VEGF-B/NRP1 Signaling Modulates Mitochondrial Homeostasis and Cardiac Function After Myocardial Infarction DOI Creative Commons

Sai Manasa Varanasi,

Ankit Sabharwal,

Riya Kar

et al.

Published: April 20, 2025

Abstract Background Myocardial infarction (MI) remains a leading cause of mortality worldwide. Recent studies suggest cardioprotective role for vascular endothelial growth factor-B (VEGF-B) in MI. However, the molecular mechanisms VEGF-B-mediated signaling via its co-receptor Neuropilin-1 (NRP1) MI are poorly understood. In this study, we investigated intricate involving VEGF-B and NRP1 cardiomyocytes (CMs) using ischemic injury as model And secondly, further validated protective VEFG-B heart disease, shedding light on their roles not only cardiac function but also therapeutic potential. Methods utilized both vitro vivo approaches to elucidate how it manifests mitochondrial functions regeneration following injury. We used two different cardiomyocyte cell lines, H9c2 (rat ventricular cardiomyocytes) HL-1 (mouse cardiomyocytes), induced hypoxia conditions, either 1% oxygen or 200µM cobalt chloride (CoCl 2 ) mimic myocardial infarction-induced heart. addition, developed novel heat shock inducible zebrafish cardiomyocyte-specific overexpression system examine . Results Our findings indicate that predominantly expressed tissue compared other tissues, expression is altered response hypoxia/ischemic results demonstrate treatment prior enhances survival, while knockdown abolishes effect, highlighting prominent cardio protection. Furthermore, found promotes survival by improving function, evidenced reduced oxidative stress ROS accumulation, decreased stress, preserved membrane potential, increased ATP levels. Lastly, our transgenic model, demonstrated protects from an NRP1-dependent manner. Conclusion study has uncovered important VEGF-B-NRP1 axis mediated beneficial CMs. Importantly, against Novelty Significance What Is Known? dysfunction, death, adverse remodeling. Vascular Endothelial Growth Factor B traditionally associated with metabolism, biology, particularly angiogenesis lipid metabolism. The neurophilin-1 receptor, VEGF-B, implicated pathways. Emerging evidence suggests may influence integrity under conditions. New Information Does This Article Contribute? exerts effect mitocondria preserving reducing stress. receptor key mediator modulates homeostasis cardiomyocytes. can attenuate apoptosis enhance bioenergetics post-MI, suggesting mechanism. Clinical Implication: These strategies aimed at enhancing VEGF-B/NRP1 improve recovery after MI, offering avenue limiting failure progression disease.

Language: Английский

Dose–response association between Chinese visceral adiposity index and cardiovascular disease: a national prospective cohort study DOI Creative Commons
Yongcheng Ren, Qing Hu, Zheng Li

et al.

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: April 18, 2024

Background Chinese visceral adiposity index (CVAI) is a reliable obesity index, but the association between CVAI and risk of cardiovascular disease (CVD) remains unclear. We explored associations with incident CVD, heart disease, stroke compared predictive power other indices based on national cohort study. Methods The present study included 7,439 participants aged ≥45 years from China Health Retirement Longitudinal Study (CHARLS). Cox regression models were applied to estimate hazard ratios (HRs) 95% confidence intervals (CIs). Restricted cubic splines analyses adopted model dose–response associations. Receiver operator characteristic (ROC) used compare ability different (CVAI, [VAI], body shape [ABSI], conicity [CI], waist circumference [WC], mass [BMI]). Results During 7 years’ follow‐up, 1,326 1,032 399 cases identified. HRs (95% CI) 1.50 (1.25-1.79), 1.29 (1.05-1.57), 2.45 (1.74-3.45) for quartile 4 versus 1 in CVAI. Linear observed ( P nonlinear > 0.05) per-standard deviation (SD) increase was associated 17% (HR 1.17, 1.10-1.24), 12% (1.12, 1.04-1.20), 31% (1.31, 1.18-1.46) increased risk, respectively. Per-SD conferred higher aged<60 than those ≥60 interaction< 0.05). ROC showed that had value P< Conclusions linearly best performance predicting CVD. Our findings indicate as applicable identify

