Identification and experimental validation of diagnostic and prognostic genes CX3CR1, PID1 and PTGDS in sepsis and ARDS using bulk and single-cell transcriptomic analysis and machine learning DOI Creative Commons
Jie Jiang, Yan Chen, Yue Su

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 23, 2024

Background Sepsis is an uncontrolled reaction to infection that causes severe organ dysfunction and a primary cause of ARDS. Patients suffering both sepsis ARDS have poor prognosis high mortality. However, the mechanisms behind their simultaneous occurrence are unclear. Methods We acquired datasets from GEO Arrayexpress databases screened hub genes by WGCNA machine learning algorithm. For diagnosis prognosis, ROC curve survival analysis were used. performed GO, KEGG, GSEA, immune cell infiltration, drug prediction, molecular docking, transcription factor constructed PPI ceRNA networks explore these common Single-cell data compared profiles gene localization. Finally, RT-qPCR H&E staining confirmed reliability using PBMCs samples mouse models. Results identified 242 differentially expressed in showed turquoise module GSE95233 strongly linked while black GSE10474 associated with Using three methods (LASSO, random forest Boruta), we key CX3CR1, PID1 PTGDS. Models built them AUC values evaluations validated external datasets, accurately predicting further explored immunological landscape infiltration single-cell analysis. Then, ceRNA, predicted drugs docking analyzed. Ultimately, demonstrated differently human Conclusion This study signatures (CX3CR1, PTGDS) ARDS, patient samples. research may be valuable for identifying shared biological potential treatment targets diseases.

Language: Английский

Comparative evaluation of cigarette smoke and a heated tobacco product on microglial toxicity, oxidative stress and inflammatory response DOI Creative Commons
Alfio Distefano, Laura Orlando,

Konstantinos Partsinevelos

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Sept. 30, 2024

Language: Английский

Citations

4
Ionizing radiation-induced disruption of Rela-Bclaf1-spliceosome regulatory axis in primary spermatocytes causing spermatogenesis dysfunction DOI Creative Commons
Hongjian Zhou, Zhipeng Xu,

chun Jiu Jiang

et al.

Cell Communication and Signaling, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 31, 2025

Ionizing radiation (IR) poses a significant threat to male fertility by inducing substantial changes in the testis, yet mechanisms underlying IR-induced spermatogenesis disorders remain poorly understood, necessitating development of more effective radioprotective agents. We employed Bulk RNA-seq and single-cell (scRNA-seq) on Balb/c mice testes models following IR exposure assess cellular transcriptional alterations. Histological examination, sperm concentration motility analysis, Western blotting (WB), reverse transcription quantitative PCR (RT-qPCR) were used evaluate testicular injury. The therapeutic potential NF-κB agonists was investigated an disorder model. A 6 Gy dose induced suppressed spliceosome pathway, predominantly affecting cell abundance spermatogonia primary spermatocytes. Bioinformatics analysis revealed that splicing differentiation-related genes, thereby impairing differentiation ability Mechanistically, This disruption linked inhibition NF-κB/Rela Bclaf1 activity. Notably, found ameliorate this damage via upregulating spliceosome-related genes expression, normalizing patterns rescuing disorders. study reveals novel IR-mediated Rela-Bclaf1-spliceosome regulatory axis spermatocytes propose Rela as drug target for mitigating not only provides new insights further research into spermatogenic caused other factors, but also offers strategies developing agents cancer radiotherapy.

Language: Английский

Citations

0
Macrophage pyroptosis and its crucial role in ALI/ARDS DOI Creative Commons

Yuju Cai,

Luorui Shang,

Fangyuan Zhou

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 14, 2025

Acute lung injury(ALI)/acute respiratory distress syndrome(ARDS) is a severe clinical syndrome characterized by high morbidity and mortality, primarily due to injury. However, the pathogenesis of ALI/ARDS remains complex issue. In recent years, role macrophage pyroptosis in injury has garnered extensive attention worldwide. This paper reviews mechanism pyroptosis, discusses its ALI/ARDS, introduces several drugs intervening measures that can regulate influence progression ALI/ARDS. By doing so, we aim enhance understanding provide novel insights for treatment.

