Biochemical Pharmacology, Journal Year: 2024, Volume and Issue: unknown, P. 116699 - 116699
Published: Dec. 1, 2024
Language: Английский
Journal of Biochemical and Molecular Toxicology, Journal Year: 2025, Volume and Issue: 39(4)
Published: March 21, 2025
Circular RNAs (circRNAs) play critical roles in human tumorigenesis. Circ_0004104 is abnormally expressed the tumors of colorectal cancer (CRC). However, its specific function CRC remains unknown. In this report, we explored biological action and mechanism circ_0004104 development. Quantitative real-time PCR was used to detect circ_0004104, microRNA (miR)-493-5p, synaptophysin-like 1 (SYPL1) mRNA levels. Fluorescence situ hybridization (FISH) assay visualize circ_0004104. The impact on cell phenotypes assessed by measuring proliferation, migration, invasion, apoptosis. Animal experiments were performed analyze effect xenograft growth vivo. potential interacting miRNAs predicted using RNA Interactome database, binding sites for miR-493-5p SYPL1 Starbase3.0 database. circ_0004104/miR-493-5p miR-493-5p/SYPL1 relationships validated dual-luciferase reporter assay. tissues lines, levels upregulated expression decreased. mainly located cytoplasm cells exhibited resistance RNase R digestion. High a poor prognosis patients. Functionally, knockdown suppressed invasiveness accelerated apoptosis vitro, as well diminished tumor depletion also decreased N-cadherin Vimentin increased E-cadherin cells. Mechanistically, could act sponge, direct target miR-493-5p. Moreover, targeted regulate expression. effects behavior alterations reversed inhibitor. Additionally, overexpression phenotypes. Our findings suggest that enhances malignant progression through cascade, providing treatment.
Language: Английский
Citations
0
World Journal of Gastrointestinal Oncology, Journal Year: 2025, Volume and Issue: 17(4)
Published: March 24, 2025
Gastrointestinal (GI) cancers, which predominantly manifest in the stomach, colorectum, liver, esophagus, and pancreas, accounting for approximately 35% of global cancer-related mortality. The advent liquid biopsy has introduced a pivotal diagnostic modality early identification premalignant GI lesions incipient cancers. This non-invasive technique not only facilitates prompt therapeutic intervention, but also serves as critical adjunct prognosticating likelihood tumor recurrence. wealth circulating exosomes present body fluids is often enriched with proteins, lipids, microRNAs, other RNAs derived from cells. These specific cargo components are reflective processes involved tumorigenesis, progression, response to treatment. As such, they represent group promising biomarkers aiding diagnosis cancer. In this review, we delivered an exhaustive overview composition pathways sorting within these vesicles. We laid out some clinical evidence that supported utilization cancers discussed their potential application. Furthermore, addressed challenges encountered when harnessing predictive instruments realm
Language: Английский
Citations
0
Cell Death Discovery, Journal Year: 2025, Volume and Issue: 11(1)
Published: April 24, 2025
Abstract Breast cancer (BC) poses significant challenges globally, necessitating a deeper understanding of its complexities. Exosomes are cell-specific secreted extracellular vesicles interest, characterized by lipid bilayer structure. can carry variety bioactive components, including nucleic acids, lipids, amino and small molecules, to mediate intercellular signaling. CircRNAs novel class single-stranded RNA closed-loop mainly exert ceRNA functions intricately modulate gene expression signaling pathways in breast cancer, influencing tumor progression therapeutic responses. The unique packaging circRNAs within exosomes serves as genetic information transmitters, facilitating communication between BC cells microenvironmental cells, thereby regulating critical aspects progression, immune evasion, drug resistance. Besides, exosomal possess the capabilities serving diagnostic biomarkers BC, due their stability, specificity, regulatory roles tumorigenesis metastasis. Therefore, this review aims elucidate mechanisms well potential for diagnosis therapeutics. ongoing investigations will potentially revolutionize treatment paradigms improve patient outcomes BC.
Language: Английский
Citations
0
Human Reproduction, Journal Year: 2024, Volume and Issue: 39(8), P. 1608 - 1617
Published: June 17, 2024
Since the first description of adenomyosis more than 150 years ago, multiple hypotheses have attempted to explain its pathogenesis. Indeed, research over recent has greatly enhanced our knowledge underlying causes. This opened up avenues for development strategies both disease prevention and treatment main symptoms, such as pelvic pain, heavy menstrual bleeding, infertility. However, current means are still largely ineffective, so it is vital that we shed light on pathways involved. Dysregulated mechanisms aberrant protein expression been identified contributing factors in interactions between endometrial epithelial stromal cells, ultimately leading growth adenomyotic lesions. These include collective cell migration, epithelial-to-mesenchymal transition, hormonal influence, signaling from non-coding RNAs extracellular vesicles. We provide a concise summary latest insights into crosstalk glands stroma ectopic lesion formation. While there an abundance literature similarities deep endometriosis, insufficient data cytochemical, molecular, pathogenetic these two disorders. various shared features, including alterations adhesion molecules, abnormal hormone regulation, presence cancer-driving mutations epigenetic modifications, identified. Nevertheless, pathogenic contribute cause enigmatic diseases not fully elucidated yet.