Language: Английский

Citations

17
The Role of Antioxidants in the Therapy of Cardiovascular Diseases—A Literature Review DOI Open Access
Ewelina Młynarska, Joanna Hajdys, Witold Czarnik

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(16), P. 2587 - 2587

Published: Aug. 6, 2024

Antioxidants are endogenous and exogenous substances with the ability to inhibit oxidation processes by interacting reactive oxygen species (ROS). ROS, in turn, small, highly capable of oxidizing a wide range molecules human body, including nucleic acids, proteins, lipids, carbohydrates, even small inorganic compounds. The overproduction ROS leads oxidative stress, which constitutes significant factor contributing development disease, not only markedly diminishing quality life but also representing most common cause death developed countries, namely, cardiovascular disease (CVD). aim this review is demonstrate effect selected antioxidants, such as coenzyme Q10 (CoQ10), flavonoids, carotenoids, resveratrol, well introduce new antioxidant therapies utilizing miRNA nanoparticles, reducing incidence progression CVD. In addition, context aforementioned diseases will be considered. This emphasizes pleiotropic effects benefits stemming from presence mentioned organism, leading an overall reduction risk, coronary heart dyslipidaemia, hypertension, atherosclerosis, myocardial hypertrophy.

Language: Английский

Citations

10
Climate-smart livestock nutrition in semi-arid Southern African agricultural systems DOI Creative Commons
F. Fushai, Teedzai Chitura, O.E. Oke

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 11, 2025

Climate change is disrupting the semi-arid agricultural systems in Southern Africa, where livestock crucial to food security and livelihoods. This review evaluates bioenergetic agroecological scope for climate-adaptive nutrition region. An analysis of literature on climate implications thermal welfare regional context was conducted. The information gathered systematically synthesized into tabular summaries fundamentals climate-smart bioenergetics, thermoregulation, heat stress defence mechanisms, thermo-bioactive feed components, potentially resources supports adoption when conceptualized as precision feeding combined with dietary strategies that enhance resilience livestock, adaptation production decline availability conventional feedstuffs by incorporating alternatives. keystone potential alternative are identified be small cereal grains, such sorghum (Sorghum bicolor) pearl millet (Pennisetum glaucum) energy sources, native legumes, cowpea (Vigna unguiculata) marama bean (Tylosema esculentum) protein wild browse Fabaceae trees Vachellia spp. Colophospermum mopane, which provide dry season drought supplementary protein, minerals, antioxidants, non-fabaceous tree species marula (Sclerocarya birrea), from animals consume electrolyte-rich fresh fruit or processed pulp. Feedstuffs circular include oilseed cakes macadamia (Macadamia integrifolia) nut, castor (Ricinus communis), Jatropha (Jatropha curcas) beans, rich energy, insect primarily black soldier fly larvae (Hermetia illucens), microbial phototrophic algae (Spirulina, Chlorella), yeasts (Saccharomyces cerevisiae). Additives thermo-functionally enhanced diets synthetic natural anti-oxidants, phytogenics, biotic agents (prebiotics, probiotics, synbiotics, postbiotics), electrolytes. presents a conceptual framework system across livestock-energy-water-food nexus, inform broader, in-depth research, promote farm practices support governmental policies tailored agroecology

Language: Английский

Citations

1
Cucurbitacin B induces ferroptosis in oral leukoplakia via the SLC7A11/mitochondrial oxidative stress pathway DOI Creative Commons
Mengyuan Yang, Xin Chen, Cheng Chen

et al.

