Tumor-infiltrating plasma cells are a prognostic factor in penile squamous cell carcinoma

Virchows Archiv, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

Language: Английский

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Tumor immune dysfunction and exclusion subtypes in bladder cancer and pan-cancer: a novel molecular subtyping strategy and immunotherapeutic prediction model

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: April 17, 2024

Molecular subtyping is expected to enable precise treatment. However, reliable strategies for clinical application remains defective and controversial. Given the significance of tumor immune dysfunction exclusion (TIDE), we aimed develop a novel TIDE-based strategy guide personalized immunotherapy in bladder cancer (BC).

Language: Английский

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UBE2J1 is identified as a novel plasma cell-related gene involved in the prognosis of high-grade serous ovarian cancer

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 28, 2025

Immune cells within tumor tissues play important roles in remodeling the microenvironment, thus affecting progression and therapeutic response. The current study was designed to identify key markers of plasma explore their role high-grade serous ovarian cancer (HGSOC). We utilized single-cell sequencing data from Gene Expression Omnibus (GEO) database immune cell types HGSOC extract related via Seurat package. effects on prognosis were analyzed univariate Cox regression, least absolute shrinkage selection operator (LASSO) gene set variation analysis (GSVA) bulk Cancer Genome Atlas (TCGA)-HGSOC cohort. Finally, evaluated Cell Counting Kit-8 (CCK-8), Transwell, colony formation, wound healing, immunofluorescence vivo growth assays. At level, we detected a significant increase proportion samples compared that normal samples. Within tissues, these found interact with CD8 + T cells, fibroblasts endothelial cells. In addition, patients high-plasma cell-related score group had better survival rates higher epithelial‒mesenchymal transition (EMT), apoptosis scores. Moreover, LASSO regression analyses revealed ubiquitin-conjugating enzyme E2 J1 (UBE2J1) is prognostic marker HGSOC. Further functional studies overexpression UBE2J1 promoted proliferation, invasion, migration whereas knockdown attenuated abovementioned cellular behaviors. Additionally, EMT, as evidenced by alterations protein expression levels N-cadherin, snail family transcriptional repressor 2 (Slug), Twist BHLH transcription factor 1 (Twist 1) E-cadherin. silencing able inhibit vivo. Overall, this elucidated novel oncogene HGSOC, uncovering new mechanisms tumorigenesis promising targets for patients.

Language: Английский

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Immunometabolism of ferroptosis in the tumor microenvironment

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: Aug. 12, 2024

Ferroptosis is an iron-dependent form of cell death that results from excess lipid peroxidation in cellular membranes. Within the last decade, physiological and pathological roles for ferroptosis have been uncovered autoimmune diseases, inflammatory conditions, infection, cancer biology. Excitingly, metabolism may be targeted to induce by cancers are resistant other forms death. sensitivity regulated oxidative stress, metabolism, iron which all influenced tumor microenvironment (TME). Whereas some types shown adapt these stressors, it not clear how immune cells regulate their sensitivities ferroptosis. In this review, we discuss mechanisms different subsets, influences infiltrate TME, interactions can determine epithelial-to-mesenchymal transition (EMT) metastasis. While much focus has placed on inducing cells, important considerations ferroptosis-modulating strategies impact anti-tumor immunity. From perspective, also promising immunotherapies field challenges associated with targeting specific populations.

Language: Английский

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Re-analysis of single cell and spatial transcriptomics data reveals B cell landscape in gastric cancer microenvironment and its potential crosstalk with tumor cells for clinical prognosis

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Aug. 30, 2024

At present, immunotherapy has become a powerful treatment for advanced gastric cancer (AGC), but not all patients can benefit from it. According to the latest research, impact of B cell subpopulations on immune microenvironment (GC) is unknown. Exploring whether interaction between cells and tumor in GC affects effectiveness attracted our interest.

Language: Английский

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New Perspectives on the Role of Liquid Biopsy in Bladder Cancer: Applicability to Precision Medicine

Cancers, Journal Year: 2024, Volume and Issue: 16(4), P. 803 - 803

Published: Feb. 16, 2024

Bladder cancer (BC) is one of the most common tumors in world. Cystoscopy and tissue biopsy are standard methods screening early diagnosis suspicious bladder lesions. However, they invasive procedures that may cause pain infectious complications. Considering limitations both procedures, recurrence resistance to BC treatment, it necessary develop a new non-invasive methodology for multiple evaluations patients under follow-up cancer. In recent years, liquid has proven be very useful diagnostic tool detection tumor biomarkers. This technique makes possible analyze single components released into peripheral circulation monitor progression. Numerous biomarkers being studied interesting clinical applications these presented, with promising results diagnosis, microscopic disease, prediction response treatment.

