further
investigation.
focus
review
on
hydrogen's
treating
renal
diseases,
specific
regulation,
discusses
its
potential
applications.
Frontiers in Cell and Developmental Biology,
Journal Year:
2023,
Volume and Issue:
11
Published: Nov. 7, 2023
As
a
novel
antioxidant,
growing
body
of
studies
has
documented
the
diverse
biological
effects
molecular
hydrogen
(H
2
)
in
wide
range
organisms,
spanning
animals,
plants,
and
microorganisms.
Although
several
possible
mechanisms
have
been
proposed,
they
cannot
fully
explain
extensive
H
.
Mitochondria,
known
for
ATP
production,
also
play
crucial
roles
cellular
functions,
including
Ca
2+
signaling,
regulation
reactive
oxygen
species
(ROS)
generation,
apoptosis,
proliferation,
lipid
transport,
while
their
dysfunction
is
implicated
broad
spectrum
diseases,
cardiovascular
disorders,
neurodegenerative
conditions,
metabolic
cancer.
This
review
aims
to
1)
summarize
experimental
evidence
on
impact
mitochondrial
function;
2)
provide
an
overview
pathways
underlying
,
3)
discuss
metabolism
eukaryotic
organisms
its
relationship
with
mitochondria.
Moreover,
based
previous
findings,
this
proposes
that
may
regulate
quality
control
through
response
varying
degrees
damage.
By
combining
existing
research
evolutionary
perspective,
emphasizes
potential
hydrogenase
activity
mitochondria
higher
plants
animals.
Finally,
addresses
issues
current
mechanistic
study
offers
insights
into
future
directions,
aiming
reference
action
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(15), P. 12477 - 12477
Published: Aug. 5, 2023
Molecular
hydrogen
(H2)
has
been
recognized
as
a
novel
medical
gas
with
antioxidant
and
anti-inflammatory
effects.
Non-alcoholic
fatty
liver
disease
(NAFLD)
is
pathology
increased
fat
accumulation
in
tissue
caused
by
factors
other
than
alcohol
consumption.
Platelet
mitochondrial
function
considered
to
reflect
systemic
health.
We
studied
the
effect
of
adjuvant
therapy
hydrogen-rich
water
(HRW)
on
coenzyme
Q10
(CoQ10)
content
platelet
bioenergetics
patients
NAFLD.
A
total
30
NAFLD
15
healthy
volunteers
were
included
this
clinical
trial.
17
(H2
group)
drank
three
×
330
mL/day
tablets
producing
HRW
(>4
mg/L
H2)
for
8
weeks,
13
(P
placebo
CO2.
The
concentration
CoQ10-TOTAL
was
determined
HPLC
method,
parameter
oxidative
stress,
thiobarbituric
acid
reactive
substances
(TBARS),
spectrophotometric
platelets
isolated
from
whole
blood
high-resolution
respirometry.
had
lower
concentrations
blood,
plasma,
vs.
control
group.
Mitochondrial
CI-linked
LEAK
respiration
higher,
phosphorylation
(OXPHOS)
CII-linked
electron
transfer
(ET)
capacities
Plasma
TBARS
higher
H2
After
weeks
HRW,
CoQ10
increased,
plasma
decreased,
efficiency
OXPHOS
improved,
while
P
group,
changes
non-significant.
Long-term
supplementation
could
be
promising
strategy
acceleration
health
recovery
application
appears
new
treatment
targeted
disorders.
Additional
longer-term
studies
are
needed
confirm
elucidate
exact
mechanisms
mitochondria-targeted
effects
Cell Death and Disease,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: March 1, 2025
Abstract
Sepsis-associated
encephalopathy
(SAE)
is
a
severe
neurological
syndrome
marked
by
widespread
brain
dysfunctions
due
to
sepsis.
Despite
increasing
data
supporting
the
hypothesis
of
neuronal
damage,
exact
mechanism
sepsis-related
cognitive
disorders
and
therapeutic
strategies
remain
unclear
need
further
investigation.
In
this
study,
sepsis
model
was
established
in
C57
mice
using
lipopolysaccharide
(LPS).
The
findings
demonstrated
that
LPS
exposure
induced
loss,
synaptic
deficits
accompanied
mitochondrial
damage.
Bioinformatics
western
blot
analyses
significant
increase
Lipocalin-2
(LCN2)
during
as
key
hub
gene
involved
immune
inflammation.
Interestingly,
recombinant
LCN2
protein
exhibited
similar
effects
on
dysfunction
mice.
Conversely,
downregulating
effectively
nullified
impact
LPS,
leading
amelioration
deficits,
reactive
oxygen
species
(ROS)-associated
These
suggest
novel
etiopathogenic
SAE,
which
initiated
increased
LCN2,
loss
deficit.
Inhibition
could
be
therapeutically
beneficial
treating
sepsis-induced
impairments.
Biotechnology and Applied Biochemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 14, 2025
ABSTRACT
Senkyunolide
I
(Sen
I)
has
a
protective
effect
on
the
blood–brain
barrier
(BBB)
in
rats
with
sepsis‐associated
encephalopathy
(SAE).