Phytomedicine, Journal Year: 2024, Volume and Issue: 129, P. 155548 - 155548

Published: March 20, 2024

Oral leukoplakia (OLK), characterized by abnormal epithelial hyperplasia, is the most common precancerous oral mucosa lesion and closely related to oxidative stress. Cucurbitacin B (CuB), a tetracyclic triterpenoid molecule derived from plants, has shown promising anti-proliferative antioxidant effects in preclinical studies. However, whether CuB can play an antiproliferative role OLK regulating stress remains elusive. To investigate of inhibiting malignant progression further explore its underlying mechanisms action. In vitro, effect on proliferation, migration, apoptosis, cell cycle cells DOK was detected. The core genes key pathways were analyzed transcriptome database, using immunofluorescence, qRT-PCR, Western blot evaluate expression levels ferroptosis markers ROS, GSH, MDA, Fe2+, marker SLC7A11, GPX4, FTH1. Immunohistochemistry human tissue performed SLC7A11. vivo, model established C57BL/6 mice biosafety treatment for evaluated. substantially suppressed proliferation cells. Bioinformatics analysis showed that targets crossing with include SLC7A11 essential involve ROS ferroptosis. vitro experiments indicated might promote down-regulating We observed gradual increase during normal varying degrees dysplasia. vivo demonstrated inhibited promoting exhibited certain level biosafety. This study first time could effectively inhibit inducing via activating SLC7A11/ mitochondrial pathway. These findings indicate serve as lead compound future development anti-oral drugs.

Language: Английский

Citations

7
Interactive association of the dietary oxidative balance score and cardiovascular disease with mortality in older adults: evidence from NHANES DOI

Huanrui Zhang,

Wen Tian,

Guoxian Qi

et al.

Food & Function, Journal Year: 2024, Volume and Issue: 15(11), P. 6164 - 6173

Published: Jan. 1, 2024

: We conducted an assessment to explore potential associations of the dietary oxidative balance score (DOBS), cardiovascular disease (CVD), with all-cause mortality among older adults, while also exploring moderating effect DOBS on relationship between CVD and mortality.

Language: Английский

Citations

6
Multi-omics analysis reveals attenuation of cellular stress by empagliflozin in high glucose-treated human cardiomyocytes DOI Creative Commons
Lucia Scisciola, Ugo Chianese, Vicky Caponigro

et al.

Journal of Translational Medicine, Journal Year: 2023, Volume and Issue: 21(1)

Published: Sept. 23, 2023

Sodium-glucose cotransporter 2 (SGLT2) inhibitors constitute the gold standard treatment for type diabetes mellitus (T2DM). Among them, empagliflozin (EMPA) has shown beneficial effects against heart failure. Because cardiovascular diseases (mainly diabetic cardiomyopathy) are leading cause of death in patients, use EMPA could be, simultaneously, cardioprotective and antidiabetic, reducing risk from causes decreasing hospitalization failure T2DM patients. Interestingly, recent studies have that positive benefits people with without diabetes. This finding broadens scope function beyond glucose regulation alone to include a more intricate metabolic process is, part, still unknown. Similarly, this significantly increases number who may be eligible treatment.This study aimed clarify effect on human myocardial cell model by using orthogonal metabolomics, lipidomics, proteomics approaches. The untargeted multivariate analysis mimicked fasting blood sugar level patients (hyperglycemia: HG) average range (normal glucose: NG), addition EMPA.Results highlighted was able modulate partially restore levels multiple metabolites associated cellular stress, which were dysregulated HG conditions, such as nicotinamide mononucleotide, glucose-6-phosphate, lactic acid, FA 22:6 well nucleotide sugars purine/pyrimidines. Additionally, regulated several lipid sub-classes, particular dihydroceramide triacylglycerols, tend accumulate conditions resulting lipotoxicity. Finally, counteracted dysregulation endoplasmic reticulum-derived proteins involved stress management.These results suggest an different routes, tending rescue cardiomyocyte status towards healthy phenotype.

