Biotechnology and Genetic Engineering Reviews,
Journal Year:
2023,
Volume and Issue:
40(4), P. 4361 - 4379
Published: May 8, 2023
We
investigate
the
impact
of
bovine
pulmonary
surfactant
(PS)
on
LPS-induced
ALI
in
vitro
and
vivo
to
improve
recognition
prevent
mortality
sepsis-induced
ALI.
Primary
alveolar
type
II
(AT2)
cells
were
treated
with
LPS
alone
or
combination
PS.
Cell
morphology
observation,
CCK-8
proliferation
assay,
flow
cytometry
apoptosis
ELISA
for
inflammatory
cytokine
levels
performed
at
different
time
points
after
treatment.
An
rat
model
was
established
vehicle
Lung
wet/dry
weight
ratio,
histopathological
changes,
lung
function
parameters,
serum
examined
6
h
PS
Survival
analysis
by
Kaplan-Meier
method.
RNA
sequencing
conducted
identify
differentially
expressed
genes
lungs.
Proapoptotic
gene
expression
lungs
determined
Western
blot.
significantly
inhibited
cell
while
promoting
AT2
starting
2
treatment,
accompanied
a
significant
increase
production;
reversed
these
effects.
decreased
ratio
septic
rats,
histological
abnormalities,
alterations
cytokines
improving
overall
survival
rats.
closely
associated
apoptosis.
attenuated
upregulation
proapoptotic
treatment
restoring
ATPase
activity
vivo.
Bovine
alleviates
early
phase,
possibly
suppressing
inflammation
apoptosis,
as
preemptive
therapeutic
agent
managing
Materials Today Bio,
Journal Year:
2024,
Volume and Issue:
28, P. 101247 - 101247
Published: Sept. 14, 2024
Hydrogen
gas
and
microalgae
both
exist
in
the
natural
environment.
We
aimed
to
integrate
hydrogen
biology
nano
together
expand
treatment
options
sepsis.
Biotechnology and Genetic Engineering Reviews,
Journal Year:
2023,
Volume and Issue:
40(4), P. 4361 - 4379
Published: May 8, 2023
We
investigate
the
impact
of
bovine
pulmonary
surfactant
(PS)
on
LPS-induced
ALI
in
vitro
and
vivo
to
improve
recognition
prevent
mortality
sepsis-induced
ALI.
Primary
alveolar
type
II
(AT2)
cells
were
treated
with
LPS
alone
or
combination
PS.
Cell
morphology
observation,
CCK-8
proliferation
assay,
flow
cytometry
apoptosis
ELISA
for
inflammatory
cytokine
levels
performed
at
different
time
points
after
treatment.
An
rat
model
was
established
vehicle
Lung
wet/dry
weight
ratio,
histopathological
changes,
lung
function
parameters,
serum
examined
6
h
PS
Survival
analysis
by
Kaplan-Meier
method.
RNA
sequencing
conducted
identify
differentially
expressed
genes
lungs.
Proapoptotic
gene
expression
lungs
determined
Western
blot.
significantly
inhibited
cell
while
promoting
AT2
starting
2
treatment,
accompanied
a
significant
increase
production;
reversed
these
effects.
decreased
ratio
septic
rats,
histological
abnormalities,
alterations
cytokines
improving
overall
survival
rats.
closely
associated
apoptosis.
attenuated
upregulation
proapoptotic
treatment
restoring
ATPase
activity
vivo.
Bovine
alleviates
early
phase,
possibly
suppressing
inflammation
apoptosis,
as
preemptive
therapeutic
agent
managing