Language: Английский

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Targeted Delivery of Catalase and Photosensitizer Ce6 by a Tumor-Specific Aptamer Is Effective against Bladder Cancer In Vivo

Molecular Pharmaceutics, Journal Year: 2024, Volume and Issue: 21(4), P. 1705 - 1718

Published: March 11, 2024

Photodynamic therapy (PDT) is often applied in a clinical setting to treat bladder cancer. However, current photosensitizers report drawbacks such as low efficacy, selectivity, and numerous side effects, which have limited the values of PDT for Previously, we developed first cancer-specific aptamer that can selectively bind be internalized by tumor cells versus normal uroepithelium cells. Here, use an aptamer-based drug delivery system deliver photosensitizer chlorine e6 (Ce6) into In addition Ce6, also incorporate catalase complex increase local oxygen levels tissue. Compared with free aptamer-guided DNA nanotrain (NT) loaded Ce6 (NT–Catalase–Ce6) specifically recognize cancer cells, produce locally, induce ROS cause mitochondrial apoptosis. orthotopic mouse model cancer, intravesical instillation NT–Catalase–Ce6 exhibits faster internalization longer retention time tissue compared urothelium. Moreover, our modified significantly inhibits growth fewer effects cystitis than Ce6. This therefore improve selectivity efficacy reduce treatment models bearing great translational value therapy.

Language: Английский

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Single-cell RNA sequencing and spatial transcriptome analysis in bladder cancer: Current status and future perspectives

Bladder Cancer, Journal Year: 2025, Volume and Issue: 11(1)

Published: Jan. 1, 2025

Background Bladder cancer is one of the most prevalent malignancies, and mechanisms underlying its progression role tumor microenvironment (TME) are unclear. Recent advancements in single-cell RNA sequencing (scRNA-seq) spatial transcriptomics (ST) enable detailed analysis cellular heterogeneity, gene expression, cell–cell interactions bladder diseases. Methodology We conducted a comprehensive search for recent articles that have investigated diseases using scRNA-seq ST. Results ST led to significant discoveries disease research. These technologies enabled identification multiple molecular subtypes within individual tumors treatment resistance. Additionally, differences based on gender been explored, explaining heterogeneity incidence cancer. findings deepen our understanding pathology highlight transformative potential identifying novel biomarkers therapeutic targets. Conclusions Integrating has considerably enhanced tissues. insights may lead development personalized therapies improvement patient outcomes. Several challenges, such as technical limitations access difficulties, need be addressed future clinical application these technologies.

Language: Английский

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Analysis of single-cell and spatial transcriptomics in TNBC cell-cell interactions

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 26, 2025

Triple-negative breast cancer (TNBC) is a highly malignant tumor in women, characterized by high morbidity, mortality, and recurrence rates. Although surgical treatment, radiotherapy, chemotherapy are the mainstays of current treatment methods, heterogeneity TNBC results unsatisfactory outcomes with low 5-year survival Rapid advancements omics technology have propelled understanding molecular biology. The emergence single-cell RNA sequencing (scRNA-seq) spatial transcriptomics (ST) has significantly enhanced knowledge distribution, functionality, intercellular interactions various cell types within microenvironment, including cells, T B macrophages, fibroblasts. present study provides an overview technical characteristics scRNA-seq ST, highlighting their applications exploring heterogeneity, distribution patterns, interactions. This review aims to enhance comprehension at cellular level for development effective therapeutic targets.

Language: Английский

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Single-cell and spatial transcriptomic analysis reveals tumor cell heterogeneity and underlying molecular program in colorectal cancer

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 12, 2025

Background Colorectal cancer (CRC) is a highly heterogeneous tumor, with significant variation in malignant cells, posing challenges for treatment and prognosis. However, this heterogeneity offers opportunities personalized therapy. Methods The consensus non-negative matrix factorization algorithm was employed to analyze single-cell transcriptomic data from CRC, which helped identify cell expression programs (MCEPs). Subsequently, crosstalk network linking MCEPs immune/stromal trajectory development constructed using Monocle3 NicheNet. Additionally, bulk RNA-seq were utilized systematically explore the relationships between MCEPs, clinical features, genetic mutations. A prognostic model then established through Lasso Cox regression analyses, integrating into nomogram risk prediction. Furthermore, key genes associated their potential therapeutic targets identified protein-protein interaction networks, followed by molecular docking predict drug-binding affinity. Results We classified CRC transcriptional states eight distinct successfully networks these immune or stromal cells. containing 15 developed, demonstrating an AUC greater than 0.8 evaluation over 1 10 years when combined features. drug target gene TIMP1 identified, several targeted drugs discovered. Conclusion This study demonstrated that characterization of could effectively reveal biological features tumors like exhibit tumor prognosis assessment. Our research provides new theoretical practical directions

Language: Английский

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