Language: Английский

Citations

12
Targeting of mitochondrial fission through natural flavanones elicits anti-myeloma activity DOI Creative Commons

Roberta Torcasio,

Maria Eugenia Gallo Cantafio,

Claudia Veneziano

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Feb. 27, 2024

Abstract Background Mitochondrial alterations, often dependent on unbalanced mitochondrial dynamics, feature in the pathobiology of human cancers, including multiple myeloma (MM). Flavanones are natural flavonoids endowed with targeting activities. Herein, we investigated capability Hesperetin (Hes) and Naringenin (Nar), two aglycones Hesperidin Naringin flavanone glycosides, to selectively target Drp1, a pivotal regulator prompting anti-MM activity. Methods Molecular docking analyses were performed crystallographic structure Dynamin-1-like protein (Drp1), using Hes Nar molecular structures. Cell viability apoptosis assessed MM cell lines, or co-culture systems primary bone marrow stromal cells, Titer Glo Annexin V-7AAD staining, respectively; clonogenicity was determined methylcellulose colony assays. Transcriptomic carried out Ion AmpliSeq™ platform; mRNA expression levels by quantitative RT-PCR western blotting, respectively. architecture transmission electron microscopy. Real time measurement oxygen consumption high resolution respirometry living cells. In vivo anti-tumor activity evaluated NOD-SCID mice subcutaneously engrafted Results found accommodate within GTPase binding site inhibit Drp1 activity, leading hyperfused mitochondria reduced OXPHOS. vitro, viability, even presence patient-derived triggering ER stress apoptosis. Interestingly, rewired metabolism through down-regulation master transcriptional activators (SREBF-1, c-MYC) lipogenesis genes. An extract Tacle, Citrus variety rich Naringin, capable recapitulate phenotypic perturbations each flavanone, vivo. Conclusion proliferation, rewire induce cells via antagonism fission driver Drp1. These results provide framework for development therapeutics aberrant dependencies.

Language: Английский

Citations

4
Therapeutic potentials of allicin in cardiovascular disease: advances and future directions DOI Creative Commons
Yijie Gao, Baofu Wang,

Gaofeng Qin

et al.

Chinese Medicine, Journal Year: 2024, Volume and Issue: 19(1)

Published: July 2, 2024

Abstract Cardiovascular disease (CVD) remains the predominant cause of mortality and disability worldwide. Against this backdrop, finding effective drugs for pharmacological treatment CVD has become one most urgent challenging issues in medical research. Garlic ( Allium sativum L.) is oldest plants world-renowned its dietary medicinal values. Allicin diallyl thiosulfinate ) primary natural active ingredients garlic, which been proven to have powerful cardioprotective effects mediate various pathological processes related CVD, such as inflammatory factor secretion, myocardial cell apoptosis, oxidative stress, more. Therefore, allicin holds a promising application prospect CVD. This review summarized biological functions potential mechanisms including antioxidation, anti-inflammation, anti-apoptosis effects. Reckoning with these, we delved into recent studies on allicin’s concerning CVDs, atherosclerosis, hypertension, infarction, arrhythmia, cardiac hypertrophy, heart failure, cardiotoxicity. Further, considering tremendous advancement nanomedicine, nanotechnology-based drug delivery systems show promise addressing limitations clinical applications, improving solubility, stability, bioavailability. Through review, hope provide reference further research cardioprotection development. Graphical

Language: Английский

Citations

4
Impact of Coffee Consumption on Mortality in Patients with Cardiovascular and Cerebrovascular Diseases: A Cohort Study DOI
Huining Zhou, J Li, Chun Zhang

et al.

Nutrition Metabolism and Cardiovascular Diseases, Journal Year: 2025, Volume and Issue: unknown, P. 103855 - 103855

Published: Jan. 1, 2025

Language: Английский

Citations

0
Potential therapeutic value of dietary polysaccharides in cardiovascular disease: Extraction, mechanisms, applications, and challenges DOI
Qiqi Jin, Bin Lin, Lingeng Lu

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 296, P. 139573 - 139573

Published: Jan. 8, 2025

Language: Английский

Citations

0