This
study
investigated
whether
Sen
regulates
Nrf2
to
ameliorate
sepsis‐induced
brain
dysfunction
(SIBD).
Sixty
were
randomly
assigned
into
Sham
group,
SAE
group
(Model
group),
+
(72
mg/kg,
and
SAE+
positive
control
(RTA
402,
receptor
agonist,
RTA
402
15
each
group.
The
cecal
ligation
puncture
(CLP)
method
was
applied
induce
sepsis
rats.
modeling
verified
6
h
after
operation.
drug
administered
24
surgery.
Six
sacrificed
administration,
brains
extracted.
remaining
would
continue
be
observed
for
their
survival
status
until
72
post‐surgery.
Brain
cell
apoptosis
measured
using
TUNEL.
We
detected
expression
of
glial
fibrillary
acidic
protein
(GFAP)
by
immunofluorescence,
gene
RT‐qPCR,
Nrf2,
MMP‐9,
AQP‐4,
occludin
Western
blot.
TNF‐α
IL‐1β
levels
tested
ELISA,
malondialdehyde
(MDA)
glutathione
peroxidase
(GSH‐Px)
biochemical
tests.
Survival
rate
at
post‐surgery,
100%.
(44.4%)
(55.6%)
is
significantly
higher
than
that
Model
(11.1%).
Both
can
improve
tissue
damage
caused
sepsis,
specifically
reducing
GFAP
expression,
TNF‐α,
IL‐1β,
MDA
levels,
increasing
activity
GSH‐Px,
downregulating
MMP‐9
upregulating
occludin.
Moreover,
increased
rat
tissues.
ameliorates
SIBD
regulating
astrocyte
activation.
MedComm,
Journal Year:
2025,
Volume and Issue:
6(5)
Published: April 28, 2025
ABSTRACT
Molecular
hydrogen
(H
2
),
recognized
as
the
smallest
gas
molecule,
is
capable
of
permeating
cellular
membranes
and
diffusing
throughout
body.
Due
to
its
high
bioavailability,
H
considered
a
therapeutic
for
treatment
various
diseases.
The
efficacy
contingent
upon
factors
such
administration
method,
duration
contact
with
diseased
tissue,
concentration
at
targeted
sites.
can
be
administered
exogenously
also
produced
endogenously
within
intestinal
tract.
A
comprehensive
understanding
delivery
mechanisms
modes
action
crucial
advancing
medicine.
This
review
highlights
H₂’s
action,
summarizes
methods,
explores
advancements
in
treating
diseases
(e.g.,
inflammatory
bowel
disease,
ischemia–reperfusion,
colorectal
cancer).
Additionally,
applications
managing
other
are
discussed.
Finally,
challenges
associated
clinical
application
potential
solutions
explored.
We
propose
that
current
faced
by
effectively
addressed
through
use
nanoplatforms;
furthermore,
interactions
between
gut
microbiota
may
provide
insights
into
Future
research
should
explore
synergistic
effects
conjunction
conventional
therapies
develop
personalized
plans
achieve
precision
International Immunopharmacology,
Journal Year:
2023,
Volume and Issue:
123, P. 110758 - 110758
Published: Aug. 8, 2023
Sepsis-associated
encephalopathy
(SAE)
is
characterised
by
long-term
cognitive
impairment
and
psychiatric
illness
in
sepsis
survivors,
associated
with
increased
morbidity
mortality.
There
a
lack
of
effective
therapeutics
for
SAE.
Molecular
hydrogen
(H2)
plays
multiple
roles
septic
diseases
regulating
neuroinflammation,
reducing
oxidative
stress
parameters,
signalling
pathways,
improving
mitochondrial
dysfunction,
astrocyte
microglia
activation.
Here
we
report
the
protective
effect
hydrogen-rich
saline
juvenile
SAE
rat
model
its
possible
underlying
mechanisms.
Rats
were
injected
intraperitoneally
lipopolysaccharide
at
dose
5
mg/kg
to
induce
sepsis;
Hydrogen-rich
(HRS)
was
administered
1
h
after
LPS
induction
ml/kg
nigericin
before
injection.
H&E
staining
neuronal
damage,
TUNEL
assay
detection
apoptotic
cells,
immunofluorescence,
ELISA
protocol
inflammatory
cytokines
8-OHdG
determination
western
blot
analysis
determine
HRS
LPS-induced
rats.
treated
showed
decreased
TNF-α
IL-1β
expression
levels.
treatment
enhanced
activities
antioxidant
enzymes
(SOD,
CAT
GPX)
MDA
MPO
activities.
The
number
MMP-9
NLRP3
positive
immunoreactivity
cells
HRS-treated
group.
Subsequently,
GFAP,
IBA-1
CD86
reduced,
CD206
treatment.
Western
NLRP3,
ASC,
caspase-1,
MMP-2/9,
TLR4
Bax
protein
levels
treatment,
while
Bcl-2
These
data
demonstrated
that
attenuated
inflammasome
activation,
injury,
damage
via
NLRP3/Caspase-1/TLR4
model,
making
it
potential
therapeutic
agent
paediatric