Language: Английский
Citations
3
Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12
Published: Jan. 25, 2024
Gastric cancer (GC) is a tumor characterized by high incidence and mortality, with metastasis being the primary cause of poor prognosis. Extracellular vesicles (EVs) are an important intercellular communication medium. They contain bioactive substances such as proteins, nucleic acids, lipids. EVs play crucial biological role in process GC metastasis. Through mechanisms remodeling microenvironment (TME), immune suppression, promoting angiogenesis, facilitating epithelial–mesenchymal transition (EMT) mesothelial–mesenchymal (MMT), promote invasion GC. Further exploration roles will contribute to our understanding underlying may provide novel targets strategies for diagnosis treatment In this review, we summarize which influence from four aspects: TME, modulating system, influencing processes EMT MMT. Finally, briefly summarized organotropism well potential limitations
Language: Английский
Citations
2
Biocell, Journal Year: 2024, Volume and Issue: 48(4), P. 571 - 580
Published: Jan. 1, 2024
Objective: Previous studies indicated that aberrant circular RNA (circRNA) expression affects gene regulatory networks, leading to the activation of tumor pathways and promoting cell growth.However, expression, clinical significance, effects on propagation, invasion, dissemination circRNA_001896 in cervical cancer (CC) tissues remain unclear.Methods: The Gene Expression Omnibus (GEO) datasets (GSE113696 GSE102686) were used examine differential circRNA CC adjacent tissues.The was detected 72 patients using fluorescence quantitative PCR.Correlation analysis with pathological features performed through COX multivariate univariate analysis.The effect downregulation propagation examined counting kit-8 (CCK-8) test, clonogenic, 3D sphere formation, vivo tumorigenesis assays.Results: Intersection GSE113696 GSE102686 revealed an increased four circRNAs, including circRNA_001896, tissues.Fluorescence PCR confirmed as a RNA.High considerably associated lymph node metastasis, International Federation Gynecologists Obstetricians (FIGO) stage, diameter, survival period patients.Proportional hazards model (COX) analyses expressions are distinct risk factor affecting patients' prognosis.Cellular functional experiments showed downregulating substantially suppressed growth, colony sphere-forming ability.In vivo, nude mice demonstrated remarkably reduced proliferation capacity cells. Conclusion:CircRNA_001896 is highly expressed related FIGO size, patients.Moreover, significantly inhibits both vitro cells.Therefore, might be biomarker for targeted therapy cancer.
Language: Английский
Citations
2
Non-coding RNA Research, Journal Year: 2024, Volume and Issue: 9(4), P. 1280 - 1291
Published: May 20, 2024
Ovarian cancer (OC) is the most common cause of death in female cancers. The prognosis OC very poor due to delayed diagnosis and identification patients advanced stages, metastasis, recurrence, resistance chemotherapy. As chemotherapy with platinum-based drugs such as cisplatin (DDP) main treatment cases, DDP an important obstacle achieving satisfactory therapeutic efficacy. Consequently, knowing different molecular mechanisms involved necessary achieve new approaches. According numerous recent studies, non-coding RNAs (ncRNAs) could regulate proliferation, differentiation, apoptosis, chemoresistance many cancers, including OC. Most these ncRNAs are released by tumor cells into human fluid, allowing them be used tools for diagnosis. CircRNAs ncRNA family members that have a role initiation, progression, regulation various In current study, we investigated roles several circRNAs their signaling pathways on progression also during
Language: Английский
Citations
2
Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14
Published: June 12, 2024
Squamous cell carcinoma (SCC) is a prevalent malignancy affecting multiple organs in the human body, including oral cavity, esophagus, cervix, and skin. Given its significant incidence mortality rates, researchers are actively seeking effective diagnostic therapeutic strategies. In recent years, exosomes their molecular cargo, particularly circular RNA (circRNA), have emerged as promising areas of investigation SCC research. Exosomes small vesicles released into extracellular environment by cells that contain biomolecules reflect physiological state origin. CircRNAs, known for unique covalently closed loop structure stability, garnered special attention oncology closely associated with tumorigenesis, progression, metastasis, drug resistance. Interestingly, exosomal circRNAs been identified ideal biomarkers noninvasive cancer diagnosis prognosis assessment. This article reviews progress research on circRNAs, focusing expression patterns, functions, potential applications SCC, aiming to provide new insights strategies treatment SCC.
Language: Английский
Citations
2
Journal of Pharmaceutical Analysis, Journal Year: 2024, Volume and Issue: 15(2), P. 101067 - 101067
Published: Aug. 13, 2024
Exosomal circRNAs are pivotal in cancer biology, and tumor pathophysiology. These stable, non-coding RNAs encapsulated exosomes participated progression, growth, metastasis, drug sensitivity the microenvironment (TME). Their presence bodily fluids positions them as potential non-invasive biomarkers, revealing molecular dynamics of cancers. Research exosomal is reshaping our understanding neoplastic intercellular communication. Exploiting natural properties for targeted delivery disrupting circRNA-mediated pro-tumorigenic signaling can develop new treatment modalities. Therefore, ongoing exploration research poised to revolutionize clinical management cancer. This emerging field offers hope significant breakthroughs care. review underscores critical role biology resistance, highlighting their biomarkers therapeutic targets that could transform
Language: Английский
Citations
2
Oncology Reports, Journal Year: 2023, Volume and Issue: 51(2)
Published: Dec. 8, 2023
Gastrointestinal cancer is frequently detected at an advanced stage and has undesirable prognosis due to the absence of efficient precise biomarkers therapeutic targets. Exosomes are small, living‑cell‑derived vesicles that serve a critical role in facilitating intercellular communication by transporting molecules from donor cells receiver cells. circular RNAs (circRNAs) mis‑expressed variety diseases, including gastrointestinal cancer, promising as diagnostic tumor targets for cancer. The main features exosomes circRNAs discussed present review, along with research on biological function exosomal development progression It also assesses advantages disadvantages implementing these findings clinical applications.
Language: Английский
Citations
5