Language: Английский

Citations

0
Targeting SphK1/S1PR3 axis ameliorates sepsis-induced multiple organ injury via orchestration of macrophage polarization and glycolysis DOI

Dan Wang,

Xinwen Bi, Le Zhao

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Journal Year: 2024, Volume and Issue: 1872(1), P. 119877 - 119877

Published: Nov. 14, 2024

Language: Английский

Citations

2
Mechanisms and Research Progress of Traditional Chinese Medicine Regulating NF-κB in the Treatment of Acute Lung Injury/Acute Respiratory Distress Syndrome DOI Creative Commons

Wanzhao Zuo,

Fanian Tian,

Jia Ke

et al.

Chinese medicine and natural products, Journal Year: 2024, Volume and Issue: 04(03), P. e93 - e105

Published: Sept. 1, 2024

Abstract Acute lung injury (ALI) has multiple causes and can easily progress to acute respiratory distress syndrome (ARDS) if not properly treated. Nuclear factor κB (NF-κB) is a key pathway in the treatment of ALI/ARDS. By exploring relevance NF-κB pathogenesis this disease, it was found that disease mainly associated with inflammation, dysfunction endothelial barrier, oxidative stress, impaired clearance alveolar fluid, coagulation disorders. Traditional Chinese medicine (TCM) characteristics multitargeting, multipathway effects, high safety, which directly or indirectly affect This article summarizes mechanism strategies TCM recent years through intervention NF-κB-related signaling pathways for treating It provides an overview from perspectives herbal monomers, couplet medicines, injections, compounds, preparations, offering insights into prevention ALI/ARDS TCM.

Language: Английский

Citations

0
Sphingosine-1-phosphate receptor 3 promotes neuronal apoptosis via the TNF-α/caspase-3 signaling pathway after acute intracerebral hemorrhage DOI
Dengpan Song, Mengyuan Li,

Longxiao Zhang

et al.

Molecular and Cellular Neuroscience, Journal Year: 2024, Volume and Issue: 131, P. 103979 - 103979

Published: Nov. 22, 2024

Language: Английский

Citations

0
Identification and experimental validation of diagnostic and prognostic genes CX3CR1, PID1 and PTGDS in sepsis and ARDS using bulk and single-cell transcriptomic analysis and machine learning DOI Creative Commons
Jie Jiang, Yan Chen, Yue Su

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 23, 2024

Background Sepsis is an uncontrolled reaction to infection that causes severe organ dysfunction and a primary cause of ARDS. Patients suffering both sepsis ARDS have poor prognosis high mortality. However, the mechanisms behind their simultaneous occurrence are unclear. Methods We acquired datasets from GEO Arrayexpress databases screened hub genes by WGCNA machine learning algorithm. For diagnosis prognosis, ROC curve survival analysis were used. performed GO, KEGG, GSEA, immune cell infiltration, drug prediction, molecular docking, transcription factor constructed PPI ceRNA networks explore these common Single-cell data compared profiles gene localization. Finally, RT-qPCR H&E staining confirmed reliability using PBMCs samples mouse models. Results identified 242 differentially expressed in showed turquoise module GSE95233 strongly linked while black GSE10474 associated with Using three methods (LASSO, random forest Boruta), we key CX3CR1, PID1 PTGDS. Models built them AUC values evaluations validated external datasets, accurately predicting further explored immunological landscape infiltration single-cell analysis. Then, ceRNA, predicted drugs docking analyzed. Ultimately, demonstrated differently human Conclusion This study signatures (CX3CR1, PTGDS) ARDS, patient samples. research may be valuable for identifying shared biological potential treatment targets diseases.

Language: Английский

Citations

0